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The map label for this gene is nuoF2 [H]

Identifier: 119869794

GI number: 119869794

Start: 3980965

End: 3982266

Strand: Direct

Name: nuoF2 [H]

Synonym: Mkms_3763

Alternate gene names: 119869794

Gene position: 3980965-3982266 (Clockwise)

Preceding gene: 119869792

Following gene: 119869795

Centisome position: 69.39

GC content: 72.04

Gene sequence:

>1302_bases
ATGAACCCCACCGCCGCAACCGATCTCACCACCGCCGTCTGGCCGGGCACCACGCCGCGTCTGCTGCACGTGCCCGCCGG
GCGGGAGGACTACGCCGACTACGCGCAGTCCGGCGGCTACCGCGAGCTGCCCGACCCCGAACGACTGCTCGAGGAAGTCG
AACTGTCCGGCCTGCTCGGACGTGGCGGTGCCGCGTTCCCGATGGCGGTCAAACTGCGCTCGGTGCGCGATCACGGCCGT
ACCCGCGGCGGCGCCGTCGCGATCGCCAACGGCGAAGAGGGCGAACCCGCGTCGATCAAGGACCGGTGGCTGCTGCGGCA
CCGCCCGCACCTTGTGCTCGACGGTCTGCGCCTGGCCGCGCGCGTCGTGGAGGCCGAGCGCGCAATCGTCTACGTCTCCG
ATCCGGAGTCCGCCCGCAGTGTCGAAACCGCGCTGACACAGGTCGATTCACCGGTTCTGGACGGTGTGTCGATCAGCGTC
GTGGTCGTCGACCCCGGTTACGTCGCCGGTGAGGAGACCGCCGCCGTCCGTGCCATCGACGGGGGGCCCGCCAAACCGAC
GGACAAGCCGCCGCGGCCGTTCGAGGAGGGGGTGGGTGGGCTGCCCACACTGGTCAGCAACGTCGAGACGCTCGCCAACC
TGCCGTATCTGCACGAGCACGGGGCGCGGGAGTACCGGTCGGCGGGCACACCGTCGTCCCCCGGCACCTTCCTGGCGACC
GTCACCGGCGGTGGCGGGCCACCGGCGCTCTACGAGTTGCCGCACGGTGTCGCCCTCGCCGATGTGCTGACCTGGCACGG
GATCTCGCCCGATTCGGTCAGCGGCGTGCTGATGGGCGGTTACTTCACCGGGCTGCTCAACCGTGACGTCCTCGACGCGA
CGCTCGACCACGAGGCGCTGCGCCGCCTCGGCAGCGGTCTGGGCTGCGGGGCGGTCGGGGTGCTGACCGACGAATGCCCC
GTCGCGGTGGCCGCATCGGTGCTGGCCTACTTCGACCGCGAGAACGCCGGACAGTGCGGATCCTGTTTCAACGGGACCGC
CGCCATGGCGGCCGTGGCTGTGGCGCTGCGCGACGGCGTGGCCTCCGACGACGATCTGGCCCGGCTGGAGCGCTGGTCGG
TGGTGCTGCGGGGGCGGGGTGCGTGCGGGACGCTCGACGCGGCCACGAACGTCGCCGCCAGCCTGCTCACCGCGTTCCCG
CAGGCGGTGGCCCGGCACCGGGAGGGCAGCTGTGATCGCTGCGCCGTCGACCTGTACGACGTCCGACGACCCTACGAAGT
GGAGGCGGTGGTGAGCGCGTGA

Upstream 100 bases:

>100_bases
TAGCTGCGCGGGTGTCAGTTCGCCCGCCGGGTCCACCCCCACCCGCACCACCGCAGTTCTCATGTCGGCCAGCCTATCCG
CGAGGACAAAGTAGAAGCAG

Downstream 100 bases:

>100_bases
GGATCCGACTCGATCGCACGATGTGCGACGGCTTCGGCATCTGCGCCAAGCACGCACCGGAGTACTTCTCGCTCGACGAC
TGGGGTTACGCCTCGCTGAT

Product: respiratory-chain NADH dehydrogenase domain-containing protein

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNPTAATDLTTAVWPGTTPRLLHVPAGREDYADYAQSGGYRELPDPERLLEEVELSGLLGRGGAAFPMAVKLRSVRDHGR
TRGGAVAIANGEEGEPASIKDRWLLRHRPHLVLDGLRLAARVVEAERAIVYVSDPESARSVETALTQVDSPVLDGVSISV
VVVDPGYVAGEETAAVRAIDGGPAKPTDKPPRPFEEGVGGLPTLVSNVETLANLPYLHEHGAREYRSAGTPSSPGTFLAT
VTGGGGPPALYELPHGVALADVLTWHGISPDSVSGVLMGGYFTGLLNRDVLDATLDHEALRRLGSGLGCGAVGVLTDECP
VAVAASVLAYFDRENAGQCGSCFNGTAAMAAVAVALRDGVASDDDLARLERWSVVLRGRGACGTLDAATNVAASLLTAFP
QAVARHREGSCDRCAVDLYDVRRPYEVEAVVSA

