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The map label for this gene is braE [H]

Identifier: 119869096

GI number: 119869096

Start: 3206705

End: 3207985

Strand: Reverse

Name: braE [H]

Synonym: Mkms_3064

Alternate gene names: 119869096

Gene position: 3207985-3206705 (Counterclockwise)

Preceding gene: 119869097

Following gene: 119869095

Centisome position: 55.92

GC content: 67.06

Gene sequence:

>1281_bases
ATGAGCGGTCAACGGGCGAACGTGTGGCAGGCGCTCGTCATGCGCGTGCGGGCCGTGTGGGCGGTCCTCGTCGAGCGCAC
GCGACCGGTGTGGGAACGGTTGCGTCCGGTGTGGAGGTGGGTCCTTCGCACCCGTGACCGCCTCCGCGAGTGGTGGGCGA
ACCTGACGCGGGTCCAGAAGTGGGTGGTCGGCGCCGTCGTCTTCCTGGCGATCGGGCTCCTGCCCATCTTCACGCCGTCA
TGGCTGGACACCCCCGGTATCAGCTTCGGCGGCACCATGGCTCAGTTCGCGATGGTCGCGATCATCGCGATCGGCCTCAA
TGTCGTTGTGGGACAAGCTGGTCTACTCGATCTCGGTTATGTCGGCTTCTACGCGGTCGGCGCCTACACCGTCGCTCTGC
TGACCAGTCCCAGCAGCCCGTGGAACCAGGCCGGTCCCGGCGGGTGGTTCACCACCGACTGGGCGTGGCTGGCGTGTGTG
CCGCTGGCGATGGCCGTGACGGCGCTCGCCGGACTCATCCTGGGCACGCCGACACTGCGGTTGCGCGGTGACTACCTGGC
GATCGTCACCCTCGGATTCGGGGAGATCATCCGCCTGCTCGCCGACAACCTCTCCGATGTCACCAACGGGCCCCGCGGCC
TCAACGAGGTCGAGTACCCCCGGGTGGGGGAGAGTGAGCGGCTCCCCGAAGGCGTGTTCTCCCGGGCCAACTCGACGGGG
GAGGCGAACTACGGCACCTGGTGGTTCTGGCTGGGCCTGCTGCTCATCGTCGGGATCCTGCTGCTGGTCGGCAACCTCGA
ACGCAGCAGGGTCGGCCGGGCGTGGGTGGCGGTGCGTGAGGACGAGGACGCCGCGGAGGTCATGGGGGTCAACACCTTCC
GCTTCAAACTCTGGGCGTTCGTGATCGGCGCCGCGATCGGCGGGCTGTCCGGGGCGCTGTACGCCGGCCAGGTCCAGTAC
GTGGCGCCTCCGACGTTCAACATCATCAACTCGATGCTGTTCCTGTGTGCGGTCGTCCTCGGCGGGCAGGGCAACAAGCT
GGGCGTGATCTTCGGTGCATTCATCATCGTTTACCTGCCGAATCGCCTTCTCGGCGTTCACTTCCTGGGCATCAACCTCG
GCGACCTGAAGTATCTGTTCTTCGGCCTGGCCCTGGTGGTGTTGATGATCTTCCGCCCGCAGGGGCTGTTCCCGGCGCGT
CAGCAACTGCTCACCTACGGCAGGGCCGCACGTGACATGTTGCGCCGCGCGCCCGGCGAGAACGAGGGGGCGATGCGATG
A

Upstream 100 bases:

>100_bases
GCACCCAGTGGCGTGACGTGGTCGCCTTCGTCCTGCTGGTTCTGGTCCTGCTCTTCCGCCCCACCGGCATACTCGGTGAG
AGCCTCGGGAAGGCGCGGGT

Downstream 100 bases:

>100_bases
CGATCGAGGACCTCGCCGGTGTGCACCGGGAGATCGTGGCGGCCGAGGGGGAGACGCTGCTGCAGACCCACGACCTCACC
GTGAAGTTCGGCGGGTTGAC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSGQRANVWQALVMRVRAVWAVLVERTRPVWERLRPVWRWVLRTRDRLREWWANLTRVQKWVVGAVVFLAIGLLPIFTPS
WLDTPGISFGGTMAQFAMVAIIAIGLNVVVGQAGLLDLGYVGFYAVGAYTVALLTSPSSPWNQAGPGGWFTTDWAWLACV
PLAMAVTALAGLILGTPTLRLRGDYLAIVTLGFGEIIRLLADNLSDVTNGPRGLNEVEYPRVGESERLPEGVFSRANSTG
EANYGTWWFWLGLLLIVGILLLVGNLERSRVGRAWVAVREDEDAAEVMGVNTFRFKLWAFVIGAAIGGLSGALYAGQVQY
VAPPTFNIINSMLFLCAVVLGGQGNKLGVIFGAFIIVYLPNRLLGVHFLGINLGDLKYLFFGLALVVLMIFRPQGLFPAR
QQLLTYGRAARDMLRRAPGENEGAMR

