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The map label for this gene is tgs2 [H]

Identifier: 119870620

GI number: 119870620

Start: 4820099

End: 4821463

Strand: Direct

Name: tgs2 [H]

Synonym: Mkms_4591

Alternate gene names: 119870620

Gene position: 4820099-4821463 (Clockwise)

Preceding gene: 119870618

Following gene: 119870626

Centisome position: 84.01

GC content: 69.23

Gene sequence:

>1365_bases
ATGAAGCTGATCTCGCCGACCGATTCGATGTTCTTGCTCGTCGAGTCGCGGGAGCATCCCATGCATGTCGGCGGGCTGCA
GTTGTTCGAACCGCCGGACGGCATCTCGGGTGCGGACTTCCTGCGCGAGATCCACCAGGCGATGGTGGAGCAGACCGATT
TCCAGGAGACGTTCCGCAAGCATCCGGGCACGGTGCTCGGCGGGGTCGCCAACCTCGCCTGGGCCGTCGACGAGGACGTC
GACCTGGACTACCACCTGCGCCGGTCGGCGCTGCCGTCGCCGGGACGGGTGCGCGAGCTGCTGGAGCTGACGTCGCGGCT
GCACGGCACGCTGCTCGACCGGCACCGCCCCCTGTGGGAGGCCCACCTGGTCGAAGGGCTCAGCGACGGACGCTTCGCGG
TGTACGTGAAGTTCCACCATGCCCTCATCGACGGGGTGTCGGCGCTCAAGCTGACCCAGCGCACGCTGTCGACCGATCCG
GACGACCCGGAGGTCCGGGTGCCGTGGAACCTGCCGCCGAAGCGGCGCACCCGCGAACCGGAGTCGAAGTCGCTGCTGCG
CAGCGTCACCGACCGCGTGGGTTCGGTTGCCGCGCTTGCCCCTTCGACGTTCGGCCTGGCGCGTGCGGCGTTGCTCGAGC
AACAACTCCAGCTGCCGTTCGGTGCGCCGAAGACGATGTTCAACGTCCCGATCGGCGGCGCCCGCCGGGTGGCGGCGCAG
TCGTGGCCGCTGGAGCGCATCCGCTCGGTCAAACGGGCCACCGGCGCGACGGTCAACGACGTCATCCTCGCGATGTGCGC
CGGTGCGCTGCGGTTCTACCTCACCGAACAGAACGCGCTCCCGGATGCGCCGCTCATCGCGATGGTGCCGGTGAGCCTGC
GCACTGAGGCGGAGGCCGACTCGGGCGGGAACATGACCGGCGTCGTGCTGTGCAACCTCGCCACCGACGACGACGATCCG
GCCCGCCGCCTCGACACCATCAGCCTGTCGATGCGCAGGAACAAGCAGGTGTTCTCCCAGTTGCCGCGGGTGCAGCAGCT
GGCGCTGTCGGCGTCGATGATCGCGCCCCTGGGCCTCGGGGCGGTGCCCGGCTTCGTCTCGACCGCTCCCCCGCCGTTCA
ACATCGTGATCTCCAATGTGCCGGGCAACCAGGAGCCGATGTACTGGCGCGGCGCCCGCCTCGACGGGAACTACCCGTTC
TCCATCGCCCTCGACGGCCAGGCGCTCAACATCACGATGGCCAACAACGGGGACAACCTGGACTTCGGGCTGGTCGGCGC
CCGCGGCAGCGTGCCTCACCTGCAGCGGCTGCTCGGCCACCTCGAGGATTCGCTCAAGGAACTCGAGGTCGCCGTCGGGG
TGTGA

Upstream 100 bases:

>100_bases
TGACATCTCCGAGAAGTTCCCTGTCCTCGACAGGCAAAACGTTGCCCGGGCCCCGCAGTGGGCATACAAAGGAGATCCAC
GAACCCCGAAGGGACCCGCG

Downstream 100 bases:

>100_bases
GGGCTCAGCCCAGGTCGGTCAGCGCCTGACGCGCGGCGTTGTAGCCGGGGATGAACGTGATGCCCGGCCCGCCGTGGCAT
CCGGCACTGGCCAGGTAGAG

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: TGS2; Diacylglycerol O-acyltransferase; DGAT; Long-chain-alcohol O-fatty-acyltransferase; Probable triacylglycerol synthase tgs2; Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase; Wax synthase; WS [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKLISPTDSMFLLVESREHPMHVGGLQLFEPPDGISGADFLREIHQAMVEQTDFQETFRKHPGTVLGGVANLAWAVDEDV
DLDYHLRRSALPSPGRVRELLELTSRLHGTLLDRHRPLWEAHLVEGLSDGRFAVYVKFHHALIDGVSALKLTQRTLSTDP
DDPEVRVPWNLPPKRRTREPESKSLLRSVTDRVGSVAALAPSTFGLARAALLEQQLQLPFGAPKTMFNVPIGGARRVAAQ
SWPLERIRSVKRATGATVNDVILAMCAGALRFYLTEQNALPDAPLIAMVPVSLRTEAEADSGGNMTGVVLCNLATDDDDP
ARRLDTISLSMRRNKQVFSQLPRVQQLALSASMIAPLGLGAVPGFVSTAPPPFNIVISNVPGNQEPMYWRGARLDGNYPF
SIALDGQALNITMANNGDNLDFGLVGARGSVPHLQRLLGHLEDSLKELEVAVGV

