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The map label for this gene is nasD [H]

Identifier: 119869325

GI number: 119869325

Start: 3453917

End: 3456376

Strand: Direct

Name: nasD [H]

Synonym: Mkms_3293

Alternate gene names: 119869325

Gene position: 3453917-3456376 (Clockwise)

Preceding gene: 119869324

Following gene: 119869326

Centisome position: 60.2

GC content: 68.66

Gene sequence:

>2460_bases
ATGACGCGCAGACGACTCGTCGTCATCGGCAACGGGATGGCCGGCGTCCGCGCGATCGAGGAGATCCTCGCGCGCGGCGG
CGCCGACATGTTCGAGATCACCGTGTTCGGCGACGAACCGTACGGCAACTACAACCGCATCCTGCTGTCGAACGTGTTGT
CCGGCAGTGACGATACCTCCGAGATCTACCTCAACCCCCTCGACTGGTACACCGGCAACGGCATCGATCTGCGGGCCGGG
GTGCGGGTGGTGCGCATCGACCGCTACGCCCGTCTGGTGCACGCCGACGACGGCACCGCACTGCACTACGACGAGCTGAT
CGTGGCCACCGGGAGCCGCTCCTTCTTCCCGCCGATCACCGGGTTGTGGGCCGACGACAAGACGCTGGCCGACGGGGTGT
TCGGCTTCCGCACCCTCGACGACTGCCTGGGCATGATCCACGAGGCCGAACACCGGACCAAGGCCGTGGTCATCGGCGGT
GGGCTGCTGGGGCTGGAAGCCGCACGCGGCCTGCAGAACCGGGGTCTGACCGTGGACGTGGTGCACGCCGGCGCAACGCT
GATGAACGCCCAACTCGACGACACCGCGGGCGCGATCCTGCGACGCTCGGTCGAACGCCTGGGGATCACGGTGCACATGG
ACAAACGCACCACCGAGGCACTCCTCGGCGAGGACGGGAAGCTGCGCGGCATCGGCTTCTCCGACGGCAGCACCGTCGAC
TGCGACATGCTGGTGGTCGCGGCGGGTATCCGGCCCAACGTCGGGCTCGCGCAGCGGGCCGGCCTGACCGTCGAACGGGC
CATCGTCGTCGACGACCACATGCGCTCGATCGACGACGACCACATCCACGTGGTCGGTGAGTGCGCGCAGCACCGCGGCC
AGGTGTACGGGCTGGTGGCGCCGCTGTGGGAGCAGGCAGGGGTGCTGGCCGCCCACCTCACCGGCACCGACCCGACCGCG
GCGTATCACGGCTCCCGGACCGCGACCAAGCTCAAGGTCGCCGGGGTCGACGTCGCCGCGATGGGGCTCAAGGCGCCCGA
ACTGCCCGACGACGAGTTCGTGCAGTACTCCGAGCCCAGGCACGGGGTGTACAAGACCATCGTCATCCGCGACGGAAAGC
TGGTCGGGGCAACACTTCTCGGCGACGTCAGCAAGGTCTCGTTCCTGACGCAGGCGTTCGACACGGGCCTGCCGCTCCCC
GAGGAGCGGGTATCGCTGATGTTCGACATCGGCACCCCCGACGTCGAGGTGGGGGTCGCCGAGCTGGCCGACGACGCGCA
GGTGTGCAACTGCAACGGGGTCTCCAAGGGCACGCTCGTGGGGTGCGTGCGCGACGGGGAGGCCTCGGTGTCGGGGGTGA
TGGCGAAGACCAAGGCGGGCAAGGGGTGCGGCTCGTGCAAGGAACTCGTCGGCCAGATCGTCGAATGGGCCGCCGGCGGG
GCCGTCACCGAGGATCCGTCGGCGTCCTGGTATGTGCCGGGTGTCCCGTACGACAAGCCGACGCTGATGCGCCACATCCG
TGAGCTGCGTCTGCATTCGGTGTCCTCGGTGTTCGCCGCACTCGCCCCCGACGGCAGGGAGGACGCCGGCTCGAAGATGG
CGCTGGCCTCACTGCTCGAGATGATGTGGGCCGACGAGTTCGTCGACGAGCGCGACGCCCGGTTCATCAACGACCGGGTG
CATGCCAACATCCAGCGCGACGGCACCTTCTCGGTGGTTCCGCAGATGAAGGGCGGCGTCACTAACCCGTGGCAGCTGCG
CCGCATCGCCGACGTCGCGGACAAGTACTCGATCCCGATGATCAAGCTCACCGGCGGGCAGCGCATCGACCTGCTCGGGG
TGCGCAAAGAGGATCTGCCCGCGGTGTGGGCCGATCTGGACATGCCGTCCGGCTACGCGTACGGCAAGAGCTTCCGCACG
GTGAAGACGTGCGTGGGCAGCGACTTCTGCCGGTTCGGCGTCGGTGACTCGACGGCGCTGGGGATCGCGATCGAAGACCG
GTACAAGGGGTTGGCCAGTCCGGCGAAGATGAAGCTCGCCGTCACGGGATGTCCGCGCAACTGCGCCGAGTCGCTGTGCA
AGGACCTCGGGGTCGTCGCGATCGACGGCGGCCGCTGGGAGATCTACGTGGGCGGGGCGGCCGGCGCGCACATCCGCAAG
GGCGACCTGCTGGCCACCGTCGACTCTCCCGACGAGGTCATGACGCTGACGGGGCGGTTCCTGCAGTACTACCGGGAGAA
CGCCAACTGGCTGGAGCGCACCTACGCATTCGTCCCACGGGTCGGCATCGAGAAGATCCGCGACGCGATCGCCGAGGACG
CCGAAGGCCTGGATCAACGGATGCAGAAGTCCGTCGACGCCTACCGCGATCCGTGGCACGACGGCCGGGCGCCGGCGACC
GAGGGGCAGTTCCGGTCGTCGCTGCCGCTGCTGCCCCTTCCCCAGGTGCCGGTGCGATGA

Upstream 100 bases:

>100_bases
TGGAGGACCTCCTCGGTGACACGGTGCTCGAACTGTTCGTCGCCGCCCCCGCCGGGTGCGCTGGGACGTTGATCGTCGAT
CTCGGACTGGTGGAGATCTG

Downstream 100 bases:

>100_bases
GCGACGTGGAGCTCGGCGCGGTCGACGAGATCCCCGTCGGTGAGGGCCGCACGTTCACCGTCGACGGTGACCAGATCGCG
GTGTTCCGGCTGCGTGACGG

Product: nitrite reductase (NAD(P)H), large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 819; Mature: 818

Protein sequence:

>819_residues
MTRRRLVVIGNGMAGVRAIEEILARGGADMFEITVFGDEPYGNYNRILLSNVLSGSDDTSEIYLNPLDWYTGNGIDLRAG
VRVVRIDRYARLVHADDGTALHYDELIVATGSRSFFPPITGLWADDKTLADGVFGFRTLDDCLGMIHEAEHRTKAVVIGG
GLLGLEAARGLQNRGLTVDVVHAGATLMNAQLDDTAGAILRRSVERLGITVHMDKRTTEALLGEDGKLRGIGFSDGSTVD
CDMLVVAAGIRPNVGLAQRAGLTVERAIVVDDHMRSIDDDHIHVVGECAQHRGQVYGLVAPLWEQAGVLAAHLTGTDPTA
AYHGSRTATKLKVAGVDVAAMGLKAPELPDDEFVQYSEPRHGVYKTIVIRDGKLVGATLLGDVSKVSFLTQAFDTGLPLP
EERVSLMFDIGTPDVEVGVAELADDAQVCNCNGVSKGTLVGCVRDGEASVSGVMAKTKAGKGCGSCKELVGQIVEWAAGG
AVTEDPSASWYVPGVPYDKPTLMRHIRELRLHSVSSVFAALAPDGREDAGSKMALASLLEMMWADEFVDERDARFINDRV
HANIQRDGTFSVVPQMKGGVTNPWQLRRIADVADKYSIPMIKLTGGQRIDLLGVRKEDLPAVWADLDMPSGYAYGKSFRT
VKTCVGSDFCRFGVGDSTALGIAIEDRYKGLASPAKMKLAVTGCPRNCAESLCKDLGVVAIDGGRWEIYVGGAAGAHIRK
GDLLATVDSPDEVMTLTGRFLQYYRENANWLERTYAFVPRVGIEKIRDAIAEDAEGLDQRMQKSVDAYRDPWHDGRAPAT
EGQFRSSLPLLPLPQVPVR

