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The map label for this gene is mcrA [H]

Identifier: 119867485

GI number: 119867485

Start: 1543663

End: 1545051

Strand: Direct

Name: mcrA [H]

Synonym: Mkms_1435

Alternate gene names: 119867485

Gene position: 1543663-1545051 (Clockwise)

Preceding gene: 119867484

Following gene: 119867492

Centisome position: 26.91

GC content: 71.78

Gene sequence:

>1389_bases
ATGACTCTGACCGATACAGCCCGCGACGGCATCGCCGACCAGGTGGCCGCGATCCGGGAGCACGTCTCGGCACCGGTGGC
CATGCCGGGGGAGGCGGGTTACGAGCGGTGCACACCGTGGAACGTCGCCGCCGACGTGCGCCCCGCCGCGGTGGTGCTGG
CCACGTCGGCCCCCGATGTCGCCGACACCGTCCGGTTCGCCGCGGCCCGTGGTCTGCGGGTGACGGTGCAGGCCACCGGG
CACGGTGCGACCGGGGTGGGCGCGGACACGATCCTGATCGTCACGTCGGGGATGTCCACGTGTGCCGTCGACGCGATGAA
CCGCATCGCGCGCGTCGGCGCGGGCGTGCGGTGGCAGCAGGTGCTCGACGCAGCGTGCCCGTACGGGCTGGCGCCGGTGG
TCGGCTCGGCGCCGGGGGTGGGTGTCGTGGGCTTTCTGACCGGTGGGGGCATCGGACCGCTGGTGCGCACCCTCGGCGCG
TCATCGGATTACGTTCGTGCTTTCGAATTGGTCACCGGCACGGGGGAAGTGCTGCGGGTGACACCCGATGACCATGCCGA
CCTGTTCTGGGGTCTGCGGGGCGGTAAGGCAACGCTGGGCATCGTCACCTCGGTGGAGTTCGATCTGCTCCCGATCCCCG
AATTCTACGGTGGCACTGTCTACTTCGACGGTGCCGATGCGCCCGCGGTGGTGCGCGCGTGGGCGGACTGGTGTGGTGGC
CTGCCGGAGACGGTGAACACCTCGATTGCGCTGCAACAGCTTCCGCCGCTGCCGGGCGTTCCTGAACCGCTTGCCGGTCG
GTTGACGGTCGCGGTGCGCTTCGCCGCCGTCGGTGACTTCGCCGAAGCCGAACGCATCCTCGCGCCGATGCGGGCAGTGG
CGGCGCCGCTGATGGACACGGTTGGGGTGCTGCCGTACGCGGCCGTCGGTGCGGTGCACGCCGATCCGGTCGACCCGATG
CCGGTGTACGAGGATCACACGCTACTACACAGTTTCGACCCCGCGGCCGCCGAGGCGCTGCTGGGCGTGGCGGGGCCGGG
TTCGGAGTCGGTGCAGGTGATCGTGGAGGTGCGCCATCTTGGCGGGGCGCTGGCGCGTGAGCCGCAGCACCGCAGCGCGC
TGTGCCACCGCGATGCGGCGTTCGCGCTGACCACCATCGGGGCGCTGGTGCCGCCGATCGTCGAGGTGGTGCCGGTGCAC
GCCGCGGCGGTGATGAGCGCGCTGGGGCGCTGGTCGACCGGTGGGCAGCTGCCGAACTTCGCACCGTCGATGGAGCCCGG
CCGCGCCGCCCGGGTTTACACCGAGGACGCCAGGCACTGGCTGGCAGCGCTGGCGGAGCGCTACGACCCGTCCGGGGTCT
TCCGGACGGGCCAGGTCGTCCGCAGCTGA

Upstream 100 bases:

>100_bases
AGGGCTTGGAGCCGCCTTGGCGTGGCCGTCCAAGCCGACGCCAAGAGCGGTGATGCACGCTGTGGGCCGAAATCGAGCAC
AGCGCAGTGAAAGGACTTCG

Downstream 100 bases:

>100_bases
GGCCTTCCGCGAGCGGCGAGTCAAGTTCGGGACGTGCTGCTCGCGGCCGGGCTTGGGAGACAAAGCGCTGGTAGACGCCC
GATTTGGAATACCAAGCCGT

Product: FAD linked oxidase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTLTDTARDGIADQVAAIREHVSAPVAMPGEAGYERCTPWNVAADVRPAAVVLATSAPDVADTVRFAAARGLRVTVQATG
HGATGVGADTILIVTSGMSTCAVDAMNRIARVGAGVRWQQVLDAACPYGLAPVVGSAPGVGVVGFLTGGGIGPLVRTLGA
SSDYVRAFELVTGTGEVLRVTPDDHADLFWGLRGGKATLGIVTSVEFDLLPIPEFYGGTVYFDGADAPAVVRAWADWCGG
LPETVNTSIALQQLPPLPGVPEPLAGRLTVAVRFAAVGDFAEAERILAPMRAVAAPLMDTVGVLPYAAVGAVHADPVDPM
PVYEDHTLLHSFDPAAAEALLGVAGPGSESVQVIVEVRHLGGALAREPQHRSALCHRDAAFALTTIGALVPPIVEVVPVH
AAAVMSALGRWSTGGQLPNFAPSMEPGRAARVYTEDARHWLAALAERYDPSGVFRTGQVVRS

Sequences:

