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The map label for this gene is citA [H]

Identifier: 119870575

GI number: 119870575

Start: 4780371

End: 4781501

Strand: Direct

Name: citA [H]

Synonym: Mkms_4546

Alternate gene names: 119870575

Gene position: 4780371-4781501 (Clockwise)

Preceding gene: 119870569

Following gene: 119870576

Centisome position: 83.32

GC content: 71.09

Gene sequence:

>1131_bases
ATGACTCAGACGTCGCCGTCGGTGCCGGAGAATTTCGTCGAGGGACTCGAGGGTGTGGTGGCCTTCACCACCCAGATCGC
CGAACCGGACAAGGACGGCGGGGCGCTGCGGTACCGCGGCGTGGACATCGAGGATCTGGTCGCGCGGCGGGTGACGTTCG
GCGACGTGTGGGCACTGCTGGTCGACGGGCGCTTCGGCGACGGGCTACCGCCGGCCGAACCGTTCCCGCTGCCGATCCAC
AGCGGCGACGTCCGCGTCGACGTACAGGCCGGCCTCGCGATGCTCGCGCCGATCTGGGGCTACAAACCGATCCTCGACAT
CGACGACGACACCGCCCGCGCCCAACTCGCGCGCGCCTCGGTGATGGCCCTGTCCTACGTCGCGCAGTCCGCGCGCGGGA
TCCACCGGCCGGCCGTCCCACAGCGGTCGATCGACGAATGCACAACCGTGACAGCACGTTTCATGACGCGGTGGCAGGGT
GACCCCGATCCGAGGCACGTCGAGGCCATCGACGCCTACTGGGTCACCGCCGCCGAACACGGTATGAACGCCTCGACGTT
CACCGCCCGGGTGATCGCCTCCACCGGCGCCGACGTCGCGGCGGCACTCTCCGGCGCCGTCGGCGCGATGAGCGGCCCCC
TGCACGGCGGCGCACCCGCGCGGGTACTGCCGATGCTCGACGAGGTCGAGCGCACCGGTGACGCCCGCGCCGTGGTCAAG
GGCATCCTCGACCGCAAGGACAAGCTGATGGGGTTCGGACACCGGGTGTACCGGGCGGAGGATCCGCGGGCCCGTGTGCT
GCGCGCGACCGCCGAGCGCCTGGAGGCGCCGCGCTTCGAGGTCGCCGCCGCCCTCGAGCAGGCCGCACTGGCCGAGCTGC
GTGAGCGCCGTCCCGACCGGGCGATCGAGACGAACGTCGAGTTCTGGGCCGCCGTGATCCTCGACTTCGCCCAGGTGCCG
GCCTCGATGATGCCGGCGATGTTCACCTGTGGGCGCACCGCCGGGTGGTGCGCCCACATCCTCGAACAGAAACGACTGGG
CAAGCTGGTGCGCCCGTCGGCGATCTATGTCGGACCCGATCCGCGGAGCCCGGAGTCCGTCGAGGGCTGGGACCGCATCA
CCACCTCGTGA

Upstream 100 bases:

>100_bases
CGCGCATCCTCGCCAAGTGCTCGTTGTCGGCCTTTTTCACCGCGCCAACGCTACGCCGACCGGTCCGTCCGGCACTTGCT
GTGGGTGCGAGAATCTGGCC

Downstream 100 bases:

>100_bases
CCACCGATCCGGCGGTCGTGTTCGCCTCTGCCGCACGCAGTTTCGCCGATCTGGTGGGCCGGCTGCCGGCCACCGGGTGG
GACGGTCCCGGGTTGGGTGA

Product: citrate synthase 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTQTSPSVPENFVEGLEGVVAFTTQIAEPDKDGGALRYRGVDIEDLVARRVTFGDVWALLVDGRFGDGLPPAEPFPLPIH
SGDVRVDVQAGLAMLAPIWGYKPILDIDDDTARAQLARASVMALSYVAQSARGIHRPAVPQRSIDECTTVTARFMTRWQG
DPDPRHVEAIDAYWVTAAEHGMNASTFTARVIASTGADVAAALSGAVGAMSGPLHGGAPARVLPMLDEVERTGDARAVVK
GILDRKDKLMGFGHRVYRAEDPRARVLRATAERLEAPRFEVAAALEQAALAELRERRPDRAIETNVEFWAAVILDFAQVP
ASMMPAMFTCGRTAGWCAHILEQKRLGKLVRPSAIYVGPDPRSPESVEGWDRITTS