Sequences:

>Translated_819_residues
MTRRRLVVIGNGMAGVRAIEEILARGGADMFEITVFGDEPYGNYNRILLSNVLSGSDDTSEIYLNPLDWYTGNGIDLRAG
VRVVRIDRYARLVHADDGTALHYDELIVATGSRSFFPPITGLWADDKTLADGVFGFRTLDDCLGMIHEAEHRTKAVVIGG
GLLGLEAARGLQNRGLTVDVVHAGATLMNAQLDDTAGAILRRSVERLGITVHMDKRTTEALLGEDGKLRGIGFSDGSTVD
CDMLVVAAGIRPNVGLAQRAGLTVERAIVVDDHMRSIDDDHIHVVGECAQHRGQVYGLVAPLWEQAGVLAAHLTGTDPTA
AYHGSRTATKLKVAGVDVAAMGLKAPELPDDEFVQYSEPRHGVYKTIVIRDGKLVGATLLGDVSKVSFLTQAFDTGLPLP
EERVSLMFDIGTPDVEVGVAELADDAQVCNCNGVSKGTLVGCVRDGEASVSGVMAKTKAGKGCGSCKELVGQIVEWAAGG
AVTEDPSASWYVPGVPYDKPTLMRHIRELRLHSVSSVFAALAPDGREDAGSKMALASLLEMMWADEFVDERDARFINDRV
HANIQRDGTFSVVPQMKGGVTNPWQLRRIADVADKYSIPMIKLTGGQRIDLLGVRKEDLPAVWADLDMPSGYAYGKSFRT
VKTCVGSDFCRFGVGDSTALGIAIEDRYKGLASPAKMKLAVTGCPRNCAESLCKDLGVVAIDGGRWEIYVGGAAGAHIRK
GDLLATVDSPDEVMTLTGRFLQYYRENANWLERTYAFVPRVGIEKIRDAIAEDAEGLDQRMQKSVDAYRDPWHDGRAPAT
EGQFRSSLPLLPLPQVPVR
>Mature_818_residues
TRRRLVVIGNGMAGVRAIEEILARGGADMFEITVFGDEPYGNYNRILLSNVLSGSDDTSEIYLNPLDWYTGNGIDLRAGV
RVVRIDRYARLVHADDGTALHYDELIVATGSRSFFPPITGLWADDKTLADGVFGFRTLDDCLGMIHEAEHRTKAVVIGGG
LLGLEAARGLQNRGLTVDVVHAGATLMNAQLDDTAGAILRRSVERLGITVHMDKRTTEALLGEDGKLRGIGFSDGSTVDC
DMLVVAAGIRPNVGLAQRAGLTVERAIVVDDHMRSIDDDHIHVVGECAQHRGQVYGLVAPLWEQAGVLAAHLTGTDPTAA
YHGSRTATKLKVAGVDVAAMGLKAPELPDDEFVQYSEPRHGVYKTIVIRDGKLVGATLLGDVSKVSFLTQAFDTGLPLPE
ERVSLMFDIGTPDVEVGVAELADDAQVCNCNGVSKGTLVGCVRDGEASVSGVMAKTKAGKGCGSCKELVGQIVEWAAGGA
VTEDPSASWYVPGVPYDKPTLMRHIRELRLHSVSSVFAALAPDGREDAGSKMALASLLEMMWADEFVDERDARFINDRVH
ANIQRDGTFSVVPQMKGGVTNPWQLRRIADVADKYSIPMIKLTGGQRIDLLGVRKEDLPAVWADLDMPSGYAYGKSFRTV
KTCVGSDFCRFGVGDSTALGIAIEDRYKGLASPAKMKLAVTGCPRNCAESLCKDLGVVAIDGGRWEIYVGGAAGAHIRKG
DLLATVDSPDEVMTLTGRFLQYYRENANWLERTYAFVPRVGIEKIRDAIAEDAEGLDQRMQKSVDAYRDPWHDGRAPATE
GQFRSSLPLLPLPQVPVR

