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The map label for this gene is paaJ [C]

Identifier: 119870270

GI number: 119870270

Start: 4466244

End: 4467437

Strand: Direct

Name: paaJ [C]

Synonym: Mkms_4240

Alternate gene names: 119870270

Gene position: 4466244-4467437 (Clockwise)

Preceding gene: 119870268

Following gene: 119870271

Centisome position: 77.85

GC content: 66.67

Gene sequence:

>1194_bases
ATGAGGGGAAACCGCGTGTTCGTCGTCGGAGTCGGGATGACGAAGTTCGAGAAGCCGGGCTCTCGTGAAGGCTGGGACTA
CCCGCAGATGGCGAAGGAGTCGGGGACCAACGCGCTGGCCGACGCCGGCATCGAGTACTCGGCGGTCGAGCACGGCTTCG
TCGGGTACTGCTCGGGCGACTCGACCTCGGGTCAGCGCGCGCTCTACGAACTCGGCATGACCGGCATCCCGGTGGTCAAC
GTCAACAACAACTGCTCGACGGGTTCGACGGCGCTGTATCTGGCCGCCCAGACGATCCGCGGCGGGTTGGCCGACTGCAC
CATCGCGCTGGGCTTCGAGAAGATGCAGCCCGGATCGCTGAGCGGCGGGGCGAGCGACCGGGAGTCGCCGATGGGTCGCC
ACATCAAGGCGATGGCCGAGATCGACGAGTTCGCGATGCCGGTGGCGCCGTGGATGTTCGGCGCGGCCGGCCGAGAGCAC
ATGCGCCGGCACGGTTCCACCGCCGAGCATTTTGCGAAGATCGGCTACAAGAACCACAAGCATTCGGTCAACAACCCCTA
CGCCCAGTTCCAGGAGGAGTACACCCTCGACGACATCCTCGGCTCCCGGATGATCTCCGACCCGCTGACCAAGCTGCAGT
GCTCACCCACCTCCGACGGTTCGGGTGCGGCGATCCTGGCCAGTGAGGCGTTCGTCGACGAGCACGGGCTGGCCGACCGC
GCCGTCGAGATCGTCGGCCAGGCGATGACCACCGACTTCGAGAGCACCTTCGACGGCACCGCCAAGAACCTGATCGGCTA
CGACATGAATGTCAAAGCGGCCCAACAGGTCTACGCGCAGTCCGGACTGGGTCCCGCCGACTTCCAGGTGATCGAGCTGC
ACGACTGCTTCTCGGCCAACGAACTGTTGCTGTACGAGGCGCTGGGCCTGTGCGGTGAGGGTGAGGCGCCGAAGCTGATC
GACAACGACGACACCACCTACGGCGGACGGTGGGTGGTCAACCCGTCGGGCGGACTGATCTCCAAGGGCCATCCGCTGGG
TGCGACGGGCCTGGCGCAGTGCGCCGAGCTGACGTGGCAGCTGCGGGGAACCGCCGACGCGCGTCAGGTCGACAACGTCA
CCGCTGCGCTGCAGCACAACATCGGGTTGGGTGGCGCGGCGGTGGTCACGGCCTATCAGCGCGCCGACCGCTGA

Upstream 100 bases:

>100_bases
CACTGCACCACCCGTAACGCCACGACGCCTCCTTCACGGCTCACTAGAACACGTTCTATTGTGGTCTACACCACATCGGG
CCGTAAGCGAAGGGAACGCC

Downstream 100 bases:

>100_bases
AACCGTCGTTGAACCGTTCCGGTCGCCACGTCGTTTCCACCACAGATCTCTGGTACGCGGGAAGTTTTCCCATATCGCCC
GGGGTTCTAGCGGACGACAA

Product: lipid-transfer protein

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MRGNRVFVVGVGMTKFEKPGSREGWDYPQMAKESGTNALADAGIEYSAVEHGFVGYCSGDSTSGQRALYELGMTGIPVVN
VNNNCSTGSTALYLAAQTIRGGLADCTIALGFEKMQPGSLSGGASDRESPMGRHIKAMAEIDEFAMPVAPWMFGAAGREH
MRRHGSTAEHFAKIGYKNHKHSVNNPYAQFQEEYTLDDILGSRMISDPLTKLQCSPTSDGSGAAILASEAFVDEHGLADR
AVEIVGQAMTTDFESTFDGTAKNLIGYDMNVKAAQQVYAQSGLGPADFQVIELHDCFSANELLLYEALGLCGEGEAPKLI
DNDDTTYGGRWVVNPSGGLISKGHPLGATGLAQCAELTWQLRGTADARQVDNVTAALQHNIGLGGAAVVTAYQRADR

Sequences:

>Translated_397_residues
MRGNRVFVVGVGMTKFEKPGSREGWDYPQMAKESGTNALADAGIEYSAVEHGFVGYCSGDSTSGQRALYELGMTGIPVVN
VNNNCSTGSTALYLAAQTIRGGLADCTIALGFEKMQPGSLSGGASDRESPMGRHIKAMAEIDEFAMPVAPWMFGAAGREH
MRRHGSTAEHFAKIGYKNHKHSVNNPYAQFQEEYTLDDILGSRMISDPLTKLQCSPTSDGSGAAILASEAFVDEHGLADR
AVEIVGQAMTTDFESTFDGTAKNLIGYDMNVKAAQQVYAQSGLGPADFQVIELHDCFSANELLLYEALGLCGEGEAPKLI
DNDDTTYGGRWVVNPSGGLISKGHPLGATGLAQCAELTWQLRGTADARQVDNVTAALQHNIGLGGAAVVTAYQRADR
>Mature_397_residues
MRGNRVFVVGVGMTKFEKPGSREGWDYPQMAKESGTNALADAGIEYSAVEHGFVGYCSGDSTSGQRALYELGMTGIPVVN
VNNNCSTGSTALYLAAQTIRGGLADCTIALGFEKMQPGSLSGGASDRESPMGRHIKAMAEIDEFAMPVAPWMFGAAGREH
MRRHGSTAEHFAKIGYKNHKHSVNNPYAQFQEEYTLDDILGSRMISDPLTKLQCSPTSDGSGAAILASEAFVDEHGLADR
AVEIVGQAMTTDFESTFDGTAKNLIGYDMNVKAAQQVYAQSGLGPADFQVIELHDCFSANELLLYEALGLCGEGEAPKLI
DNDDTTYGGRWVVNPSGGLISKGHPLGATGLAQCAELTWQLRGTADARQVDNVTAALQHNIGLGGAAVVTAYQRADR

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI19923233, Length=394, Percent_Identity=62.1827411167513, Blast_Score=494, Evalue=1e-140,
Organism=Homo sapiens, GI302344760, Length=394, Percent_Identity=58.6294416243655, Blast_Score=457, Evalue=1e-129,
Organism=Homo sapiens, GI302344767, Length=325, Percent_Identity=61.5384615384615, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI302344762, Length=393, Percent_Identity=54.4529262086514, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI55956775, Length=355, Percent_Identity=52.3943661971831, Blast_Score=348, Evalue=6e-96,
Organism=Escherichia coli, GI1787663, Length=307, Percent_Identity=27.6872964169381, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17537653, Length=382, Percent_Identity=51.8324607329843, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24585051, Length=396, Percent_Identity=63.6363636363636, Blast_Score=510, Evalue=1e-145,
Organism=Drosophila melanogaster, GI19921506, Length=396, Percent_Identity=62.8787878787879, Blast_Score=508, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42232; Mature: 42232

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGNRVFVVGVGMTKFEKPGSREGWDYPQMAKESGTNALADAGIEYSAVEHGFVGYCSGD
CCCCEEEEEECCHHHCCCCCCCCCCCCHHHHHHCCCCHHHHCCCEEEEECCCEEEEECCC
STSGQRALYELGMTGIPVVNVNNNCSTGSTALYLAAQTIRGGLADCTIALGFEKMQPGSL
CCCHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEHHHHCCCCCCEEEEECHHHCCCCCC
SGGASDRESPMGRHIKAMAEIDEFAMPVAPWMFGAAGREHMRRHGSTAEHFAKIGYKNHK
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCCCC
HSVNNPYAQFQEEYTLDDILGSRMISDPLTKLQCSPTSDGSGAAILASEAFVDEHGLADR
CCCCCCHHHHHHHCCHHHHHHHHHHHCCHHHEEECCCCCCCCCEEEEHHHHHHHCCCHHH
AVEIVGQAMTTDFESTFDGTAKNLIGYDMNVKAAQQVYAQSGLGPADFQVIELHDCFSAN
HHHHHHHHHHCCHHHHCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
ELLLYEALGLCGEGEAPKLIDNDDTTYGGRWVVNPSGGLISKGHPLGATGLAQCAELTWQ
HHHHHHHHCCCCCCCCCCEECCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHEE
LRGTADARQVDNVTAALQHNIGLGGAAVVTAYQRADR
ECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRGNRVFVVGVGMTKFEKPGSREGWDYPQMAKESGTNALADAGIEYSAVEHGFVGYCSGD
CCCCEEEEEECCHHHCCCCCCCCCCCCHHHHHHCCCCHHHHCCCEEEEECCCEEEEECCC
STSGQRALYELGMTGIPVVNVNNNCSTGSTALYLAAQTIRGGLADCTIALGFEKMQPGSL
CCCHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEHHHHCCCCCCEEEEECHHHCCCCCC
SGGASDRESPMGRHIKAMAEIDEFAMPVAPWMFGAAGREHMRRHGSTAEHFAKIGYKNHK
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCCCC
HSVNNPYAQFQEEYTLDDILGSRMISDPLTKLQCSPTSDGSGAAILASEAFVDEHGLADR
CCCCCCHHHHHHHCCHHHHHHHHHHHCCHHHEEECCCCCCCCCEEEEHHHHHHHCCCHHH
AVEIVGQAMTTDFESTFDGTAKNLIGYDMNVKAAQQVYAQSGLGPADFQVIELHDCFSAN
HHHHHHHHHHCCHHHHCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
ELLLYEALGLCGEGEAPKLIDNDDTTYGGRWVVNPSGGLISKGHPLGATGLAQCAELTWQ
HHHHHHHHCCCCCCCCCCEECCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHEE
LRGTADARQVDNVTAALQHNIGLGGAAVVTAYQRADR
ECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]