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The map label for this gene is menF [H]

Identifier: 119867455

GI number: 119867455

Start: 1505423

End: 1506520

Strand: Reverse

Name: menF [H]

Synonym: Mkms_1405

Alternate gene names: 119867455

Gene position: 1506520-1505423 (Counterclockwise)

Preceding gene: 119867456

Following gene: 119867454

Centisome position: 26.26

GC content: 72.22

Gene sequence:

>1098_bases
GTGACCGCCGAGCCGAGCTTCGTCCTCACCGGCGACGACGGTGTCGTACTGGCCGACGGCGTCCACACGCCGTACCCCAC
GCTGGGCGACGCGTGCGCCGCGCTGGCCGCAGGCGATACCCCGATGATCTTGGGCGCCTTGCCTTTCGACCTGACCGCAC
CGACGGCACTGTTCCGCCCGCAGCGCGTGCGCTTCCTCGATGCGCTGCCGGACTGGCCGGCCGTCGGCCTGCCACGTGTA
CGGATCGCCGAGTTCCGGCCCGCACCCCAGGAGCACCGCGCACGCGTCGCGTCGGCGGTGCGCGCGCTCAACGACCCCGC
CACCGGACTGCACAAAGTCGTACTCGCGCGGGCGCTGCACCTGACCGCCGACGGACCACTCGACGCGCGAACCGTGCTGC
GGCGCCTGGTCGCCGACGATGCGAGCGCCAACGCGTATCTGGCCGATCTCACCGCAGCCGGAGACCACTACCGCGGTGCG
GCCCTGATCGGGGCCAGCCCGGAACTGCTGGTGGCCCGGCGCGGTGAGGTGGTGACGTGCCAACCGTTCGCCGGTTCGGC
GCCGCGGTTCGCCGACCCCGCGGCCGACCGGGCCAGCGGCGCCGCCCTCGCCGACTCGGCCAAGAACCGGCACGAACACG
CCATCGTGATCGACGAGGTGCGCAAGGCCCTCGACCCGCTGTGCGTGGATCTGCAGATCGCGCCGACACCGCACCTCAGC
GCGACGGCCGCCGTCTGGCACCTGAGCACCCAGATCACCGGCCGACTGCGCGAAACAAACACCACCGCACTGGATCTCGC
GGTCGCGCTGCACCCGACCCCGGCCGTCGGCGGCGTCCCGACCGACTTGGCCGCGGAGTTGATCAAACGCCTCGAGGGCG
ACCGCGGCTTCTACGCCGGCGCCGTCGGCTGGTGCGACGCGGCGGGTGACGGCCGCTGGGTGGTGTCCATCCGGTGCGCC
CAACTGGCCGCCGATCGGCTCGCCGCCACCGCCCACTCCGGGGGCGGCATCGTCGCCGAATCCGATCCGGACGACGAAGT
CGCCGAGACCACAACGAAATTCACGACCATCCTGTCCGCACTGGGGGTAACGCAATGA

Upstream 100 bases:

>100_bases
CGATGGTCGCGGCGTCGATCGCGGTGTGTGGTTGGGAGCACGGGGTGCGCCAGATCAGCGCGTTGGCACTGACCGGTTAT
CCGCATCCGTGCCTGCCGAC

Downstream 100 bases:

>100_bases
CTGTGTCCGTCCGCCGGGTCGAACCCGGTGACGAGGTGGAGCTCACCGCGATGGTGCACGAGCTGGCCGCGTTCGAACGC
GCTTCCGACGAGTGCACCGT

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTAEPSFVLTGDDGVVLADGVHTPYPTLGDACAALAAGDTPMILGALPFDLTAPTALFRPQRVRFLDALPDWPAVGLPRV
RIAEFRPAPQEHRARVASAVRALNDPATGLHKVVLARALHLTADGPLDARTVLRRLVADDASANAYLADLTAAGDHYRGA
ALIGASPELLVARRGEVVTCQPFAGSAPRFADPAADRASGAALADSAKNRHEHAIVIDEVRKALDPLCVDLQIAPTPHLS
ATAAVWHLSTQITGRLRETNTTALDLAVALHPTPAVGGVPTDLAAELIKRLEGDRGFYAGAVGWCDAAGDGRWVVSIRCA
QLAADRLAATAHSGGGIVAESDPDDEVAETTTKFTTILSALGVTQ

Sequences:

