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The map label for this gene is menE [H]

Identifier: 119867573

GI number: 119867573

Start: 1636543

End: 1638090

Strand: Direct

Name: menE [H]

Synonym: Mkms_1525

Alternate gene names: 119867573

Gene position: 1636543-1638090 (Clockwise)

Preceding gene: 119867572

Following gene: 119867574

Centisome position: 28.52

GC content: 65.44

Gene sequence:

>1548_bases
ATGTACCCCGGACGCCACGTCGACGAGCACCCCGACCGTCCCGCGCTGATCATGGCGGGCAGCCGGAAATCGTTGACCTA
CCGCGAGTTCGACGAGCGCGCAAACCGGGTCGCCAACTACTTCCGCGACCTCGGGCTGCGCCGCACCGACCACATCGCGA
TCCTCGCCGAGAACCACCTCGAGATGATCGTGACGATGTCGGCCGCCGAACGGTGCGGCCTGTACTACACCCCGGTCAAC
TCGTTCCTCTCCGTCGACGAGGCCGCCTACATCGTCGACGACTGCGGTGCCCGCCTGCTGGTCACCACCGCCGCCAAATA
CGAAGTGGCCTCGGCGCTTCCGCAGCACTGCCCCAAGGTCGAGCACTGGCTCGTCATCGACAAGGACGACGCACAGGCAC
CGTTCCAGGATTTCGCCCCCGTCATCGAGCAGTACCCGCCGACGCCCGGGCGGGACGAACGGCTGGGCACCCCGATGTTC
TACTCGTCGGGAACCACCGGACGGCCCAAGGCGGTCAAGCGCCAGTTGCCCGATGTCGGCCCACAGGAACAACTCGGCAT
CGAGGAGATGGGCAAGCGCCTGTTCCGGATGCGCGAGGGCATGACGTTCCTGTCGACGGCGCCGCTGTATCACTCCGGCC
CGCAGTCCAGTGTCTCCATCGGACTCCGGCTGGGCGCCACCCACATCGTGATGGAGCGCTTCGACGCCGAACAGTTCCTC
GCGCTCATCGACGAGTTCGGCGTCACCCACACCATGGTGGTGCCCACCATGTTCTCGCGAATCCTCAAACTGCCGAACGA
GATCCGCGACAAGTACGACCACTCCACGCTCGAGGCCGTCGTCCACGGCGCCGCGCCCTGCCCGCAACAGGTCAAGCAGC
AGATGCTCGACTGGTGGGGCCCGGTGATCTACGAGTACTACGGCGGCACCGAGGCCAACGGCACCTGCGGCTGCACGCCA
CAAGAGTGGCTCGCGAATCCCGGCACGGTGGGCAAGGCGTTCTTCGGCGAGATCGTGATCCGCGACGACGACGGCACCGA
ACTCCCGCCGGGCGTGCCCGGGACGATCTGGTTCCGCGGTGGCAACAGTTCCTTCGAGTATCTCAACGATCCGGACAAGA
CGGCCGAAGCGCAGGATCCAAGCGGCACCATGTCGAAGATCGGCGACATCGGATACGTCAACGAGGAAGGCTATCTGTTC
CTCACCGACCGGCAGGCGTTCGTGATCATCTCCGGCGGGGTCAACATCTACCCGCAGGAGATCGAGAACCTCCTGATCAC
CCACCCCGAGGTCATGGACGCCGCCGTATTCGGCGTTCCCGACGAGGATTTCGGCGAGGCGGTCAAGGCGGTGATCCAGC
CCGTCGACCCAGGCGGCGGCACCCCGGACCTCGCACAGCGCCTCCGAGAGTTCTGCCTCGCCCATCTGGCGCGGTTCAAA
TGCCCGAAGTCGTTCGACTTCATCGACGAGATGCCCCGGCTGCCCACCGGCAAGCTGTACAAGCGCAAGCTGCGGGATCC
GTACTGGAAGGAAGTACAGAGTGCGTAG

Upstream 100 bases:

>100_bases
CCCGCATGGGCCAGATGTCCGTGCGTTACGCCAACGGACACGGCGGCTGGGGCATCGGAGAGTTCCTGCGTCCCATCAGC
GCTCGATAGAACCCGCGACG

Downstream 100 bases:

>100_bases
GACGCTCTACACCGAGGAGCACGAGGCGTTCCGAAAGGCGTTCCGGGCCTTCATCGACCACGATGCCGTGCCCCACGTCG
AGGCGTGGGAGAACAGCGGG

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MYPGRHVDEHPDRPALIMAGSRKSLTYREFDERANRVANYFRDLGLRRTDHIAILAENHLEMIVTMSAAERCGLYYTPVN
SFLSVDEAAYIVDDCGARLLVTTAAKYEVASALPQHCPKVEHWLVIDKDDAQAPFQDFAPVIEQYPPTPGRDERLGTPMF
YSSGTTGRPKAVKRQLPDVGPQEQLGIEEMGKRLFRMREGMTFLSTAPLYHSGPQSSVSIGLRLGATHIVMERFDAEQFL
ALIDEFGVTHTMVVPTMFSRILKLPNEIRDKYDHSTLEAVVHGAAPCPQQVKQQMLDWWGPVIYEYYGGTEANGTCGCTP
QEWLANPGTVGKAFFGEIVIRDDDGTELPPGVPGTIWFRGGNSSFEYLNDPDKTAEAQDPSGTMSKIGDIGYVNEEGYLF
LTDRQAFVIISGGVNIYPQEIENLLITHPEVMDAAVFGVPDEDFGEAVKAVIQPVDPGGGTPDLAQRLREFCLAHLARFK
CPKSFDFIDEMPRLPTGKLYKRKLRDPYWKEVQSA