Sequences:

>Translated_426_residues
MSGQRANVWQALVMRVRAVWAVLVERTRPVWERLRPVWRWVLRTRDRLREWWANLTRVQKWVVGAVVFLAIGLLPIFTPS
WLDTPGISFGGTMAQFAMVAIIAIGLNVVVGQAGLLDLGYVGFYAVGAYTVALLTSPSSPWNQAGPGGWFTTDWAWLACV
PLAMAVTALAGLILGTPTLRLRGDYLAIVTLGFGEIIRLLADNLSDVTNGPRGLNEVEYPRVGESERLPEGVFSRANSTG
EANYGTWWFWLGLLLIVGILLLVGNLERSRVGRAWVAVREDEDAAEVMGVNTFRFKLWAFVIGAAIGGLSGALYAGQVQY
VAPPTFNIINSMLFLCAVVLGGQGNKLGVIFGAFIIVYLPNRLLGVHFLGINLGDLKYLFFGLALVVLMIFRPQGLFPAR
QQLLTYGRAARDMLRRAPGENEGAMR
>Mature_425_residues
SGQRANVWQALVMRVRAVWAVLVERTRPVWERLRPVWRWVLRTRDRLREWWANLTRVQKWVVGAVVFLAIGLLPIFTPSW
LDTPGISFGGTMAQFAMVAIIAIGLNVVVGQAGLLDLGYVGFYAVGAYTVALLTSPSSPWNQAGPGGWFTTDWAWLACVP
LAMAVTALAGLILGTPTLRLRGDYLAIVTLGFGEIIRLLADNLSDVTNGPRGLNEVEYPRVGESERLPEGVFSRANSTGE
ANYGTWWFWLGLLLIVGILLLVGNLERSRVGRAWVAVREDEDAAEVMGVNTFRFKLWAFVIGAAIGGLSGALYAGQVQYV
APPTFNIINSMLFLCAVVLGGQGNKLGVIFGAFIIVYLPNRLLGVHFLGINLGDLKYLFFGLALVVLMIFRPQGLFPARQ
QLLTYGRAARDMLRRAPGENEGAMR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=337, Percent_Identity=40.6528189910979, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 46824; Mature: 46692

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQRANVWQALVMRVRAVWAVLVERTRPVWERLRPVWRWVLRTRDRLREWWANLTRVQK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVVGAVVFLAIGLLPIFTPSWLDTPGISFGGTMAQFAMVAIIAIGLNVVVGQAGLLDLGY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VGFYAVGAYTVALLTSPSSPWNQAGPGGWFTTDWAWLACVPLAMAVTALAGLILGTPTLR
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LRGDYLAIVTLGFGEIIRLLADNLSDVTNGPRGLNEVEYPRVGESERLPEGVFSRANSTG
ECCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
EANYGTWWFWLGLLLIVGILLLVGNLERSRVGRAWVAVREDEDAAEVMGVNTFRFKLWAF
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHHH
VIGAAIGGLSGALYAGQVQYVAPPTFNIINSMLFLCAVVLGGQGNKLGVIFGAFIIVYLP
HHHHHHHHHCCCCEECEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
NRLLGVHFLGINLGDLKYLFFGLALVVLMIFRPQGLFPARQQLLTYGRAARDMLRRAPGE
CHHHHHEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
NEGAMR
CCCCCC
>Mature Secondary Structure 
SGQRANVWQALVMRVRAVWAVLVERTRPVWERLRPVWRWVLRTRDRLREWWANLTRVQK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVVGAVVFLAIGLLPIFTPSWLDTPGISFGGTMAQFAMVAIIAIGLNVVVGQAGLLDLGY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VGFYAVGAYTVALLTSPSSPWNQAGPGGWFTTDWAWLACVPLAMAVTALAGLILGTPTLR
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LRGDYLAIVTLGFGEIIRLLADNLSDVTNGPRGLNEVEYPRVGESERLPEGVFSRANSTG
ECCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
EANYGTWWFWLGLLLIVGILLLVGNLERSRVGRAWVAVREDEDAAEVMGVNTFRFKLWAF
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHHH
VIGAAIGGLSGALYAGQVQYVAPPTFNIINSMLFLCAVVLGGQGNKLGVIFGAFIIVYLP
HHHHHHHHHCCCCEECEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
NRLLGVHFLGINLGDLKYLFFGLALVVLMIFRPQGLFPARQQLLTYGRAARDMLRRAPGE
CHHHHHEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
NEGAMR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]