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The map label for this gene is potB [H]

Identifier: 119868645

GI number: 119868645

Start: 2744397

End: 2745272

Strand: Reverse

Name: potB [H]

Synonym: Mkms_2610

Alternate gene names: 119868645

Gene position: 2745272-2744397 (Counterclockwise)

Preceding gene: 119868646

Following gene: 119868644

Centisome position: 47.85

GC content: 62.9

Gene sequence:

>876_bases
ATGGCAGGTGTAGCCAGCAGTAGCCGGCAGCGCAGCAGGATCGCTCCCTATCTGATGATCCTGCCCGCGCTGGCTTATCT
CGCAATCTTTTTCGTGTGGCCGCTGATCTCGCTGGCGCGCACGTCGCTGTCGTCGACCAGCGGGTCGGTGTTCCTGCCGA
CCCTCACCTTCTCGTGGGATTTCGCCAATTACAGTGAGGCGTTCAGCAGGTACTCCGATCAGATCATGCGGTCCTTCGGC
TATGCGCTGACCGCGACGATCGTGTGTCTGCTGCTGGCCTATCCGCTCGCCTACGTCATCGCGTTCAAGGCCGGCCGGTT
CAAGAACCTGATCCTCGGCCTGGTCATCCTGCCGTTCTTCGTGACGTTCCTGATCCGCACGATCGCATGGAAGACCATTC
TGGCCGACGACGGCTTGGTGGTGAGCGCGCTGGGCGCGATCGGGTTGCTGCCCAGCGACGGCCGCCTGCTGTCCACCAGC
TGGGCGGTCATCGGCGGGCTGATCTACAACTGGATCATCTTCATGATCCTGCCGCTGTACGTCAGCCTCGAGAAGATCGA
CCCGCGGCTCATCGAGGCGTCGAGGGATCTGTACTCGTCGAACACCCGCAGCTTTGCGAGGGTGATCCTGCCGCTGTCGC
TGCCCGGTGTGCTGGCGGGCAGCCTGCTGGTGTTCATCCCGGCGGCCGGCGACTTCATCAACGCCGAATACCTCGGCAGT
ACGCAGACCACGATGATAGGCAACGTGATCCAACAGCAGTTCCTCGTCGTCAAGGACTATCCGGCGGCCGCGGCGCTGAG
CCTGGTTTTGATGGGCACGATCCTGGCCGGTGTGCTGCTCTACACGCGGGCGCTGGGCACGGAGGACCTGGTATGA

Upstream 100 bases:

>100_bases
GACGGTGAACAACCTCAAGTCGTGGGCGGCGCTGACCGATGAGCAGACGCAGGAGTACAACACCATGTACGCCGCAGTGA
CCGGGGGCTGACGCCCACCC

Downstream 100 bases:

>100_bases
CCACCCAAGCCGGCGCCGCGGTCGCCGCCGCGGCCGAGCAGCCGCCCCGACCGAAAACCGTTCGGGGAACTCCGAAGTGG
GGCGACTGGAGCCTGCGGAT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAGVASSSRQRSRIAPYLMILPALAYLAIFFVWPLISLARTSLSSTSGSVFLPTLTFSWDFANYSEAFSRYSDQIMRSFG
YALTATIVCLLLAYPLAYVIAFKAGRFKNLILGLVILPFFVTFLIRTIAWKTILADDGLVVSALGAIGLLPSDGRLLSTS
WAVIGGLIYNWIIFMILPLYVSLEKIDPRLIEASRDLYSSNTRSFARVILPLSLPGVLAGSLLVFIPAAGDFINAEYLGS
TQTTMIGNVIQQQFLVVKDYPAAAALSLVLMGTILAGVLLYTRALGTEDLV

Sequences:

>Translated_291_residues
MAGVASSSRQRSRIAPYLMILPALAYLAIFFVWPLISLARTSLSSTSGSVFLPTLTFSWDFANYSEAFSRYSDQIMRSFG
YALTATIVCLLLAYPLAYVIAFKAGRFKNLILGLVILPFFVTFLIRTIAWKTILADDGLVVSALGAIGLLPSDGRLLSTS
WAVIGGLIYNWIIFMILPLYVSLEKIDPRLIEASRDLYSSNTRSFARVILPLSLPGVLAGSLLVFIPAAGDFINAEYLGS
TQTTMIGNVIQQQFLVVKDYPAAAALSLVLMGTILAGVLLYTRALGTEDLV
>Mature_290_residues
AGVASSSRQRSRIAPYLMILPALAYLAIFFVWPLISLARTSLSSTSGSVFLPTLTFSWDFANYSEAFSRYSDQIMRSFGY
ALTATIVCLLLAYPLAYVIAFKAGRFKNLILGLVILPFFVTFLIRTIAWKTILADDGLVVSALGAIGLLPSDGRLLSTSW
AVIGGLIYNWIIFMILPLYVSLEKIDPRLIEASRDLYSSNTRSFARVILPLSLPGVLAGSLLVFIPAAGDFINAEYLGST
QTTMIGNVIQQQFLVVKDYPAAAALSLVLMGTILAGVLLYTRALGTEDLV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=269, Percent_Identity=37.546468401487, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787080, Length=213, Percent_Identity=37.5586854460094, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1787713, Length=234, Percent_Identity=33.7606837606838, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31675; Mature: 31544

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVASSSRQRSRIAPYLMILPALAYLAIFFVWPLISLARTSLSSTSGSVFLPTLTFSWD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEC
FANYSEAFSRYSDQIMRSFGYALTATIVCLLLAYPLAYVIAFKAGRFKNLILGLVILPFF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VTFLIRTIAWKTILADDGLVVSALGAIGLLPSDGRLLSTSWAVIGGLIYNWIIFMILPLY
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
VSLEKIDPRLIEASRDLYSSNTRSFARVILPLSLPGVLAGSLLVFIPAAGDFINAEYLGS
HHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHEEEECCCCCCCHHHCCC
TQTTMIGNVIQQQFLVVKDYPAAAALSLVLMGTILAGVLLYTRALGTEDLV
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AGVASSSRQRSRIAPYLMILPALAYLAIFFVWPLISLARTSLSSTSGSVFLPTLTFSWD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEC
FANYSEAFSRYSDQIMRSFGYALTATIVCLLLAYPLAYVIAFKAGRFKNLILGLVILPFF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VTFLIRTIAWKTILADDGLVVSALGAIGLLPSDGRLLSTSWAVIGGLIYNWIIFMILPLY
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
VSLEKIDPRLIEASRDLYSSNTRSFARVILPLSLPGVLAGSLLVFIPAAGDFINAEYLGS
HHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHEEEECCCCCCCHHHCCC
TQTTMIGNVIQQQFLVVKDYPAAAALSLVLMGTILAGVLLYTRALGTEDLV
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]