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The map label for this gene is camA [H]

Identifier: 119869313

GI number: 119869313

Start: 3439290

End: 3440489

Strand: Direct

Name: camA [H]

Synonym: Mkms_3281

Alternate gene names: 119869313

Gene position: 3439290-3440489 (Clockwise)

Preceding gene: 119869312

Following gene: 119869318

Centisome position: 59.95

GC content: 63.5

Gene sequence:

>1200_bases
GTGACTCCCGAACGAGCGGTGATCGTCGGCGCGAGCCACGCCGGCGCGCAGTTGGCAGCTAATCTTCGAAGGGAGGGGTG
GTCTGGGGAGGTCGTGCTCATCGGCGACGAGGGGGGACTGCCCTACCACCGGCCTCCGTTGTCGAAGGGATACCTGGCCG
GCAAGAACGGCCTCGACGACCTCCTGATTCGCGGCGCTGATTTCTACGAAAAGCAGCACATTCGACTCTTGAATGCGACC
GTGGAGGCGATCCACCGGAGTGCCAAGCGTGTGTCTCTGAGCACCGGCGACACGCTGACGTACACCAAGCTCGCGTTGTG
CACCGGCGCAAGGGCCAGACGACTCCCCACACCAGGGGTGGATCTTCCCGGAATTCACTACCTGCGTACCGCTGCAGACG
TCGAGTTGATCCGTGCCGCCGCTACACCGGGTCGGAGGGTTGTGATCGTGGGCGGCGGTTACATCGGGTTGGAAACGGCG
GCCTCGCTGTGTTCGCTCGGCATGAACGTCACCGTCCTCGAGGCAACCGAGCGTGTACTCGAACGGGTCACCGCGCCGGA
GGTTTCCGCGTTTTACACCCGAATCCACAACGGCGAGGGAGTGGAGATCCGAACACACGCTCTCGTCGAAGCCTTCTCCG
GCAACGGCAGAGTGCAGGAGGTCGTGCTGGCAGGCGGCGAACCGATCCCCGCCGACTTGGTCATCGTCGGCGTCGGTGTG
GTGCCGAACACCGAGCTCGCCTCGGCCGCAGGATTATCCGTCGACAACGGCATCGTGATCGACGACCAGGCCCGCACCAG
CGACCCCGACATCGTGGCCGCCGGCGACTGCACCAGCCACACCATGGCTCGATACGGCTCGCGTATTCGTTTGGAATCCG
TATCGAGCGCCGGCGAGCAGGCCAAGATCGCTGCGGCGACAATCTGCGGAAAACACAGCGCAATCGCTGCGCTTCCGTGG
TTCTGGTCCGACCAGTACGATCTCAAACTCCAGATCGCCGGTCTCAACACCGGATACGACGAAGTGGTCTTCAGCGGCGA
CCCGTCGCGTGACCGTGACTTCAGCTGCTTCTACTTCCGCGATCGTGAACTCATCGCCGCCGACTGCGTCAACCGTCCTC
GCGATTTCATGTTCAGTAAGCGGGCCATCAGCCAGCAGCTCCGAGTTGACCGCTCAGAGCTCCTCGCCGGCTCGATCTGA

Upstream 100 bases:

>100_bases
TTGAGAACATCGAGGTGGTCGGCGAACCCGAGTATGTGCAATCGAATTTCGTCAGGGGTATCAGCAAGCTGATGGTTCGC
CTCACTCCCAGGCCAAGTGC

Downstream 100 bases:

>100_bases
CACGAGAGCTTGCTAGTTACAAACTGTGGACAACTCCCAGGTACCTGAGTGCTACGCGGGAGATCCGCTCACCGGTGGTG
GCGATGTCCGCGGCTGGGAG

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTPERAVIVGASHAGAQLAANLRREGWSGEVVLIGDEGGLPYHRPPLSKGYLAGKNGLDDLLIRGADFYEKQHIRLLNAT
VEAIHRSAKRVSLSTGDTLTYTKLALCTGARARRLPTPGVDLPGIHYLRTAADVELIRAAATPGRRVVIVGGGYIGLETA
ASLCSLGMNVTVLEATERVLERVTAPEVSAFYTRIHNGEGVEIRTHALVEAFSGNGRVQEVVLAGGEPIPADLVIVGVGV
VPNTELASAAGLSVDNGIVIDDQARTSDPDIVAAGDCTSHTMARYGSRIRLESVSSAGEQAKIAAATICGKHSAIAALPW
FWSDQYDLKLQIAGLNTGYDEVVFSGDPSRDRDFSCFYFRDRELIAADCVNRPRDFMFSKRAISQQLRVDRSELLAGSI

Sequences:

>Translated_399_residues
MTPERAVIVGASHAGAQLAANLRREGWSGEVVLIGDEGGLPYHRPPLSKGYLAGKNGLDDLLIRGADFYEKQHIRLLNAT
VEAIHRSAKRVSLSTGDTLTYTKLALCTGARARRLPTPGVDLPGIHYLRTAADVELIRAAATPGRRVVIVGGGYIGLETA
ASLCSLGMNVTVLEATERVLERVTAPEVSAFYTRIHNGEGVEIRTHALVEAFSGNGRVQEVVLAGGEPIPADLVIVGVGV
VPNTELASAAGLSVDNGIVIDDQARTSDPDIVAAGDCTSHTMARYGSRIRLESVSSAGEQAKIAAATICGKHSAIAALPW
FWSDQYDLKLQIAGLNTGYDEVVFSGDPSRDRDFSCFYFRDRELIAADCVNRPRDFMFSKRAISQQLRVDRSELLAGSI
>Mature_398_residues
TPERAVIVGASHAGAQLAANLRREGWSGEVVLIGDEGGLPYHRPPLSKGYLAGKNGLDDLLIRGADFYEKQHIRLLNATV
EAIHRSAKRVSLSTGDTLTYTKLALCTGARARRLPTPGVDLPGIHYLRTAADVELIRAAATPGRRVVIVGGGYIGLETAA
SLCSLGMNVTVLEATERVLERVTAPEVSAFYTRIHNGEGVEIRTHALVEAFSGNGRVQEVVLAGGEPIPADLVIVGVGVV
PNTELASAAGLSVDNGIVIDDQARTSDPDIVAAGDCTSHTMARYGSRIRLESVSSAGEQAKIAAATICGKHSAIAALPWF
WSDQYDLKLQIAGLNTGYDEVVFSGDPSRDRDFSCFYFRDRELIAADCVNRPRDFMFSKRAISQQLRVDRSELLAGSI

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI65787454, Length=371, Percent_Identity=29.9191374663073, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI21389617, Length=371, Percent_Identity=29.9191374663073, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI226437568, Length=371, Percent_Identity=29.9191374663073, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI4757732, Length=333, Percent_Identity=24.3243243243243, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI22202629, Length=333, Percent_Identity=24.3243243243243, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788892, Length=402, Percent_Identity=36.0696517412935, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1789065, Length=272, Percent_Identity=31.25, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789765, Length=345, Percent_Identity=27.8260869565217, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559934, Length=364, Percent_Identity=31.043956043956, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI32564386, Length=409, Percent_Identity=24.9388753056235, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24639257, Length=367, Percent_Identity=29.1553133514986, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI281359715, Length=367, Percent_Identity=29.1553133514986, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI281359713, Length=367, Percent_Identity=29.1553133514986, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24639250, Length=367, Percent_Identity=29.1553133514986, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI18543267, Length=367, Percent_Identity=29.1553133514986, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24639252, Length=367, Percent_Identity=29.1553133514986, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24585130, Length=367, Percent_Identity=24.2506811989101, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24581020, Length=356, Percent_Identity=23.876404494382, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28573993, Length=356, Percent_Identity=23.876404494382, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42708; Mature: 42577

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPERAVIVGASHAGAQLAANLRREGWSGEVVLIGDEGGLPYHRPPLSKGYLAGKNGLDD
CCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEECCCCCHHH
LLIRGADFYEKQHIRLLNATVEAIHRSAKRVSLSTGDTLTYTKLALCTGARARRLPTPGV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHCCCHHCCCCCCCC
DLPGIHYLRTAADVELIRAAATPGRRVVIVGGGYIGLETAASLCSLGMNVTVLEATERVL
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECHHHHHHHHHCCCCEEEEHHHHHHH
ERVTAPEVSAFYTRIHNGEGVEIRTHALVEAFSGNGRVQEVVLAGGEPIPADLVIVGVGV
HHHCCCCHHHHHHHHCCCCCCEEEHHEEHHHHCCCCCEEEEEEECCCCCCCCEEEEEECC
VPNTELASAAGLSVDNGIVIDDQARTSDPDIVAAGDCTSHTMARYGSRIRLESVSSAGEQ
CCCCCHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCHHHHHHCCCEEEEEHHHCCCCC
AKIAAATICGKHSAIAALPWFWSDQYDLKLQIAGLNTGYDEVVFSGDPSRDRDFSCFYFR
CEEEEEECCCCCCCEEEECEEECCCEEEEEEEEECCCCCCCEEECCCCCCCCCEEEEEEE
DRELIAADCVNRPRDFMFSKRAISQQLRVDRSELLAGSI
CCCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHCCCC
>Mature Secondary Structure 
TPERAVIVGASHAGAQLAANLRREGWSGEVVLIGDEGGLPYHRPPLSKGYLAGKNGLDD
CCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEECCCCCHHH
LLIRGADFYEKQHIRLLNATVEAIHRSAKRVSLSTGDTLTYTKLALCTGARARRLPTPGV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHCCCHHCCCCCCCC
DLPGIHYLRTAADVELIRAAATPGRRVVIVGGGYIGLETAASLCSLGMNVTVLEATERVL
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECHHHHHHHHHCCCCEEEEHHHHHHH
ERVTAPEVSAFYTRIHNGEGVEIRTHALVEAFSGNGRVQEVVLAGGEPIPADLVIVGVGV
HHHCCCCHHHHHHHHCCCCCCEEEHHEEHHHHCCCCCEEEEEEECCCCCCCCEEEEEECC
VPNTELASAAGLSVDNGIVIDDQARTSDPDIVAAGDCTSHTMARYGSRIRLESVSSAGEQ
CCCCCHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCHHHHHHCCCEEEEEHHHCCCCC
AKIAAATICGKHSAIAALPWFWSDQYDLKLQIAGLNTGYDEVVFSGDPSRDRDFSCFYFR
CEEEEEECCCCCCCEEEECEEECCCEEEEEEEEECCCCCCCEEECCCCCCCCCEEEEEEE
DRELIAADCVNRPRDFMFSKRAISQQLRVDRSELLAGSI
CCCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]