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The map label for this gene is nylA [H]

Identifier: 119868781

GI number: 119868781

Start: 2874471

End: 2875940

Strand: Direct

Name: nylA [H]

Synonym: Mkms_2749

Alternate gene names: 119868781

Gene position: 2874471-2875940 (Clockwise)

Preceding gene: 119868780

Following gene: 119868782

Centisome position: 50.1

GC content: 73.2

Gene sequence:

>1470_bases
ATGGATGCTGTGGACAACAACTCGGCTTTCGACCTGGCCGGAGTCGACGCGATCGGACAGGCGCAGCTCATCGCCTCCGG
TGAGCTGACGGCCACCGAACTCCTCGACGCCGCGCTCATCCGGCTCTCGGCGGCCCGCGGCCTCAACGCCGTCGTCCACG
AACTGTTCGACCGGGGCCGTGAGCAGGCGGCCGCACTGGACGCCTCGGGACGGCTGCGCAGCGGGGACGCCGGCCCCGTC
GCGGGCGTGCCGTTCCTGCTCAAGGATCTCGGCGCGGCTGTCGGGGGTGCCCCGGAGTCCATGGGGTCACGCGCGCTGCG
CACCCGCATCGCCCCTCAGAACGCGTGGATCGTCGACCGCTACCTGGCCAACGACCTGGTCGTGTTCGGCAAGACCAACA
CCCCGGAGTGGGGCAACCACTGCACGACCGAACCGTCGCTGTTCGGACCGACCGTCAACCCCTGGTCGCCGGACGTGACG
CCGGGCGGGTCCAGCGGTGGCTCGGCCGCCGCGGTGGCCGCCGGTGTGGTGCCCGCCGCGTCGGGCGGTGACGGCACCGG
GTCGATCCGGGTGCCCGCATCGTGCTGCGGGCTGGTCGGGCTCAAACCGCGCCGGGCCCGCACGTCGTTCGCACCGGCCG
GCCACCTGCTCGAGGGTCTTGCCGTCGAGCATGCGCTGACGCGGACGGTGCGTGACAGCGCGGCCCTTCTCGACGCCGTC
ACCGGGTCCGACCCCGGCGACCCGTACAACGCGGCACCGCCCGCGATGCCGTTCCTGCAGGCACTCGCCCAGCCACCGGC
TCCGCAGCGCATCGCGTTGTCGACCGGTTCACCGTTCCCCGGCCCCGCCACCGACCCCGAGATCGTCGCGGCGGTCGAGG
CCACCGCCCGCCTGCTGGCCGACCTCGGCCACCGGGTCGAACCCGCCGCCACCGCGGTGAACGCCGACGCCGTCGCCGAC
GCGATCGCCGTGCTGCACAACGTCAGCAACGCCCAACTGCACGAACTGGCCTCCGCACACCTCGGCCGCCCGCCCGCCGA
CGACGAATTCGAGGCCAGCACCTGGGTGATGGTGCGTGAGGGGTTCACGACGACCGGGGTGCAGTACGCCGGGGCGATCG
AAACCGTGCACGCTGAGACCCGCCGGTTCGTCACTGGGATGGCCGGCCACGACGTGCTGTTGGCGCCGACCCTGCTGACT
CCCCCACCCCCGTACGCCCTGCTCGACCAGCCCCGCGGCACCACCCGGGCGTTCTTCGACGTCGAGTTCGCGACGACCGG
GTGGACGTCGCTGGCCAACGTCACCGGTTGGGCGGCGATCTCACTGCCGCTGGGCACCACCTCGACCGGCCTGCCGATCG
GGGTCCAGCTGATGGCGCCGGAGGAGACCGTGCTGCTGCAACTCGCGGCGCAACTGGAGGTGGCCGCGCCGTGGGCGGAC
CGCCGCCCGGCCGGGTGGGTCGCGCCGTGA

Upstream 100 bases:

>100_bases
CGCGATCCAGAGCATCTACGAGGCGTCGATCGCCGGCTGACCGTCCCCTGCGGGGCGTCGGGTTGCCAGTCGGCCCGGCG
CACTGCAGGATCGCCGAGCT

Downstream 100 bases:

>100_bases
TCGGGAGCCGTCCGGTCCGCCATCGGTGACATGCGACACTGCTGGGCATGACGCCGCCCATCGCCCGCCCGAAGCTCGAA
GGCAATATCGCGGTCACCGC

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MDAVDNNSAFDLAGVDAIGQAQLIASGELTATELLDAALIRLSAARGLNAVVHELFDRGREQAAALDASGRLRSGDAGPV
AGVPFLLKDLGAAVGGAPESMGSRALRTRIAPQNAWIVDRYLANDLVVFGKTNTPEWGNHCTTEPSLFGPTVNPWSPDVT
PGGSSGGSAAAVAAGVVPAASGGDGTGSIRVPASCCGLVGLKPRRARTSFAPAGHLLEGLAVEHALTRTVRDSAALLDAV
TGSDPGDPYNAAPPAMPFLQALAQPPAPQRIALSTGSPFPGPATDPEIVAAVEATARLLADLGHRVEPAATAVNADAVAD
AIAVLHNVSNAQLHELASAHLGRPPADDEFEASTWVMVREGFTTTGVQYAGAIETVHAETRRFVTGMAGHDVLLAPTLLT
PPPPYALLDQPRGTTRAFFDVEFATTGWTSLANVTGWAAISLPLGTTSTGLPIGVQLMAPEETVLLQLAAQLEVAAPWAD
RRPAGWVAP

Sequences:

>Translated_489_residues
MDAVDNNSAFDLAGVDAIGQAQLIASGELTATELLDAALIRLSAARGLNAVVHELFDRGREQAAALDASGRLRSGDAGPV
AGVPFLLKDLGAAVGGAPESMGSRALRTRIAPQNAWIVDRYLANDLVVFGKTNTPEWGNHCTTEPSLFGPTVNPWSPDVT
PGGSSGGSAAAVAAGVVPAASGGDGTGSIRVPASCCGLVGLKPRRARTSFAPAGHLLEGLAVEHALTRTVRDSAALLDAV
TGSDPGDPYNAAPPAMPFLQALAQPPAPQRIALSTGSPFPGPATDPEIVAAVEATARLLADLGHRVEPAATAVNADAVAD
AIAVLHNVSNAQLHELASAHLGRPPADDEFEASTWVMVREGFTTTGVQYAGAIETVHAETRRFVTGMAGHDVLLAPTLLT
PPPPYALLDQPRGTTRAFFDVEFATTGWTSLANVTGWAAISLPLGTTSTGLPIGVQLMAPEETVLLQLAAQLEVAAPWAD
RRPAGWVAP
>Mature_489_residues
MDAVDNNSAFDLAGVDAIGQAQLIASGELTATELLDAALIRLSAARGLNAVVHELFDRGREQAAALDASGRLRSGDAGPV
AGVPFLLKDLGAAVGGAPESMGSRALRTRIAPQNAWIVDRYLANDLVVFGKTNTPEWGNHCTTEPSLFGPTVNPWSPDVT
PGGSSGGSAAAVAAGVVPAASGGDGTGSIRVPASCCGLVGLKPRRARTSFAPAGHLLEGLAVEHALTRTVRDSAALLDAV
TGSDPGDPYNAAPPAMPFLQALAQPPAPQRIALSTGSPFPGPATDPEIVAAVEATARLLADLGHRVEPAATAVNADAVAD
AIAVLHNVSNAQLHELASAHLGRPPADDEFEASTWVMVREGFTTTGVQYAGAIETVHAETRRFVTGMAGHDVLLAPTLLT
PPPPYALLDQPRGTTRAFFDVEFATTGWTSLANVTGWAAISLPLGTTSTGLPIGVQLMAPEETVLLQLAAQLEVAAPWAD
RRPAGWVAP

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=27.0750988142292, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=343, Percent_Identity=29.7376093294461, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=480, Percent_Identity=25.4166666666667, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=501, Percent_Identity=23.5528942115768, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=512, Percent_Identity=23.4375, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=449, Percent_Identity=25.8351893095768, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24644968, Length=501, Percent_Identity=24.5508982035928, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648113, Length=499, Percent_Identity=27.8557114228457, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=24.4047619047619, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356731, Length=196, Percent_Identity=33.6734693877551, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI45550774, Length=212, Percent_Identity=30.6603773584906, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648435, Length=212, Percent_Identity=30.6603773584906, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648437, Length=212, Percent_Identity=30.6603773584906, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 50164; Mature: 50164

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVDNNSAFDLAGVDAIGQAQLIASGELTATELLDAALIRLSAARGLNAVVHELFDRGR
CCCCCCCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCH
EQAAALDASGRLRSGDAGPVAGVPFLLKDLGAAVGGAPESMGSRALRTRIAPQNAWIVDR
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEHH
YLANDLVVFGKTNTPEWGNHCTTEPSLFGPTVNPWSPDVTPGGSSGGSAAAVAAGVVPAA
HHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCC
SGGDGTGSIRVPASCCGLVGLKPRRARTSFAPAGHLLEGLAVEHALTRTVRDSAALLDAV
CCCCCCCEEECCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSDPGDPYNAAPPAMPFLQALAQPPAPQRIALSTGSPFPGPATDPEIVAAVEATARLLA
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
DLGHRVEPAATAVNADAVADAIAVLHNVSNAQLHELASAHLGRPPADDEFEASTWVMVRE
HHCCCCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEC
GFTTTGVQYAGAIETVHAETRRFVTGMAGHDVLLAPTLLTPPPPYALLDQPRGTTRAFFD
CCCCCCEEHHCHHHHHHHHHHHHHHCCCCCCEEEECEECCCCCCHHHCCCCCCCCEEEEE
VEFATTGWTSLANVTGWAAISLPLGTTSTGLPIGVQLMAPEETVLLQLAAQLEVAAPWAD
EEECCCCCHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHEECCCCC
RRPAGWVAP
CCCCCCCCC
>Mature Secondary Structure
MDAVDNNSAFDLAGVDAIGQAQLIASGELTATELLDAALIRLSAARGLNAVVHELFDRGR
CCCCCCCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCH
EQAAALDASGRLRSGDAGPVAGVPFLLKDLGAAVGGAPESMGSRALRTRIAPQNAWIVDR
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEHH
YLANDLVVFGKTNTPEWGNHCTTEPSLFGPTVNPWSPDVTPGGSSGGSAAAVAAGVVPAA
HHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCC
SGGDGTGSIRVPASCCGLVGLKPRRARTSFAPAGHLLEGLAVEHALTRTVRDSAALLDAV
CCCCCCCEEECCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSDPGDPYNAAPPAMPFLQALAQPPAPQRIALSTGSPFPGPATDPEIVAAVEATARLLA
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
DLGHRVEPAATAVNADAVADAIAVLHNVSNAQLHELASAHLGRPPADDEFEASTWVMVRE
HHCCCCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEC
GFTTTGVQYAGAIETVHAETRRFVTGMAGHDVLLAPTLLTPPPPYALLDQPRGTTRAFFD
CCCCCCEEHHCHHHHHHHHHHHHHHCCCCCCEEEECEECCCCCCHHHCCCCCCCCEEEEE
VEFATTGWTSLANVTGWAAISLPLGTTSTGLPIGVQLMAPEETVLLQLAAQLEVAAPWAD
EEECCCCCHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHEECCCCC
RRPAGWVAP
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]