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The map label for this gene is citS [H]

Identifier: 119869332

GI number: 119869332

Start: 3460999

End: 3462618

Strand: Direct

Name: citS [H]

Synonym: Mkms_3300

Alternate gene names: 119869332

Gene position: 3460999-3462618 (Clockwise)

Preceding gene: 119869327

Following gene: 119869333

Centisome position: 60.33

GC content: 70.12

Gene sequence:

>1620_bases
TTGAGTTCACGTTGGTCCGCGACGGTGCGCGGATTCGGTGCCCGCAGCCTGGCCGGACGGTTCCTGGTGTTCCAGCTGCT
GGTGGTGGCGGTGGTGCTGCCGGCCGTGGCGGCGGTCTCGATCGCGCAGTCCACCCGGGAGTTCCGGGAAACGCGCAGCC
AACGCATGATCGCGGTCGCCGAGAACGTCGCGTCCACCCCGATCGTGCGGGACCGCTTGGCCGATCCGTTCGCCGCGCGG
GTACTGGCGCCGGAGGTGGATCGGGCGGTGGCGCTCTCAGGTGCGGCGCTGGCGGAGATCGTCGCTGCGGACGGCACGGT
GCTGGTGTCGTCGGATCCGTCGCGCGTCGGTAACCGGGTGGACCTGAGCGTCACCCGCGCCGACGAGGGCCGGGCCTGGT
ACGGCGACGACGACATCGACGGTGTGCACAGCTTGATCGGGCACGTGCCGGTGCTGACCAACCGGGGTGAGGTGCTGGCG
ATCGCGTCGGTCAGCGAACCGTATCCGTCGGTGTGGCAACTGCTCAGCGGGGCCGGTGAACGTCTCGTGCTCTACCTCGG
ACTGGCCGCGGCACTGGGGCTGGTGGCCTCGTGGCTGCTGTCGCGGCGGATCAAGCGACACACCAGGGGCCTGGAGGTGG
CCGAGATCGCCGGCCTGGCGGATCACCGGGAAGCGTTGCTGCACAGCATCCGTGAGGGTGTCGTGGCCGTGAACACCGAC
GGTGTCATCACCCTGCTCAACGACAGCGCCCAGGAGTTGCTCGGACTCTCGGGGGCGGCGGTGGGCCGGCGCGCCGATGC
GGTGGGGCTCGATCCGGCGGTGGTCGAATTCCTGCTCTCCGGTGAGGACGGCCGCGACGTCGTGATCGCCACCCGCACCC
GGGTGTTGGCGCTCAACCGGCGCACCGCCTCGAGCGGGGGTGAGTGGATCGGTACGGTGACCACGATGCGTGACAGCACC
GAACTGGCCGCCCTGCAGGCACAGTTGTCGTCACACAAGAGCGTCACCGACACGTTGCGCGCGCAGACGCACGAATTCGC
CAACCAGTTGCACACCATCTCGGGGCTGGTTCAGCTGGGCGAATATGACGCGGTGCGGGATCTCATCGGCGAGTTGACGC
GCCGGCGTGCGGAGATCAGCGACGCGGTGACCCGCCGGATCGCCGACCCCGCGGTCGCGGCGCTGCTGATCGCGAAGACC
ACGCTGGCCGCCGAGAGCGGGGTGACGCTGTGGCTCGACCCGGATTCGCATCTCGGCGCATTGGATCCGGCGGCGGCGAC
CGACGTGATCACCCTGCTGGGCAACCTGATCGACAACGCGGTCGACGTATCCGTGGGTTCGGGGGCGGCGCGGGTGAGCG
TGCGCATCGACGACGCCGACGGATTGTCGATCACCGTCGCCGACACCGGACCCGGTGTGCCCGAACATCTCCGGGAAGAG
ATCTTCGCCCGCGGGGTGACCTCGAAACCCGAGTCGCCGGGCGGTCGCGGGATCGGCCTGGCACTGGTCCGGCTGGTCAC
CGCCCAGCACGGCGGCGTGGTGGACGTCTCCGACCGCGCGGACGGTGGCGCAGTGTTCACCGTGCGGTTGCCGCCCAGCC
GGGTGGCAGCGCATGCGTGA

