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The map label for this gene is bphC [H]

Identifier: 119868689

GI number: 119868689

Start: 2787521

End: 2788390

Strand: Reverse

Name: bphC [H]

Synonym: Mkms_2656

Alternate gene names: 119868689

Gene position: 2788390-2787521 (Counterclockwise)

Preceding gene: 119868690

Following gene: 119868688

Centisome position: 48.6

GC content: 64.14

Gene sequence:

>870_bases
ATGGCCTCCGTTTCACCGCCCGACATCGTCCGCTGTGCGTACATGGAATTGGTCGTCACCGACCTGGAACGATCACGGGA
TTTCTACGTCGACATCCTGGGTCTGGTCGTCACCGAGGAAACCTCTGACGCAATCTATCTGCGGGCGTTCGAGGAGTTCA
TCCACCACAATCTGGTGCTGCGCAGGGGCCCGGTCGCCGCGGTGGCTGCGTTCGCCTTCCGGGTCCGCAGCCCCGAGGAT
GTCGACCGTGCCGAGGCCTACTACAAGTCGCTCGGCGTGCGCACCGAGCGGCGCGCGAGCGGATTCACCCGCGGAGTCGG
AGACAGCGTCCGGGTGGAGGACCCGTTGGGTTTCCCCTACGAGTTCTTCTACGACGTCGTGCATGTCGAGCGCATGGCCT
GGCGCTACGACGTCCAGGGGGCGGGCGCCCTGGTACGCCTCGACCACTTCAATCAGGTGATCCCTGATGTGCCGCGAGGC
CGGAAATACCTCGAAGAGTTGGGTTTTCGCGCCACTGAGGACATCCGTGACGATGCAGGCATCACCTACGCCGCGTGGTT
CCGGCGCAAAGACACCGTCCACGACACCGCGCTGACCGGCGGCGACGGTCCCAGAATGCACCACATCGCCTTCGCCACCC
ACGAGAAACACAACATCCTCTACATCTGCGACAAGCTCGGTGCGCTGCGTCGATCCGACATCATCGAACGCGGTCCCGGC
CGGCACGGGGTGTCCAACGCGTTCTACCTCTACCTACGCGACCCCGACGGACATCGGGTGGAGATCTACACGCAGGACTA
CTACACCGGCGACCCGGACAACCCGCTGGTGAGCTGGGACGTGCACGACAACCAGCGACGGGACTGGTAG

Upstream 100 bases:

>100_bases
CCAGCAGGCTGTGCACATCACCCTCGGCGACGTCCACACACCGAGATTCGGCATCACCGCCCGCTGACCAGACTCCCTTC
TGATGAAAGTGAGCCGACAC

Downstream 100 bases:

>100_bases
GGCCACGCCGTGGTTCCGAGCTGGTACACCGAGGCCTCGCTGGTGCTCGACCTCGACGGTGTCCCGCAGCCCGTCCAGGT
GCGCACCGACCAGAGCGAGA

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Products: NA

Alternate protein names: Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase; Mn(II)-dependent 23OHBP oxygenase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MASVSPPDIVRCAYMELVVTDLERSRDFYVDILGLVVTEETSDAIYLRAFEEFIHHNLVLRRGPVAAVAAFAFRVRSPED
VDRAEAYYKSLGVRTERRASGFTRGVGDSVRVEDPLGFPYEFFYDVVHVERMAWRYDVQGAGALVRLDHFNQVIPDVPRG
RKYLEELGFRATEDIRDDAGITYAAWFRRKDTVHDTALTGGDGPRMHHIAFATHEKHNILYICDKLGALRRSDIIERGPG
RHGVSNAFYLYLRDPDGHRVEIYTQDYYTGDPDNPLVSWDVHDNQRRDW

Sequences:

>Translated_289_residues
MASVSPPDIVRCAYMELVVTDLERSRDFYVDILGLVVTEETSDAIYLRAFEEFIHHNLVLRRGPVAAVAAFAFRVRSPED
VDRAEAYYKSLGVRTERRASGFTRGVGDSVRVEDPLGFPYEFFYDVVHVERMAWRYDVQGAGALVRLDHFNQVIPDVPRG
RKYLEELGFRATEDIRDDAGITYAAWFRRKDTVHDTALTGGDGPRMHHIAFATHEKHNILYICDKLGALRRSDIIERGPG
RHGVSNAFYLYLRDPDGHRVEIYTQDYYTGDPDNPLVSWDVHDNQRRDW
>Mature_288_residues
ASVSPPDIVRCAYMELVVTDLERSRDFYVDILGLVVTEETSDAIYLRAFEEFIHHNLVLRRGPVAAVAAFAFRVRSPEDV
DRAEAYYKSLGVRTERRASGFTRGVGDSVRVEDPLGFPYEFFYDVVHVERMAWRYDVQGAGALVRLDHFNQVIPDVPRGR
KYLEELGFRATEDIRDDAGITYAAWFRRKDTVHDTALTGGDGPRMHHIAFATHEKHNILYICDKLGALRRSDIIERGPGR
HGVSNAFYLYLRDPDGHRVEIYTQDYYTGDPDNPLVSWDVHDNQRRDW

Specific function: Catalyzes the meta-cleavage of the hydroxylated biphenyl ring. The enzyme can oxidize a wide range of substrates, and the substrate preference order is 2,3-dihydroxybiphenyl > 3- methylcatechol > catechol > 4-methylcatechol > 4-chlorocatechol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the extradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =1.13.11.39 [H]

Molecular weight: Translated: 33258; Mature: 33126

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00082 EXTRADIOL_DIOXYGENAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVSPPDIVRCAYMELVVTDLERSRDFYVDILGLVVTEETSDAIYLRAFEEFIHHNLVL
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCCCEEHHHHHHHHHHHCEEE
RRGPVAAVAAFAFRVRSPEDVDRAEAYYKSLGVRTERRASGFTRGVGDSVRVEDPLGFPY
ECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEECCCCCCCH
EFFYDVVHVERMAWRYDVQGAGALVRLDHFNQVIPDVPRGRKYLEELGFRATEDIRDDAG
HHHHHHHHHHHHHHEECCCCCCEEEEEHHHHHHCCCCCCHHHHHHHCCCCCCHHHHCCCC
ITYAAWFRRKDTVHDTALTGGDGPRMHHIAFATHEKHNILYICDKLGALRRSDIIERGPG
CEEEHHHCCCCCCCHHEEECCCCCCEEEEEEEECCCCCEEEEECHHCHHHHHHHHHCCCC
RHGVSNAFYLYLRDPDGHRVEIYTQDYYTGDPDNPLVSWDVHDNQRRDW
CCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
ASVSPPDIVRCAYMELVVTDLERSRDFYVDILGLVVTEETSDAIYLRAFEEFIHHNLVL
CCCCCCHHHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCCCEEHHHHHHHHHHHCEEE
RRGPVAAVAAFAFRVRSPEDVDRAEAYYKSLGVRTERRASGFTRGVGDSVRVEDPLGFPY
ECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEECCCCCCCH
EFFYDVVHVERMAWRYDVQGAGALVRLDHFNQVIPDVPRGRKYLEELGFRATEDIRDDAG
HHHHHHHHHHHHHHEECCCCCCEEEEEHHHHHHCCCCCCHHHHHHHCCCCCCHHHHCCCC
ITYAAWFRRKDTVHDTALTGGDGPRMHHIAFATHEKHNILYICDKLGALRRSDIIERGPG
CEEEHHHCCCCCCCHHEEECCCCCCEEEEEEEECCCCCEEEEECHHCHHHHHHHHHCCCC
RHGVSNAFYLYLRDPDGHRVEIYTQDYYTGDPDNPLVSWDVHDNQRRDW
CCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12672826 [H]