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The map label for this gene is speE [H]

Identifier: 119867965

GI number: 119867965

Start: 2047153

End: 2048715

Strand: Reverse

Name: speE [H]

Synonym: Mkms_1928

Alternate gene names: 119867965

Gene position: 2048715-2047153 (Counterclockwise)

Preceding gene: 119867966

Following gene: 119867963

Centisome position: 35.71

GC content: 71.53

Gene sequence:

>1563_bases
ATGACGACGACCGAACCCGCGCCGCCGGAGTCGGCCACCGGCCGTCCCTCGCCCCGCTGGCGGGCGCTGCTGCTGGCCGC
CGTCGCCGCCTGCGCCGCCTGCGGTCTGGTGTACGAACTGGCCCTGCTGACCCTGTCGTCGACCATCCACGGCGGCGGCA
TCGTCGCGACGTCGCTGATCGTCGCAGGCTACGTGGCGGCACTCGGGGCGGGTGCGCTCGCGGTCAAACCCCTGCTGGGG
CACGCCGCGATCACGTTCGTCGCCGTGGAGACGCTGCTCGGGGTCATCGGCGGCCTCTCCGCCGCCGCGCTGTACGTCAC
GTTCGCGTTCATCGGCGGTTCGACCTGGGTGCTGGTGGTCGGCACGGCGCTGATCGGCGGCCTCGTCGGCGCCGAGGTGC
CGCTGCTGATGACGCTGCTGCAGCGCGGTCGCACCGCCGGGGCCGCCGACAGCGGGCGGGTGCTGGCCAACCTCAACGCC
GCCGACTATTTCGGTGCACTCGTCGGCGGGCTGCTCTGGCCGTTCCTGCTCCTGCCGCACCTCGGGATGATCCGCGGCGC
CGCCGCCACCGGGGTGATCAACCTGGTCGCGGCCGCGGTGGTCGCGGTGTTTCTGCTGCGCCACATCGTCTCGGGCCGGC
AACTTGCCGTCGCGCTGGGTGCGTTGAGCGCCGCGCTGGTGCTGTTGGTGACGCTGCTGATCCGCGCCGACGGGATCGAG
ACCACCACGCGCCAACGGCTTTACAACGACCCGATCGTGGCGTTCGCCCGCTCGCCGTACCAGGAGATCGTCGTCACCAG
ACGGGGTGACGATCTGCGCCTCTACCTCGACGGCGGGCTGCAGTTCTCGACCCGAGACGAGTTCCGGTACACCGAGAGCC
TGGTGCACCCCGCACTCGGGCAGGGTGCGCGATCGGTCCTCGTTCTCGGCGGCGGCGACGGGCTGGCCGCTCGGGAGTTG
TTACGCCAGAACCAGATTCGCGACATCACCCAGGTGGAGCTGGACCCGGCGGTGATCGACCTGGCCCGCACGACGCTGCG
GGAGGCCAACCGCGGCGCCCTCGACGACCCGCGCGTCACCGTCGTGGTCGACGATGCGATGACGTGGCTGCGCGGCGTCG
ACCGGACCTTCGACGCGGTGATCGTCGACCTGCCCGACCCCGACACCCCGGTGCTGGGCCGCCTGTACTCCACGGAGTTC
TACGCGCTGGTCACCCGCGCGCTGGCGCCGGACGGGCTGATGGTGGTGCAGGCCGGCAGCCCCTACTCGACGCCGACGGC
GTTCTGGCGCACGGTGTCGACGATCGAGTCCGCGGGTTTCGCGGTCACCCCGTATCACGTCCACGTGCCGACCTTCGGTG
ACTGGGGTTTCGCGCTGGCCCGTCGCGGTCCGACACCGCCGGCACCCACGGTCCCCGAAGGCGTTGGGCCGCTGCGGTTC
CTCAATCAGGAGGTGCTCGACGCCGCGACGGTGTTCTCCGCGGACGTGGCCCGGCAGCGTTTGGAACCGTCGACGCTGGA
GCACCCCCGCATCGTCGACGACATCCGCAAGGGCTACCGCTAG

Upstream 100 bases:

>100_bases
GAGGCCGACCGGCTGCACCCGTCGGCGATCGCGACTTCGGTCGTGCTGCTGGCGGTGGGAGGGGTGAACGCCGCCGCACT
CTCATGACGACGACAGACTC

Downstream 100 bases:

>100_bases
CCCTCCGGCGCCTGCGGCCCGTTCTCCAGCAGGTTCCGGAATCCGTCCTCGTCGAGCACCGGCACACCGAGATCGATCGC
CTTGTCGTACTTGGAACCCG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MTTTEPAPPESATGRPSPRWRALLLAAVAACAACGLVYELALLTLSSTIHGGGIVATSLIVAGYVAALGAGALAVKPLLG
HAAITFVAVETLLGVIGGLSAAALYVTFAFIGGSTWVLVVGTALIGGLVGAEVPLLMTLLQRGRTAGAADSGRVLANLNA
ADYFGALVGGLLWPFLLLPHLGMIRGAAATGVINLVAAAVVAVFLLRHIVSGRQLAVALGALSAALVLLVTLLIRADGIE
TTTRQRLYNDPIVAFARSPYQEIVVTRRGDDLRLYLDGGLQFSTRDEFRYTESLVHPALGQGARSVLVLGGGDGLAAREL
LRQNQIRDITQVELDPAVIDLARTTLREANRGALDDPRVTVVVDDAMTWLRGVDRTFDAVIVDLPDPDTPVLGRLYSTEF
YALVTRALAPDGLMVVQAGSPYSTPTAFWRTVSTIESAGFAVTPYHVHVPTFGDWGFALARRGPTPPAPTVPEGVGPLRF
LNQEVLDAATVFSADVARQRLEPSTLEHPRIVDDIRKGYR

Sequences:

>Translated_520_residues
MTTTEPAPPESATGRPSPRWRALLLAAVAACAACGLVYELALLTLSSTIHGGGIVATSLIVAGYVAALGAGALAVKPLLG
HAAITFVAVETLLGVIGGLSAAALYVTFAFIGGSTWVLVVGTALIGGLVGAEVPLLMTLLQRGRTAGAADSGRVLANLNA
ADYFGALVGGLLWPFLLLPHLGMIRGAAATGVINLVAAAVVAVFLLRHIVSGRQLAVALGALSAALVLLVTLLIRADGIE
TTTRQRLYNDPIVAFARSPYQEIVVTRRGDDLRLYLDGGLQFSTRDEFRYTESLVHPALGQGARSVLVLGGGDGLAAREL
LRQNQIRDITQVELDPAVIDLARTTLREANRGALDDPRVTVVVDDAMTWLRGVDRTFDAVIVDLPDPDTPVLGRLYSTEF
YALVTRALAPDGLMVVQAGSPYSTPTAFWRTVSTIESAGFAVTPYHVHVPTFGDWGFALARRGPTPPAPTVPEGVGPLRF
LNQEVLDAATVFSADVARQRLEPSTLEHPRIVDDIRKGYR
>Mature_519_residues
TTTEPAPPESATGRPSPRWRALLLAAVAACAACGLVYELALLTLSSTIHGGGIVATSLIVAGYVAALGAGALAVKPLLGH
AAITFVAVETLLGVIGGLSAAALYVTFAFIGGSTWVLVVGTALIGGLVGAEVPLLMTLLQRGRTAGAADSGRVLANLNAA
DYFGALVGGLLWPFLLLPHLGMIRGAAATGVINLVAAAVVAVFLLRHIVSGRQLAVALGALSAALVLLVTLLIRADGIET
TTRQRLYNDPIVAFARSPYQEIVVTRRGDDLRLYLDGGLQFSTRDEFRYTESLVHPALGQGARSVLVLGGGDGLAARELL
RQNQIRDITQVELDPAVIDLARTTLREANRGALDDPRVTVVVDDAMTWLRGVDRTFDAVIVDLPDPDTPVLGRLYSTEFY
ALVTRALAPDGLMVVQAGSPYSTPTAFWRTVSTIESAGFAVTPYHVHVPTFGDWGFALARRGPTPPAPTVPEGVGPLRFL
NQEVLDAATVFSADVARQRLEPSTLEHPRIVDDIRKGYR