Sequences:

>Translated_515_residues
MYPGRHVDEHPDRPALIMAGSRKSLTYREFDERANRVANYFRDLGLRRTDHIAILAENHLEMIVTMSAAERCGLYYTPVN
SFLSVDEAAYIVDDCGARLLVTTAAKYEVASALPQHCPKVEHWLVIDKDDAQAPFQDFAPVIEQYPPTPGRDERLGTPMF
YSSGTTGRPKAVKRQLPDVGPQEQLGIEEMGKRLFRMREGMTFLSTAPLYHSGPQSSVSIGLRLGATHIVMERFDAEQFL
ALIDEFGVTHTMVVPTMFSRILKLPNEIRDKYDHSTLEAVVHGAAPCPQQVKQQMLDWWGPVIYEYYGGTEANGTCGCTP
QEWLANPGTVGKAFFGEIVIRDDDGTELPPGVPGTIWFRGGNSSFEYLNDPDKTAEAQDPSGTMSKIGDIGYVNEEGYLF
LTDRQAFVIISGGVNIYPQEIENLLITHPEVMDAAVFGVPDEDFGEAVKAVIQPVDPGGGTPDLAQRLREFCLAHLARFK
CPKSFDFIDEMPRLPTGKLYKRKLRDPYWKEVQSA
>Mature_515_residues
MYPGRHVDEHPDRPALIMAGSRKSLTYREFDERANRVANYFRDLGLRRTDHIAILAENHLEMIVTMSAAERCGLYYTPVN
SFLSVDEAAYIVDDCGARLLVTTAAKYEVASALPQHCPKVEHWLVIDKDDAQAPFQDFAPVIEQYPPTPGRDERLGTPMF
YSSGTTGRPKAVKRQLPDVGPQEQLGIEEMGKRLFRMREGMTFLSTAPLYHSGPQSSVSIGLRLGATHIVMERFDAEQFL
ALIDEFGVTHTMVVPTMFSRILKLPNEIRDKYDHSTLEAVVHGAAPCPQQVKQQMLDWWGPVIYEYYGGTEANGTCGCTP
QEWLANPGTVGKAFFGEIVIRDDDGTELPPGVPGTIWFRGGNSSFEYLNDPDKTAEAQDPSGTMSKIGDIGYVNEEGYLF
LTDRQAFVIISGGVNIYPQEIENLLITHPEVMDAAVFGVPDEDFGEAVKAVIQPVDPGGGTPDLAQRLREFCLAHLARFK
CPKSFDFIDEMPRLPTGKLYKRKLRDPYWKEVQSA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=537, Percent_Identity=26.6294227188082, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI38505220, Length=507, Percent_Identity=25.8382642998028, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI115511026, Length=506, Percent_Identity=26.6798418972332, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI122937307, Length=519, Percent_Identity=25.4335260115607, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI157311624, Length=521, Percent_Identity=24.7600767754319, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI157311622, Length=521, Percent_Identity=24.7600767754319, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI42544132, Length=526, Percent_Identity=25.0950570342205, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI58082049, Length=525, Percent_Identity=25.7142857142857, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI28416953, Length=561, Percent_Identity=24.4206773618538, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI187761345, Length=539, Percent_Identity=25.0463821892393, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI187761343, Length=539, Percent_Identity=25.0463821892393, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI40807357, Length=547, Percent_Identity=25.5941499085923, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI38524616, Length=518, Percent_Identity=25.2895752895753, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI221142682, Length=514, Percent_Identity=27.2373540856031, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788107, Length=537, Percent_Identity=27.0018621973929, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI145693145, Length=511, Percent_Identity=25.8317025440313, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790505, Length=549, Percent_Identity=22.040072859745, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786810, Length=519, Percent_Identity=24.6628131021195, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786529, Length=563, Percent_Identity=23.2682060390764, Blast_Score=96, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=524, Percent_Identity=25.381679389313, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=497, Percent_Identity=27.7665995975855, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17558820, Length=495, Percent_Identity=27.6767676767677, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71994703, Length=543, Percent_Identity=25.5985267034991, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71994694, Length=543, Percent_Identity=25.5985267034991, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71994690, Length=543, Percent_Identity=25.5985267034991, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=515, Percent_Identity=24.6601941747573, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=360, Percent_Identity=29.1666666666667, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560308, Length=541, Percent_Identity=26.4325323475046, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=532, Percent_Identity=24.2481203007519, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17551278, Length=563, Percent_Identity=24.5115452930728, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115534440, Length=543, Percent_Identity=22.6519337016575, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560140, Length=357, Percent_Identity=25.4901960784314, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=391, Percent_Identity=25.0639386189258, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI25141359, Length=497, Percent_Identity=20.9255533199195, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=538, Percent_Identity=24.7211895910781, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6319264, Length=572, Percent_Identity=24.6503496503496, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323182, Length=563, Percent_Identity=23.4458259325044, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=26.1859582542694, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24581924, Length=538, Percent_Identity=26.5799256505576, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI18859661, Length=488, Percent_Identity=25.6147540983607, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI281365686, Length=501, Percent_Identity=25.1497005988024, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922652, Length=514, Percent_Identity=22.9571984435798, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21356947, Length=493, Percent_Identity=25.9634888438134, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24648255, Length=478, Percent_Identity=24.8953974895398, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648253, Length=507, Percent_Identity=24.2603550295858, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21358303, Length=495, Percent_Identity=24.8484848484848, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356441, Length=524, Percent_Identity=23.6641221374046, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24656500, Length=509, Percent_Identity=23.3791748526523, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648260, Length=512, Percent_Identity=23.046875, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21355225, Length=536, Percent_Identity=22.5746268656716, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648257, Length=530, Percent_Identity=23.0188679245283, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653035, Length=496, Percent_Identity=22.5806451612903, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI161076582, Length=508, Percent_Identity=21.8503937007874, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI20130357, Length=506, Percent_Identity=23.9130434782609, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 57520; Mature: 57520