Sequences:

>Translated_454_residues
MKLISPTDSMFLLVESREHPMHVGGLQLFEPPDGISGADFLREIHQAMVEQTDFQETFRKHPGTVLGGVANLAWAVDEDV
DLDYHLRRSALPSPGRVRELLELTSRLHGTLLDRHRPLWEAHLVEGLSDGRFAVYVKFHHALIDGVSALKLTQRTLSTDP
DDPEVRVPWNLPPKRRTREPESKSLLRSVTDRVGSVAALAPSTFGLARAALLEQQLQLPFGAPKTMFNVPIGGARRVAAQ
SWPLERIRSVKRATGATVNDVILAMCAGALRFYLTEQNALPDAPLIAMVPVSLRTEAEADSGGNMTGVVLCNLATDDDDP
ARRLDTISLSMRRNKQVFSQLPRVQQLALSASMIAPLGLGAVPGFVSTAPPPFNIVISNVPGNQEPMYWRGARLDGNYPF
SIALDGQALNITMANNGDNLDFGLVGARGSVPHLQRLLGHLEDSLKELEVAVGV
>Mature_454_residues
MKLISPTDSMFLLVESREHPMHVGGLQLFEPPDGISGADFLREIHQAMVEQTDFQETFRKHPGTVLGGVANLAWAVDEDV
DLDYHLRRSALPSPGRVRELLELTSRLHGTLLDRHRPLWEAHLVEGLSDGRFAVYVKFHHALIDGVSALKLTQRTLSTDP
DDPEVRVPWNLPPKRRTREPESKSLLRSVTDRVGSVAALAPSTFGLARAALLEQQLQLPFGAPKTMFNVPIGGARRVAAQ
SWPLERIRSVKRATGATVNDVILAMCAGALRFYLTEQNALPDAPLIAMVPVSLRTEAEADSGGNMTGVVLCNLATDDDDP
ARRLDTISLSMRRNKQVFSQLPRVQQLALSASMIAPLGLGAVPGFVSTAPPPFNIVISNVPGNQEPMYWRGARLDGNYPF
SIALDGQALNITMANNGDNLDFGLVGARGSVPHLQRLLGHLEDSLKELEVAVGV

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Also functions as a wax synthase, incorporating palmityl alcohol into wax esters in the presence of palmitoyl-CoA [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20; =2.3.1.75 [H]

Molecular weight: Translated: 49540; Mature: 49540

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISPTDSMFLLVESREHPMHVGGLQLFEPPDGISGADFLREIHQAMVEQTDFQETFRK
CCCCCCCCCEEEEEECCCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
HPGTVLGGVANLAWAVDEDVDLDYHLRRSALPSPGRVRELLELTSRLHGTLLDRHRPLWE
CCCCCHHHHHHHHEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
AHLVEGLSDGRFAVYVKFHHALIDGVSALKLTQRTLSTDPDDPEVRVPWNLPPKRRTREP
HHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCC
ESKSLLRSVTDRVGSVAALAPSTFGLARAALLEQQLQLPFGAPKTMFNVPIGGARRVAAQ
HHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHC
SWPLERIRSVKRATGATVNDVILAMCAGALRFYLTEQNALPDAPLIAMVPVSLRTEAEAD
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEECCCCCCCCCCC
SGGNMTGVVLCNLATDDDDPARRLDTISLSMRRNKQVFSQLPRVQQLALSASMIAPLGLG
CCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
AVPGFVSTAPPPFNIVISNVPGNQEPMYWRGARLDGNYPFSIALDGQALNITMANNGDNL
CCCCCCCCCCCCCEEEEECCCCCCCCCEEECCEECCCCCEEEEECCCEEEEEEECCCCCC
DFGLVGARGSVPHLQRLLGHLEDSLKELEVAVGV
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLISPTDSMFLLVESREHPMHVGGLQLFEPPDGISGADFLREIHQAMVEQTDFQETFRK
CCCCCCCCCEEEEEECCCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
HPGTVLGGVANLAWAVDEDVDLDYHLRRSALPSPGRVRELLELTSRLHGTLLDRHRPLWE
CCCCCHHHHHHHHEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
AHLVEGLSDGRFAVYVKFHHALIDGVSALKLTQRTLSTDPDDPEVRVPWNLPPKRRTREP
HHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCC
ESKSLLRSVTDRVGSVAALAPSTFGLARAALLEQQLQLPFGAPKTMFNVPIGGARRVAAQ
HHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHC
SWPLERIRSVKRATGATVNDVILAMCAGALRFYLTEQNALPDAPLIAMVPVSLRTEAEAD
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEECCCCCCCCCCC
SGGNMTGVVLCNLATDDDDPARRLDTISLSMRRNKQVFSQLPRVQQLALSASMIAPLGLG
CCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
AVPGFVSTAPPPFNIVISNVPGNQEPMYWRGARLDGNYPFSIALDGQALNITMANNGDNL
CCCCCCCCCCCCCEEEEECCCCCCCCCEEECCEECCCCCEEEEECCCEEEEEEECCCCCC
DFGLVGARGSVPHLQRLLGHLEDSLKELEVAVGV
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]