>Translated_365_residues
MTAEPSFVLTGDDGVVLADGVHTPYPTLGDACAALAAGDTPMILGALPFDLTAPTALFRPQRVRFLDALPDWPAVGLPRV
RIAEFRPAPQEHRARVASAVRALNDPATGLHKVVLARALHLTADGPLDARTVLRRLVADDASANAYLADLTAAGDHYRGA
ALIGASPELLVARRGEVVTCQPFAGSAPRFADPAADRASGAALADSAKNRHEHAIVIDEVRKALDPLCVDLQIAPTPHLS
ATAAVWHLSTQITGRLRETNTTALDLAVALHPTPAVGGVPTDLAAELIKRLEGDRGFYAGAVGWCDAAGDGRWVVSIRCA
QLAADRLAATAHSGGGIVAESDPDDEVAETTTKFTTILSALGVTQ
>Mature_364_residues
TAEPSFVLTGDDGVVLADGVHTPYPTLGDACAALAAGDTPMILGALPFDLTAPTALFRPQRVRFLDALPDWPAVGLPRVR
IAEFRPAPQEHRARVASAVRALNDPATGLHKVVLARALHLTADGPLDARTVLRRLVADDASANAYLADLTAAGDHYRGAA
LIGASPELLVARRGEVVTCQPFAGSAPRFADPAADRASGAALADSAKNRHEHAIVIDEVRKALDPLCVDLQIAPTPHLSA
TAAVWHLSTQITGRLRETNTTALDLAVALHPTPAVGGVPTDLAAELIKRLEGDRGFYAGAVGWCDAAGDGRWVVSIRCAQ
LAADRLAATAHSGGGIVAESDPDDEVAETTTKFTTILSALGVTQ

Specific function: Might be involved in the biosynthesis of menaquinone. Converts chorismate to isochorismate [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=379, Percent_Identity=34.0369393139842, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1788114, Length=254, Percent_Identity=28.740157480315, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082077, Length=251, Percent_Identity=30.6772908366534, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787518, Length=318, Percent_Identity=28.3018867924528, Blast_Score=79, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320935, Length=210, Percent_Identity=36.1904761904762, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 38021; Mature: 37890

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEPSFVLTGDDGVVLADGVHTPYPTLGDACAALAAGDTPMILGALPFDLTAPTALFRP
CCCCCCEEEECCCCEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHCCC
QRVRFLDALPDWPAVGLPRVRIAEFRPAPQEHRARVASAVRALNDPATGLHKVVLARALH
HHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LTADGPLDARTVLRRLVADDASANAYLADLTAAGDHYRGAALIGASPELLVARRGEVVTC
CCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEECCCCCEEEECCCCEEEE
QPFAGSAPRFADPAADRASGAALADSAKNRHEHAIVIDEVRKALDPLCVDLQIAPTPHLS
CCCCCCCCCCCCCCCHHCCCCHHHHHHCCCCCCEEEHHHHHHHHCCEEEEEEECCCCCCC
ATAAVWHLSTQITGRLRETNTTALDLAVALHPTPAVGGVPTDLAAELIKRLEGDRGFYAG
HHHHEEEEHHHHHCCHHCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEC
AVGWCDAAGDGRWVVSIRCAQLAADRLAATAHSGGGIVAESDPDDEVAETTTKFTTILSA
CCEEECCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
LGVTQ
HCCCC
>Mature Secondary Structure 
TAEPSFVLTGDDGVVLADGVHTPYPTLGDACAALAAGDTPMILGALPFDLTAPTALFRP
CCCCCEEEECCCCEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHCCC
QRVRFLDALPDWPAVGLPRVRIAEFRPAPQEHRARVASAVRALNDPATGLHKVVLARALH
HHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LTADGPLDARTVLRRLVADDASANAYLADLTAAGDHYRGAALIGASPELLVARRGEVVTC
CCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEECCCCCEEEECCCCEEEE
QPFAGSAPRFADPAADRASGAALADSAKNRHEHAIVIDEVRKALDPLCVDLQIAPTPHLS
CCCCCCCCCCCCCCCHHCCCCHHHHHHCCCCCCEEEHHHHHHHHCCEEEEEEECCCCCCC
ATAAVWHLSTQITGRLRETNTTALDLAVALHPTPAVGGVPTDLAAELIKRLEGDRGFYAG
HHHHEEEEHHHHHCCHHCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEC
AVGWCDAAGDGRWVVSIRCAQLAADRLAATAHSGGGIVAESDPDDEVAETTTKFTTILSA
CCEEECCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
LGVTQ
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]