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPGRHVDEHPDRPALIMAGSRKSLTYREFDERANRVANYFRDLGLRRTDHIAILAENHL
CCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCE
EMIVTMSAAERCGLYYTPVNSFLSVDEAAYIVDDCGARLLVTTAAKYEVASALPQHCPKV
EEEEEHHHHHHCCEEECCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCC
EHWLVIDKDDAQAPFQDFAPVIEQYPPTPGRDERLGTPMFYSSGTTGRPKAVKRQLPDVG
CEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHCCCCC
PQEQLGIEEMGKRLFRMREGMTFLSTAPLYHSGPQSSVSIGLRLGATHIVMERFDAEQFL
CHHHCCHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCEEEEEEECHHHHHHHHHCHHHHH
ALIDEFGVTHTMVVPTMFSRILKLPNEIRDKYDHSTLEAVVHGAAPCPQQVKQQMLDWWG
HHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHH
PVIYEYYGGTEANGTCGCTPQEWLANPGTVGKAFFGEIVIRDDDGTELPPGVPGTIWFRG
HHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCHHHCCEEEEECCCCCCCCCCCCCEEEEEC
GNSSFEYLNDPDKTAEAQDPSGTMSKIGDIGYVNEEGYLFLTDRQAFVIISGGVNIYPQE
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEECCCCEEEEECCCEEEEEECCCEECHHH
IENLLITHPEVMDAAVFGVPDEDFGEAVKAVIQPVDPGGGTPDLAQRLREFCLAHLARFK
HHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
CPKSFDFIDEMPRLPTGKLYKRKLRDPYWKEVQSA
CCCCCCHHHHCCCCCCCHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MYPGRHVDEHPDRPALIMAGSRKSLTYREFDERANRVANYFRDLGLRRTDHIAILAENHL
CCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCE
EMIVTMSAAERCGLYYTPVNSFLSVDEAAYIVDDCGARLLVTTAAKYEVASALPQHCPKV
EEEEEHHHHHHCCEEECCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCC
EHWLVIDKDDAQAPFQDFAPVIEQYPPTPGRDERLGTPMFYSSGTTGRPKAVKRQLPDVG
CEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHCCCCC
PQEQLGIEEMGKRLFRMREGMTFLSTAPLYHSGPQSSVSIGLRLGATHIVMERFDAEQFL
CHHHCCHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCEEEEEEECHHHHHHHHHCHHHHH
ALIDEFGVTHTMVVPTMFSRILKLPNEIRDKYDHSTLEAVVHGAAPCPQQVKQQMLDWWG
HHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHH
PVIYEYYGGTEANGTCGCTPQEWLANPGTVGKAFFGEIVIRDDDGTELPPGVPGTIWFRG
HHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCHHHCCEEEEECCCCCCCCCCCCCEEEEEC
GNSSFEYLNDPDKTAEAQDPSGTMSKIGDIGYVNEEGYLFLTDRQAFVIISGGVNIYPQE
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEECCCCEEEEECCCEEEEEECCCEECHHH
IENLLITHPEVMDAAVFGVPDEDFGEAVKAVIQPVDPGGGTPDLAQRLREFCLAHLARFK
HHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
CPKSFDFIDEMPRLPTGKLYKRKLRDPYWKEVQSA
CCCCCCHHHHCCCCCCCHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA