Click here to switch to the map view.

The map label for this gene is lcfB [H]

Identifier: 119868062

GI number: 119868062

Start: 2145389

End: 2147881

Strand: Reverse

Name: lcfB [H]

Synonym: Mkms_2025

Alternate gene names: 119868062

Gene position: 2147881-2145389 (Counterclockwise)

Preceding gene: 119868063

Following gene: 119868057

Centisome position: 37.44

GC content: 71.24

Gene sequence:

>2493_bases
ATGAGCCGCCTCGTCGATTGTCTTCGCGCGCACGGGGATCGGATCGCGGTACTCACCGATTCCGAGCGGCTCTCATACCG
CGACCTCGCCGACCTGGTGTCCGGGTCGGCCGCCCGTCTCGGGGACACCCGTCGTCTGGTGATGCTCGAGACCCGCAACG
ACCTGCCGACCCTGGTGCACTACCTGGGGGCGCTCGCCGGCGGCCACGTGGTGCTGCCGGTGCCTGCCGGACGCGATCAC
CGCGAGTTGACGGCCACCTACGATCCCGACGTCGTGGTCGACGGGTGCGGACCGCAGATCAGGCGCGAGGGCAGCGCACA
CCGCCTGCACGACGACCTGGCGCTGCTGTTGTCCACCTCGGGCAGCACCGGCTCACCCAAGCTGGTCCGGCTCTCGCGGC
GCAATCTGCTCAGCAATGCCCATGCGATCGCCGACTACCTCGACATCCGCGAAACCGACCGCGCCGCAACGACACTGCCG
ATGTCGTACTGCTACGGCCTGTCGGTGGTCCACAGCCACCTGCTGCGCGGCGCCGCGCTCGTCCTCACCGAACACTCCGT
GGTCGACGACGAGTTCTGGACGTTGTTCCGCCGCCACGGCGGCACCACCTTCGCCGGTGTGCCCTACACGTTCGACCTGC
TCGACCGCGCCGGTTTCGACACCATGGACCTGCCGCACCTGCGCTATGTCACCCAGGCCGGTGGACGGTTGGCGCCCGAA
CGGGTGAAACGGTACGCCGAACTCGGCGCGGCACGCGGCTGGCAGCTGATCGTCATGTACGGCGCGACCGAGGCGACGGC
CCGGATGGCCTACCTTCCGCCCGACCTCGCCGCCGAGCATCCCGGCTGCATCGGCGTACCGATCCCGGGCGGCTCGTTCA
CGATCGAACCGCTGGACGGCTGGCCGGCCGACGATGCCGCGGGCGAACTCGTCTACCGCGGGGACAACGTGATGATGGGC
TACGCGCACACCGCGGCCGATCTGTCGCGCGGCGACGAGGTCGAGGCGCTGCACACCGGCGACATCGCCCGCCGCCGGCC
CGACGGTCTCTACGAGGTGATCGGCCGCAGCAGCCGGTTCGTCAAGATGTACGGCCTGCGCATCGATCTGCAGCGGCTCG
AGACGGCGCTGCGTGCGCGTGGCGTGCCCGCGTTGTGCACCGACCTCGACGACCGCCTCGTCGTCGCCGCGACCGCACCG
CAGCGCCCGGCGGAGGTCGCCCGGCTGGCCGCCGAGGCCGCGGGTGTGCCGTCGAGCGCGGTGCACGCCGTGGTGGTCGC
CGACCTGCCGGTGCTGCCGACCGGCAAACCGGACTACCCCGCGGTGCGGGCACTGGCGGCGCCGGTCGACGACGCACCGC
CGACCACGGATCTGACGACCGTCTTCGCCGAGGTGCTGCACCTCGACCGGGACGGCATCGACCCGGACGCCAGCTTCGTC
GACCTCGGCGGTAACTCGCTGACCTACGTGGCGATGTCGGTCCGGCTGGAACGGGCACTGGGCCGCCTGCCCGCCGACTG
GCAGCGCGTGCCGCTGCGCGATCTGCAGACCCGCGCGGTACCGCCGCGCCGGCGCTGGACGGCCACCCTCGAGACCAGCG
TCGCGCTGCGCGCGGTCGCGATCGTGCTGATCGTCGGGTCGCATGCCGCGCTCTTCGAACTGTGGGGCGGGGCGCATGTG
CTGCTGGGCATCGCGGGGTACAACTTCGGCCGGTTCTGTCTGACCCCCGCTCCCCGCGCGCAGCGGGTCCGGCACCTGCG
CACGACGATCGCCTGGATCGCGGTCCCGTCGGTGGTGTGGGTGGTGATCGCGCTGCTGGTCACCGACGACTACCACCTGT
CGAACCTGTTGCTGGCCAACAAGTTCCTCGGTCCGCACGACAGTATGACCGCCGGGCGGCTGTGGTTCGTCGAGGTGCTG
GTGTGGACCCTCGTCGCGCTGGCGGTGCTGATGGCGGTGCCCGCCGCCGACCGGGCCGAGCGGCGGTGGCCGTTCCTGTT
CGCCGTGGTGTTCCTGGCCGCCGGCCTGGCGTTGCGCTACGACATCCTCGGGCTCGACCTGGGCCGCGACGCGTGGTTCA
CGATGCTGACCTTCTGGTTCTTCGCGGCCGGATGGGCCGCGGCGAAGGCGACTGCACCGTGGCAGCGGGTGGCGGTCACG
ACGGCCCTCGTGGTCGGTTTGTACGGGTACTTCGGGAACACCAACCGCGAGATTCTGGTGCTCGCCGGCTTCCTGCTGCT
GATCTGGCTGCCCGCGCTGCGCTGCCCGCCCGGGTTGACGGTCATCGCGGGCGTCGTCGCCGAGGCGTCGCTGTACACCT
ACCTCACGCACTTCCAGGTGTACGCATTGTTCGAGGGGCGTCCCGCGCTCGGCGTCGCCGCGTCGATCGCGGTGGGTGTG
CTGCTCACCCAAGCGCTCACCCTGCTGCGCCGGCGGTTGCGCGACCGCCGGCCGAGCGTCAGTGCGGCGACGGCGGTTCC
CGCTCGGCGATGA

Upstream 100 bases:

>100_bases
GCGACGGCGCGCCGATCGTCGTGCGCCAGCACAGCCTCGCCCCTCCGCCGGTGGACGCCAAGGTGCGCATCGAGGTGGTG
GGTCACGGAGCGGTGCTGAA

Downstream 100 bases:

>100_bases
GCCCCTCCTGCGCGAGGGTCGCGGCGATGACGCCGTCCGCGCGGACCAGGCTCGCGGTGATCATGCCGCGGCCGCGGGCG
GCGGCCGGTGAGGTGGACTC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 830; Mature: 829

Protein sequence:

>830_residues
MSRLVDCLRAHGDRIAVLTDSERLSYRDLADLVSGSAARLGDTRRLVMLETRNDLPTLVHYLGALAGGHVVLPVPAGRDH
RELTATYDPDVVVDGCGPQIRREGSAHRLHDDLALLLSTSGSTGSPKLVRLSRRNLLSNAHAIADYLDIRETDRAATTLP
MSYCYGLSVVHSHLLRGAALVLTEHSVVDDEFWTLFRRHGGTTFAGVPYTFDLLDRAGFDTMDLPHLRYVTQAGGRLAPE
RVKRYAELGAARGWQLIVMYGATEATARMAYLPPDLAAEHPGCIGVPIPGGSFTIEPLDGWPADDAAGELVYRGDNVMMG
YAHTAADLSRGDEVEALHTGDIARRRPDGLYEVIGRSSRFVKMYGLRIDLQRLETALRARGVPALCTDLDDRLVVAATAP
QRPAEVARLAAEAAGVPSSAVHAVVVADLPVLPTGKPDYPAVRALAAPVDDAPPTTDLTTVFAEVLHLDRDGIDPDASFV
DLGGNSLTYVAMSVRLERALGRLPADWQRVPLRDLQTRAVPPRRRWTATLETSVALRAVAIVLIVGSHAALFELWGGAHV
LLGIAGYNFGRFCLTPAPRAQRVRHLRTTIAWIAVPSVVWVVIALLVTDDYHLSNLLLANKFLGPHDSMTAGRLWFVEVL
VWTLVALAVLMAVPAADRAERRWPFLFAVVFLAAGLALRYDILGLDLGRDAWFTMLTFWFFAAGWAAAKATAPWQRVAVT
TALVVGLYGYFGNTNREILVLAGFLLLIWLPALRCPPGLTVIAGVVAEASLYTYLTHFQVYALFEGRPALGVAASIAVGV
LLTQALTLLRRRLRDRRPSVSAATAVPARR

Sequences:

>Translated_830_residues
MSRLVDCLRAHGDRIAVLTDSERLSYRDLADLVSGSAARLGDTRRLVMLETRNDLPTLVHYLGALAGGHVVLPVPAGRDH
RELTATYDPDVVVDGCGPQIRREGSAHRLHDDLALLLSTSGSTGSPKLVRLSRRNLLSNAHAIADYLDIRETDRAATTLP
MSYCYGLSVVHSHLLRGAALVLTEHSVVDDEFWTLFRRHGGTTFAGVPYTFDLLDRAGFDTMDLPHLRYVTQAGGRLAPE
RVKRYAELGAARGWQLIVMYGATEATARMAYLPPDLAAEHPGCIGVPIPGGSFTIEPLDGWPADDAAGELVYRGDNVMMG
YAHTAADLSRGDEVEALHTGDIARRRPDGLYEVIGRSSRFVKMYGLRIDLQRLETALRARGVPALCTDLDDRLVVAATAP
QRPAEVARLAAEAAGVPSSAVHAVVVADLPVLPTGKPDYPAVRALAAPVDDAPPTTDLTTVFAEVLHLDRDGIDPDASFV
DLGGNSLTYVAMSVRLERALGRLPADWQRVPLRDLQTRAVPPRRRWTATLETSVALRAVAIVLIVGSHAALFELWGGAHV
LLGIAGYNFGRFCLTPAPRAQRVRHLRTTIAWIAVPSVVWVVIALLVTDDYHLSNLLLANKFLGPHDSMTAGRLWFVEVL
VWTLVALAVLMAVPAADRAERRWPFLFAVVFLAAGLALRYDILGLDLGRDAWFTMLTFWFFAAGWAAAKATAPWQRVAVT
TALVVGLYGYFGNTNREILVLAGFLLLIWLPALRCPPGLTVIAGVVAEASLYTYLTHFQVYALFEGRPALGVAASIAVGV
LLTQALTLLRRRLRDRRPSVSAATAVPARR
>Mature_829_residues
SRLVDCLRAHGDRIAVLTDSERLSYRDLADLVSGSAARLGDTRRLVMLETRNDLPTLVHYLGALAGGHVVLPVPAGRDHR
ELTATYDPDVVVDGCGPQIRREGSAHRLHDDLALLLSTSGSTGSPKLVRLSRRNLLSNAHAIADYLDIRETDRAATTLPM
SYCYGLSVVHSHLLRGAALVLTEHSVVDDEFWTLFRRHGGTTFAGVPYTFDLLDRAGFDTMDLPHLRYVTQAGGRLAPER
VKRYAELGAARGWQLIVMYGATEATARMAYLPPDLAAEHPGCIGVPIPGGSFTIEPLDGWPADDAAGELVYRGDNVMMGY
AHTAADLSRGDEVEALHTGDIARRRPDGLYEVIGRSSRFVKMYGLRIDLQRLETALRARGVPALCTDLDDRLVVAATAPQ
RPAEVARLAAEAAGVPSSAVHAVVVADLPVLPTGKPDYPAVRALAAPVDDAPPTTDLTTVFAEVLHLDRDGIDPDASFVD
LGGNSLTYVAMSVRLERALGRLPADWQRVPLRDLQTRAVPPRRRWTATLETSVALRAVAIVLIVGSHAALFELWGGAHVL
LGIAGYNFGRFCLTPAPRAQRVRHLRTTIAWIAVPSVVWVVIALLVTDDYHLSNLLLANKFLGPHDSMTAGRLWFVEVLV
WTLVALAVLMAVPAADRAERRWPFLFAVVFLAAGLALRYDILGLDLGRDAWFTMLTFWFFAAGWAAAKATAPWQRVAVTT
ALVVGLYGYFGNTNREILVLAGFLLLIWLPALRCPPGLTVIAGVVAEASLYTYLTHFQVYALFEGRPALGVAASIAVGVL
LTQALTLLRRRLRDRRPSVSAATAVPARR

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=275, Percent_Identity=27.6363636363636, Blast_Score=81, Evalue=5e-15,
Organism=Escherichia coli, GI1789201, Length=363, Percent_Identity=27.8236914600551, Blast_Score=93, Evalue=7e-20,
Organism=Escherichia coli, GI145693145, Length=277, Percent_Identity=27.4368231046931, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788107, Length=273, Percent_Identity=26.7399267399267, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=261, Percent_Identity=26.4367816091954, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI18859661, Length=367, Percent_Identity=26.9754768392371, Blast_Score=79, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 90256; Mature: 90125

