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The map label for this gene is gluB [H]

Identifier: 119868237

GI number: 119868237

Start: 2314651

End: 2315478

Strand: Reverse

Name: gluB [H]

Synonym: Mkms_2201

Alternate gene names: 119868237

Gene position: 2315478-2314651 (Counterclockwise)

Preceding gene: 119868238

Following gene: 119868236

Centisome position: 40.36

GC content: 66.3

Gene sequence:

>828_bases
ATGCCGTCCTTCTCGATGCTGTCTCGCAGGCGCGTCACGGCCGCCGTCGCGCTGGCGCTCGCTGTGCCGTTCGCGGCCAC
CGCGTGCGGAGGTGGCGGCGGTGACGAGAACACCATCGTCATCGGCACGAAGTTCGACCAGCCGGGCCTGGGCCTGCGCA
ACCCCGACGGTTCGATGAGCGGCTTCGACGTCGACGTCGCGACCTACGTGGCCGGCGAACTGGGATACACCCCCGAGCAG
ATCGAGTGGAAGGAATCGCCGTCCGGACAGCGTGAGACGTTGATCCAGAACGACCAGGTCGACTACATCGTGGCGACCTA
TTCGATCACCGACTCCCGCAAGGAGAAGGTCGACTTCGCCGGGCCGTACCTGATCACCGGCCAGAGCCTGCTGGTGCGCA
ACGACAACACCGACATCACCGGCGCCGCGTCGCTGGCCAACAACAAGCGGCTGTGCTCGGTGTCGGGTTCGACTCCCGCG
CAGAAGATCAAGGACGAGTACCCGGGTGTGCAGCTGCAGCAGTACGACACCTACTCGGCGTGCATCGAGGCGCTGAAGAA
CGGCGCCATCGACGCGGTCACCACCGACGAGGTGATCCTGGCCGGTTACGCCGCGCAGACCCCGGGCGCCTTCAAGATCG
TCGGGGACACGTTCTCCGAGGAGCGCTACGGCGTCGGGCTGAAGAAGGACGACAGCGAACTGCGGGCCAAGATCAACGCC
GCGATCGAGAAGATGGAGTCCAGCGGCGCCTGGAAGGAAGCCTTCGACAAGAACCTCGGCCCGGCGGGTATCACGGCGCC
CACCCCGCCGCCGGTCGACAGCAACTGA

Upstream 100 bases:

>100_bases
AGCCGGCCCAGTTCTTCGACAATCCGCAGACCGACCGCGCCAAGGACTTCCTCGGCAAGATCCTCCATCACTAAACGCCC
GGCGAAAGGAAACCCCACCC

Downstream 100 bases:

>100_bases
GTCGAGGGTCTGGCTCCGGCCGTGGAGATATTCACCGAGTACCGCGCGCAGATCTTCGAGGCGTTCTGGACCACCATCCA
GCTCACGGTCTACTCCGCGG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPSFSMLSRRRVTAAVALALAVPFAATACGGGGGDENTIVIGTKFDQPGLGLRNPDGSMSGFDVDVATYVAGELGYTPEQ
IEWKESPSGQRETLIQNDQVDYIVATYSITDSRKEKVDFAGPYLITGQSLLVRNDNTDITGAASLANNKRLCSVSGSTPA
QKIKDEYPGVQLQQYDTYSACIEALKNGAIDAVTTDEVILAGYAAQTPGAFKIVGDTFSEERYGVGLKKDDSELRAKINA
AIEKMESSGAWKEAFDKNLGPAGITAPTPPPVDSN

Sequences:

>Translated_275_residues
MPSFSMLSRRRVTAAVALALAVPFAATACGGGGGDENTIVIGTKFDQPGLGLRNPDGSMSGFDVDVATYVAGELGYTPEQ
IEWKESPSGQRETLIQNDQVDYIVATYSITDSRKEKVDFAGPYLITGQSLLVRNDNTDITGAASLANNKRLCSVSGSTPA
QKIKDEYPGVQLQQYDTYSACIEALKNGAIDAVTTDEVILAGYAAQTPGAFKIVGDTFSEERYGVGLKKDDSELRAKINA
AIEKMESSGAWKEAFDKNLGPAGITAPTPPPVDSN
>Mature_274_residues
PSFSMLSRRRVTAAVALALAVPFAATACGGGGGDENTIVIGTKFDQPGLGLRNPDGSMSGFDVDVATYVAGELGYTPEQI
EWKESPSGQRETLIQNDQVDYIVATYSITDSRKEKVDFAGPYLITGQSLLVRNDNTDITGAASLANNKRLCSVSGSTPAQ
KIKDEYPGVQLQQYDTYSACIEALKNGAIDAVTTDEVILAGYAAQTPGAFKIVGDTFSEERYGVGLKKDDSELRAKINAA
IEKMESSGAWKEAFDKNLGPAGITAPTPPPVDSN

Specific function: Part of the binding-protein-dependent transport system for glutamate [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=264, Percent_Identity=24.6212121212121, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787031, Length=203, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788648, Length=218, Percent_Identity=27.5229357798165, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787088, Length=229, Percent_Identity=26.2008733624454, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788649, Length=257, Percent_Identity=25.6809338521401, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=21.9123505976096, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29147; Mature: 29016

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSFSMLSRRRVTAAVALALAVPFAATACGGGGGDENTIVIGTKFDQPGLGLRNPDGSMS
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
GFDVDVATYVAGELGYTPEQIEWKESPSGQRETLIQNDQVDYIVATYSITDSRKEKVDFA
CCCCHHHHHHHHHCCCCCCCEEECCCCCCCHHHHCCCCCCCEEEEEEEECCCCHHCCCCC
GPYLITGQSLLVRNDNTDITGAASLANNKRLCSVSGSTPAQKIKDEYPGVQLQQYDTYSA
CCEEEECCEEEEECCCCCCEEHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEECCCHHH
CIEALKNGAIDAVTTDEVILAGYAAQTPGAFKIVGDTFSEERYGVGLKKDDSELRAKINA
HHHHHHCCCEEEEECCCEEEEEECCCCCCEEEEECCCCCCHHHCCCCCCCHHHHHHHHHH
AIEKMESSGAWKEAFDKNLGPAGITAPTPPPVDSN
HHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PSFSMLSRRRVTAAVALALAVPFAATACGGGGGDENTIVIGTKFDQPGLGLRNPDGSMS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
GFDVDVATYVAGELGYTPEQIEWKESPSGQRETLIQNDQVDYIVATYSITDSRKEKVDFA
CCCCHHHHHHHHHCCCCCCCEEECCCCCCCHHHHCCCCCCCEEEEEEEECCCCHHCCCCC
GPYLITGQSLLVRNDNTDITGAASLANNKRLCSVSGSTPAQKIKDEYPGVQLQQYDTYSA
CCEEEECCEEEEECCCCCCEEHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEECCCHHH
CIEALKNGAIDAVTTDEVILAGYAAQTPGAFKIVGDTFSEERYGVGLKKDDSELRAKINA
HHHHHHCCCEEEEECCCEEEEEECCCCCCEEEEECCCCCCHHHCCCCCCCHHHHHHHHHH
AIEKMESSGAWKEAFDKNLGPAGITAPTPPPVDSN
HHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7868586; 12948626 [H]