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The map label for this gene is cofE [H]

Identifier: 119867390

GI number: 119867390

Start: 1434362

End: 1435729

Strand: Reverse

Name: cofE [H]

Synonym: Mkms_1340

Alternate gene names: 119867390

Gene position: 1435729-1434362 (Counterclockwise)

Preceding gene: 119867391

Following gene: 119867389

Centisome position: 25.02

GC content: 72.22

Gene sequence:

>1368_bases
GTGACCGCACACGGCGGCTCCGCCGCGCCGACCGACCACGGCTCGGCCGCGGCGGTGGAGATCCTGCCGGTGCCGGGGTT
GCCGGAGTTCCGGCCCGGTGACGACCTGGCCGCCGCACTGGCAGCCGCCGCACCGTGGCTGCGCGACGGCGACGTCGTGG
TGGTCACCAGCAAGGTGGTGTCCAAGTGCGAGGGCCGCATCGTCGCCGCACCGGAGGACCCCGAGGAGCGGGATGCGTTG
CGCCGCAAACTCGTCGACGACGAAGCCGTGCGGGTCCTCGCCCGCAAGGGGCGCACGCTGATCACCGAGAACGCCATCGG
GCTCATCCAGGCGGCGGCCGGAGTCGACGGTTCCAACGTCGACACGACCGAACTCGCCCTGCTGCCGGTCGACCCCGACG
GAAGCGCCGCGCACCTGCGGACCGGCCTCGCCGAACGACTCGGTGTCACGGTGGGAATCGTCATCACCGACACCATGGGA
CGCGCGTGGCGCAACGGTCAGATCGATGCGGCGATCGGCGCCGCCGGCCTGGCCGTGCTGCACGGATACGCCGGCGCGCG
TGACGCGCACGGCAACGAACTGCTGGTCACCGAGGTGGCCGTCGCCGACGAGATCGCCGCCGCCGCGGACCTGGTCAAGG
GCAAGCTCACCGGCATACCGGTGGCCGTGGTGCGCGGATTGACGTTGGCGGACAACGGATCAACGGCGCATGATCTGCTG
CGCCCCGGCGAGGACGATTTGTTCTGGCTGGGCACCGAGGAGGCCATGGCGCAGGGCCGCGCCGAGGCTCAGCTGCTCCG
GCGTTCGATACGCCGGTTCAGTGCCGACCCCGTGGCTCCCGAGATGATCGAGGCCGCCGTCGCCGAGGCGCTCACGGCTC
CCGCGCCGCACCACACGCGGCCCGTCCGCTTCGTCTGGGTGGGCGACCACGCCACCCGCATCCGTCTGCTCGACCGGATG
AAGGACCGCTGGCGCGACGATCTACTCGCCGACGGGCTTTCGGCGGACGCCGTCGAACGCCGGCTGGCGCGTGGCCAGAT
CCTCTACGACGCACCGGAACTCGTCATCCCGTTCCTGGTGCCCGACGGCGCGCACGACTATCCGGACGACACCCGCACCG
CGGCCGAGCACACCATGTTCACCGTGGCGGTGGGCGCCGCCGTCCAGGCGTTCCTCGTCGCGCTGGCGGTGCGCGAGGTC
GGCAGCTGCTGGGTCGGCTCGACGATCTTCGCCGCGGATGTGGTGCGTGCGGAACTGGACCTGCCCGCCGAGTGGGAACC
GTTGGGCGCCGTCGCGGTCGGCCACCCCGCCGAGCCGCAGGGCCCCCGTGATCCTGTTCTGCCAGAAGGGCTCCTGGTGC
GCCGGTGA

Upstream 100 bases:

>100_bases
TCGACGGCGTGCAGGTCGAGGCGGTGCCGCTGTTGATGACCGATCCGGCGACCACCGCCGAGATGGTCCGCGCCGGTGTG
CGCCTGGCCGGGGTGACGCT

Downstream 100 bases:

>100_bases
GCCTGCACGCATCGGCGGTCGACATCCTGACCGACTGGGTCGCCCCCGACCCGGCGCAGGATTCGTTGCGTCACGCCGTG
ATCGCATTCCTCGCCGCACG

Product: F420-0--gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTAHGGSAAPTDHGSAAAVEILPVPGLPEFRPGDDLAAALAAAAPWLRDGDVVVVTSKVVSKCEGRIVAAPEDPEERDAL
RRKLVDDEAVRVLARKGRTLITENAIGLIQAAAGVDGSNVDTTELALLPVDPDGSAAHLRTGLAERLGVTVGIVITDTMG
RAWRNGQIDAAIGAAGLAVLHGYAGARDAHGNELLVTEVAVADEIAAAADLVKGKLTGIPVAVVRGLTLADNGSTAHDLL
RPGEDDLFWLGTEEAMAQGRAEAQLLRRSIRRFSADPVAPEMIEAAVAEALTAPAPHHTRPVRFVWVGDHATRIRLLDRM
KDRWRDDLLADGLSADAVERRLARGQILYDAPELVIPFLVPDGAHDYPDDTRTAAEHTMFTVAVGAAVQAFLVALAVREV
GSCWVGSTIFAADVVRAELDLPAEWEPLGAVAVGHPAEPQGPRDPVLPEGLLVRR

Sequences:

>Translated_455_residues
MTAHGGSAAPTDHGSAAAVEILPVPGLPEFRPGDDLAAALAAAAPWLRDGDVVVVTSKVVSKCEGRIVAAPEDPEERDAL
RRKLVDDEAVRVLARKGRTLITENAIGLIQAAAGVDGSNVDTTELALLPVDPDGSAAHLRTGLAERLGVTVGIVITDTMG
RAWRNGQIDAAIGAAGLAVLHGYAGARDAHGNELLVTEVAVADEIAAAADLVKGKLTGIPVAVVRGLTLADNGSTAHDLL
RPGEDDLFWLGTEEAMAQGRAEAQLLRRSIRRFSADPVAPEMIEAAVAEALTAPAPHHTRPVRFVWVGDHATRIRLLDRM
KDRWRDDLLADGLSADAVERRLARGQILYDAPELVIPFLVPDGAHDYPDDTRTAAEHTMFTVAVGAAVQAFLVALAVREV
GSCWVGSTIFAADVVRAELDLPAEWEPLGAVAVGHPAEPQGPRDPVLPEGLLVRR
>Mature_454_residues
TAHGGSAAPTDHGSAAAVEILPVPGLPEFRPGDDLAAALAAAAPWLRDGDVVVVTSKVVSKCEGRIVAAPEDPEERDALR
RKLVDDEAVRVLARKGRTLITENAIGLIQAAAGVDGSNVDTTELALLPVDPDGSAAHLRTGLAERLGVTVGIVITDTMGR
AWRNGQIDAAIGAAGLAVLHGYAGARDAHGNELLVTEVAVADEIAAAADLVKGKLTGIPVAVVRGLTLADNGSTAHDLLR
PGEDDLFWLGTEEAMAQGRAEAQLLRRSIRRFSADPVAPEMIEAAVAEALTAPAPHHTRPVRFVWVGDHATRIRLLDRMK
DRWRDDLLADGLSADAVERRLARGQILYDAPELVIPFLVPDGAHDYPDDTRTAAEHTMFTVAVGAAVQAFLVALAVREVG
SCWVGSTIFAADVVRAELDLPAEWEPLGAVAVGHPAEPQGPRDPVLPEGLLVRR

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225
- InterPro:   IPR019943
- InterPro:   IPR000415 [H]

Pfam domain/function: PF01996 F420_ligase; PF00881 Nitroreductase [H]

EC number: NA

Molecular weight: Translated: 47921; Mature: 47790

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHGGSAAPTDHGSAAAVEILPVPGLPEFRPGDDLAAALAAAAPWLRDGDVVVVTSKVV
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHH
SKCEGRIVAAPEDPEERDALRRKLVDDEAVRVLARKGRTLITENAIGLIQAAAGVDGSNV
HHHCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCC
DTTELALLPVDPDGSAAHLRTGLAERLGVTVGIVITDTMGRAWRNGQIDAAIGAAGLAVL
CCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHCCCCCCHHHHHHHHHHH
HGYAGARDAHGNELLVTEVAVADEIAAAADLVKGKLTGIPVAVVRGLTLADNGSTAHDLL
HCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEECCCCCHHHHH
RPGEDDLFWLGTEEAMAQGRAEAQLLRRSIRRFSADPVAPEMIEAAVAEALTAPAPHHTR
CCCCCCEEEEECHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
PVRFVWVGDHATRIRLLDRMKDRWRDDLLADGLSADAVERRLARGQILYDAPELVIPFLV
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCEEEEEEE
PDGAHDYPDDTRTAAEHTMFTVAVGAAVQAFLVALAVREVGSCWVGSTIFAADVVRAELD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCC
LPAEWEPLGAVAVGHPAEPQGPRDPVLPEGLLVRR
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAHGGSAAPTDHGSAAAVEILPVPGLPEFRPGDDLAAALAAAAPWLRDGDVVVVTSKVV
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHH
SKCEGRIVAAPEDPEERDALRRKLVDDEAVRVLARKGRTLITENAIGLIQAAAGVDGSNV
HHHCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCC
DTTELALLPVDPDGSAAHLRTGLAERLGVTVGIVITDTMGRAWRNGQIDAAIGAAGLAVL
CCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHCCCCCCHHHHHHHHHHH
HGYAGARDAHGNELLVTEVAVADEIAAAADLVKGKLTGIPVAVVRGLTLADNGSTAHDLL
HCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEECCCCCHHHHH
RPGEDDLFWLGTEEAMAQGRAEAQLLRRSIRRFSADPVAPEMIEAAVAEALTAPAPHHTR
CCCCCCEEEEECHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
PVRFVWVGDHATRIRLLDRMKDRWRDDLLADGLSADAVERRLARGQILYDAPELVIPFLV
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCEEEEEEE
PDGAHDYPDDTRTAAEHTMFTVAVGAAVQAFLVALAVREVGSCWVGSTIFAADVVRAELD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCC
LPAEWEPLGAVAVGHPAEPQGPRDPVLPEGLLVRR
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]