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=258, Percent_Identity=29.8449612403101, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786312, Length=173, Percent_Identity=36.4161849710983, Blast_Score=119, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25154106, Length=260, Percent_Identity=32.3076923076923, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI212645616, Length=176, Percent_Identity=32.9545454545455, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323175, Length=262, Percent_Identity=28.6259541984733, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6325326, Length=270, Percent_Identity=28.8888888888889, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24645443, Length=253, Percent_Identity=28.4584980237154, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355941, Length=194, Percent_Identity=28.3505154639175, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 54838; Mature: 54707

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTEPAPPESATGRPSPRWRALLLAAVAACAACGLVYELALLTLSSTIHGGGIVATSLI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VAGYVAALGAGALAVKPLLGHAAITFVAVETLLGVIGGLSAAALYVTFAFIGGSTWVLVV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GTALIGGLVGAEVPLLMTLLQRGRTAGAADSGRVLANLNAADYFGALVGGLLWPFLLLPH
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
LGMIRGAAATGVINLVAAAVVAVFLLRHIVSGRQLAVALGALSAALVLLVTLLIRADGIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TTTRQRLYNDPIVAFARSPYQEIVVTRRGDDLRLYLDGGLQFSTRDEFRYTESLVHPALG
HHHHHHHHCCCHHHHHCCCHHHEEEEECCCEEEEEEECCEEECCCHHHHHHHHHHHHHHC
QGARSVLVLGGGDGLAARELLRQNQIRDITQVELDPAVIDLARTTLREANRGALDDPRVT
CCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEE
VVVDDAMTWLRGVDRTFDAVIVDLPDPDTPVLGRLYSTEFYALVTRALAPDGLMVVQAGS
EEEHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
PYSTPTAFWRTVSTIESAGFAVTPYHVHVPTFGDWGFALARRGPTPPAPTVPEGVGPLRF
CCCCHHHHHHHHHHHHHCCCEECCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHH
LNQEVLDAATVFSADVARQRLEPSTLEHPRIVDDIRKGYR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TTTEPAPPESATGRPSPRWRALLLAAVAACAACGLVYELALLTLSSTIHGGGIVATSLI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VAGYVAALGAGALAVKPLLGHAAITFVAVETLLGVIGGLSAAALYVTFAFIGGSTWVLVV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GTALIGGLVGAEVPLLMTLLQRGRTAGAADSGRVLANLNAADYFGALVGGLLWPFLLLPH
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
LGMIRGAAATGVINLVAAAVVAVFLLRHIVSGRQLAVALGALSAALVLLVTLLIRADGIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TTTRQRLYNDPIVAFARSPYQEIVVTRRGDDLRLYLDGGLQFSTRDEFRYTESLVHPALG
HHHHHHHHCCCHHHHHCCCHHHEEEEECCCEEEEEEECCEEECCCHHHHHHHHHHHHHHC
QGARSVLVLGGGDGLAARELLRQNQIRDITQVELDPAVIDLARTTLREANRGALDDPRVT
CCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEE
VVVDDAMTWLRGVDRTFDAVIVDLPDPDTPVLGRLYSTEFYALVTRALAPDGLMVVQAGS
EEEHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
PYSTPTAFWRTVSTIESAGFAVTPYHVHVPTFGDWGFALARRGPTPPAPTVPEGVGPLRF
CCCCHHHHHHHHHHHHHCCCEECCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHH
LNQEVLDAATVFSADVARQRLEPSTLEHPRIVDDIRKGYR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]