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The map label for this gene is asnB [H]

Identifier: 119868137

GI number: 119868137

Start: 2223433

End: 2225238

Strand: Reverse

Name: asnB [H]

Synonym: Mkms_2101

Alternate gene names: 119868137

Gene position: 2225238-2223433 (Counterclockwise)

Preceding gene: 119868138

Following gene: 119868136

Centisome position: 38.79

GC content: 70.16

Gene sequence:

>1806_bases
ATGTGTGGAGCCACCGGCGAGGTACGCCTCGACGGCCGAACCCCTGACCTAGCCGCCGTCTCGGCGATGGCCGAGACGTT
GTCACCGCGCGGCCCGGACGGCGCAGGCGCCTGGTCGCAGGGCCGGGTCGCGCTGGGACACCGCCGCCTCAAGATCATCG
ACCTGTCCGAGGCGGGCGCGCAGCCCATGGTCGACTCCGACCTCGGCCTGGCCATCGCGTGGAACGGCTGCATCTACAAC
TATCAGCAGCTGCGTGACGAACTCCGCGGCCACGGCTACAGCTTCTTCTCCACCAGCGACACCGAGGTGCTGCTCAAGGC
CTACCACCACTGGGGCGACCGGTTCGTCGACCGCCTCTACGGGATGTTCGCGTTCGCGATCGTCGAACGCGACAGCGGAC
GGGTTCTGCTCGGCCGCGACCGGCTGGGCATCAAACCGCTCTACATCTCCGAGGACGCCCACCGGATCCGCTTCGCCTCG
TCGTTGCCCGCCCTGGTCGCCGGCGGCGGCGTGGACACCCGCATCGATCCGGTCGCGCTGCACCACTACCTGTCCTTCCA
CTCCGTCGTGCCGCCACCGCTGACGATCCTGCGCGGTGTGAAGAAGGTGCCGCCGGCGTCGCTGGTCGCCATCGAACCCG
ACGGCAGGCGCACCACCACGACGTACTGGACCCCGGACTTCACCCGGCACGCCGATCGCGCCGACTGGTCCGAACAGGAC
TGGGAGGACGCGATGCTCACCGCGCTGCGGCGCGCCGTCGAGCGCCGGCTGGTCTCCGACGTGCCGGTGGGGTGTCTGCT
GTCGGGCGGCGTCGACTCGAGCTTGATCGTGGGCCTGCTCGCCGAGGCCGGTCAGACCGGGCTGCAGACCTTCTCGATCG
GCTTCGAATCGGTCAACGGCGTGGCCGGTGACGAGTTCAAGTACTCCGACATCGTCGCGCAGCGGTTCGGCACCGACCAC
CACCAGATCCGCATCGACACGGCGCGGATGCTGCCCGCCCTCGACGGTGCGATCGGCGCGATGAGCGAGCCGATGGTCAG
CCACGACTGCGTGGCCTTCTACCTGCTCAGCCAGGAGGTGGCCAAGTACGTCAAGGTGGTGCAGTCGGGTCAGGGCGCCG
ACGAGGTGTTCGCCGGCTACCACTGGTATCCGCCGATGGCCGAACCCGGCGCCGCATCGGTCGAGGGTTCGGTGGCCAGC
TACCGGGCGGCGTTCTTCGACCGCGACCCGGCGGCCCTCGGCGGGCTCGTCACGCCCCCGTACTTCGCCGACGGCGATCC
CAGCGGCCGGTTCGTCACCGAGCACTTCGCCCGCGAGGGCGCCGCCACCGGGGTGGACCGGGCGCTGCGCCTCGACACCA
TGGTCATGCTCGTCGACGACCCGGTGAAACGCGTGGACAACATGACGATGGCCTGGGGTCTGGAGGGCCGCGTGCCGTTC
CTCGACCACGAACTGGTTGAACTGGCCGCCACCTGTCCCCCCGAGTACAAGACCGCCCAGGGCGGTAAGGGCGTGCTCAA
GGAGGCCGCGCGCCGGGTGATCCCGGCCGAGGTCATCGACCGGCCCAAGGGGTACTTCCCGGTTCCCGCGCTCACCCACC
TCGAGGGTCCGTACCTCGACATGGTCCGCGACGCCCTGTCGGCGCCGGCCGCCAAGGAACGCGACCTGTTCCGGCCCGAG
GCCGTCGACCGGCTGCTGGCCGATCCGAACGGCCGGCTCACCCCGCTGCGCGGCAACGAACTGTGGCAGATGGCGCTGCT
GGAGCTGTGGCTGCAGCGACACGGGATCACCGGACCGGCCGCATGA

Upstream 100 bases:

>100_bases
GCAACGGGGGTACCACCCGCGCGTGAGAACGAACGATCCGCGGATGGCGATTCACTCGGCAGCACCGCGCCCCGAACGCG
GCCACGGTAGGGTCTAAGCG

Downstream 100 bases:

>100_bases
CCGACCTCGGATCGCACCTCGATCCCGACAGCGACCACACCGAGGCCATCACCCTCGGCCTGCACGACGCGTCGCCACAG
CATCTGGTGGACCGGATGGC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MCGATGEVRLDGRTPDLAAVSAMAETLSPRGPDGAGAWSQGRVALGHRRLKIIDLSEAGAQPMVDSDLGLAIAWNGCIYN
YQQLRDELRGHGYSFFSTSDTEVLLKAYHHWGDRFVDRLYGMFAFAIVERDSGRVLLGRDRLGIKPLYISEDAHRIRFAS
SLPALVAGGGVDTRIDPVALHHYLSFHSVVPPPLTILRGVKKVPPASLVAIEPDGRRTTTTYWTPDFTRHADRADWSEQD
WEDAMLTALRRAVERRLVSDVPVGCLLSGGVDSSLIVGLLAEAGQTGLQTFSIGFESVNGVAGDEFKYSDIVAQRFGTDH
HQIRIDTARMLPALDGAIGAMSEPMVSHDCVAFYLLSQEVAKYVKVVQSGQGADEVFAGYHWYPPMAEPGAASVEGSVAS
YRAAFFDRDPAALGGLVTPPYFADGDPSGRFVTEHFAREGAATGVDRALRLDTMVMLVDDPVKRVDNMTMAWGLEGRVPF
LDHELVELAATCPPEYKTAQGGKGVLKEAARRVIPAEVIDRPKGYFPVPALTHLEGPYLDMVRDALSAPAAKERDLFRPE
AVDRLLADPNGRLTPLRGNELWQMALLELWLQRHGITGPAA

Sequences:

>Translated_601_residues
MCGATGEVRLDGRTPDLAAVSAMAETLSPRGPDGAGAWSQGRVALGHRRLKIIDLSEAGAQPMVDSDLGLAIAWNGCIYN
YQQLRDELRGHGYSFFSTSDTEVLLKAYHHWGDRFVDRLYGMFAFAIVERDSGRVLLGRDRLGIKPLYISEDAHRIRFAS
SLPALVAGGGVDTRIDPVALHHYLSFHSVVPPPLTILRGVKKVPPASLVAIEPDGRRTTTTYWTPDFTRHADRADWSEQD
WEDAMLTALRRAVERRLVSDVPVGCLLSGGVDSSLIVGLLAEAGQTGLQTFSIGFESVNGVAGDEFKYSDIVAQRFGTDH
HQIRIDTARMLPALDGAIGAMSEPMVSHDCVAFYLLSQEVAKYVKVVQSGQGADEVFAGYHWYPPMAEPGAASVEGSVAS
YRAAFFDRDPAALGGLVTPPYFADGDPSGRFVTEHFAREGAATGVDRALRLDTMVMLVDDPVKRVDNMTMAWGLEGRVPF
LDHELVELAATCPPEYKTAQGGKGVLKEAARRVIPAEVIDRPKGYFPVPALTHLEGPYLDMVRDALSAPAAKERDLFRPE
AVDRLLADPNGRLTPLRGNELWQMALLELWLQRHGITGPAA
>Mature_601_residues
MCGATGEVRLDGRTPDLAAVSAMAETLSPRGPDGAGAWSQGRVALGHRRLKIIDLSEAGAQPMVDSDLGLAIAWNGCIYN
YQQLRDELRGHGYSFFSTSDTEVLLKAYHHWGDRFVDRLYGMFAFAIVERDSGRVLLGRDRLGIKPLYISEDAHRIRFAS
SLPALVAGGGVDTRIDPVALHHYLSFHSVVPPPLTILRGVKKVPPASLVAIEPDGRRTTTTYWTPDFTRHADRADWSEQD
WEDAMLTALRRAVERRLVSDVPVGCLLSGGVDSSLIVGLLAEAGQTGLQTFSIGFESVNGVAGDEFKYSDIVAQRFGTDH
HQIRIDTARMLPALDGAIGAMSEPMVSHDCVAFYLLSQEVAKYVKVVQSGQGADEVFAGYHWYPPMAEPGAASVEGSVAS
YRAAFFDRDPAALGGLVTPPYFADGDPSGRFVTEHFAREGAATGVDRALRLDTMVMLVDDPVKRVDNMTMAWGLEGRVPF
LDHELVELAATCPPEYKTAQGGKGVLKEAARRVIPAEVIDRPKGYFPVPALTHLEGPYLDMVRDALSAPAAKERDLFRPE
AVDRLLADPNGRLTPLRGNELWQMALLELWLQRHGITGPAA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=603, Percent_Identity=24.0464344941957, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI168229252, Length=603, Percent_Identity=24.0464344941957, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI168229250, Length=603, Percent_Identity=24.0464344941957, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI296010848, Length=577, Percent_Identity=23.9168110918544, Blast_Score=123, Evalue=6e-28,
Organism=Homo sapiens, GI296010852, Length=507, Percent_Identity=24.0631163708087, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI296010850, Length=507, Percent_Identity=24.0631163708087, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1786889, Length=410, Percent_Identity=30.9756097560976, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25147557, Length=556, Percent_Identity=27.5179856115108, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71993933, Length=556, Percent_Identity=27.5179856115108, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6325403, Length=577, Percent_Identity=27.0363951473137, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321563, Length=576, Percent_Identity=25.6944444444444, Blast_Score=139, Evalue=9e-34,
Organism=Drosophila melanogaster, GI45553209, Length=383, Percent_Identity=30.5483028720627, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 65526; Mature: 65526

