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The map label for this gene is paaI [H]

Identifier: 119868685

GI number: 119868685

Start: 2785213

End: 2785659

Strand: Reverse

Name: paaI [H]

Synonym: Mkms_2652

Alternate gene names: 119868685

Gene position: 2785659-2785213 (Counterclockwise)

Preceding gene: 119868688

Following gene: 119868678

Centisome position: 48.55

GC content: 64.88

Gene sequence:

>447_bases
ATGGACGAGACGATGCCTGATCTTCTCACCGACGACCGCATGTCCGCGCATCTCGGTATCCAGGTGAAGCATCGGGAGCC
GGGACACGCGGTGGTCACCATGACCGTCACGTCCGGCATGGCGAACGGTCACGGCATCACCCACGGTGGTGCTGTCTTTG
CGCTGGCCGACACGGCGTTCGCGCTCGCCTGCAACCGCAGCGCCGGGGTGATGGGGGTGACCGTCAGTGCGACCATCGAC
TTCATCGCTGCGAGTGGTGTCGGTGACGTTCTGGTGGCCCGTGCGTGCGAGCGCGTGGAGCGGGGACGAAGCGGTCTCTA
CGACGTCACGGTGTATCGCGGTGACGAGGTGATCGCGGAGTTTCGCGCTCGTAGCCGGACCGTGGCTGCTCCAAGTAGTC
GGGTCACTGCGGTGGGTCATCGGGAATCGTCTTCCAGGCAAAGCTGA

Upstream 100 bases:

>100_bases
ACCGTCATGGATGCTGACCCCGTTGAAGACGCGAAAACCCGGGGATATTCAGTGGTTTTCGCTGCTTACCCAAGGCGCCT
GGGTAGGAGAGGATGAAGCC

Downstream 100 bases:

>100_bases
ACCCACGCAGCACTGCGGCGACCGCTCCGTCATCCAACGGTCCGTCGCCCGACCGTTCGATCACCGCGGTGGGAATCCCG
TCGACCGCGACCCCGGAAAC

Product: hypothetical protein

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MDETMPDLLTDDRMSAHLGIQVKHREPGHAVVTMTVTSGMANGHGITHGGAVFALADTAFALACNRSAGVMGVTVSATID
FIAASGVGDVLVARACERVERGRSGLYDVTVYRGDEVIAEFRARSRTVAAPSSRVTAVGHRESSSRQS

Sequences:

>Translated_148_residues
MDETMPDLLTDDRMSAHLGIQVKHREPGHAVVTMTVTSGMANGHGITHGGAVFALADTAFALACNRSAGVMGVTVSATID
FIAASGVGDVLVARACERVERGRSGLYDVTVYRGDEVIAEFRARSRTVAAPSSRVTAVGHRESSSRQS
>Mature_148_residues
MDETMPDLLTDDRMSAHLGIQVKHREPGHAVVTMTVTSGMANGHGITHGGAVFALADTAFALACNRSAGVMGVTVSATID
FIAASGVGDVLVARACERVERGRSGLYDVTVYRGDEVIAEFRARSRTVAAPSSRVTAVGHRESSSRQS

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=122, Percent_Identity=42.6229508196721, Blast_Score=91, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 15545; Mature: 15545

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDETMPDLLTDDRMSAHLGIQVKHREPGHAVVTMTVTSGMANGHGITHGGAVFALADTAF
CCCCCCCHHCCCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCEE
ALACNRSAGVMGVTVSATIDFIAASGVGDVLVARACERVERGRSGLYDVTVYRGDEVIAE
EEEECCCCCEEEEEEEHEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHH
FRARSRTVAAPSSRVTAVGHRESSSRQS
HHHCCCEEECCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MDETMPDLLTDDRMSAHLGIQVKHREPGHAVVTMTVTSGMANGHGITHGGAVFALADTAF
CCCCCCCHHCCCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCEE
ALACNRSAGVMGVTVSATIDFIAASGVGDVLVARACERVERGRSGLYDVTVYRGDEVIAE
EEEECCCCCEEEEEEEHEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHH
FRARSRTVAAPSSRVTAVGHRESSSRQS
HHHCCCEEECCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]