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The map label for this gene is ppsA [H]

Identifier: 119869501

GI number: 119869501

Start: 3639470

End: 3641866

Strand: Direct

Name: ppsA [H]

Synonym: Mkms_3469

Alternate gene names: 119869501

Gene position: 3639470-3641866 (Clockwise)

Preceding gene: 119869500

Following gene: 119869502

Centisome position: 63.44

GC content: 67.25

Gene sequence:

>2397_bases
ATGAGTGGGTCCGCCTACGTCACCTTCTTCGAGGAGATCGGCATCGACGACGTGCCTCTGGTCGGCGGCAAGAACGCCTC
GCTGGGCGAGATGTACCAGAACCTGTCCGGCCAGGGTGTCCGTGTGCCACACGGGTTCGCGATCACCGCCGCGGCGTACC
GCCACATGCTCGACGAGGCCGACGCGTGGGGCCGGTTGCACGCCGAACTCGACGACATCGACCCCGCCGACGTCGCCGCC
CTGGCGCGCAAGGGTAAGCGCGCCCGCGAGATCGTCTACGGCGCAGGGCTACCCGACGATCTGGCCGCCCAGATCCGGGA
TGCCTACCGCACGCTCCAGCAGGAGTACGGCGAAGACGTCAGCCTCGCCGTCCGCAGTTCAGCGACCGCAGAGGACCTGC
CCACGGCGAGCTTCGCCGGCCAGCAGGACTCCTACCTCAACATCAAGGGCGACGAAAGCCTGCTGGACACCTGCCGCCGC
TGCTTCGCCAGCCTGTTCACCGATCGCGCGATCCACTACCGGATCGATCAGGGCTTCGACCATTTCAAGGTGTCGCTGTC
GATCGGCGTGATGAAGATGGTCCGCTCCGACATCGCCTCCTCCGGCGTGATGTTCTCGTTGGACACCGAATCGGGTTTCC
GCGACGCGGTGTTCCTCACCGGTGCCTACGGCCTCGGTGAGAACGTGGTCCAGGGCGCGGTCGACCCCGACGAGTTCTAT
GTCCACAAGCCCACCTATCTCGCCGGCCACCGCGCGGTCCTGCGCCGGCGCCTCGGCGACAAGGCCGTGAAGATGGTCTA
CGTCGAAGGCGAGACGAAGAACACCACCCGCAACATCCCCACCCCCAAGGCCGATCGCGCCCGCTTCTGCCTCGACGACG
AGGACGTCCTGGAACTGGCCGGCTACGCGTGTGCGATCGAACAGCACTACGGCCGGCCCATGGACATGGAGTGGGCCAAG
GACGGCCTCGACGGGCAGCTCTACATCGTCCAGGCCCGCCCCGAGACCGTGGCCTCCCAGCAGAGCGTCACCGCCCTGGA
GACCTATGTGTTGGAGAGCCACGCCGAGGTGCTGGCCGAGGGCCGCTCGGTCGGCGAGCGCGTCGCCTCCGGTGCGGTCA
AACGCATCGAGCACCTGTCCCAGCTGGCGGAGTTCCAGCCCGGGCAGGTGCTGGTGGCCGACACCACCACCCCCGACTGG
GAACCGGTCATGAAGACCGCCGCGGCGATCGTCACCAACCGCGGCGGACGGACCTGCCACGCGTCGATCATCGCCCGCGA
ACTCGGCATCCCGGCCGTCGTCGGGACCGGCGACGCGACCGTCAACGTGCCCGACGGCGCCATGGTGACGGTGTCGTGCG
CGGAAGGCGATACGGGCCGGGTGTACGACGGCGCGTTGGGTTTCCACGTGGACCGCACCGAGGTCGGCGACATCGCGCGG
CCGCGCACCGAGATCATGATCAACCTGGGCAACCCCGAACTCGCCTTCAAGACGTCGTTCCTGCCCAACGACGGTGTCGG
ATTGGCCCGGATGGAGTTCGTGATCAGCGAGTACATCCGTTTCCATCCGCTGGCGCTGCTGCACCCCGAGAAGGTCGACG
ACCCTGCGATCCGGCGTGACATCGAACGGCTGACGCAGGGCCACCGGGACGGGGGCGCCTTCTTCGTCGAGCGCCTGTCG
GAGGGGATCGGCACGATCGCGGCCGCGTTCTGGCCGAAGCCGGTGGTGGTGCGGATGTCGGATTTCAAGACCAACGAGTA
CGCGAGCCTGCTCGGCGGACGTGTATTCGAGCCCACGGAGAGCAACCCGATGCTCGGCTTCCGGGGGGCCTCGCGCTACG
CCCATCCCGCGTACGCGGAGGGCTTCGCCCTCGAGTGCCGGGCGATGCGGCGCGTACGCGAGGAGATGGGCCTGAGCAAC
GTCATCCTCATGCTGCCGTTCGTCCGCCGGGTCGCCGAGGCGGACCTGGTTCTCCAGACGATGGCCGACCTCGGTCTGCG
GCGAGGGGAGAACGGGCTGAAGATCTACGCGATGTGCGAGATCCCGAACAACGTCATCCTCATCGACGAGTTCGCCAAAC
GCTTCGACGGATTCTCCATCGGCTCCAACGACCTGACCCAGCTCACCCTGGGCGTCGATCGTGACAGCGAGGTCGTCGCG
TTCGACTACGACGAACGTGACGACGGCGTGAAGGAGATGATCCGGCTGGCCGTGGAAGGCTGTCGCCGCAACGGCATTCA
CTCGGGACTGTGCGGACAGGCCCCCTCTGACTACCCGGACATGGCCGAGTTCCTGGTGCGGGTCGGCATCGATTCGATGA
GCCTCAACCCCGACACCGTCGTGCGGACCACCCGCCTGGTCCTCGCCGCCGAACAACAGCTCGCATCCCTGTCATGA