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGATGEVRLDGRTPDLAAVSAMAETLSPRGPDGAGAWSQGRVALGHRRLKIIDLSEAGA
CCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCC
QPMVDSDLGLAIAWNGCIYNYQQLRDELRGHGYSFFSTSDTEVLLKAYHHWGDRFVDRLY
CCCCCCCCCEEEEECCHHCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
GMFAFAIVERDSGRVLLGRDRLGIKPLYISEDAHRIRFASSLPALVAGGGVDTRIDPVAL
HHHHHHEEECCCCCEEEECCCCCCEEEEECCCCHHEEEHHCCCHHHCCCCCCCCCCHHHH
HHYLSFHSVVPPPLTILRGVKKVPPASLVAIEPDGRRTTTTYWTPDFTRHADRADWSEQD
HHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCHHHCCCCCCCCCCC
WEDAMLTALRRAVERRLVSDVPVGCLLSGGVDSSLIVGLLAEAGQTGLQTFSIGFESVNG
HHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHCCCCCCEEEEECHHHCCC
VAGDEFKYSDIVAQRFGTDHHQIRIDTARMLPALDGAIGAMSEPMVSHDCVAFYLLSQEV
CCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AKYVKVVQSGQGADEVFAGYHWYPPMAEPGAASVEGSVASYRAAFFDRDPAALGGLVTPP
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCCC
YFADGDPSGRFVTEHFAREGAATGVDRALRLDTMVMLVDDPVKRVDNMTMAWGLEGRVPF
CCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHEEEEEECCHHHHHCCCEEEECCCCCCCC
LDHELVELAATCPPEYKTAQGGKGVLKEAARRVIPAEVIDRPKGYFPVPALTHLEGPYLD
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHCCCHHHH
MVRDALSAPAAKERDLFRPEAVDRLLADPNGRLTPLRGNELWQMALLELWLQRHGITGPA
HHHHHHCCCCCHHHCCCCHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCC
A
C
>Mature Secondary Structure
MCGATGEVRLDGRTPDLAAVSAMAETLSPRGPDGAGAWSQGRVALGHRRLKIIDLSEAGA
CCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCC
QPMVDSDLGLAIAWNGCIYNYQQLRDELRGHGYSFFSTSDTEVLLKAYHHWGDRFVDRLY
CCCCCCCCCEEEEECCHHCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
GMFAFAIVERDSGRVLLGRDRLGIKPLYISEDAHRIRFASSLPALVAGGGVDTRIDPVAL
HHHHHHEEECCCCCEEEECCCCCCEEEEECCCCHHEEEHHCCCHHHCCCCCCCCCCHHHH
HHYLSFHSVVPPPLTILRGVKKVPPASLVAIEPDGRRTTTTYWTPDFTRHADRADWSEQD
HHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCHHHCCCCCCCCCCC
WEDAMLTALRRAVERRLVSDVPVGCLLSGGVDSSLIVGLLAEAGQTGLQTFSIGFESVNG
HHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHCCCCCCEEEEECHHHCCC
VAGDEFKYSDIVAQRFGTDHHQIRIDTARMLPALDGAIGAMSEPMVSHDCVAFYLLSQEV
CCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AKYVKVVQSGQGADEVFAGYHWYPPMAEPGAASVEGSVASYRAAFFDRDPAALGGLVTPP
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCCC
YFADGDPSGRFVTEHFAREGAATGVDRALRLDTMVMLVDDPVKRVDNMTMAWGLEGRVPF
CCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHEEEEEECCHHHHHCCCEEEECCCCCCCC
LDHELVELAATCPPEYKTAQGGKGVLKEAARRVIPAEVIDRPKGYFPVPALTHLEGPYLD
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHCCCHHHH
MVRDALSAPAAKERDLFRPEAVDRLLADPNGRLTPLRGNELWQMALLELWLQRHGITGPA
HHHHHHCCCCCHHHCCCCHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]