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The map label for this gene is glgX [H]

Identifier: 119869172

GI number: 119869172

Start: 3283752

End: 3285914

Strand: Direct

Name: glgX [H]

Synonym: Mkms_3140

Alternate gene names: 119869172

Gene position: 3283752-3285914 (Clockwise)

Preceding gene: 119869171

Following gene: 119869173

Centisome position: 57.24

GC content: 65.83

Gene sequence:

>2163_bases
GTGGTCACCCCCGGATCCGGGCCCACTTCACCGACGCTGCACACCGTCTGGCCCGGTGAGGCCTATCCGCTGGGCGCGAC
GTACGACGGGGCGGGCACCAACTTCTCGCTGTTCTCCGAGGTCGCCGAACGCGTCGAGCTGTGCCTGATCGCCAAGGACG
GCAGCGAGGAACGGATCAACCTCGACGAGGTCGACGGCTACGTCTGGCACGCCTACCTGCCCACGGTCACCCCCGGCCAA
CGCTACGGGTTCCGCGTGCACGGTCCGTGGGATCCGTCGGCGGGACACCGGTGCGATCCGAGCAAGCTGCTGCTCGATCC
GTACGGCAAATCGTTCTACGGCGACTTCCGGTTCGGGCAGGCGCTGTACTCCTACGACCTCGACGCCGAGGACCTCGCCT
CGGGCGGTGACCCCCCACGGATCGACTCGCGGGGGCACACGATGACCAGCGTCGTCATCAACCCGTTCTTCCAGTGGGGC
TCCGACCGCGCACCGCGCACGCCGTACCACGACACCGTGATCTACGAGGCCCACGTCAAGGGGATGACCCGGCGGCACCC
GGCCGTGCCCGCGGAGTTACGCGGCACCTACGCCGGGCTCTGCCATCCGGCGATCATCGACCACCTCAAGTCGCTCAACG
TCACCGCCATCGAACTGATGCCGGTGCACCAGTTCATGCACGACCACCGCCTGCTCGACCTCGGATTGCGAAACTACTGG
GGCTACAACACGTTCGGCTTCTTCGCACCGCACAACGAGTACGCCGCCAACCAGCACGCCGGCGGCGCGGTCGCCGAGTT
CAAGGCGATGGTGCGGTCCTTCCACGCGGCGGGTATCGAGGTCATCCTCGACGTCGTCTACAACCACACCGCCGAGGGCA
ACCACCTCGGGCCGACCGTCAACTTCCGCGGTATCGACAACGCCGCCTACTACCGGCTGCTCGACGGTGACCTGCGCCTC
TACAAGGACTTCACCGGGACCGGCAACAGCCTCAACGCCCGCCACCCCCACACCCTGCAGCTCATCATGGACTCCCTGCG
GTACTGGGTGCTGGAGATGCACGTCGACGGGTTCCGCTTCGACCTGGCCTCGACGCTGGCCCGCGAGTTCTACGACGTCG
ACCGACTCTCGGCCTTCTTCGACCTGGTGCAGCAGGATCCGGTGGTCAGTCAGGTCAAGTTGATCGCCGAACCGTGGGAC
ATCGGCGAAGGCGGTTATCAGGTCGGCAACTTCCCAGGTTTGTGGACCGAGTGGAACGGGCAATATCGCGACACTGTGCG
TGACTACTGGCGGGGAGAACCCGCAACCCTCGGCGAATTCGCCTCCCGACTGACGGGATCATCAGACCTCTACGAGGCCA
CCGGACGGCGTCCCAGCGCCTCCATCAACTTCGTGACCTGTCACGACGGGTTCACGATGCACGATCTGGTGTCCTACAAC
GAGAAACACAACGAAGCCAACGGCGAGGACAACCGCGACGGCGAGAGCCACAACCGGTCCTGGAACTGCGGGGTGGAAGG
TCCCACCGACGATCCGGACGTCATCGCGTTGCGCGGTAAGCAGATGCGCAACATGCTGGCCACGCTGATGTGCAGCCAGG
GCACCCCGATGATCGCCCACGGTGACGAGATCGGCCGCACCCAGCAGGGCAACAACAACGTCTACTGCCAGGATTCGGAA
CTGTCCTGGATGGACTGGTCGTTGTGCGAGACCAACGCCGATCTGCTGGAGTTCACCCGCAAGGTGGTCGCCTTCCGCAA
GAACCACCCGGTGTTCCGGCGGCGACGGTTCTTCGAGGGCAAACCGATCCGCAGCGCGGATCAGGTCCGTGACATCGCCT
GGCTGACCCCCGCGGGCACCGAGATGACCCCCGAGGACTGGGGCACCGGCCTGGGCAAGTGCGTGGCGGTCTTCCTCAAC
GGTGAGGCGATCCCGGCCCCCAACGCGCGCGGCGAGCGGGTGGTCGACGATTCGTTCCTGTTGTGCTTCAACGCCCACGA
CCACGTGCAGGACTTCGTCACCCCCGACGGCGACTACGCCGAGATGTGGACCGCCGATCTGGACACCGCCGATCCGACCG
GGCACACCGACGTCGTCGTCCCGGCAGGCGAGAAGATCTCGCTGCAACCCAGATCGCTGCTCGTGCTGCGGAAGACGGCG
TGA

Upstream 100 bases:

>100_bases
TGGTCTATCACGACGCCCACCACATCTCGACCACCTACATGCGCACCATGACCGCCGAACTGGGACGTCAGATCGCCGCC
GCCACCGGCTGGTGGTGACC

Downstream 100 bases:

>100_bases
TGGCCGCCGACCGGCCCGTGCTGTCGACCTACCGGTTGCAGATGCGCGGAGACGCGTTCACCCTCGCCGACGCCGAGGCG
CTGGTGGACTACCTCGACGA

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MVTPGSGPTSPTLHTVWPGEAYPLGATYDGAGTNFSLFSEVAERVELCLIAKDGSEERINLDEVDGYVWHAYLPTVTPGQ
RYGFRVHGPWDPSAGHRCDPSKLLLDPYGKSFYGDFRFGQALYSYDLDAEDLASGGDPPRIDSRGHTMTSVVINPFFQWG
SDRAPRTPYHDTVIYEAHVKGMTRRHPAVPAELRGTYAGLCHPAIIDHLKSLNVTAIELMPVHQFMHDHRLLDLGLRNYW
GYNTFGFFAPHNEYAANQHAGGAVAEFKAMVRSFHAAGIEVILDVVYNHTAEGNHLGPTVNFRGIDNAAYYRLLDGDLRL
YKDFTGTGNSLNARHPHTLQLIMDSLRYWVLEMHVDGFRFDLASTLAREFYDVDRLSAFFDLVQQDPVVSQVKLIAEPWD
IGEGGYQVGNFPGLWTEWNGQYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASINFVTCHDGFTMHDLVSYN
EKHNEANGEDNRDGESHNRSWNCGVEGPTDDPDVIALRGKQMRNMLATLMCSQGTPMIAHGDEIGRTQQGNNNVYCQDSE
LSWMDWSLCETNADLLEFTRKVVAFRKNHPVFRRRRFFEGKPIRSADQVRDIAWLTPAGTEMTPEDWGTGLGKCVAVFLN
GEAIPAPNARGERVVDDSFLLCFNAHDHVQDFVTPDGDYAEMWTADLDTADPTGHTDVVVPAGEKISLQPRSLLVLRKTA

Sequences:

>Translated_720_residues
MVTPGSGPTSPTLHTVWPGEAYPLGATYDGAGTNFSLFSEVAERVELCLIAKDGSEERINLDEVDGYVWHAYLPTVTPGQ
RYGFRVHGPWDPSAGHRCDPSKLLLDPYGKSFYGDFRFGQALYSYDLDAEDLASGGDPPRIDSRGHTMTSVVINPFFQWG
SDRAPRTPYHDTVIYEAHVKGMTRRHPAVPAELRGTYAGLCHPAIIDHLKSLNVTAIELMPVHQFMHDHRLLDLGLRNYW
GYNTFGFFAPHNEYAANQHAGGAVAEFKAMVRSFHAAGIEVILDVVYNHTAEGNHLGPTVNFRGIDNAAYYRLLDGDLRL
YKDFTGTGNSLNARHPHTLQLIMDSLRYWVLEMHVDGFRFDLASTLAREFYDVDRLSAFFDLVQQDPVVSQVKLIAEPWD
IGEGGYQVGNFPGLWTEWNGQYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASINFVTCHDGFTMHDLVSYN
EKHNEANGEDNRDGESHNRSWNCGVEGPTDDPDVIALRGKQMRNMLATLMCSQGTPMIAHGDEIGRTQQGNNNVYCQDSE
LSWMDWSLCETNADLLEFTRKVVAFRKNHPVFRRRRFFEGKPIRSADQVRDIAWLTPAGTEMTPEDWGTGLGKCVAVFLN
GEAIPAPNARGERVVDDSFLLCFNAHDHVQDFVTPDGDYAEMWTADLDTADPTGHTDVVVPAGEKISLQPRSLLVLRKTA
>Mature_720_residues
MVTPGSGPTSPTLHTVWPGEAYPLGATYDGAGTNFSLFSEVAERVELCLIAKDGSEERINLDEVDGYVWHAYLPTVTPGQ
RYGFRVHGPWDPSAGHRCDPSKLLLDPYGKSFYGDFRFGQALYSYDLDAEDLASGGDPPRIDSRGHTMTSVVINPFFQWG
SDRAPRTPYHDTVIYEAHVKGMTRRHPAVPAELRGTYAGLCHPAIIDHLKSLNVTAIELMPVHQFMHDHRLLDLGLRNYW
GYNTFGFFAPHNEYAANQHAGGAVAEFKAMVRSFHAAGIEVILDVVYNHTAEGNHLGPTVNFRGIDNAAYYRLLDGDLRL
YKDFTGTGNSLNARHPHTLQLIMDSLRYWVLEMHVDGFRFDLASTLAREFYDVDRLSAFFDLVQQDPVVSQVKLIAEPWD
IGEGGYQVGNFPGLWTEWNGQYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASINFVTCHDGFTMHDLVSYN
EKHNEANGEDNRDGESHNRSWNCGVEGPTDDPDVIALRGKQMRNMLATLMCSQGTPMIAHGDEIGRTQQGNNNVYCQDSE
LSWMDWSLCETNADLLEFTRKVVAFRKNHPVFRRRRFFEGKPIRSADQVRDIAWLTPAGTEMTPEDWGTGLGKCVAVFLN
GEAIPAPNARGERVVDDSFLLCFNAHDHVQDFVTPDGDYAEMWTADLDTADPTGHTDVVVPAGEKISLQPRSLLVLRKTA

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=662, Percent_Identity=44.4108761329305, Blast_Score=558, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 80567; Mature: 80567

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTPGSGPTSPTLHTVWPGEAYPLGATYDGAGTNFSLFSEVAERVELCLIAKDGSEERIN
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCCCC
LDEVDGYVWHAYLPTVTPGQRYGFRVHGPWDPSAGHRCDPSKLLLDPYGKSFYGDFRFGQ
HHHCCCEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHH
ALYSYDLDAEDLASGGDPPRIDSRGHTMTSVVINPFFQWGSDRAPRTPYHDTVIYEAHVK
HHHHCCCCHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHC
GMTRRHPAVPAELRGTYAGLCHPAIIDHLKSLNVTAIELMPVHQFMHDHRLLDLGLRNYW
CCHHCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHCC
GYNTFGFFAPHNEYAANQHAGGAVAEFKAMVRSFHAAGIEVILDVVYNHTAEGNHLGPTV
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
NFRGIDNAAYYRLLDGDLRLYKDFTGTGNSLNARHPHTLQLIMDSLRYWVLEMHVDGFRF
EEECCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCHHH
DLASTLAREFYDVDRLSAFFDLVQQDPVVSQVKLIAEPWDIGEGGYQVGNFPGLWTEWNG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCC
QYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASINFVTCHDGFTMHDLVSYN
CHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHH
EKHNEANGEDNRDGESHNRSWNCGVEGPTDDPDVIALRGKQMRNMLATLMCSQGTPMIAH
HHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEC
GDEIGRTQQGNNNVYCQDSELSWMDWSLCETNADLLEFTRKVVAFRKNHPVFRRRRFFEG
CHHHCCCCCCCCCEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCC
KPIRSADQVRDIAWLTPAGTEMTPEDWGTGLGKCVAVFLNGEAIPAPNARGERVVDDSFL
CCCCCHHHHHHEEEECCCCCCCCHHHHCCCHHHEEEEEECCCCCCCCCCCCCEEECCCEE
LCFNAHDHVQDFVTPDGDYAEMWTADLDTADPTGHTDVVVPAGEKISLQPRSLLVLRKTA
EEECCCHHHHHHCCCCCCHHHHEECCCCCCCCCCCCEEEEECCCEEEECCCEEEEEEECC
>Mature Secondary Structure
MVTPGSGPTSPTLHTVWPGEAYPLGATYDGAGTNFSLFSEVAERVELCLIAKDGSEERIN
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCCCC
LDEVDGYVWHAYLPTVTPGQRYGFRVHGPWDPSAGHRCDPSKLLLDPYGKSFYGDFRFGQ
HHHCCCEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHH
ALYSYDLDAEDLASGGDPPRIDSRGHTMTSVVINPFFQWGSDRAPRTPYHDTVIYEAHVK
HHHHCCCCHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHC
GMTRRHPAVPAELRGTYAGLCHPAIIDHLKSLNVTAIELMPVHQFMHDHRLLDLGLRNYW
CCHHCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHCC
GYNTFGFFAPHNEYAANQHAGGAVAEFKAMVRSFHAAGIEVILDVVYNHTAEGNHLGPTV
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
NFRGIDNAAYYRLLDGDLRLYKDFTGTGNSLNARHPHTLQLIMDSLRYWVLEMHVDGFRF
EEECCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCHHH
DLASTLAREFYDVDRLSAFFDLVQQDPVVSQVKLIAEPWDIGEGGYQVGNFPGLWTEWNG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCC
QYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASINFVTCHDGFTMHDLVSYN
CHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHH
EKHNEANGEDNRDGESHNRSWNCGVEGPTDDPDVIALRGKQMRNMLATLMCSQGTPMIAH
HHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEC
GDEIGRTQQGNNNVYCQDSELSWMDWSLCETNADLLEFTRKVVAFRKNHPVFRRRRFFEG
CHHHCCCCCCCCCEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCC
KPIRSADQVRDIAWLTPAGTEMTPEDWGTGLGKCVAVFLNGEAIPAPNARGERVVDDSFL
CCCCCHHHHHHEEEECCCCCCCCHHHHCCCHHHEEEEEECCCCCCCCCCCCCEEECCCEE
LCFNAHDHVQDFVTPDGDYAEMWTADLDTADPTGHTDVVVPAGEKISLQPRSLLVLRKTA
EEECCCHHHHHHCCCCCCHHHHEECCCCCCCCCCCCEEEEECCCEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]