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVDCLRAHGDRIAVLTDSERLSYRDLADLVSGSAARLGDTRRLVMLETRNDLPTLVH
CHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCHHHCCCCEEEEEEECCCCHHHHHH
YLGALAGGHVVLPVPAGRDHRELTATYDPDVVVDGCGPQIRREGSAHRLHDDLALLLSTS
HHHHHCCCCEEEECCCCCCCCEEEEECCCCEEEECCCHHHHCCCCHHHHHHHHEEEEECC
GSTGSPKLVRLSRRNLLSNAHAIADYLDIRETDRAATTLPMSYCYGLSVVHSHLLRGAAL
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHCCEEE
VLTEHSVVDDEFWTLFRRHGGTTFAGVPYTFDLLDRAGFDTMDLPHLRYVTQAGGRLAPE
EEECCCCCCHHHHHHHHHCCCCEEECCCEEHHHHHHCCCCCCCCCHHHHHHHCCCCCCHH
RVKRYAELGAARGWQLIVMYGATEATARMAYLPPDLAAEHPGCIGVPIPGGSFTIEPLDG
HHHHHHHHCCCCCCEEEEEECCCHHHHEEEECCCCCCCCCCCEEEEECCCCCEEEECCCC
WPADDAAGELVYRGDNVMMGYAHTAADLSRGDEVEALHTGDIARRRPDGLYEVIGRSSRF
CCCCCCCCCEEEECCCEEEEECHHHHHHCCCCCEEEEECCCHHHCCCCHHHHHHCCCCCE
VKMYGLRIDLQRLETALRARGVPALCTDLDDRLVVAATAPQRPAEVARLAAEAAGVPSSA
EEEECCEEEHHHHHHHHHHCCCCHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHH
VHAVVVADLPVLPTGKPDYPAVRALAAPVDDAPPTTDLTTVFAEVLHLDRDGIDPDASFV
HEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEE
DLGGNSLTYVAMSVRLERALGRLPADWQRVPLRDLQTRAVPPRRRWTATLETSVALRAVA
ECCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHEEEHHHHHHHHHHHH
IVLIVGSHAALFELWGGAHVLLGIAGYNFGRFCLTPAPRAQRVRHLRTTIAWIAVPSVVW
HHHHCCCCHHHHHHCCCCEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHH
VVIALLVTDDYHLSNLLLANKFLGPHDSMTAGRLWFVEVLVWTLVALAVLMAVPAADRAE
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
RRWPFLFAVVFLAAGLALRYDILGLDLGRDAWFTMLTFWFFAAGWAAAKATAPWQRVAVT
HCCHHHHHHHHHHCCHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
TALVVGLYGYFGNTNREILVLAGFLLLIWLPALRCPPGLTVIAGVVAEASLYTYLTHFQV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEE
YALFEGRPALGVAASIAVGVLLTQALTLLRRRLRDRRPSVSAATAVPARR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SRLVDCLRAHGDRIAVLTDSERLSYRDLADLVSGSAARLGDTRRLVMLETRNDLPTLVH
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCHHHCCCCEEEEEEECCCCHHHHHH
YLGALAGGHVVLPVPAGRDHRELTATYDPDVVVDGCGPQIRREGSAHRLHDDLALLLSTS
HHHHHCCCCEEEECCCCCCCCEEEEECCCCEEEECCCHHHHCCCCHHHHHHHHEEEEECC
GSTGSPKLVRLSRRNLLSNAHAIADYLDIRETDRAATTLPMSYCYGLSVVHSHLLRGAAL
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHCCEEE
VLTEHSVVDDEFWTLFRRHGGTTFAGVPYTFDLLDRAGFDTMDLPHLRYVTQAGGRLAPE
EEECCCCCCHHHHHHHHHCCCCEEECCCEEHHHHHHCCCCCCCCCHHHHHHHCCCCCCHH
RVKRYAELGAARGWQLIVMYGATEATARMAYLPPDLAAEHPGCIGVPIPGGSFTIEPLDG
HHHHHHHHCCCCCCEEEEEECCCHHHHEEEECCCCCCCCCCCEEEEECCCCCEEEECCCC
WPADDAAGELVYRGDNVMMGYAHTAADLSRGDEVEALHTGDIARRRPDGLYEVIGRSSRF
CCCCCCCCCEEEECCCEEEEECHHHHHHCCCCCEEEEECCCHHHCCCCHHHHHHCCCCCE
VKMYGLRIDLQRLETALRARGVPALCTDLDDRLVVAATAPQRPAEVARLAAEAAGVPSSA
EEEECCEEEHHHHHHHHHHCCCCHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHH
VHAVVVADLPVLPTGKPDYPAVRALAAPVDDAPPTTDLTTVFAEVLHLDRDGIDPDASFV
HEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEE
DLGGNSLTYVAMSVRLERALGRLPADWQRVPLRDLQTRAVPPRRRWTATLETSVALRAVA
ECCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHEEEHHHHHHHHHHHH
IVLIVGSHAALFELWGGAHVLLGIAGYNFGRFCLTPAPRAQRVRHLRTTIAWIAVPSVVW
HHHHCCCCHHHHHHCCCCEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHH
VVIALLVTDDYHLSNLLLANKFLGPHDSMTAGRLWFVEVLVWTLVALAVLMAVPAADRAE
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
RRWPFLFAVVFLAAGLALRYDILGLDLGRDAWFTMLTFWFFAAGWAAAKATAPWQRVAVT
HCCHHHHHHHHHHCCHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
TALVVGLYGYFGNTNREILVLAGFLLLIWLPALRCPPGLTVIAGVVAEASLYTYLTHFQV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEE
YALFEGRPALGVAASIAVGVLLTQALTLLRRRLRDRRPSVSAATAVPARR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]