Upstream 100 bases:

>100_bases
CGATCACGGCCAGCGCACGCAGGTTTCGTCTCACGGTGGTCATACTGGACCCGGAAAGTGACGTGAGTCACTCATTCGGA
AGCAAAGGACGTTTTGGTCG

Downstream 100 bases:

>100_bases
CGTGCTGGTGGTCGACGACGATTTCATGGTCGCCGACATCCACCGCCGGTTCGTCGAAAGGGTGGACGGGTTCCGGGCGG
TCGGGGTTGCCCGAACGGGC

Product: signal transduction histidine kinase regulating citrate/malate metabolism

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MSSRWSATVRGFGARSLAGRFLVFQLLVVAVVLPAVAAVSIAQSTREFRETRSQRMIAVAENVASTPIVRDRLADPFAAR
VLAPEVDRAVALSGAALAEIVAADGTVLVSSDPSRVGNRVDLSVTRADEGRAWYGDDDIDGVHSLIGHVPVLTNRGEVLA
IASVSEPYPSVWQLLSGAGERLVLYLGLAAALGLVASWLLSRRIKRHTRGLEVAEIAGLADHREALLHSIREGVVAVNTD
GVITLLNDSAQELLGLSGAAVGRRADAVGLDPAVVEFLLSGEDGRDVVIATRTRVLALNRRTASSGGEWIGTVTTMRDST
ELAALQAQLSSHKSVTDTLRAQTHEFANQLHTISGLVQLGEYDAVRDLIGELTRRRAEISDAVTRRIADPAVAALLIAKT
TLAAESGVTLWLDPDSHLGALDPAAATDVITLLGNLIDNAVDVSVGSGAARVSVRIDDADGLSITVADTGPGVPEHLREE
IFARGVTSKPESPGGRGIGLALVRLVTAQHGGVVDVSDRADGGAVFTVRLPPSRVAAHA

Sequences:

>Translated_539_residues
MSSRWSATVRGFGARSLAGRFLVFQLLVVAVVLPAVAAVSIAQSTREFRETRSQRMIAVAENVASTPIVRDRLADPFAAR
VLAPEVDRAVALSGAALAEIVAADGTVLVSSDPSRVGNRVDLSVTRADEGRAWYGDDDIDGVHSLIGHVPVLTNRGEVLA
IASVSEPYPSVWQLLSGAGERLVLYLGLAAALGLVASWLLSRRIKRHTRGLEVAEIAGLADHREALLHSIREGVVAVNTD
GVITLLNDSAQELLGLSGAAVGRRADAVGLDPAVVEFLLSGEDGRDVVIATRTRVLALNRRTASSGGEWIGTVTTMRDST
ELAALQAQLSSHKSVTDTLRAQTHEFANQLHTISGLVQLGEYDAVRDLIGELTRRRAEISDAVTRRIADPAVAALLIAKT
TLAAESGVTLWLDPDSHLGALDPAAATDVITLLGNLIDNAVDVSVGSGAARVSVRIDDADGLSITVADTGPGVPEHLREE
IFARGVTSKPESPGGRGIGLALVRLVTAQHGGVVDVSDRADGGAVFTVRLPPSRVAAHA
>Mature_538_residues
SSRWSATVRGFGARSLAGRFLVFQLLVVAVVLPAVAAVSIAQSTREFRETRSQRMIAVAENVASTPIVRDRLADPFAARV
LAPEVDRAVALSGAALAEIVAADGTVLVSSDPSRVGNRVDLSVTRADEGRAWYGDDDIDGVHSLIGHVPVLTNRGEVLAI
ASVSEPYPSVWQLLSGAGERLVLYLGLAAALGLVASWLLSRRIKRHTRGLEVAEIAGLADHREALLHSIREGVVAVNTDG
VITLLNDSAQELLGLSGAAVGRRADAVGLDPAVVEFLLSGEDGRDVVIATRTRVLALNRRTASSGGEWIGTVTTMRDSTE
LAALQAQLSSHKSVTDTLRAQTHEFANQLHTISGLVQLGEYDAVRDLIGELTRRRAEISDAVTRRIADPAVAALLIAKTT
LAAESGVTLWLDPDSHLGALDPAAATDVITLLGNLIDNAVDVSVGSGAARVSVRIDDADGLSITVADTGPGVPEHLREEI
FARGVTSKPESPGGRGIGLALVRLVTAQHGGVVDVSDRADGGAVFTVRLPPSRVAAHA

