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The map label for this gene is glcD [H]

Identifier: 119870065

GI number: 119870065

Start: 4253918

End: 4255291

Strand: Direct

Name: glcD [H]

Synonym: Mkms_4035

Alternate gene names: 119870065

Gene position: 4253918-4255291 (Clockwise)

Preceding gene: 119870064

Following gene: 119870066

Centisome position: 74.15

GC content: 70.52

Gene sequence:

>1374_bases
ATGGCGGACGTGAACTCCGCGCTCGCCGAACTGATCGCCGACCTGTCCGACGGTGTCGTCGTCACCGACCCCGACATCGT
GGCGTCCTACCGGCAGGACCGCGCGGCCGATCCGAGCGCGGGAACCGCGCTGGCCGTCGTGCGCCCCACCCGCACCGAGG
AGGTGCAGACGGTGTTGCGGTGGGCGTCGGCGCACAAGGTGGCCGTGGTCCCACGCGGCATGGGCACCGGACTCTCCGGC
GGTGCGACCGCGCTCGACGGCGCGATCGTGCTGTCGACCGAGAAGATGCGTGACATCACCGTCGACCCGGTCACCCGCAC
CGCGGTGGCCCAGCCCGGCCTGCTCAACGCCGAGGTCAAGAAGGCGGTCGCCGAATACGGACTGTGGTATCCGCCCGACC
CGTCGTCGTACGAGATCTGCAGCATCGGCGGCAACGTCGCCACCAACGCCGGCGGGCTGTGCTGTGTGAAGTACGGCGTC
ACCACCGACTACGTGCTCGGCCTGCAGGTGGTCCTCGCCGACGGCACCGCGGTGCGCCTCGGTGGGCCGCGCCTCAAGGA
CGTGGCGGGGCTGTCGCTGACCAAGCTCTTCGTCGGCAGCGAGGGGACGCTCGGCGTGATCACCGAGGTGACGCTGCGCC
TGCTGCCGCCCCAGCCGTCGCCGTGCACGGTGGTCGCCACATTCGACTCCGTCGAGGCGGCGGCGGGCGCCGTGGTCACC
ATCACCGGCAGAATCCGGCCGTCGATGCTGGAGTTCATGGACGCGGTCGCGATCAACGCGGTCGAGGACAAGCTGCGGAT
GGGCCTGGACCGCAACGCCGCCGCCATGATGGTCGCCGCCTCCGACGACCGGGGCGCCGCCGGCGCCGAGGACGCCGAGT
TCATGGCGAAGGTGTTCACCGAAGCCGGTGCGACAGAGGTGTTCTCGACCTCGGATCCCGACGAGGGTGAGGCGTTCGTG
GCAGCCCGTCGATTCGCGATCCCGGCCGTCGAGGCCAAGGGCGCGCTGCTGCTCGAAGACGTCGGCGTGCCGGTCCCCCG
GCTCGCCGACCTGTGCGCAGGCATCGAGAAGATCGCCGCCGACCGTGACGTGATGATCTCGGTGATCGCTCATGCCGGCG
ACGGCAACACCCATCCACTGATCGTGTTCGACCCGGCCGACGCGGCGATGGCCGAACGTGCCGAGACGGCCTTCGGCGAG
ATCATGGACCTCGCCGTCGGCCTGGGCGGCACCATCACCGGCGAACACGGTGTCGGTCGGCTGAAGCGGCCGTGGCTGGC
CGGCCAGATCGGGCCGGAGGCGATGGAACTCAACCGTCGCATCAAACAGGCGCTCGACCCCGACGCGATCCTCAACCCGG
GCGCGGCGTTCTAG

Upstream 100 bases:

>100_bases
CACCGCCCTGCACACCGTGGCGATCAACGAGGTGTCGCTGCGCGCCGTGCACTACCGGGTCTTCGCGGTCCGCTGAGCAC
CGGTTGCGCACCGACCTAGC

Downstream 100 bases:

>100_bases
CCAAGCAGTAGAGACCGGACCACGGGCCGTGGTATCCATGAGACATGACGACGAATGTGTCTCATGCGGCCCGGTTCGAA
TTGGCGACGGCCGAGACGTG

Product: D-lactate dehydrogenase (cytochrome)

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MADVNSALAELIADLSDGVVVTDPDIVASYRQDRAADPSAGTALAVVRPTRTEEVQTVLRWASAHKVAVVPRGMGTGLSG
GATALDGAIVLSTEKMRDITVDPVTRTAVAQPGLLNAEVKKAVAEYGLWYPPDPSSYEICSIGGNVATNAGGLCCVKYGV
TTDYVLGLQVVLADGTAVRLGGPRLKDVAGLSLTKLFVGSEGTLGVITEVTLRLLPPQPSPCTVVATFDSVEAAAGAVVT
ITGRIRPSMLEFMDAVAINAVEDKLRMGLDRNAAAMMVAASDDRGAAGAEDAEFMAKVFTEAGATEVFSTSDPDEGEAFV
AARRFAIPAVEAKGALLLEDVGVPVPRLADLCAGIEKIAADRDVMISVIAHAGDGNTHPLIVFDPADAAMAERAETAFGE
IMDLAVGLGGTITGEHGVGRLKRPWLAGQIGPEAMELNRRIKQALDPDAILNPGAAF

Sequences:

>Translated_457_residues
MADVNSALAELIADLSDGVVVTDPDIVASYRQDRAADPSAGTALAVVRPTRTEEVQTVLRWASAHKVAVVPRGMGTGLSG
GATALDGAIVLSTEKMRDITVDPVTRTAVAQPGLLNAEVKKAVAEYGLWYPPDPSSYEICSIGGNVATNAGGLCCVKYGV
TTDYVLGLQVVLADGTAVRLGGPRLKDVAGLSLTKLFVGSEGTLGVITEVTLRLLPPQPSPCTVVATFDSVEAAAGAVVT
ITGRIRPSMLEFMDAVAINAVEDKLRMGLDRNAAAMMVAASDDRGAAGAEDAEFMAKVFTEAGATEVFSTSDPDEGEAFV
AARRFAIPAVEAKGALLLEDVGVPVPRLADLCAGIEKIAADRDVMISVIAHAGDGNTHPLIVFDPADAAMAERAETAFGE
IMDLAVGLGGTITGEHGVGRLKRPWLAGQIGPEAMELNRRIKQALDPDAILNPGAAF
>Mature_456_residues
ADVNSALAELIADLSDGVVVTDPDIVASYRQDRAADPSAGTALAVVRPTRTEEVQTVLRWASAHKVAVVPRGMGTGLSGG
ATALDGAIVLSTEKMRDITVDPVTRTAVAQPGLLNAEVKKAVAEYGLWYPPDPSSYEICSIGGNVATNAGGLCCVKYGVT
TDYVLGLQVVLADGTAVRLGGPRLKDVAGLSLTKLFVGSEGTLGVITEVTLRLLPPQPSPCTVVATFDSVEAAAGAVVTI
TGRIRPSMLEFMDAVAINAVEDKLRMGLDRNAAAMMVAASDDRGAAGAEDAEFMAKVFTEAGATEVFSTSDPDEGEAFVA
ARRFAIPAVEAKGALLLEDVGVPVPRLADLCAGIEKIAADRDVMISVIAHAGDGNTHPLIVFDPADAAMAERAETAFGEI
MDLAVGLGGTITGEHGVGRLKRPWLAGQIGPEAMELNRRIKQALDPDAILNPGAAF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI119964728, Length=455, Percent_Identity=32.0879120879121, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI37595756, Length=420, Percent_Identity=32.3809523809524, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI37595754, Length=443, Percent_Identity=30.9255079006772, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI4501993, Length=217, Percent_Identity=30.8755760368664, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789351, Length=414, Percent_Identity=41.5458937198068, Blast_Score=284, Evalue=6e-78,
Organism=Escherichia coli, GI1787977, Length=492, Percent_Identity=25, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI48994907, Length=178, Percent_Identity=35.9550561797753, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI48994914, Length=119, Percent_Identity=34.453781512605, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71992373, Length=466, Percent_Identity=31.1158798283262, Blast_Score=201, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI71992381, Length=466, Percent_Identity=29.6137339055794, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17534361, Length=417, Percent_Identity=29.2565947242206, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71992392, Length=380, Percent_Identity=31.3157894736842, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17556096, Length=461, Percent_Identity=23.4273318872017, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320023, Length=448, Percent_Identity=28.125, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6320027, Length=427, Percent_Identity=28.1030444964871, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320764, Length=449, Percent_Identity=25.8351893095768, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI18921117, Length=423, Percent_Identity=29.7872340425532, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24639277, Length=423, Percent_Identity=29.7872340425532, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24639275, Length=423, Percent_Identity=29.7872340425532, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24653753, Length=451, Percent_Identity=24.1685144124169, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 47284; Mature: 47153

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVNSALAELIADLSDGVVVTDPDIVASYRQDRAADPSAGTALAVVRPTRTEEVQTVLR
CCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHH
WASAHKVAVVPRGMGTGLSGGATALDGAIVLSTEKMRDITVDPVTRTAVAQPGLLNAEVK
HHCCCEEEEEECCCCCCCCCCCHHHCCEEEEECHHHCCCCCCCCHHHHHCCCCCCCHHHH
KAVAEYGLWYPPDPSSYEICSIGGNVATNAGGLCCVKYGVTTDYVLGLQVVLADGTAVRL
HHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHEEEEEEEEECCCEEEE
GGPRLKDVAGLSLTKLFVGSEGTLGVITEVTLRLLPPQPSPCTVVATFDSVEAAAGAVVT
CCCCHHHHCCCEEEEEEECCCCCEEHHEEEEHEECCCCCCCEEEEEECCCHHHHCCEEEE
ITGRIRPSMLEFMDAVAINAVEDKLRMGLDRNAAAMMVAASDDRGAAGAEDAEFMAKVFT
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
EAGATEVFSTSDPDEGEAFVAARRFAIPAVEAKGALLLEDVGVPVPRLADLCAGIEKIAA
HCCCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHC
DRDVMISVIAHAGDGNTHPLIVFDPADAAMAERAETAFGEIMDLAVGLGGTITGEHGVGR
CHHHEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
LKRPWLAGQIGPEAMELNRRIKQALDPDAILNPGAAF
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCCCC
>Mature Secondary Structure 
ADVNSALAELIADLSDGVVVTDPDIVASYRQDRAADPSAGTALAVVRPTRTEEVQTVLR
CCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHH
WASAHKVAVVPRGMGTGLSGGATALDGAIVLSTEKMRDITVDPVTRTAVAQPGLLNAEVK
HHCCCEEEEEECCCCCCCCCCCHHHCCEEEEECHHHCCCCCCCCHHHHHCCCCCCCHHHH
KAVAEYGLWYPPDPSSYEICSIGGNVATNAGGLCCVKYGVTTDYVLGLQVVLADGTAVRL
HHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHEEEEEEEEECCCEEEE
GGPRLKDVAGLSLTKLFVGSEGTLGVITEVTLRLLPPQPSPCTVVATFDSVEAAAGAVVT
CCCCHHHHCCCEEEEEEECCCCCEEHHEEEEHEECCCCCCCEEEEEECCCHHHHCCEEEE
ITGRIRPSMLEFMDAVAINAVEDKLRMGLDRNAAAMMVAASDDRGAAGAEDAEFMAKVFT
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
EAGATEVFSTSDPDEGEAFVAARRFAIPAVEAKGALLLEDVGVPVPRLADLCAGIEKIAA
HCCCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHC
DRDVMISVIAHAGDGNTHPLIVFDPADAAMAERAETAFGEIMDLAVGLGGTITGEHGVGR
CHHHEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
LKRPWLAGQIGPEAMELNRRIKQALDPDAILNPGAAF
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]