Upstream 100 bases:

>100_bases
GCCCAAAGCCCCGAGTCCGCATTCGTGTGACTTCCGACCCTGGCTCGACCACACCGGACCGTGGCACGTTTGCATTCGTC
ACGCAACGAGAGGACAGATC

Downstream 100 bases:

>100_bases
CCGTGCCGGTCGTCATCGATCCCCGCTACCACGACGCGGTGATCTTCGACCTCGACGGCGTGCTCAGCGACCCGGCGCCG
ACGGTGACGCTGGCGCGCGA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 798; Mature: 797

Protein sequence:

>798_residues
MSGSAYVTFFEEIGIDDVPLVGGKNASLGEMYQNLSGQGVRVPHGFAITAAAYRHMLDEADAWGRLHAELDDIDPADVAA
LARKGKRAREIVYGAGLPDDLAAQIRDAYRTLQQEYGEDVSLAVRSSATAEDLPTASFAGQQDSYLNIKGDESLLDTCRR
CFASLFTDRAIHYRIDQGFDHFKVSLSIGVMKMVRSDIASSGVMFSLDTESGFRDAVFLTGAYGLGENVVQGAVDPDEFY
VHKPTYLAGHRAVLRRRLGDKAVKMVYVEGETKNTTRNIPTPKADRARFCLDDEDVLELAGYACAIEQHYGRPMDMEWAK
DGLDGQLYIVQARPETVASQQSVTALETYVLESHAEVLAEGRSVGERVASGAVKRIEHLSQLAEFQPGQVLVADTTTPDW
EPVMKTAAAIVTNRGGRTCHASIIARELGIPAVVGTGDATVNVPDGAMVTVSCAEGDTGRVYDGALGFHVDRTEVGDIAR
PRTEIMINLGNPELAFKTSFLPNDGVGLARMEFVISEYIRFHPLALLHPEKVDDPAIRRDIERLTQGHRDGGAFFVERLS
EGIGTIAAAFWPKPVVVRMSDFKTNEYASLLGGRVFEPTESNPMLGFRGASRYAHPAYAEGFALECRAMRRVREEMGLSN
VILMLPFVRRVAEADLVLQTMADLGLRRGENGLKIYAMCEIPNNVILIDEFAKRFDGFSIGSNDLTQLTLGVDRDSEVVA
FDYDERDDGVKEMIRLAVEGCRRNGIHSGLCGQAPSDYPDMAEFLVRVGIDSMSLNPDTVVRTTRLVLAAEQQLASLS

Sequences:

>Translated_798_residues
MSGSAYVTFFEEIGIDDVPLVGGKNASLGEMYQNLSGQGVRVPHGFAITAAAYRHMLDEADAWGRLHAELDDIDPADVAA
LARKGKRAREIVYGAGLPDDLAAQIRDAYRTLQQEYGEDVSLAVRSSATAEDLPTASFAGQQDSYLNIKGDESLLDTCRR
CFASLFTDRAIHYRIDQGFDHFKVSLSIGVMKMVRSDIASSGVMFSLDTESGFRDAVFLTGAYGLGENVVQGAVDPDEFY
VHKPTYLAGHRAVLRRRLGDKAVKMVYVEGETKNTTRNIPTPKADRARFCLDDEDVLELAGYACAIEQHYGRPMDMEWAK
DGLDGQLYIVQARPETVASQQSVTALETYVLESHAEVLAEGRSVGERVASGAVKRIEHLSQLAEFQPGQVLVADTTTPDW
EPVMKTAAAIVTNRGGRTCHASIIARELGIPAVVGTGDATVNVPDGAMVTVSCAEGDTGRVYDGALGFHVDRTEVGDIAR
PRTEIMINLGNPELAFKTSFLPNDGVGLARMEFVISEYIRFHPLALLHPEKVDDPAIRRDIERLTQGHRDGGAFFVERLS
EGIGTIAAAFWPKPVVVRMSDFKTNEYASLLGGRVFEPTESNPMLGFRGASRYAHPAYAEGFALECRAMRRVREEMGLSN
VILMLPFVRRVAEADLVLQTMADLGLRRGENGLKIYAMCEIPNNVILIDEFAKRFDGFSIGSNDLTQLTLGVDRDSEVVA
FDYDERDDGVKEMIRLAVEGCRRNGIHSGLCGQAPSDYPDMAEFLVRVGIDSMSLNPDTVVRTTRLVLAAEQQLASLS
>Mature_797_residues
SGSAYVTFFEEIGIDDVPLVGGKNASLGEMYQNLSGQGVRVPHGFAITAAAYRHMLDEADAWGRLHAELDDIDPADVAAL
ARKGKRAREIVYGAGLPDDLAAQIRDAYRTLQQEYGEDVSLAVRSSATAEDLPTASFAGQQDSYLNIKGDESLLDTCRRC
FASLFTDRAIHYRIDQGFDHFKVSLSIGVMKMVRSDIASSGVMFSLDTESGFRDAVFLTGAYGLGENVVQGAVDPDEFYV
HKPTYLAGHRAVLRRRLGDKAVKMVYVEGETKNTTRNIPTPKADRARFCLDDEDVLELAGYACAIEQHYGRPMDMEWAKD
GLDGQLYIVQARPETVASQQSVTALETYVLESHAEVLAEGRSVGERVASGAVKRIEHLSQLAEFQPGQVLVADTTTPDWE
PVMKTAAAIVTNRGGRTCHASIIARELGIPAVVGTGDATVNVPDGAMVTVSCAEGDTGRVYDGALGFHVDRTEVGDIARP
RTEIMINLGNPELAFKTSFLPNDGVGLARMEFVISEYIRFHPLALLHPEKVDDPAIRRDIERLTQGHRDGGAFFVERLSE
GIGTIAAAFWPKPVVVRMSDFKTNEYASLLGGRVFEPTESNPMLGFRGASRYAHPAYAEGFALECRAMRRVREEMGLSNV
ILMLPFVRRVAEADLVLQTMADLGLRRGENGLKIYAMCEIPNNVILIDEFAKRFDGFSIGSNDLTQLTLGVDRDSEVVAF
DYDERDDGVKEMIRLAVEGCRRNGIHSGLCGQAPSDYPDMAEFLVRVGIDSMSLNPDTVVRTTRLVLAAEQQLASLS

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=781, Percent_Identity=54.2893725992318, Blast_Score=852, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=491, Percent_Identity=24.4399185336049, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI48994992, Length=399, Percent_Identity=24.3107769423559, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1789193, Length=427, Percent_Identity=26.2295081967213, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788726, Length=194, Percent_Identity=29.8969072164948, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17564524, Length=328, Percent_Identity=25.9146341463415, Blast_Score=92, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87350; Mature: 87219

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSAYVTFFEEIGIDDVPLVGGKNASLGEMYQNLSGQGVRVPHGFAITAAAYRHMLDEA
CCCCEEEEEHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHH
DAWGRLHAELDDIDPADVAALARKGKRAREIVYGAGLPDDLAAQIRDAYRTLQQEYGEDV
HHHHHHHCCCCCCCHHHHHHHHHCCCHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCE
SLAVRSSATAEDLPTASFAGQQDSYLNIKGDESLLDTCRRCFASLFTDRAIHYRIDQGFD
EEEEECCCCHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCC
HFKVSLSIGVMKMVRSDIASSGVMFSLDTESGFRDAVFLTGAYGLGENVVQGAVDPDEFY
EEEEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCCHHHHCCCCCCCEE
VHKPTYLAGHRAVLRRRLGDKAVKMVYVEGETKNTTRNIPTPKADRARFCLDDEDVLELA
EECCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCEECCCCHHHHHHH
GYACAIEQHYGRPMDMEWAKDGLDGQLYIVQARPETVASQQSVTALETYVLESHAEVLAE
HHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
GRSVGERVASGAVKRIEHLSQLAEFQPGQVLVADTTTPDWEPVMKTAAAIVTNRGGRTCH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEH
ASIIARELGIPAVVGTGDATVNVPDGAMVTVSCAEGDTGRVYDGALGFHVDRTEVGDIAR
HHHHHHHCCCCEEEECCCEEEECCCCCEEEEEECCCCCCCEEECCEEEEECCCCCCCCCC
PRTEIMINLGNPELAFKTSFLPNDGVGLARMEFVISEYIRFHPLALLHPEKVDDPAIRRD
CCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHH
IERLTQGHRDGGAFFVERLSEGIGTIAAAFWPKPVVVRMSDFKTNEYASLLGGRVFEPTE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCEEECCCC
SNPMLGFRGASRYAHPAYAEGFALECRAMRRVREEMGLSNVILMLPFVRRVAEADLVLQT
CCCCEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MADLGLRRGENGLKIYAMCEIPNNVILIDEFAKRFDGFSIGSNDLTQLTLGVDRDSEVVA
HHHHHHHCCCCCCEEEEEEECCCCEEEEEHHHHHCCCCCCCCCCCEEEEEECCCCCCEEE
FDYDERDDGVKEMIRLAVEGCRRNGIHSGLCGQAPSDYPDMAEFLVRVGIDSMSLNPDTV
EECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
VRTTRLVLAAEQQLASLS
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGSAYVTFFEEIGIDDVPLVGGKNASLGEMYQNLSGQGVRVPHGFAITAAAYRHMLDEA
CCCEEEEEHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHH
DAWGRLHAELDDIDPADVAALARKGKRAREIVYGAGLPDDLAAQIRDAYRTLQQEYGEDV
HHHHHHHCCCCCCCHHHHHHHHHCCCHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCE
SLAVRSSATAEDLPTASFAGQQDSYLNIKGDESLLDTCRRCFASLFTDRAIHYRIDQGFD
EEEEECCCCHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCC
HFKVSLSIGVMKMVRSDIASSGVMFSLDTESGFRDAVFLTGAYGLGENVVQGAVDPDEFY
EEEEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCCHHHHCCCCCCCEE
VHKPTYLAGHRAVLRRRLGDKAVKMVYVEGETKNTTRNIPTPKADRARFCLDDEDVLELA
EECCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCEECCCCHHHHHHH
GYACAIEQHYGRPMDMEWAKDGLDGQLYIVQARPETVASQQSVTALETYVLESHAEVLAE
HHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
GRSVGERVASGAVKRIEHLSQLAEFQPGQVLVADTTTPDWEPVMKTAAAIVTNRGGRTCH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEH
ASIIARELGIPAVVGTGDATVNVPDGAMVTVSCAEGDTGRVYDGALGFHVDRTEVGDIAR
HHHHHHHCCCCEEEECCCEEEECCCCCEEEEEECCCCCCCEEECCEEEEECCCCCCCCCC
PRTEIMINLGNPELAFKTSFLPNDGVGLARMEFVISEYIRFHPLALLHPEKVDDPAIRRD
CCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHH
IERLTQGHRDGGAFFVERLSEGIGTIAAAFWPKPVVVRMSDFKTNEYASLLGGRVFEPTE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCEEECCCC
SNPMLGFRGASRYAHPAYAEGFALECRAMRRVREEMGLSNVILMLPFVRRVAEADLVLQT
CCCCEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MADLGLRRGENGLKIYAMCEIPNNVILIDEFAKRFDGFSIGSNDLTQLTLGVDRDSEVVA
HHHHHHHCCCCCCEEEEEEECCCCEEEEEHHHHHCCCCCCCCCCCEEEEEECCCCCCEEE
FDYDERDDGVKEMIRLAVEGCRRNGIHSGLCGQAPSDYPDMAEFLVRVGIDSMSLNPDTV
EECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
VRTTRLVLAAEQQLASLS
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]