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The map label for this gene is pinE [C]

Identifier: 119870328

GI number: 119870328

Start: 4524356

End: 4525765

Strand: Direct

Name: pinE [C]

Synonym: Mkms_4299

Alternate gene names: 119870328

Gene position: 4524356-4525765 (Clockwise)

Preceding gene: 119870327

Following gene: 119870329

Centisome position: 78.86

GC content: 70.21

Gene sequence:

>1410_bases
ATGAGAGCAGCCATCTACACCCGCATCAGCAAGGACCAGACCGGCGAGGGCCTGGGCGTTCAGCGCCAACTGGACGCCTG
CCTGGCGCTCGCCGACCGGCTGGGTTGGGCCGTCGTCACCCGCTACGACGACAACGACCTCAGCGCCTACAACGGCAAGA
CCCGCCCCGGCTTCGAGGCACTGCTCGACGGCATGAAGCGCGGCGAGTTCGACGCCGTCATCTGCTGGCACACCGACCGC
CTGTATCGCAGCCTCAAGGACTTGGTGCGGCTGCTGGAGGTGGCCGTCGGCATCGACATCCGCACTGTGTGCGGCGGCGA
CCTCGACCTGTCCACGCCGACCGGGCGCATGCTGGCGACCATCCTGGGCGGTGTGGCCGTGCAGGAGTCCGAGCACAAGG
GTGAGCGCCAGCGCGAGGCCAACGAGCAGCGCGCCGCAGCCGGGAGGTGGCACACCGCCAACCGCCCGTTCGGCTACACG
ATGAGCGGGGAGGCGCTGGAGCCGGAAGCCTCAGCGGTGCGCCAAGCCGCCGCCGACGTGCTCGCCGGGAAGAGCATCCG
GCGGGTGGCGATGGAGTGGAACGAGGCCGGGTTGCGGTCGACGCTGGCGGGCCGCGAGCGCAAGAGCAAGGACGGCACGG
TGGTGAAGGTGGTCGACGGCCGGTGGAACGCGCCACGGGTGCGTCAACTGCTGCTCAGGCCGCGCTACGCGGGCCTGCGG
GTGTACCGGGGCAAGGTCGTCGGCAAGGGCGACTGGGAACCGCTGATCGATGAGGACACCCACCGTGGCCTGGTCGCGTT
CCTGAGCGACCCGTCGCGAGCGCCGCGCACCAAGTTCGAGCGCAAGTACCAGGGTGCCGGGGTGTACCGGTGCGGCAAGT
GCGGCGGCCCGATGCGGACGGGCAAGCCCAAGGGTGGACGGCGAACCTACCTGTGCCGGGAGTACTCCCACGTCGTGCGC
CAGGGCGAGTCGCTGGACCGCTACGTCGAGATGCTGGTGCTCGACCGGCTCTCGCGGCCCGACGTTCACCTGGTGCTCGC
TGGCGGTGACCGGCTCGACCTGCCGACGCTGCACGCTCGCCGAGAAGCGTTGCAGGCCCGCCTCGATGAGGCCGCAGGCA
TGTTCGCCAAACGCGAGATCGACGGCGCGCAGCTACGCCGGATCACCAACGACCTGCGCACCGAGGTCGCGGGCGTCGAC
AAGGTGCTGGCCGACGCCGCCCGCGTCAGCCCGGTCACCGGCCTGATCAGTGAGAGCACCGACCGCGACGAGGTGCAGCA
GCGGTGGGAGGCGCTGTCGCCGGACCTGCGCGGCAAGGTGATCGATGAGTTGATGACGGTGACCGTGCAGCCCGCGCCTC
GCGGCACCAACGGCTTCCATCCCGAGCACATCAGCGTCGAGTGGAAGTAG

Upstream 100 bases:

>100_bases
ATCCTCTACCTCGGCCGCGCCAAACGCTGCGCCACCACCGCCCAACGCCTAGCCCTGTTCGCCCGAGAAAAGTGGACTCG
GATACTGATACCTTGGCGGC

Downstream 100 bases:

>100_bases
CGCCACCGGCGAAGTAGCTCCTACGTCAGCCCCTGTCGGACCACACCGGCGGGGGCTGACTCTATTGACGGGGTCAACCT
ACGAGTGAGCGCGTCACGAA

Product: recombinase

Products: NA

Alternate protein names: Resolvase Domain Protein; Resolvase Domain-Containing Protein; Site-Specific Recombinase DNA Invertase Pin; Site-Specific Recombinase; Integrase/Recombinase; Integrase; Resolvase; Site-Specific DNA Recombinase; Site-Specific Integrase; Site-Specific Recombinase For Integration And Excision; Cassette Chromosome Recombinase B; Resolvase Family Site-Specific Recombinase; Site-Specific Recombinase Resolvase Family; DNA Recombinase; Ntegrase/Recombinase.; Resolvase Domain; Transposase; Site-Specific Recombinase Resolvase Domain Protein; Phage Integrase; Resolvase-Like Protein; Phague Integrase; Site-Specific Recombinase/Resolvase; Integrase /Recombinase; Bacteriophage; PhiRv1 Phage Integrase; Site-Specific Integrase Protein

