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The map label for this gene is sigB [H]

Identifier: 119868259

GI number: 119868259

Start: 2338891

End: 2339850

Strand: Reverse

Name: sigB [H]

Synonym: Mkms_2223

Alternate gene names: 119868259

Gene position: 2339850-2338891 (Counterclockwise)

Preceding gene: 119868260

Following gene: 119868258

Centisome position: 40.78

GC content: 67.19

Gene sequence:

>960_bases
ATGGCAAATGCCACCACAAGCCGCGTCGATAGCGATCTGGACGCTCAGAGCCCGGCCGCTGACCTCGTGCGCGTGTATCT
CAACGGCATCGGCAAAACGGCCCTGCTGAACGCCGCCGACGAAGTCGAGCTGGCCAAGCGCATTGAGGCCGGGCTGTACG
CCCAGCATCTGCTGAACACGAAGAAGCGTCTCGGCGAGAGCCGCAAGCGCGATCTGGCCGCGGTCGTCCGCGACGGCGAG
GCCGCCCGCCGGCACCTGCTCGAGGCCAACCTGCGTCTCGTGGTGTCGCTGGCCAAGCGGTACACCGGCCGCGGCATGCC
GCTGCTCGACCTCATCCAGGAGGGCAACCTCGGCCTGATCCGCGCCATGGAGAAGTTCGACTACACCAAGGGCTTCAAGT
TCTCGACGTACGCGACGTGGTGGATCCGTCAGGCCATCACCCGCGGCATGGCCGATCAGAGCCGCACCATCCGGCTGCCT
GTGCACCTGGTCGAACAGGTCAACAAGCTGGCCCGGATCAAGCGCGAGATGCACCAGAACCTGGGCCGCGAGGCCACCGA
CGAGGAACTCGCCGAGGAGTCCGGGATCCCGGCCGAGAAGATCGCCGATCTGCTCGAGCACAGCCGCGATCCGGTGAGCC
TGGACATGCCGGTCGGCAGCGACGAAGAGGCCCCGCTGGGCGACTTCATCGAGGACGCCGAGGCGATGTCGGCGGAGAAC
GCGGTGATCTCGGAACTGCTGCACACCGACATCCGGCACGTGCTGGCCACGCTCGACGAGCGTGAGCAGCAGGTCATCCG
GTTGCGGTTCGGACTCGACGACGGGCAGCCGCGCACGCTGGACCAGATCGGCAAGCTCTTCGGGCTCTCCCGCGAACGGG
TCCGCCAGATCGAGCGCGAAGTGATGGCCAAGCTGCGCAACGGCGAACGCGCCGACCGACTGCGCTCCTACGCGAGCTGA

Upstream 100 bases:

>100_bases
AACCGCTGATCAAGACGTGTGAAGCAGGCGATACCCGGGAACTACTGACGGCACTTGAACGTTGAAGCTCATGACAGACC
GAGCTGATCAGGAGGCCGTT

Downstream 100 bases:

>100_bases
CCGAAACCCCAACAGGCATCGTGCCCGCCGGTCACACCGGCGGGCACGATGTCGTCAACCCGACATCCGGCCGTGTGTGT
CGTCGCCGATGGAGACCGCA

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MANATTSRVDSDLDAQSPAADLVRVYLNGIGKTALLNAADEVELAKRIEAGLYAQHLLNTKKRLGESRKRDLAAVVRDGE
AARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLP
VHLVEQVNKLARIKREMHQNLGREATDEELAEESGIPAEKIADLLEHSRDPVSLDMPVGSDEEAPLGDFIEDAEAMSAEN
AVISELLHTDIRHVLATLDEREQQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQIEREVMAKLRNGERADRLRSYAS

Sequences:

>Translated_319_residues
MANATTSRVDSDLDAQSPAADLVRVYLNGIGKTALLNAADEVELAKRIEAGLYAQHLLNTKKRLGESRKRDLAAVVRDGE
AARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLP
VHLVEQVNKLARIKREMHQNLGREATDEELAEESGIPAEKIADLLEHSRDPVSLDMPVGSDEEAPLGDFIEDAEAMSAEN
AVISELLHTDIRHVLATLDEREQQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQIEREVMAKLRNGERADRLRSYAS
>Mature_318_residues
ANATTSRVDSDLDAQSPAADLVRVYLNGIGKTALLNAADEVELAKRIEAGLYAQHLLNTKKRLGESRKRDLAAVVRDGEA
ARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLPV
HLVEQVNKLARIKREMHQNLGREATDEELAEESGIPAEKIADLLEHSRDPVSLDMPVGSDEEAPLGDFIEDAEAMSAENA
VISELLHTDIRHVLATLDEREQQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQIEREVMAKLRNGERADRLRSYAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=271, Percent_Identity=48.3394833948339, Blast_Score=270, Evalue=7e-74,
Organism=Escherichia coli, GI1789098, Length=286, Percent_Identity=40.2097902097902, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1789871, Length=246, Percent_Identity=31.3008130081301, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788231, Length=203, Percent_Identity=29.064039408867, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35886; Mature: 35755

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANATTSRVDSDLDAQSPAADLVRVYLNGIGKTALLNAADEVELAKRIEAGLYAQHLLNT
CCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
KKRLGESRKRDLAAVVRDGEAARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHH
RAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLPVHLVEQVNKLARIKREMHQN
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LGREATDEELAEESGIPAEKIADLLEHSRDPVSLDMPVGSDEEAPLGDFIEDAEAMSAEN
HCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
AVISELLHTDIRHVLATLDEREQQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQIERE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
VMAKLRNGERADRLRSYAS
HHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
ANATTSRVDSDLDAQSPAADLVRVYLNGIGKTALLNAADEVELAKRIEAGLYAQHLLNT
CCCCHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
KKRLGESRKRDLAAVVRDGEAARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHH
RAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLPVHLVEQVNKLARIKREMHQN
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LGREATDEELAEESGIPAEKIADLLEHSRDPVSLDMPVGSDEEAPLGDFIEDAEAMSAEN
HCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
AVISELLHTDIRHVLATLDEREQQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQIERE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
VMAKLRNGERADRLRSYAS
HHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910; 2116013 [H]