Sequences:

>Translated_376_residues
MTQTSPSVPENFVEGLEGVVAFTTQIAEPDKDGGALRYRGVDIEDLVARRVTFGDVWALLVDGRFGDGLPPAEPFPLPIH
SGDVRVDVQAGLAMLAPIWGYKPILDIDDDTARAQLARASVMALSYVAQSARGIHRPAVPQRSIDECTTVTARFMTRWQG
DPDPRHVEAIDAYWVTAAEHGMNASTFTARVIASTGADVAAALSGAVGAMSGPLHGGAPARVLPMLDEVERTGDARAVVK
GILDRKDKLMGFGHRVYRAEDPRARVLRATAERLEAPRFEVAAALEQAALAELRERRPDRAIETNVEFWAAVILDFAQVP
ASMMPAMFTCGRTAGWCAHILEQKRLGKLVRPSAIYVGPDPRSPESVEGWDRITTS
>Mature_375_residues
TQTSPSVPENFVEGLEGVVAFTTQIAEPDKDGGALRYRGVDIEDLVARRVTFGDVWALLVDGRFGDGLPPAEPFPLPIHS
GDVRVDVQAGLAMLAPIWGYKPILDIDDDTARAQLARASVMALSYVAQSARGIHRPAVPQRSIDECTTVTARFMTRWQGD
PDPRHVEAIDAYWVTAAEHGMNASTFTARVIASTGADVAAALSGAVGAMSGPLHGGAPARVLPMLDEVERTGDARAVVKG
ILDRKDKLMGFGHRVYRAEDPRARVLRATAERLEAPRFEVAAALEQAALAELRERRPDRAIETNVEFWAAVILDFAQVPA
SMMPAMFTCGRTAGWCAHILEQKRLGKLVRPSAIYVGPDPRSPESVEGWDRITTS

Specific function: Catalyzes The Synthesis Of 2-Methylcitrate From Propionyl-CoA And Oxaloacetate. Also Catalyzes The Condensation Of Oxaloacetate With Acetyl-CoA But With A Lower Specificity. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=374, Percent_Identity=32.8877005347594, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786939, Length=207, Percent_Identity=35.7487922705314, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 40743; Mature: 40612

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTSPSVPENFVEGLEGVVAFTTQIAEPDKDGGALRYRGVDIEDLVARRVTFGDVWALL
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHEEEHE
VDGRFGDGLPPAEPFPLPIHSGDVRVDVQAGLAMLAPIWGYKPILDIDDDTARAQLARAS
ECCCCCCCCCCCCCCCCCCCCCCEEEEHHHCHHHHHHHCCCCCEEECCCCHHHHHHHHHH
VMALSYVAQSARGIHRPAVPQRSIDECTTVTARFMTRWQGDPDPRHVEAIDAYWVTAAEH
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEHHHHC
GMNASTFTARVIASTGADVAAALSGAVGAMSGPLHGGAPARVLPMLDEVERTGDARAVVK
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
GILDRKDKLMGFGHRVYRAEDPRARVLRATAERLEAPRFEVAAALEQAALAELRERRPDR
HHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
AIETNVEFWAAVILDFAQVPASMMPAMFTCGRTAGWCAHILEQKRLGKLVRPSAIYVGPD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCC
PRSPESVEGWDRITTS
CCCCCCCCCCHHCCCC
>Mature Secondary Structure 
TQTSPSVPENFVEGLEGVVAFTTQIAEPDKDGGALRYRGVDIEDLVARRVTFGDVWALL
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHEEEHE
VDGRFGDGLPPAEPFPLPIHSGDVRVDVQAGLAMLAPIWGYKPILDIDDDTARAQLARAS
ECCCCCCCCCCCCCCCCCCCCCCEEEEHHHCHHHHHHHCCCCCEEECCCCHHHHHHHHHH
VMALSYVAQSARGIHRPAVPQRSIDECTTVTARFMTRWQGDPDPRHVEAIDAYWVTAAEH
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEHHHHC
GMNASTFTARVIASTGADVAAALSGAVGAMSGPLHGGAPARVLPMLDEVERTGDARAVVK
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
GILDRKDKLMGFGHRVYRAEDPRARVLRATAERLEAPRFEVAAALEQAALAELRERRPDR
HHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
AIETNVEFWAAVILDFAQVPASMMPAMFTCGRTAGWCAHILEQKRLGKLVRPSAIYVGPD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCC
PRSPESVEGWDRITTS
CCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]