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=257, Percent_Identity=28.0155642023346, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI226437568, Length=257, Percent_Identity=28.0155642023346, Blast_Score=86, Evalue=2e-16,
Organism=Homo sapiens, GI21389617, Length=257, Percent_Identity=28.0155642023346, Blast_Score=86, Evalue=2e-16,
Organism=Escherichia coli, GI1789765, Length=828, Percent_Identity=35.2657004830918, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1789065, Length=358, Percent_Identity=25.6983240223464, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1788892, Length=278, Percent_Identity=24.8201438848921, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=409, Percent_Identity=21.760391198044, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281359715, Length=293, Percent_Identity=24.5733788395904, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI281359713, Length=293, Percent_Identity=24.5733788395904, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24639250, Length=293, Percent_Identity=24.5733788395904, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI18543267, Length=293, Percent_Identity=24.5733788395904, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24639252, Length=293, Percent_Identity=24.5733788395904, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24639257, Length=293, Percent_Identity=24.5733788395904, Blast_Score=94, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24585130, Length=276, Percent_Identity=22.1014492753623, Blast_Score=89, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88407; Mature: 88275

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRRLVVIGNGMAGVRAIEEILARGGADMFEITVFGDEPYGNYNRILLSNVLSGSDDTS
CCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCC
EIYLNPLDWYTGNGIDLRAGVRVVRIDRYARLVHADDGTALHYDELIVATGSRSFFPPIT
EEEEECCCCCCCCCEEECCCEEEEEEEEEEEEEECCCCCEEEECEEEEEECCCCCCCCCC
GLWADDKTLADGVFGFRTLDDCLGMIHEAEHRTKAVVIGGGLLGLEAARGLQNRGLTVDV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCCEEEE
VHAGATLMNAQLDDTAGAILRRSVERLGITVHMDKRTTEALLGEDGKLRGIGFSDGSTVD
EECCCEEEECCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCEEEECCCCCCCCC
CDMLVVAAGIRPNVGLAQRAGLTVERAIVVDDHMRSIDDDHIHVVGECAQHRGQVYGLVA
CEEEEEEECCCCCCCCHHHCCCEEEEEEEEEHHHHCCCCCCEEEEEHHHHCCCCEEEEHH
PLWEQAGVLAAHLTGTDPTAAYHGSRTATKLKVAGVDVAAMGLKAPELPDDEFVQYSEPR
HHHHCCCEEEEEECCCCCCCEECCCCCCEEEEEECCCEEEECCCCCCCCCHHHHCCCCCC
HGVYKTIVIRDGKLVGATLLGDVSKVSFLTQAFDTGLPLPEERVSLMFDIGTPDVEVGVA
CCCEEEEEEECCCEEEHHHHCCHHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCHHHHHH
ELADDAQVCNCNGVSKGTLVGCVRDGEASVSGVMAKTKAGKGCGSCKELVGQIVEWAAGG
HHCCCCCEECCCCCCCCCEEEEEECCCCCHHHHEECCCCCCCCCHHHHHHHHHHHHHCCC
AVTEDPSASWYVPGVPYDKPTLMRHIRELRLHSVSSVFAALAPDGREDAGSKMALASLLE
CCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
MMWADEFVDERDARFINDRVHANIQRDGTFSVVPQMKGGVTNPWQLRRIADVADKYSIPM
HHHHHHHHCHHHHHHHHHHHEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCE
IKLTGGQRIDLLGVRKEDLPAVWADLDMPSGYAYGKSFRTVKTCVGSDFCRFGVGDSTAL
EEECCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCHHCCCCCCCCEE
GIAIEDRYKGLASPAKMKLAVTGCPRNCAESLCKDLGVVAIDGGRWEIYVGGAAGAHIRK
EEEEEHHHCCCCCCCEEEEEEECCCHHHHHHHHHHCCEEEEECCEEEEEECCCCCCEEEC
GDLLATVDSPDEVMTLTGRFLQYYRENANWLERTYAFVPRVGIEKIRDAIAEDAEGLDQR
CCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
MQKSVDAYRDPWHDGRAPATEGQFRSSLPLLPLPQVPVR
HHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TRRRLVVIGNGMAGVRAIEEILARGGADMFEITVFGDEPYGNYNRILLSNVLSGSDDTS
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCC
EIYLNPLDWYTGNGIDLRAGVRVVRIDRYARLVHADDGTALHYDELIVATGSRSFFPPIT
EEEEECCCCCCCCCEEECCCEEEEEEEEEEEEEECCCCCEEEECEEEEEECCCCCCCCCC
GLWADDKTLADGVFGFRTLDDCLGMIHEAEHRTKAVVIGGGLLGLEAARGLQNRGLTVDV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCCEEEE
VHAGATLMNAQLDDTAGAILRRSVERLGITVHMDKRTTEALLGEDGKLRGIGFSDGSTVD
EECCCEEEECCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCEEEECCCCCCCCC
CDMLVVAAGIRPNVGLAQRAGLTVERAIVVDDHMRSIDDDHIHVVGECAQHRGQVYGLVA
CEEEEEEECCCCCCCCHHHCCCEEEEEEEEEHHHHCCCCCCEEEEEHHHHCCCCEEEEHH
PLWEQAGVLAAHLTGTDPTAAYHGSRTATKLKVAGVDVAAMGLKAPELPDDEFVQYSEPR
HHHHCCCEEEEEECCCCCCCEECCCCCCEEEEEECCCEEEECCCCCCCCCHHHHCCCCCC
HGVYKTIVIRDGKLVGATLLGDVSKVSFLTQAFDTGLPLPEERVSLMFDIGTPDVEVGVA
CCCEEEEEEECCCEEEHHHHCCHHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCHHHHHH
ELADDAQVCNCNGVSKGTLVGCVRDGEASVSGVMAKTKAGKGCGSCKELVGQIVEWAAGG
HHCCCCCEECCCCCCCCCEEEEEECCCCCHHHHEECCCCCCCCCHHHHHHHHHHHHHCCC
AVTEDPSASWYVPGVPYDKPTLMRHIRELRLHSVSSVFAALAPDGREDAGSKMALASLLE
CCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
MMWADEFVDERDARFINDRVHANIQRDGTFSVVPQMKGGVTNPWQLRRIADVADKYSIPM
HHHHHHHHCHHHHHHHHHHHEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCE
IKLTGGQRIDLLGVRKEDLPAVWADLDMPSGYAYGKSFRTVKTCVGSDFCRFGVGDSTAL
EEECCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCHHCCCCCCCCEE
GIAIEDRYKGLASPAKMKLAVTGCPRNCAESLCKDLGVVAIDGGRWEIYVGGAAGAHIRK
EEEEEHHHCCCCCCCEEEEEEECCCHHHHHHHHHHCCEEEEECCEEEEEECCCCCCEEEC
GDLLATVDSPDEVMTLTGRFLQYYRENANWLERTYAFVPRVGIEKIRDAIAEDAEGLDQR
CCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
MQKSVDAYRDPWHDGRAPATEGQFRSSLPLLPLPQVPVR
HHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]