>Translated_462_residues
MTLTDTARDGIADQVAAIREHVSAPVAMPGEAGYERCTPWNVAADVRPAAVVLATSAPDVADTVRFAAARGLRVTVQATG
HGATGVGADTILIVTSGMSTCAVDAMNRIARVGAGVRWQQVLDAACPYGLAPVVGSAPGVGVVGFLTGGGIGPLVRTLGA
SSDYVRAFELVTGTGEVLRVTPDDHADLFWGLRGGKATLGIVTSVEFDLLPIPEFYGGTVYFDGADAPAVVRAWADWCGG
LPETVNTSIALQQLPPLPGVPEPLAGRLTVAVRFAAVGDFAEAERILAPMRAVAAPLMDTVGVLPYAAVGAVHADPVDPM
PVYEDHTLLHSFDPAAAEALLGVAGPGSESVQVIVEVRHLGGALAREPQHRSALCHRDAAFALTTIGALVPPIVEVVPVH
AAAVMSALGRWSTGGQLPNFAPSMEPGRAARVYTEDARHWLAALAERYDPSGVFRTGQVVRS
>Mature_461_residues
TLTDTARDGIADQVAAIREHVSAPVAMPGEAGYERCTPWNVAADVRPAAVVLATSAPDVADTVRFAAARGLRVTVQATGH
GATGVGADTILIVTSGMSTCAVDAMNRIARVGAGVRWQQVLDAACPYGLAPVVGSAPGVGVVGFLTGGGIGPLVRTLGAS
SDYVRAFELVTGTGEVLRVTPDDHADLFWGLRGGKATLGIVTSVEFDLLPIPEFYGGTVYFDGADAPAVVRAWADWCGGL
PETVNTSIALQQLPPLPGVPEPLAGRLTVAVRFAAVGDFAEAERILAPMRAVAAPLMDTVGVLPYAAVGAVHADPVDPMP
VYEDHTLLHSFDPAAAEALLGVAGPGSESVQVIVEVRHLGGALAREPQHRSALCHRDAAFALTTIGALVPPIVEVVPVHA
AAVMSALGRWSTGGQLPNFAPSMEPGRAARVYTEDARHWLAALAERYDPSGVFRTGQVVRS

Specific function: Involved in mitomycin resistance; oxidizes reduced form of mitomycins [H]

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012951
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR006093 [H]

Pfam domain/function: PF08031 BBE; PF01565 FAD_binding_4 [H]

EC number: NA

Molecular weight: Translated: 47781; Mature: 47650

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00862 OX2_COVAL_FAD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTDTARDGIADQVAAIREHVSAPVAMPGEAGYERCTPWNVAADVRPAAVVLATSAPDV
CCCCCCHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCH
ADTVRFAAARGLRVTVQATGHGATGVGADTILIVTSGMSTCAVDAMNRIARVGAGVRWQQ
HHHHHHHHHCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHH
VLDAACPYGLAPVVGSAPGVGVVGFLTGGGIGPLVRTLGASSDYVRAFELVTGTGEVLRV
HHHHHCCCCCCHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCCHHEEEEEEECCCCEEEE
TPDDHADLFWGLRGGKATLGIVTSVEFDLLPIPEFYGGTVYFDGADAPAVVRAWADWCGG
CCCCCCCEEEECCCCCEEEEEEEECEECEEECCHHCCCEEEECCCCCHHHHHHHHHHHCC
LPETVNTSIALQQLPPLPGVPEPLAGRLTVAVRFAAVGDFAEAERILAPMRAVAAPLMDT
CCCHHCCCEEHHHCCCCCCCCCCCCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHH
VGVLPYAAVGAVHADPVDPMPVYEDHTLLHSFDPAAAEALLGVAGPGSESVQVIVEVRHL
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEHHH
GGALAREPQHRSALCHRDAAFALTTIGALVPPIVEVVPVHAAAVMSALGRWSTGGQLPNF
CCHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
APSMEPGRAARVYTEDARHWLAALAERYDPSGVFRTGQVVRS
CCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEECCHHHCC
>Mature Secondary Structure 
TLTDTARDGIADQVAAIREHVSAPVAMPGEAGYERCTPWNVAADVRPAAVVLATSAPDV
CCCCCHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCH
ADTVRFAAARGLRVTVQATGHGATGVGADTILIVTSGMSTCAVDAMNRIARVGAGVRWQQ
HHHHHHHHHCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHH
VLDAACPYGLAPVVGSAPGVGVVGFLTGGGIGPLVRTLGASSDYVRAFELVTGTGEVLRV
HHHHHCCCCCCHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCCHHEEEEEEECCCCEEEE
TPDDHADLFWGLRGGKATLGIVTSVEFDLLPIPEFYGGTVYFDGADAPAVVRAWADWCGG
CCCCCCCEEEECCCCCEEEEEEEECEECEEECCHHCCCEEEECCCCCHHHHHHHHHHHCC
LPETVNTSIALQQLPPLPGVPEPLAGRLTVAVRFAAVGDFAEAERILAPMRAVAAPLMDT
CCCHHCCCEEHHHCCCCCCCCCCCCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHH
VGVLPYAAVGAVHADPVDPMPVYEDHTLLHSFDPAAAEALLGVAGPGSESVQVIVEVRHL
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEHHH
GGALAREPQHRSALCHRDAAFALTTIGALVPPIVEVVPVHAAAVMSALGRWSTGGQLPNF
CCHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
APSMEPGRAARVYTEDARHWLAALAERYDPSGVFRTGQVVRS
CCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517396 [H]