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=445, Percent_Identity=30.7865168539326, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1790567, Length=527, Percent_Identity=29.2220113851992, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1790861, Length=246, Percent_Identity=27.6422764227642, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788549, Length=330, Percent_Identity=25.4545454545455, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1787374, Length=259, Percent_Identity=28.1853281853282, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56486; Mature: 56355

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRWSATVRGFGARSLAGRFLVFQLLVVAVVLPAVAAVSIAQSTREFRETRSQRMIAVA
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENVASTPIVRDRLADPFAARVLAPEVDRAVALSGAALAEIVAADGTVLVSSDPSRVGNRV
HHHHCCCHHHHHCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCEEEEECCHHHCCCEE
DLSVTRADEGRAWYGDDDIDGVHSLIGHVPVLTNRGEVLAIASVSEPYPSVWQLLSGAGE
EEEEEECCCCCEECCCCCHHHHHHHHCCCCEEECCCCEEEEEECCCCCHHHHHHHHCCCC
RLVLYLGLAAALGLVASWLLSRRIKRHTRGLEVAEIAGLADHREALLHSIREGVVAVNTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHCCEEEECCC
GVITLLNDSAQELLGLSGAAVGRRADAVGLDPAVVEFLLSGEDGRDVVIATRTRVLALNR
CEEEEECCCHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECEEEEEEEC
RTASSGGEWIGTVTTMRDSTELAALQAQLSSHKSVTDTLRAQTHEFANQLHTISGLVQLG
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
EYDAVRDLIGELTRRRAEISDAVTRRIADPAVAALLIAKTTLAAESGVTLWLDPDSHLGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
LDPAAATDVITLLGNLIDNAVDVSVGSGAARVSVRIDDADGLSITVADTGPGVPEHLREE
CCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHH
IFARGVTSKPESPGGRGIGLALVRLVTAQHGGVVDVSDRADGGAVFTVRLPPSRVAAHA
HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCHHHHCCCC
>Mature Secondary Structure 
SSRWSATVRGFGARSLAGRFLVFQLLVVAVVLPAVAAVSIAQSTREFRETRSQRMIAVA
CCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENVASTPIVRDRLADPFAARVLAPEVDRAVALSGAALAEIVAADGTVLVSSDPSRVGNRV
HHHHCCCHHHHHCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCEEEEECCHHHCCCEE
DLSVTRADEGRAWYGDDDIDGVHSLIGHVPVLTNRGEVLAIASVSEPYPSVWQLLSGAGE
EEEEEECCCCCEECCCCCHHHHHHHHCCCCEEECCCCEEEEEECCCCCHHHHHHHHCCCC
RLVLYLGLAAALGLVASWLLSRRIKRHTRGLEVAEIAGLADHREALLHSIREGVVAVNTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHCCEEEECCC
GVITLLNDSAQELLGLSGAAVGRRADAVGLDPAVVEFLLSGEDGRDVVIATRTRVLALNR
CEEEEECCCHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECEEEEEEEC
RTASSGGEWIGTVTTMRDSTELAALQAQLSSHKSVTDTLRAQTHEFANQLHTISGLVQLG
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
EYDAVRDLIGELTRRRAEISDAVTRRIADPAVAALLIAKTTLAAESGVTLWLDPDSHLGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
LDPAAATDVITLLGNLIDNAVDVSVGSGAARVSVRIDDADGLSITVADTGPGVPEHLREE
CCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHH
IFARGVTSKPESPGGRGIGLALVRLVTAQHGGVVDVSDRADGGAVFTVRLPPSRVAAHA
HHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]