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MRAAIYTRISKDQTGEGLGVQRQLDACLALADRLGWAVVTRYDDNDLSAYNGKTRPGFEALLDGMKRGEFDAVICWHTDR
LYRSLKDLVRLLEVAVGIDIRTVCGGDLDLSTPTGRMLATILGGVAVQESEHKGERQREANEQRAAAGRWHTANRPFGYT
MSGEALEPEASAVRQAAADVLAGKSIRRVAMEWNEAGLRSTLAGRERKSKDGTVVKVVDGRWNAPRVRQLLLRPRYAGLR
VYRGKVVGKGDWEPLIDEDTHRGLVAFLSDPSRAPRTKFERKYQGAGVYRCGKCGGPMRTGKPKGGRRTYLCREYSHVVR
QGESLDRYVEMLVLDRLSRPDVHLVLAGGDRLDLPTLHARREALQARLDEAAGMFAKREIDGAQLRRITNDLRTEVAGVD
KVLADAARVSPVTGLISESTDRDEVQQRWEALSPDLRGKVIDELMTVTVQPAPRGTNGFHPEHISVEWK

Sequences:

>Translated_469_residues
MRAAIYTRISKDQTGEGLGVQRQLDACLALADRLGWAVVTRYDDNDLSAYNGKTRPGFEALLDGMKRGEFDAVICWHTDR
LYRSLKDLVRLLEVAVGIDIRTVCGGDLDLSTPTGRMLATILGGVAVQESEHKGERQREANEQRAAAGRWHTANRPFGYT
MSGEALEPEASAVRQAAADVLAGKSIRRVAMEWNEAGLRSTLAGRERKSKDGTVVKVVDGRWNAPRVRQLLLRPRYAGLR
VYRGKVVGKGDWEPLIDEDTHRGLVAFLSDPSRAPRTKFERKYQGAGVYRCGKCGGPMRTGKPKGGRRTYLCREYSHVVR
QGESLDRYVEMLVLDRLSRPDVHLVLAGGDRLDLPTLHARREALQARLDEAAGMFAKREIDGAQLRRITNDLRTEVAGVD
KVLADAARVSPVTGLISESTDRDEVQQRWEALSPDLRGKVIDELMTVTVQPAPRGTNGFHPEHISVEWK
>Mature_469_residues
MRAAIYTRISKDQTGEGLGVQRQLDACLALADRLGWAVVTRYDDNDLSAYNGKTRPGFEALLDGMKRGEFDAVICWHTDR
LYRSLKDLVRLLEVAVGIDIRTVCGGDLDLSTPTGRMLATILGGVAVQESEHKGERQREANEQRAAAGRWHTANRPFGYT
MSGEALEPEASAVRQAAADVLAGKSIRRVAMEWNEAGLRSTLAGRERKSKDGTVVKVVDGRWNAPRVRQLLLRPRYAGLR
VYRGKVVGKGDWEPLIDEDTHRGLVAFLSDPSRAPRTKFERKYQGAGVYRCGKCGGPMRTGKPKGGRRTYLCREYSHVVR
QGESLDRYVEMLVLDRLSRPDVHLVLAGGDRLDLPTLHARREALQARLDEAAGMFAKREIDGAQLRRITNDLRTEVAGVD
KVLADAARVSPVTGLISESTDRDEVQQRWEALSPDLRGKVIDELMTVTVQPAPRGTNGFHPEHISVEWK

Specific function: This Protein Catalyzes The Inversion Of An 1800-Bp E.Coli DNA Fragment, The P Region, Which Can Exist In Either Orientation. The Function Of The Inversion Is Not Yet Clear. [C]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 52158; Mature: 52158

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAAIYTRISKDQTGEGLGVQRQLDACLALADRLGWAVVTRYDDNDLSAYNGKTRPGFEA
CCCHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHH
LLDGMKRGEFDAVICWHTDRLYRSLKDLVRLLEVAVGIDIRTVCGGDLDLSTPTGRMLAT
HHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHH
ILGGVAVQESEHKGERQREANEQRAAAGRWHTANRPFGYTMSGEALEPEASAVRQAAADV
HHCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LAGKSIRRVAMEWNEAGLRSTLAGRERKSKDGTVVKVVDGRWNAPRVRQLLLRPRYAGLR
HHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEE
VYRGKVVGKGDWEPLIDEDTHRGLVAFLSDPSRAPRTKFERKYQGAGVYRCGKCGGPMRT
EEECEEECCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEECCCCCCCCC
GKPKGGRRTYLCREYSHVVRQGESLDRYVEMLVLDRLSRPDVHLVLAGGDRLDLPTLHAR
CCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
REALQARLDEAAGMFAKREIDGAQLRRITNDLRTEVAGVDKVLADAARVSPVTGLISEST
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCC
DRDEVQQRWEALSPDLRGKVIDELMTVTVQPAPRGTNGFHPEHISVEWK
CHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MRAAIYTRISKDQTGEGLGVQRQLDACLALADRLGWAVVTRYDDNDLSAYNGKTRPGFEA
CCCHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHH
LLDGMKRGEFDAVICWHTDRLYRSLKDLVRLLEVAVGIDIRTVCGGDLDLSTPTGRMLAT
HHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHH
ILGGVAVQESEHKGERQREANEQRAAAGRWHTANRPFGYTMSGEALEPEASAVRQAAADV
HHCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LAGKSIRRVAMEWNEAGLRSTLAGRERKSKDGTVVKVVDGRWNAPRVRQLLLRPRYAGLR
HHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEE
VYRGKVVGKGDWEPLIDEDTHRGLVAFLSDPSRAPRTKFERKYQGAGVYRCGKCGGPMRT
EEECEEECCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEECCCCCCCCC
GKPKGGRRTYLCREYSHVVRQGESLDRYVEMLVLDRLSRPDVHLVLAGGDRLDLPTLHAR
CCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
REALQARLDEAAGMFAKREIDGAQLRRITNDLRTEVAGVDKVLADAARVSPVTGLISEST
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCC
DRDEVQQRWEALSPDLRGKVIDELMTVTVQPAPRGTNGFHPEHISVEWK
CHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA