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The map label for this gene is rnhA [H]

Identifier: 119870465

GI number: 119870465

Start: 4667440

End: 4667904

Strand: Direct

Name: rnhA [H]

Synonym: Mkms_4436

Alternate gene names: 119870465

Gene position: 4667440-4667904 (Clockwise)

Preceding gene: 119870464

Following gene: 119870468

Centisome position: 81.35

GC content: 69.25

Gene sequence:

>465_bases
GTGGACACCGAACCCGTGATCATCCACACCGACGGCGGCTGCCGCCCCAACCCGGGTCCCGGCGGCTGGGGCGCGGTGCT
GCGTCACCGCAACCACATCCGCGAGATGTGTGGCGGCGAACCCGGGGCGACGAGCAACAACCGGATGGAACTGACCGCGC
CGATCATGGCGCTCGAGGCGCTCACCAGACCGGTCGTGGTGCACCTGCACACCGACAGCACCTATGTCCGCAACGGCATC
ACCAAATGGGTGCTGGGCTGGGAGCGCAACGGCTGGCTGACCGCCGCCAAACAGCCGGTGAAGAACGTCGACCTGTGGCA
GCGGCTGCAGGCCGCCTGCGCGCGCCACCGTGTCGAGTGGTTCTGGGTGAAGGGCCACTCGGGTGTCGCGGACAACGAGT
TGGCCGACCAGTTGGCCACCCGCGGCCTGCGGCAGGCGCTCGAAGCCGCCGCGATTGTCCTCTGA

Upstream 100 bases:

>100_bases
CGCGCTGATCTTCCCGCGAATCTCGAGGAACTGCTGGCCCTGCACGGTGTCGGGCCCAAGGCAGTCGAGCTGCTCCGTCG
GGCAGAGGCCGGCTGAGCCC

Downstream 100 bases:

>100_bases
CCGGCCGTATGCGCTACCAGCCTGCAGGCGTTTGGCTTTCGCGCCGCGGCGGCAGTCCCAGCGGTGAGGTGTGGCCATGG
ACGTCCGTCAGCGCACCGAC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MDTEPVIIHTDGGCRPNPGPGGWGAVLRHRNHIREMCGGEPGATSNNRMELTAPIMALEALTRPVVVHLHTDSTYVRNGI
TKWVLGWERNGWLTAAKQPVKNVDLWQRLQAACARHRVEWFWVKGHSGVADNELADQLATRGLRQALEAAAIVL

Sequences:

>Translated_154_residues
MDTEPVIIHTDGGCRPNPGPGGWGAVLRHRNHIREMCGGEPGATSNNRMELTAPIMALEALTRPVVVHLHTDSTYVRNGI
TKWVLGWERNGWLTAAKQPVKNVDLWQRLQAACARHRVEWFWVKGHSGVADNELADQLATRGLRQALEAAAIVL
>Mature_154_residues
MDTEPVIIHTDGGCRPNPGPGGWGAVLRHRNHIREMCGGEPGATSNNRMELTAPIMALEALTRPVVVHLHTDSTYVRNGI
TKWVLGWERNGWLTAAKQPVKNVDLWQRLQAACARHRVEWFWVKGHSGVADNELADQLATRGLRQALEAAAIVL

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=150, Percent_Identity=37.3333333333333, Blast_Score=81, Evalue=4e-16,
Organism=Escherichia coli, GI1786408, Length=136, Percent_Identity=55.8823529411765, Blast_Score=153, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI115532542, Length=148, Percent_Identity=32.4324324324324, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45552509, Length=144, Percent_Identity=35.4166666666667, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17137476, Length=144, Percent_Identity=35.4166666666667, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 17041; Mature: 17041

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTEPVIIHTDGGCRPNPGPGGWGAVLRHRNHIREMCGGEPGATSNNRMELTAPIMALEA
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHH
LTRPVVVHLHTDSTYVRNGITKWVLGWERNGWLTAAKQPVKNVDLWQRLQAACARHRVEW
HCCCEEEEEECCCHHHHCCHHHHHEEECCCCEEECCCCCCCCHHHHHHHHHHHHHCCCEE
FWVKGHSGVADNELADQLATRGLRQALEAAAIVL
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDTEPVIIHTDGGCRPNPGPGGWGAVLRHRNHIREMCGGEPGATSNNRMELTAPIMALEA
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHH
LTRPVVVHLHTDSTYVRNGITKWVLGWERNGWLTAAKQPVKNVDLWQRLQAACARHRVEW
HCCCEEEEEECCCHHHHCCHHHHHEEECCCCEEECCCCCCCCHHHHHHHHHHHHHCCCEE
FWVKGHSGVADNELADQLATRGLRQALEAAAIVL
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA