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The map label for this gene is fadE25 [H]

Identifier: 119869855

GI number: 119869855

Start: 4037728

End: 4038870

Strand: Direct

Name: fadE25 [H]

Synonym: Mkms_3824

Alternate gene names: 119869855

Gene position: 4037728-4038870 (Clockwise)

Preceding gene: 119869854

Following gene: 119869856

Centisome position: 70.38

GC content: 67.63

Gene sequence:

>1143_bases
ATGACCGAAGCCGTTCAGTACTCCGATCCCGCCGAGTTCCGGTCTCAGCTGGTGGCCTGGCTCGGCGAGAACGATCTGAC
CCCACCCGAGGACCATTCGCTGGAAGGCCACATCCGGCAGTTCGCCCGGGTGCAGAGGGCGCTCTACGACGCGGGCTGGA
TGCGCTACGGGTGGCCCGAGAGCGCGGGCGGCCTCGGCGGACCGGCGATCCTTCGGGCCATCGTCGGCGAGGAGGTGGTC
GGTCGCCGACTGGCCGAACCTGGGCCGTACTCGATGCTCGAGGTGCTGGCGCCGACGATGATCGACTACGCCGCCCCCGA
TCTGGCCGCCGAGATGGTGCCGAAACTGCTCAGCGGCGAAGAGCAGTGGTGCCAAGGGTTCTCCGAACCCGGTTCGGGTA
GCGATCTGGCATCGCTGACCACCCGCGCCGTGCTCGATCCCTCATCCGGGGGGAACACCTACGTCGTCAACGGCCAGAAG
GTCTGGACCAGCTTCGCGCAGTACTCCCACCGGTGCATCCTGCTGACCCGCACCGGCGATGCGAACACGCCGAACCACGA
GGCGATCACCGCGTTCTTCGTCGACCTCGACACCCCGGGTATCACCGTGCGGCCGCTGCGCACCATGCACGACGTCGACG
AATTCTGCGAGGTCTACTTCGACGACGTCAGGGTGGACGCGAGCCGGATGCTCGGCAAACCGGGCGACGGTTGGCGGCTG
GCGATGGATCTGCTGCCTTACGAACGCTCCACGTGCTTCTGGCAGCGCATCGCCTACCTGTACTCGCGGTTCGACGCGCT
CGTCGAGGAAGTCAAGCGGGTCGGTCAGGCCGTGGACACCGACATGGGCGACGTGTACCTGGCGCTGCACACGCTGCGGT
GCCGTTCCCGCGCCACCCAGCACCGGCTGGCCGACGGTCAGAAACTCGGGCCGGACACCTCCATCGACAAGGTCCTGCTG
GCCGGCGCCGAACAGCTCCTCTACGACACCGCGCGTGATCTGCTGCCCGGCGCCATCGAACTCGACGAGACCGAATGGCG
CACCGAGTACCTGTACTCGCGGGCGGCCACCATCTACGGCGGCACCGCCGAGGTGCAGCGCAACATCATCGCCCGCCGCC
TGCTCGACCTCGGGAAGGAGTGA

Upstream 100 bases:

>100_bases
ATGGCGGCTGGCGTCTGCGCACCCGCGCCATGACGTTCATCCGGCGCAGCGGCGAACACGACTCCGGTCGCCCCCACGCC
ATCGGAAGGCCCGAGGCCGG

Downstream 100 bases:

>100_bases
TCGTGGACCAGCAGTCACTCGAGATGCTCGAAACCTCACTGCGCAAGACCATGCTGTCGACATCGGGGCCCGATCTCGAC
GCCGCGCTCACCGAACTCGG

Product: acyl-CoA dehydrogenase domain-containing protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTEAVQYSDPAEFRSQLVAWLGENDLTPPEDHSLEGHIRQFARVQRALYDAGWMRYGWPESAGGLGGPAILRAIVGEEVV
GRRLAEPGPYSMLEVLAPTMIDYAAPDLAAEMVPKLLSGEEQWCQGFSEPGSGSDLASLTTRAVLDPSSGGNTYVVNGQK
VWTSFAQYSHRCILLTRTGDANTPNHEAITAFFVDLDTPGITVRPLRTMHDVDEFCEVYFDDVRVDASRMLGKPGDGWRL
AMDLLPYERSTCFWQRIAYLYSRFDALVEEVKRVGQAVDTDMGDVYLALHTLRCRSRATQHRLADGQKLGPDTSIDKVLL
AGAEQLLYDTARDLLPGAIELDETEWRTEYLYSRAATIYGGTAEVQRNIIARRLLDLGKE

Sequences:

>Translated_380_residues
MTEAVQYSDPAEFRSQLVAWLGENDLTPPEDHSLEGHIRQFARVQRALYDAGWMRYGWPESAGGLGGPAILRAIVGEEVV
GRRLAEPGPYSMLEVLAPTMIDYAAPDLAAEMVPKLLSGEEQWCQGFSEPGSGSDLASLTTRAVLDPSSGGNTYVVNGQK
VWTSFAQYSHRCILLTRTGDANTPNHEAITAFFVDLDTPGITVRPLRTMHDVDEFCEVYFDDVRVDASRMLGKPGDGWRL
AMDLLPYERSTCFWQRIAYLYSRFDALVEEVKRVGQAVDTDMGDVYLALHTLRCRSRATQHRLADGQKLGPDTSIDKVLL
AGAEQLLYDTARDLLPGAIELDETEWRTEYLYSRAATIYGGTAEVQRNIIARRLLDLGKE
>Mature_379_residues
TEAVQYSDPAEFRSQLVAWLGENDLTPPEDHSLEGHIRQFARVQRALYDAGWMRYGWPESAGGLGGPAILRAIVGEEVVG
RRLAEPGPYSMLEVLAPTMIDYAAPDLAAEMVPKLLSGEEQWCQGFSEPGSGSDLASLTTRAVLDPSSGGNTYVVNGQKV
WTSFAQYSHRCILLTRTGDANTPNHEAITAFFVDLDTPGITVRPLRTMHDVDEFCEVYFDDVRVDASRMLGKPGDGWRLA
MDLLPYERSTCFWQRIAYLYSRFDALVEEVKRVGQAVDTDMGDVYLALHTLRCRSRATQHRLADGQKLGPDTSIDKVLLA
GAEQLLYDTARDLLPGAIELDETEWRTEYLYSRAATIYGGTAEVQRNIIARRLLDLGKE

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=371, Percent_Identity=26.4150943396226, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI4501857, Length=330, Percent_Identity=27.2727272727273, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI187960098, Length=348, Percent_Identity=25.8620689655172, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI4557231, Length=348, Percent_Identity=25.8620689655172, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI21361497, Length=213, Percent_Identity=28.6384976525822, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4557235, Length=142, Percent_Identity=37.3239436619718, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI76496475, Length=142, Percent_Identity=37.3239436619718, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI226958414, Length=284, Percent_Identity=27.112676056338, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI226958412, Length=284, Percent_Identity=27.112676056338, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4501859, Length=343, Percent_Identity=23.9067055393586, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1786223, Length=348, Percent_Identity=24.7126436781609, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081958, Length=342, Percent_Identity=25.4385964912281, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17570075, Length=386, Percent_Identity=26.4248704663212, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17569725, Length=368, Percent_Identity=27.1739130434783, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508101, Length=386, Percent_Identity=24.3523316062176, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17534899, Length=355, Percent_Identity=26.4788732394366, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17506239, Length=255, Percent_Identity=25.8823529411765, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71990804, Length=237, Percent_Identity=29.1139240506329, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17538396, Length=396, Percent_Identity=26.010101010101, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71985184, Length=141, Percent_Identity=30.4964539007092, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71985192, Length=141, Percent_Identity=30.4964539007092, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32563615, Length=220, Percent_Identity=27.7272727272727, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21356377, Length=366, Percent_Identity=24.0437158469945, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21355753, Length=334, Percent_Identity=26.9461077844311, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24660351, Length=266, Percent_Identity=27.4436090225564, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281363737, Length=360, Percent_Identity=25.8333333333333, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24646207, Length=274, Percent_Identity=26.6423357664234, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24666513, Length=244, Percent_Identity=27.4590163934426, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 42303; Mature: 42172

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAVQYSDPAEFRSQLVAWLGENDLTPPEDHSLEGHIRQFARVQRALYDAGWMRYGWPE
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
SAGGLGGPAILRAIVGEEVVGRRLAEPGPYSMLEVLAPTMIDYAAPDLAAEMVPKLLSGE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
EQWCQGFSEPGSGSDLASLTTRAVLDPSSGGNTYVVNGQKVWTSFAQYSHRCILLTRTGD
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCEEEEEEECCC
ANTPNHEAITAFFVDLDTPGITVRPLRTMHDVDEFCEVYFDDVRVDASRMLGKPGDGWRL
CCCCCCCEEEEEEEEECCCCCEECCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEE
AMDLLPYERSTCFWQRIAYLYSRFDALVEEVKRVGQAVDTDMGDVYLALHTLRCRSRATQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HRLADGQKLGPDTSIDKVLLAGAEQLLYDTARDLLPGAIELDETEWRTEYLYSRAATIYG
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHCC
GTAEVQRNIIARRLLDLGKE
CHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEAVQYSDPAEFRSQLVAWLGENDLTPPEDHSLEGHIRQFARVQRALYDAGWMRYGWPE
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
SAGGLGGPAILRAIVGEEVVGRRLAEPGPYSMLEVLAPTMIDYAAPDLAAEMVPKLLSGE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
EQWCQGFSEPGSGSDLASLTTRAVLDPSSGGNTYVVNGQKVWTSFAQYSHRCILLTRTGD
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCEEEEEEECCC
ANTPNHEAITAFFVDLDTPGITVRPLRTMHDVDEFCEVYFDDVRVDASRMLGKPGDGWRL
CCCCCCCEEEEEEEEECCCCCEECCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEE
AMDLLPYERSTCFWQRIAYLYSRFDALVEEVKRVGQAVDTDMGDVYLALHTLRCRSRATQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HRLADGQKLGPDTSIDKVLLAGAEQLLYDTARDLLPGAIELDETEWRTEYLYSRAATIYG
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHCC
GTAEVQRNIIARRLLDLGKE
CHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]