Sequences:

>Translated_433_residues
MNPTAATDLTTAVWPGTTPRLLHVPAGREDYADYAQSGGYRELPDPERLLEEVELSGLLGRGGAAFPMAVKLRSVRDHGR
TRGGAVAIANGEEGEPASIKDRWLLRHRPHLVLDGLRLAARVVEAERAIVYVSDPESARSVETALTQVDSPVLDGVSISV
VVVDPGYVAGEETAAVRAIDGGPAKPTDKPPRPFEEGVGGLPTLVSNVETLANLPYLHEHGAREYRSAGTPSSPGTFLAT
VTGGGGPPALYELPHGVALADVLTWHGISPDSVSGVLMGGYFTGLLNRDVLDATLDHEALRRLGSGLGCGAVGVLTDECP
VAVAASVLAYFDRENAGQCGSCFNGTAAMAAVAVALRDGVASDDDLARLERWSVVLRGRGACGTLDAATNVAASLLTAFP
QAVARHREGSCDRCAVDLYDVRRPYEVEAVVSA
>Mature_433_residues
MNPTAATDLTTAVWPGTTPRLLHVPAGREDYADYAQSGGYRELPDPERLLEEVELSGLLGRGGAAFPMAVKLRSVRDHGR
TRGGAVAIANGEEGEPASIKDRWLLRHRPHLVLDGLRLAARVVEAERAIVYVSDPESARSVETALTQVDSPVLDGVSISV
VVVDPGYVAGEETAAVRAIDGGPAKPTDKPPRPFEEGVGGLPTLVSNVETLANLPYLHEHGAREYRSAGTPSSPGTFLAT
VTGGGGPPALYELPHGVALADVLTWHGISPDSVSGVLMGGYFTGLLNRDVLDATLDHEALRRLGSGLGCGAVGVLTDECP
VAVAASVLAYFDRENAGQCGSCFNGTAAMAAVAVALRDGVASDDDLARLERWSVVLRGRGACGTLDAATNVAASLLTAFP
QAVARHREGSCDRCAVDLYDVRRPYEVEAVVSA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=376, Percent_Identity=31.3829787234043, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI260656005, Length=376, Percent_Identity=31.3829787234043, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1788620, Length=341, Percent_Identity=29.9120234604106, Blast_Score=138, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17531727, Length=382, Percent_Identity=29.0575916230367, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24653819, Length=412, Percent_Identity=28.6407766990291, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI19920794, Length=383, Percent_Identity=28.4595300261097, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24653817, Length=339, Percent_Identity=28.9085545722714, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI45550452, Length=370, Percent_Identity=26.4864864864865, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 45284; Mature: 45284

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTAATDLTTAVWPGTTPRLLHVPAGREDYADYAQSGGYRELPDPERLLEEVELSGLLG
CCCCCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHC
RGGAAFPMAVKLRSVRDHGRTRGGAVAIANGEEGEPASIKDRWLLRHRPHLVLDGLRLAA
CCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
RVVEAERAIVYVSDPESARSVETALTQVDSPVLDGVSISVVVVDPGYVAGEETAAVRAID
HHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEC
GGPAKPTDKPPRPFEEGVGGLPTLVSNVETLANLPYLHEHGAREYRSAGTPSSPGTFLAT
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHCHHHHHCCCCCCCCCCEEEE
VTGGGGPPALYELPHGVALADVLTWHGISPDSVSGVLMGGYFTGLLNRDVLDATLDHEAL
EECCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCHHHHHHCCHHHH
RRLGSGLGCGAVGVLTDECPVAVAASVLAYFDRENAGQCGSCFNGTAAMAAVAVALRDGV
HHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ASDDDLARLERWSVVLRGRGACGTLDAATNVAASLLTAFPQAVARHREGSCDRCAVDLYD
CCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHH
VRRPYEVEAVVSA
CCCCCEEEEEECC
>Mature Secondary Structure
MNPTAATDLTTAVWPGTTPRLLHVPAGREDYADYAQSGGYRELPDPERLLEEVELSGLLG
CCCCCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHC
RGGAAFPMAVKLRSVRDHGRTRGGAVAIANGEEGEPASIKDRWLLRHRPHLVLDGLRLAA
CCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
RVVEAERAIVYVSDPESARSVETALTQVDSPVLDGVSISVVVVDPGYVAGEETAAVRAID
HHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEC
GGPAKPTDKPPRPFEEGVGGLPTLVSNVETLANLPYLHEHGAREYRSAGTPSSPGTFLAT
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHCHHHHHCCCCCCCCCCEEEE
VTGGGGPPALYELPHGVALADVLTWHGISPDSVSGVLMGGYFTGLLNRDVLDATLDHEAL
EECCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCHHHHHHCCHHHH
RRLGSGLGCGAVGVLTDECPVAVAASVLAYFDRENAGQCGSCFNGTAAMAAVAVALRDGV
HHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ASDDDLARLERWSVVLRGRGACGTLDAATNVAASLLTAFPQAVARHREGSCDRCAVDLYD
CCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHH
VRRPYEVEAVVSA
CCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]