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The map label for this gene is 119869861

Identifier: 119869861

GI number: 119869861

Start: 4043738

End: 4045189

Strand: Direct

Name: 119869861

Synonym: Mkms_3830

Alternate gene names: NA

Gene position: 4043738-4045189 (Clockwise)

Preceding gene: 119869860

Following gene: 119869864

Centisome position: 70.48

GC content: 68.46

Gene sequence:

>1452_bases
GTGAAGCGACTCAACGGCATGGACGCGATGCTGCTGTACAGCGAGACCCCGAATCTGCACACCCACACCCTCAAGGTGGC
GATCATCGACGCCACCTCCTACGACGCCACGCACGACAGCCCGTACTCCTTCGAGGTGTTCCGCCGCACCATCGCCCGCC
GACTGCACCTCCTGGACCCGCTGCGCTACCGACTGATCGACATCCCGTTCCGCTTCCACCACCCGATGTGGCTGGAGAAC
TGCGATGTCGACCTGGACTACCACGTCCGCACGGTGTCGGTGCCCGCCCCCGGCGGCCGCCGGGAACTCGACGAAGTCAT
CGGGCGCATCGCCAGCACCCCGCTCGACCGCGACCGGCCGCTCTGGGAGTTCTACTTCGCCGAGGGGATGGCCGACAATC
GGTTCGCCCTGATCGGCAAGGTGCACCACACCCTGGCCGACGGCGTCGCGTCAGCGAACTTGCTGGCGCGGCTGATGGAT
CTGGGCGGTACCGTGACCCCCGAACGCGACGAGTACACCACCTGTCAGCCCCCGACGAAATCGCAGTTGCTCGCCGAAGC
CAGCCGCGACCATCTGCAGAAGGTGGCCGAGTTCCCCGGCCTGGTCGGCGAGGCGGTGCGCGGCACCTTCCGGGTCCGGC
GGCGCACACACGATCAGCGCGGTGGCACCGACGAGATGGCCAAGATGTTCCGGACGCCGCCCACCTTCCTCAACCACGTG
GTGTCGCCCGGGCGAACCTTCGCCACCGCATCGGTGGCGCTGGCGGACATCAAGGAGACCGCCCGGCACCTCGGGGTGAC
GTTCAACGACGTGGTGTTGTCGGTCAGCGCCGGCGGGTTACGCGAACTGTTGCTGCGCTACGACGGCCGCGCCGACCGCC
CCCTGCTGGCGTCGGTGCCCGTGGCCACCGACCGCTCCCCCGACCGGGTGACCGGGAACGAGATCAGCGGGCTGGCGGTG
TCGTTGCCCGTCCACGTCGACGATCCGTTGCGCCGGGTGCGGTTGACCGCGTTGGCCACCAAGCGCGCCAAGGAAGTTCA
CGATCTGATGGGGCCCCGGCTGCAAGGAAAGATGATGGAGTACCTGCCGCCGTTCGCTCCCACGGCGTTCCGCTGGCAGT
CCGAGCACGCCACCCACAACCGCATCATGAACGTCGCGGTGTCCAGCGTGCCGGGCCCGCGCGAACACGGACACATCGGC
GGCGGCGACGTCACCGAGATCTACTCGGTCGGGGTGCTTTCGGCGGCGAGCGCCTTCAACATGACCGTGTGGAGTTATGT
CGACCAGGTGGACATCTCTGTGCTGTCCGACGACCAGACCTTCCGCGACATCCACGAGGCGACCGATGCGATGCTGCACG
CGCTGGTCGAGATTCGCAGCGCCGCAGGGCTGCCCGCGGAACTCCGCCGGGTCGACACCGCCATGGCGCCGGCGAGCGCC
GCCGTCGGCTAG

Upstream 100 bases:

>100_bases
TTCGGGCGAAACATGCGCCACCGTCGCGCCCGGATGGTTGCATGGACTTGCGCAATCGCGGGGATACCTCCTTTACCGGC
GGCGAAGACGGAGGTGGGGC

Downstream 100 bases:

>100_bases
TTCAGGTGGGCGCGCAGTTCGTTGCGCAGCACCTTGCCGGTGCCGCCCCTGGGTAGCTCGTCGAGAATCACGATGTCGCG
CGGCACTTTGTAATTCGCCA

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv0221/MT0231 [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKRLNGMDAMLLYSETPNLHTHTLKVAIIDATSYDATHDSPYSFEVFRRTIARRLHLLDPLRYRLIDIPFRFHHPMWLEN
CDVDLDYHVRTVSVPAPGGRRELDEVIGRIASTPLDRDRPLWEFYFAEGMADNRFALIGKVHHTLADGVASANLLARLMD
LGGTVTPERDEYTTCQPPTKSQLLAEASRDHLQKVAEFPGLVGEAVRGTFRVRRRTHDQRGGTDEMAKMFRTPPTFLNHV
VSPGRTFATASVALADIKETARHLGVTFNDVVLSVSAGGLRELLLRYDGRADRPLLASVPVATDRSPDRVTGNEISGLAV
SLPVHVDDPLRRVRLTALATKRAKEVHDLMGPRLQGKMMEYLPPFAPTAFRWQSEHATHNRIMNVAVSSVPGPREHGHIG
GGDVTEIYSVGVLSAASAFNMTVWSYVDQVDISVLSDDQTFRDIHEATDAMLHALVEIRSAAGLPAELRRVDTAMAPASA
AVG

Sequences:

>Translated_483_residues
MKRLNGMDAMLLYSETPNLHTHTLKVAIIDATSYDATHDSPYSFEVFRRTIARRLHLLDPLRYRLIDIPFRFHHPMWLEN
CDVDLDYHVRTVSVPAPGGRRELDEVIGRIASTPLDRDRPLWEFYFAEGMADNRFALIGKVHHTLADGVASANLLARLMD
LGGTVTPERDEYTTCQPPTKSQLLAEASRDHLQKVAEFPGLVGEAVRGTFRVRRRTHDQRGGTDEMAKMFRTPPTFLNHV
VSPGRTFATASVALADIKETARHLGVTFNDVVLSVSAGGLRELLLRYDGRADRPLLASVPVATDRSPDRVTGNEISGLAV
SLPVHVDDPLRRVRLTALATKRAKEVHDLMGPRLQGKMMEYLPPFAPTAFRWQSEHATHNRIMNVAVSSVPGPREHGHIG
GGDVTEIYSVGVLSAASAFNMTVWSYVDQVDISVLSDDQTFRDIHEATDAMLHALVEIRSAAGLPAELRRVDTAMAPASA
AVG
>Mature_483_residues
MKRLNGMDAMLLYSETPNLHTHTLKVAIIDATSYDATHDSPYSFEVFRRTIARRLHLLDPLRYRLIDIPFRFHHPMWLEN
CDVDLDYHVRTVSVPAPGGRRELDEVIGRIASTPLDRDRPLWEFYFAEGMADNRFALIGKVHHTLADGVASANLLARLMD
LGGTVTPERDEYTTCQPPTKSQLLAEASRDHLQKVAEFPGLVGEAVRGTFRVRRRTHDQRGGTDEMAKMFRTPPTFLNHV
VSPGRTFATASVALADIKETARHLGVTFNDVVLSVSAGGLRELLLRYDGRADRPLLASVPVATDRSPDRVTGNEISGLAV
SLPVHVDDPLRRVRLTALATKRAKEVHDLMGPRLQGKMMEYLPPFAPTAFRWQSEHATHNRIMNVAVSSVPGPREHGHIG
GGDVTEIYSVGVLSAASAFNMTVWSYVDQVDISVLSDDQTFRDIHEATDAMLHALVEIRSAAGLPAELRRVDTAMAPASA
AVG

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmit

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255 [H]

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 53481; Mature: 53481

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLNGMDAMLLYSETPNLHTHTLKVAIIDATSYDATHDSPYSFEVFRRTIARRLHLLDP
CCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCH
LRYRLIDIPFRFHHPMWLENCDVDLDYHVRTVSVPAPGGRRELDEVIGRIASTPLDRDRP
HHHHEEECCHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCC
LWEFYFAEGMADNRFALIGKVHHTLADGVASANLLARLMDLGGTVTPERDEYTTCQPPTK
HHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
SQLLAEASRDHLQKVAEFPGLVGEAVRGTFRVRRRTHDQRGGTDEMAKMFRTPPTFLNHV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHH
VSPGRTFATASVALADIKETARHLGVTFNDVVLSVSAGGLRELLLRYDGRADRPLLASVP
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHHHHCCCCCCCCEEECC
VATDRSPDRVTGNEISGLAVSLPVHVDDPLRRVRLTALATKRAKEVHDLMGPRLQGKMME
CCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
YLPPFAPTAFRWQSEHATHNRIMNVAVSSVPGPREHGHIGGGDVTEIYSVGVLSAASAFN
HCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
MTVWSYVDQVDISVLSDDQTFRDIHEATDAMLHALVEIRSAAGLPAELRRVDTAMAPASA
HHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHH
AVG
CCC
>Mature Secondary Structure
MKRLNGMDAMLLYSETPNLHTHTLKVAIIDATSYDATHDSPYSFEVFRRTIARRLHLLDP
CCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCH
LRYRLIDIPFRFHHPMWLENCDVDLDYHVRTVSVPAPGGRRELDEVIGRIASTPLDRDRP
HHHHEEECCHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCC
LWEFYFAEGMADNRFALIGKVHHTLADGVASANLLARLMDLGGTVTPERDEYTTCQPPTK
HHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
SQLLAEASRDHLQKVAEFPGLVGEAVRGTFRVRRRTHDQRGGTDEMAKMFRTPPTFLNHV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHH
VSPGRTFATASVALADIKETARHLGVTFNDVVLSVSAGGLRELLLRYDGRADRPLLASVP
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHHHHCCCCCCCCEEECC
VATDRSPDRVTGNEISGLAVSLPVHVDDPLRRVRLTALATKRAKEVHDLMGPRLQGKMME
CCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
YLPPFAPTAFRWQSEHATHNRIMNVAVSSVPGPREHGHIGGGDVTEIYSVGVLSAASAFN
HCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
MTVWSYVDQVDISVLSDDQTFRDIHEATDAMLHALVEIRSAAGLPAELRRVDTAMAPASA
HHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHH
AVG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]