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The map label for this gene is phrA [H]

Identifier: 119868120

GI number: 119868120

Start: 2205719

End: 2207056

Strand: Reverse

Name: phrA [H]

Synonym: Mkms_2084

Alternate gene names: 119868120

Gene position: 2207056-2205719 (Counterclockwise)

Preceding gene: 119868122

Following gene: 119868119

Centisome position: 38.47

GC content: 70.55

Gene sequence:

>1338_bases
ATGCCCACGCTGTTGTGGTTCCGCCGCGATCTGCGCCTGCACGATCTGCCCGCGCTGGTCGATGCGGCCCAGGGCGACGG
TCAGGTGCTCGCCTGCTATGTGCTGGATCCGAGACTGCACAGGTCGGCGGGCCCGCGGCGGCTGCAGTACCTGCACGACG
CCCTGCGGGATCTGCGCGACCAGCTCGACGGCCGGCTGCTGGTGACCCGCGGCCGCCCGGAGCAGCGGATCCCGGCGCTG
GCGAAGAGCATCGACGCGTCGGCGGTGTACGTCTCCGGCGACTTCACCCCGTTCGGACGGCGACGCGATGACGCCGTCCG
GAAAGCTCTGGGCGAGGTTCCCCTCCAACCGTCCGGTTCGCCCTATCTGGTGTCGCCGGGCCGGGTCACCAAGGGCGACG
GGACGCCGTACAAGGTGTTCAGCCCGTTCTTCGACGCCTGGCGCAGACACGGTTGGCGCGCGCCGGCACAGAGCGGGCCG
GATTCGGCGACGTGGATCGACCCGGCGGATCTGACGGGCCGCGATCTGCGGACCGAGATCCCCGACGACGGCGCCACCCT
GGACATCCCCGCGGGTGAGCGCGCCGCGGCGCAGCACTGGCGGGCGTTCGTCGCCGACGAACTCGACGGTTACGCCGACA
ACCGCAACCGCCCGGACCTCGACGTCACCAGCCGGATGTCGGCGCACCTGAAGTTCGGGACCATCCATCCCCGCACGATG
GTCGACGACCTCGGGCGGGGCAAAGGCGCCCAGGCGTATCTGCGGGAACTGGCGTTCCGCGACTTCTACGCGGCGGTGCT
CCACGAGTGGCCCCGCAGCGTGTGGTGGAACTGGAACACCGGGTTCGACGGCATCCGCGTCGACGAGGGTGCGGTGGCCG
AGCAGCGCTTCGACGCGTGGAAGCGCGGGCGCACCGGGTTCCCGATCGTCGACGCCGGGATGCGTCAACTCGCCGGGATC
GGCTGGATGCACAACCGGGTCCGGATGATCGTGGCCTCGTTCCTGGTCAAGGACCTGCACCTGCCGTGGCAGTGGGGGGC
GCGCTGGTTCCTCGAGCAGCTGGTCGACGGCGATATGGCCAACAACCAGCACGGGTGGCAGTGGACCGCGGGGTGCGGCA
CCGACGCCGCACCGTTCTTCCGGGTGTTCAACCCCTCGACGCAGGGCGCGAAGTTCGATCCGGACGGCACGTACGTGCGG
CGGTGGGTGCCCGAACTGAAGGGGGTGGCCGACGTGCACAAGATGGGTGACGATCGCCCGGCGGACTACCCCGCACCCAT
CGTCGACCATGCGGCCGAACGGGCCGAGGCGCTGCGCCGCTACGCCGAGATCTCCTAG

Upstream 100 bases:

>100_bases
ATTGTTGCAGGCGCTGCGCGGGCGCGCCCGGTGCGGACCGGTCATGGTTCCGTCATCACGGCGGGGTTTCCCACCCCGGG
CATCGGGGCAGAAGCAAGTC

Downstream 100 bases:

>100_bases
GGCACTCCGGTTACGACGCAGCGTCGTATGCCAGAATGACCGCATCGCGTTTCGACTGTGAGGAGCACTCTGTGGCCAAC
ACCGAGGTGGAACGACACAC

Product: deoxyribodipyrimidine photo-lyase type I

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MPTLLWFRRDLRLHDLPALVDAAQGDGQVLACYVLDPRLHRSAGPRRLQYLHDALRDLRDQLDGRLLVTRGRPEQRIPAL
AKSIDASAVYVSGDFTPFGRRRDDAVRKALGEVPLQPSGSPYLVSPGRVTKGDGTPYKVFSPFFDAWRRHGWRAPAQSGP
DSATWIDPADLTGRDLRTEIPDDGATLDIPAGERAAAQHWRAFVADELDGYADNRNRPDLDVTSRMSAHLKFGTIHPRTM
VDDLGRGKGAQAYLRELAFRDFYAAVLHEWPRSVWWNWNTGFDGIRVDEGAVAEQRFDAWKRGRTGFPIVDAGMRQLAGI
GWMHNRVRMIVASFLVKDLHLPWQWGARWFLEQLVDGDMANNQHGWQWTAGCGTDAAPFFRVFNPSTQGAKFDPDGTYVR
RWVPELKGVADVHKMGDDRPADYPAPIVDHAAERAEALRRYAEIS

Sequences:

>Translated_445_residues
MPTLLWFRRDLRLHDLPALVDAAQGDGQVLACYVLDPRLHRSAGPRRLQYLHDALRDLRDQLDGRLLVTRGRPEQRIPAL
AKSIDASAVYVSGDFTPFGRRRDDAVRKALGEVPLQPSGSPYLVSPGRVTKGDGTPYKVFSPFFDAWRRHGWRAPAQSGP
DSATWIDPADLTGRDLRTEIPDDGATLDIPAGERAAAQHWRAFVADELDGYADNRNRPDLDVTSRMSAHLKFGTIHPRTM
VDDLGRGKGAQAYLRELAFRDFYAAVLHEWPRSVWWNWNTGFDGIRVDEGAVAEQRFDAWKRGRTGFPIVDAGMRQLAGI
GWMHNRVRMIVASFLVKDLHLPWQWGARWFLEQLVDGDMANNQHGWQWTAGCGTDAAPFFRVFNPSTQGAKFDPDGTYVR
RWVPELKGVADVHKMGDDRPADYPAPIVDHAAERAEALRRYAEIS
>Mature_444_residues
PTLLWFRRDLRLHDLPALVDAAQGDGQVLACYVLDPRLHRSAGPRRLQYLHDALRDLRDQLDGRLLVTRGRPEQRIPALA
KSIDASAVYVSGDFTPFGRRRDDAVRKALGEVPLQPSGSPYLVSPGRVTKGDGTPYKVFSPFFDAWRRHGWRAPAQSGPD
SATWIDPADLTGRDLRTEIPDDGATLDIPAGERAAAQHWRAFVADELDGYADNRNRPDLDVTSRMSAHLKFGTIHPRTMV
DDLGRGKGAQAYLRELAFRDFYAAVLHEWPRSVWWNWNTGFDGIRVDEGAVAEQRFDAWKRGRTGFPIVDAGMRQLAGIG
WMHNRVRMIVASFLVKDLHLPWQWGARWFLEQLVDGDMANNQHGWQWTAGCGTDAAPFFRVFNPSTQGAKFDPDGTYVRR
WVPELKGVADVHKMGDDRPADYPAPIVDHAAERAEALRRYAEIS

Specific function: Involved in repair of UV radiation-induced DNA damage. Exhibits specific, light-dependent repair activity for a cyclobutyl pyrimidine dimer by catalyzing the light-dependent monomerization (300-600 nm) of the dimers (in cis-syn configuration), which are f

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=488, Percent_Identity=28.2786885245902, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI188536100, Length=492, Percent_Identity=28.4552845528455, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI188536103, Length=445, Percent_Identity=27.1910112359551, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786926, Length=488, Percent_Identity=34.6311475409836, Blast_Score=250, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6324962, Length=505, Percent_Identity=28.5148514851485, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI17137248, Length=506, Percent_Identity=28.8537549407115, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24585455, Length=506, Percent_Identity=28.8537549407115, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24648152, Length=501, Percent_Identity=27.1457085828343, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 50180; Mature: 50049

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLLWFRRDLRLHDLPALVDAAQGDGQVLACYVLDPRLHRSAGPRRLQYLHDALRDLRD
CCCHHHHHHCCCHHCCHHHHHCCCCCCCEEEEEEECCHHHHCCCHHHHHHHHHHHHHHHH
QLDGRLLVTRGRPEQRIPALAKSIDASAVYVSGDFTPFGRRRDDAVRKALGEVPLQPSGS
HCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCC
PYLVSPGRVTKGDGTPYKVFSPFFDAWRRHGWRAPAQSGPDSATWIDPADLTGRDLRTEI
CEEECCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PDDGATLDIPAGERAAAQHWRAFVADELDGYADNRNRPDLDVTSRMSAHLKFGTIHPRTM
CCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEECCCCCHHH
VDDLGRGKGAQAYLRELAFRDFYAAVLHEWPRSVWWNWNTGFDGIRVDEGAVAEQRFDAW
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCHHHHHHHHHH
KRGRTGFPIVDAGMRQLAGIGWMHNRVRMIVASFLVKDLHLPWQWGARWFLEQLVDGDMA
HCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
NNQHGWQWTAGCGTDAAPFFRVFNPSTQGAKFDPDGTYVRRWVPELKGVADVHKMGDDRP
CCCCCCEEECCCCCCCCCHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
ADYPAPIVDHAAERAEALRRYAEIS
CCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTLLWFRRDLRLHDLPALVDAAQGDGQVLACYVLDPRLHRSAGPRRLQYLHDALRDLRD
CCHHHHHHCCCHHCCHHHHHCCCCCCCEEEEEEECCHHHHCCCHHHHHHHHHHHHHHHH
QLDGRLLVTRGRPEQRIPALAKSIDASAVYVSGDFTPFGRRRDDAVRKALGEVPLQPSGS
HCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCC
PYLVSPGRVTKGDGTPYKVFSPFFDAWRRHGWRAPAQSGPDSATWIDPADLTGRDLRTEI
CEEECCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PDDGATLDIPAGERAAAQHWRAFVADELDGYADNRNRPDLDVTSRMSAHLKFGTIHPRTM
CCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEECCCCCHHH
VDDLGRGKGAQAYLRELAFRDFYAAVLHEWPRSVWWNWNTGFDGIRVDEGAVAEQRFDAW
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCHHHHHHHHHH
KRGRTGFPIVDAGMRQLAGIGWMHNRVRMIVASFLVKDLHLPWQWGARWFLEQLVDGDMA
HCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
NNQHGWQWTAGCGTDAAPFFRVFNPSTQGAKFDPDGTYVRRWVPELKGVADVHKMGDDRP
CCCCCCEEECCCCCCCCCHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
ADYPAPIVDHAAERAEALRRYAEIS
CCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11254140; 8905231; 10896222; 10871367 [H]