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The map label for this gene is panB

Identifier: 119869426

GI number: 119869426

Start: 3561915

End: 3562763

Strand: Direct

Name: panB

Synonym: Mkms_3394

Alternate gene names: 119869426

Gene position: 3561915-3562763 (Clockwise)

Preceding gene: 119869419

Following gene: 119869428

Centisome position: 62.08

GC content: 68.67

Gene sequence:

>849_bases
ATGTCTGAGCAGACTGTCTACGGTGCTGCGAGCGATCAGCCCACCCCCAAGCCGCGCGTCAAGGTCCGCACCACCCACCT
GCAGAAGTGGAAGGCCGAAGGGCACAAGTGGGCGATGCTCACCGCCTACGACTTCTCCACCGCCCGGGCATTCGACGACG
CCGGCATTCCGGTGTTGCTCGTGGGCGACTCGGCCGCCAACGTCGTCTACGGCTACGACACCACGGTGCCGATCACGATC
GACGAGTTGATCCCACTGGTGCGCGGCGTGGTGCGGGGCGCACCGCATGCGTTGGTGGTGGCGGATCTGCCGTTCGGCAG
CTACGAGGAAGGCCCTCGCCAAGCCCTGGCCACGGCCACCCGGTTCCTCAAGGAGACCGGTGCGCACGCGGTCAAACTCG
AGGGCGGCGAACGCGTCGCCGAGCAGATCGCCACGCTGTCCGCGGCGGGCATCCCGGTCATGGCGCACATCGGCTTCACC
CCGCAGAGCGTCAACGGCCTGGGCGGCTTCAAGGTCCAGGGCCGTGGCGACGCCGCCGAGCAGACCATCCACGACGCGAT
CGCGGTCCAGGAGGCCGGCGCGTTCTCGGTGGTGATGGAGATGGTGCCCGCCGAACTGGCCACCCAGATCACCGGCAAGC
TGACCATCCCGACGGTCGGCATCGGCGCCGGGCCGAATTGCGATGCGCAGGTGCTGGTCTGGCAGGACATGGCCGGACTG
ACGTCGGGCAGGACGGCGAAGTTCGTGAAGCGCTTCGGCGATGTCGGCGCCGAATTGCGCCGTGCGGCAAGCCAGTACGC
CGACGAGGTGGCGGCCGGGGTGTTCCCGGCCGAGGAGCACAGCTTCTAG

Upstream 100 bases:

>100_bases
GTTCACCTGCGCGCGCCGGAGTGGCAGACTTGGGACGTCAGACGAACCCGGGGACCCGGATGGGCCTCGAGGATCGACCC
GACCATCACGAGGGACAACG

Downstream 100 bases:

>100_bases
GCGGACTGTCTTACGCGAAGTCCGCCCTGCGTTTGGCGAGGAACGCGTCGACACCTTCGCGGCCGTCGGCGGAGTCGGCG
CGGCGCGCAATCGTCCGGCC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSEQTVYGAASDQPTPKPRVKVRTTHLQKWKAEGHKWAMLTAYDFSTARAFDDAGIPVLLVGDSAANVVYGYDTTVPITI
DELIPLVRGVVRGAPHALVVADLPFGSYEEGPRQALATATRFLKETGAHAVKLEGGERVAEQIATLSAAGIPVMAHIGFT
PQSVNGLGGFKVQGRGDAAEQTIHDAIAVQEAGAFSVVMEMVPAELATQITGKLTIPTVGIGAGPNCDAQVLVWQDMAGL
TSGRTAKFVKRFGDVGAELRRAASQYADEVAAGVFPAEEHSF

Sequences:

>Translated_282_residues
MSEQTVYGAASDQPTPKPRVKVRTTHLQKWKAEGHKWAMLTAYDFSTARAFDDAGIPVLLVGDSAANVVYGYDTTVPITI
DELIPLVRGVVRGAPHALVVADLPFGSYEEGPRQALATATRFLKETGAHAVKLEGGERVAEQIATLSAAGIPVMAHIGFT
PQSVNGLGGFKVQGRGDAAEQTIHDAIAVQEAGAFSVVMEMVPAELATQITGKLTIPTVGIGAGPNCDAQVLVWQDMAGL
TSGRTAKFVKRFGDVGAELRRAASQYADEVAAGVFPAEEHSF
>Mature_281_residues
SEQTVYGAASDQPTPKPRVKVRTTHLQKWKAEGHKWAMLTAYDFSTARAFDDAGIPVLLVGDSAANVVYGYDTTVPITID
ELIPLVRGVVRGAPHALVVADLPFGSYEEGPRQALATATRFLKETGAHAVKLEGGERVAEQIATLSAAGIPVMAHIGFTP
QSVNGLGGFKVQGRGDAAEQTIHDAIAVQEAGAFSVVMEMVPAELATQITGKLTIPTVGIGAGPNCDAQVLVWQDMAGLT
SGRTAKFVKRFGDVGAELRRAASQYADEVAAGVFPAEEHSF

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=257, Percent_Identity=47.8599221789883, Blast_Score=234, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6319653, Length=292, Percent_Identity=35.958904109589, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_MYCSJ (A3Q1U4)

Other databases:

- EMBL:   CP000580
- RefSeq:   YP_001071612.1
- ProteinModelPortal:   A3Q1U4
- SMR:   A3Q1U4
- STRING:   A3Q1U4
- EnsemblBacteria:   EBMYCT00000051190
- GeneID:   4879055
- GenomeReviews:   CP000580_GR
- KEGG:   mjl:Mjls_3343
- eggNOG:   COG0413
- GeneTree:   EBGT00050000015555
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- PhylomeDB:   A3Q1U4
- ProtClustDB:   PRK00311
- BioCyc:   MSP164757:MJLS_3343-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29727; Mature: 29596

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: ACT_SITE 200-200 BINDING 102-102 BINDING 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQTVYGAASDQPTPKPRVKVRTTHLQKWKAEGHKWAMLTAYDFSTARAFDDAGIPVLL
CCCCEEEECCCCCCCCCCCEEEEHHHHHHHHCCCCEEEEEEEECCHHHHHHCCCCCEEEE
VGDSAANVVYGYDTTVPITIDELIPLVRGVVRGAPHALVVADLPFGSYEEGPRQALATAT
ECCCCCCEEEECCCEECEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHH
RFLKETGAHAVKLEGGERVAEQIATLSAAGIPVMAHIGFTPQSVNGLGGFKVQGRGDAAE
HHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEECCCCHHH
QTIHDAIAVQEAGAFSVVMEMVPAELATQITGKLTIPTVGIGAGPNCDAQVLVWQDMAGL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEECEEECCCCCCCCEEEEEEECCCCC
TSGRTAKFVKRFGDVGAELRRAASQYADEVAAGVFPAEEHSF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SEQTVYGAASDQPTPKPRVKVRTTHLQKWKAEGHKWAMLTAYDFSTARAFDDAGIPVLL
CCCEEEECCCCCCCCCCCEEEEHHHHHHHHCCCCEEEEEEEECCHHHHHHCCCCCEEEE
VGDSAANVVYGYDTTVPITIDELIPLVRGVVRGAPHALVVADLPFGSYEEGPRQALATAT
ECCCCCCEEEECCCEECEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHH
RFLKETGAHAVKLEGGERVAEQIATLSAAGIPVMAHIGFTPQSVNGLGGFKVQGRGDAAE
HHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEECCCCHHH
QTIHDAIAVQEAGAFSVVMEMVPAELATQITGKLTIPTVGIGAGPNCDAQVLVWQDMAGL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEECEEECCCCCCCCEEEEEEECCCCC
TSGRTAKFVKRFGDVGAELRRAASQYADEVAAGVFPAEEHSF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA