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The map label for this gene is rffG [C]

Identifier: 119869864

GI number: 119869864

Start: 4047769

End: 4048836

Strand: Direct

Name: rffG [C]

Synonym: Mkms_3833

Alternate gene names: 119869864

Gene position: 4047769-4048836 (Clockwise)

Preceding gene: 119869861

Following gene: 119869892

Centisome position: 70.55

GC content: 71.16

Gene sequence:

>1068_bases
ATGTCAGATGCGGTGCTCGTAACCGGCGGTTTCGGCCTGGTCGGTTCGCAAACGGTGCGGCGACTCGTCGAACTCGGCCG
GCGGGTGGTCGCCACCGACCTGCAGACCGACGCCAACCGGAAGGCCGCCGGGTCTTTGCCCGACGGCGCCGAGGTGCGCT
GGGCGGACCTGACCGACTCGGACCAGGTCGACCAGCTCCTCACCGAGGTGGCTCCGTCCGCGATCATCCATCTGGCCGCC
GTGATCCCGCCGCCGATCTACCGCAACCCGAAGGTGGCCCGCAAGGTCAACGTGGACGCCACCGCGACGCTGGTGCGGGC
GGCCGAAGCGCTGCCGCAGCCGCCCCGTTTCGTGCAGGCTTCGAGCAATGCGGTGTACGGCGCGCGTAACCCGCACCGGC
ACAAGGCCCCGGTGACCGCGGAGACCCCGCCGAACCCGACCGAGCTGTACGGCGCGCACAAGCTCGAAGCCGAGGAGATC
GTGCGCGCGTCGAGCCTCGAGTGGGTGGTGCTGCGCCTCGGCGGTGTGCTCAGCGTCGATCCGAAGGCCATGCCGTTGAC
CACCGACGCGTTGTTCTTCGAGAGCGCACTGCCCACCGACGGCCGGCTCCACAGCGTCGACGTGCGCGACGTGGCGTGGG
CGTTCGCGGCGGCCACCACCGCCGACGTCGTCGGCGAGATCCTGCTCATCGCCGGCGACGACACCCACAAGCGGCGCCAG
GGTGAGGTCGGACCCGCCCTCGCCGGCGCGCGCGGGTTGGCCGGCGCGCTGCCGCCAGGCCGTCCCGGTGATCCCGAGCG
GGACGACGCCTGGTTCATCACCGACTGGATGGATGCCACGCGCGCACAGGAAGCCCTTCAGTTCCAACATCATTCGTGGC
CCGACATGGTCGCCGAGATGAGCGGCCAGGTAGGGTTGCTGCGCTATCCGCTGCAGCTGATCGCACCGCTGGCGCGGGTG
TTCGTCGCCCGCCAGGGCGCCTACCGGAACAAGCCCGGCAAGTACGCCGACCCGTGGGCGGCGATCCGCTCCCGGCTCGG
CGACCCGGGACTCGACCGGCCCCGTTAA

Upstream 100 bases:

>100_bases
CTGACGGCTGTCGATCCTTCCGGATCAGCGAATACGTTACTCTCGCTAAAGGAGAACACCATAATCACCTGTTCTTGCTG
ACTGACCTCGTAGGAGAACA

Downstream 100 bases:

>100_bases
CCGGGGCCCAGGGTGGCCGCACCGGTCCGCGGATGCCGGTACCAGCCGCCCGCCGCGTGGGTTCCGCCGTCGACGTGCAG
CGTCTCACCGGTGATGTATC

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: Dtdp-4-Dehydrorhamnose Reductase; 3-Beta Hydroxysteroid Dehydrogenase/Isomerase Family; UDP-Glucose 4-Epimerase; UDP-Galactose 4-Epimerase; Nucleoside-Diphosphate-Sugar Epimerase

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSDAVLVTGGFGLVGSQTVRRLVELGRRVVATDLQTDANRKAAGSLPDGAEVRWADLTDSDQVDQLLTEVAPSAIIHLAA
VIPPPIYRNPKVARKVNVDATATLVRAAEALPQPPRFVQASSNAVYGARNPHRHKAPVTAETPPNPTELYGAHKLEAEEI
VRASSLEWVVLRLGGVLSVDPKAMPLTTDALFFESALPTDGRLHSVDVRDVAWAFAAATTADVVGEILLIAGDDTHKRRQ
GEVGPALAGARGLAGALPPGRPGDPERDDAWFITDWMDATRAQEALQFQHHSWPDMVAEMSGQVGLLRYPLQLIAPLARV
FVARQGAYRNKPGKYADPWAAIRSRLGDPGLDRPR

Sequences:

>Translated_355_residues
MSDAVLVTGGFGLVGSQTVRRLVELGRRVVATDLQTDANRKAAGSLPDGAEVRWADLTDSDQVDQLLTEVAPSAIIHLAA
VIPPPIYRNPKVARKVNVDATATLVRAAEALPQPPRFVQASSNAVYGARNPHRHKAPVTAETPPNPTELYGAHKLEAEEI
VRASSLEWVVLRLGGVLSVDPKAMPLTTDALFFESALPTDGRLHSVDVRDVAWAFAAATTADVVGEILLIAGDDTHKRRQ
GEVGPALAGARGLAGALPPGRPGDPERDDAWFITDWMDATRAQEALQFQHHSWPDMVAEMSGQVGLLRYPLQLIAPLARV
FVARQGAYRNKPGKYADPWAAIRSRLGDPGLDRPR
>Mature_354_residues
SDAVLVTGGFGLVGSQTVRRLVELGRRVVATDLQTDANRKAAGSLPDGAEVRWADLTDSDQVDQLLTEVAPSAIIHLAAV
IPPPIYRNPKVARKVNVDATATLVRAAEALPQPPRFVQASSNAVYGARNPHRHKAPVTAETPPNPTELYGAHKLEAEEIV
RASSLEWVVLRLGGVLSVDPKAMPLTTDALFFESALPTDGRLHSVDVRDVAWAFAAATTADVVGEILLIAGDDTHKRRQG
EVGPALAGARGLAGALPPGRPGDPERDDAWFITDWMDATRAQEALQFQHHSWPDMVAEMSGQVGLLRYPLQLIAPLARVF
VARQGAYRNKPGKYADPWAAIRSRLGDPGLDRPR

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38237; Mature: 38106

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAVLVTGGFGLVGSQTVRRLVELGRRVVATDLQTDANRKAAGSLPDGAEVRWADLTDS
CCCCEEEECCCCCCCHHHHHHHHHHCHHEEHHHHHCCCCCCCCCCCCCCCCEEEECCCCC
DQVDQLLTEVAPSAIIHLAAVIPPPIYRNPKVARKVNVDATATLVRAAEALPQPPRFVQA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEECCCHHHHHHHHHHHCCCCCCCEEC
SSNAVYGARNPHRHKAPVTAETPPNPTELYGAHKLEAEEIVRASSLEWVVLRLGGVLSVD
CCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHCCCCEEEEECCCEEEEC
PKAMPLTTDALFFESALPTDGRLHSVDVRDVAWAFAAATTADVVGEILLIAGDDTHKRRQ
CCCCCCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHC
GEVGPALAGARGLAGALPPGRPGDPERDDAWFITDWMDATRAQEALQFQHHSWPDMVAEM
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHH
SGQVGLLRYPLQLIAPLARVFVARQGAYRNKPGKYADPWAAIRSRLGDPGLDRPR
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SDAVLVTGGFGLVGSQTVRRLVELGRRVVATDLQTDANRKAAGSLPDGAEVRWADLTDS
CCCEEEECCCCCCCHHHHHHHHHHCHHEEHHHHHCCCCCCCCCCCCCCCCEEEECCCCC
DQVDQLLTEVAPSAIIHLAAVIPPPIYRNPKVARKVNVDATATLVRAAEALPQPPRFVQA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEECCCHHHHHHHHHHHCCCCCCCEEC
SSNAVYGARNPHRHKAPVTAETPPNPTELYGAHKLEAEEIVRASSLEWVVLRLGGVLSVD
CCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHCCCCEEEEECCCEEEEC
PKAMPLTTDALFFESALPTDGRLHSVDVRDVAWAFAAATTADVVGEILLIAGDDTHKRRQ
CCCCCCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHC
GEVGPALAGARGLAGALPPGRPGDPERDDAWFITDWMDATRAQEALQFQHHSWPDMVAEM
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHH
SGQVGLLRYPLQLIAPLARVFVARQGAYRNKPGKYADPWAAIRSRLGDPGLDRPR
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA