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The map label for this gene is potD-A [H]

Identifier: 119868646

GI number: 119868646

Start: 2745282

End: 2746472

Strand: Reverse

Name: potD-A [H]

Synonym: Mkms_2611

Alternate gene names: 119868646

Gene position: 2746472-2745282 (Counterclockwise)

Preceding gene: 119868647

Following gene: 119868645

Centisome position: 47.87

GC content: 67.17

Gene sequence:

>1191_bases
ATGCCCCGTTCGTCAGAGTTCGACCCGCAACTGGCCGCCCGCCTCGCCGCCACCCGGGCGTCACGGCGCCGGTTCCTCGG
CGGCGGCGCCGCCGCGGCCGCCGCGCTGGCGTTGGGCCCGTCGGTCCTGGCCGCCTGCGGTTCGGACAGCGGCGGCTCCG
AGGCGACGACCACCGACGACGGTGGCCCCGCCGGTGGGACGTTGCGGGTCTCGAACTGGCCGCTCTACATGGCCGACGGC
TTCGTCGCCGCCTTCCAGACCGCGTCCGGGATCACCGTCGACTACAAAGAGGACTTCAACGACAACGAGGAATGGTTCGC
CAAGGTCAAGGAGCCGCTGTCGCGTAAGCAGGACATCGGCGCCGATCTGGTCATCCCGACCGAGTTCATGGCGGCCCGCA
TCAACGGACTCGGGTGGCTCAACGAGATCCGTGCCGACCGGGTGCCCAACAAGAAGAACCTGCGTCCCGACCTGTTGAAC
TCCGCCATCGACCCGGGCCGCAACAAGACCGCGCCGTACATGACGGGCATGGTGGGCCTCGCCTACAACAAGGCCGCCAC
CGGACGTGAGATCACCCGGATCGAGGACCTGTGGGATCCGGCGTTCAAGGGCCGGGTCAGCCTGCTCTCGGACACCCAGG
ACGGTCTGGGCATGCTGATGCTCTCGCAGGGCGCCTCCCCCGAGCGGCCGTCCACCGAGGCGGTGCAGAAGGCCGTCGAC
CTGGTGCGTGAGCAGAAGGACAAGGGGCAGATCCGCCGCTTCACCGGCAACGACTACGCCGACGACCTCGCAGCGGGCAA
TGTCGCCATCGCACAGGCGTATTCGGGTGATGTGGTGCAGCTGAAGGCGGACAACCCCGACCTGGAGTTCATCGTTCCCC
AGTCCGGTGGCACGTGGTTCATCGACACGATGGTCATCCCCTACACCACCCAGAACCAGGCCGCTGCCGAGGCGTGGATC
GACTACGTCTACGACCGCGCCAACTACGCGAAGCTGATCGCCTTCACCCAGTTCGTGCCGGTGCTGTCGGAGATGACCGA
CGAGCTCAACAAGATCGATCCCGGCGTGGCGGCCAACCCGCTGATCAACCCGCCGGAGTCGACGGTGAACAACCTCAAGT
CGTGGGCGGCGCTGACCGATGAGCAGACGCAGGAGTACAACACCATGTACGCCGCAGTGACCGGGGGCTGA

Upstream 100 bases:

>100_bases
TCTCGAAGAGATGCTCGACGAGTCCTGACCCTCGTACCGTCCCGCCGTTTCCCTCCCCGGCCCTTCTCACCTCGAGAAGA
TCACGAAAGGCACACCCCTC

Downstream 100 bases:

>100_bases
CGCCCACCCATGGCAGGTGTAGCCAGCAGTAGCCGGCAGCGCAGCAGGATCGCTCCCTATCTGATGATCCTGCCCGCGCT
GGCTTATCTCGCAATCTTTT

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MPRSSEFDPQLAARLAATRASRRRFLGGGAAAAAALALGPSVLAACGSDSGGSEATTTDDGGPAGGTLRVSNWPLYMADG
FVAAFQTASGITVDYKEDFNDNEEWFAKVKEPLSRKQDIGADLVIPTEFMAARINGLGWLNEIRADRVPNKKNLRPDLLN
SAIDPGRNKTAPYMTGMVGLAYNKAATGREITRIEDLWDPAFKGRVSLLSDTQDGLGMLMLSQGASPERPSTEAVQKAVD
LVREQKDKGQIRRFTGNDYADDLAAGNVAIAQAYSGDVVQLKADNPDLEFIVPQSGGTWFIDTMVIPYTTQNQAAAEAWI
DYVYDRANYAKLIAFTQFVPVLSEMTDELNKIDPGVAANPLINPPESTVNNLKSWAALTDEQTQEYNTMYAAVTGG

Sequences:

>Translated_396_residues
MPRSSEFDPQLAARLAATRASRRRFLGGGAAAAAALALGPSVLAACGSDSGGSEATTTDDGGPAGGTLRVSNWPLYMADG
FVAAFQTASGITVDYKEDFNDNEEWFAKVKEPLSRKQDIGADLVIPTEFMAARINGLGWLNEIRADRVPNKKNLRPDLLN
SAIDPGRNKTAPYMTGMVGLAYNKAATGREITRIEDLWDPAFKGRVSLLSDTQDGLGMLMLSQGASPERPSTEAVQKAVD
LVREQKDKGQIRRFTGNDYADDLAAGNVAIAQAYSGDVVQLKADNPDLEFIVPQSGGTWFIDTMVIPYTTQNQAAAEAWI
DYVYDRANYAKLIAFTQFVPVLSEMTDELNKIDPGVAANPLINPPESTVNNLKSWAALTDEQTQEYNTMYAAVTGG
>Mature_395_residues
PRSSEFDPQLAARLAATRASRRRFLGGGAAAAAALALGPSVLAACGSDSGGSEATTTDDGGPAGGTLRVSNWPLYMADGF
VAAFQTASGITVDYKEDFNDNEEWFAKVKEPLSRKQDIGADLVIPTEFMAARINGLGWLNEIRADRVPNKKNLRPDLLNS
AIDPGRNKTAPYMTGMVGLAYNKAATGREITRIEDLWDPAFKGRVSLLSDTQDGLGMLMLSQGASPERPSTEAVQKAVDL
VREQKDKGQIRRFTGNDYADDLAAGNVAIAQAYSGDVVQLKADNPDLEFIVPQSGGTWFIDTMVIPYTTQNQAAAEAWID
YVYDRANYAKLIAFTQFVPVLSEMTDELNKIDPGVAANPLINPPESTVNNLKSWAALTDEQTQEYNTMYAAVTGG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=306, Percent_Identity=25.4901960784314, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787078, Length=281, Percent_Identity=28.8256227758007, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 42675; Mature: 42544

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSSEFDPQLAARLAATRASRRRFLGGGAAAAAALALGPSVLAACGSDSGGSEATTTDD
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCC
GGPAGGTLRVSNWPLYMADGFVAAFQTASGITVDYKEDFNDNEEWFAKVKEPLSRKQDIG
CCCCCCEEEECCCCEEEECCHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCC
ADLVIPTEFMAARINGLGWLNEIRADRVPNKKNLRPDLLNSAIDPGRNKTAPYMTGMVGL
CCEEECHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHH
AYNKAATGREITRIEDLWDPAFKGRVSLLSDTQDGLGMLMLSQGASPERPSTEAVQKAVD
HCCCCCCCCHHHHHHHHCCHHHHCCEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
LVREQKDKGQIRRFTGNDYADDLAAGNVAIAQAYSGDVVQLKADNPDLEFIVPQSGGTWF
HHHHHHCCCCEEECCCCCHHHHHHCCCEEEEEECCCCEEEEECCCCCEEEEEECCCCCEE
IDTMVIPYTTQNQAAAEAWIDYVYDRANYAKLIAFTQFVPVLSEMTDELNKIDPGVAANP
EEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LINPPESTVNNLKSWAALTDEQTQEYNTMYAAVTGG
CCCCCHHHHHHHHHHHHHCCHHHHHHCEEEEEEECC
>Mature Secondary Structure 
PRSSEFDPQLAARLAATRASRRRFLGGGAAAAAALALGPSVLAACGSDSGGSEATTTDD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCC
GGPAGGTLRVSNWPLYMADGFVAAFQTASGITVDYKEDFNDNEEWFAKVKEPLSRKQDIG
CCCCCCEEEECCCCEEEECCHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCC
ADLVIPTEFMAARINGLGWLNEIRADRVPNKKNLRPDLLNSAIDPGRNKTAPYMTGMVGL
CCEEECHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHH
AYNKAATGREITRIEDLWDPAFKGRVSLLSDTQDGLGMLMLSQGASPERPSTEAVQKAVD
HCCCCCCCCHHHHHHHHCCHHHHCCEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
LVREQKDKGQIRRFTGNDYADDLAAGNVAIAQAYSGDVVQLKADNPDLEFIVPQSGGTWF
HHHHHHCCCCEEECCCCCHHHHHHCCCEEEEEECCCCEEEEECCCCCEEEEEECCCCCEE
IDTMVIPYTTQNQAAAEAWIDYVYDRANYAKLIAFTQFVPVLSEMTDELNKIDPGVAANP
EEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LINPPESTVNNLKSWAALTDEQTQEYNTMYAAVTGG
CCCCCHHHHHHHHHHHHHCCHHHHHHCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]