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The map label for this gene is nei [H]

Identifier: 119867332

GI number: 119867332

Start: 1375069

End: 1375842

Strand: Reverse

Name: nei [H]

Synonym: Mkms_1282

Alternate gene names: 119867332

Gene position: 1375842-1375069 (Counterclockwise)

Preceding gene: 119867334

Following gene: 119867331

Centisome position: 23.98

GC content: 69.12

Gene sequence:

>774_bases
ATGCCTGAAGGCGACACCGTCTACCGCACCGCCACCGCCTTGCGCGAAGGCCTGGTCGGCAAGACACTGACCCGCTGCGA
CGTCCGCGTGCCGCGCTACGCCACCGTCGATCTCACCGGCCACGTCGTCGACGAGGTGCTCAGTCGCGGCAAACACCTGT
TCATCCGCGTCGGCCCGGCCAGCATCCACTCCCACCTCAAGATGGAAGGCAGCTGGAAGGTCGTCCCGGCATCGCGGCCC
AGCCGCGCCGGGTACCGCATCCGCATCATCCTCGAGGCCGGCGAGGGTGAGCAGGCCGTGCAGGCCGCGGGCATCGACCT
CGGCGTGCTCGAGATCCTCGAGCGTGACCACGACATGGAGACCGTCGCCCATCTCGGACCCGACCTCCTCGGCGACGACT
GGGAGCCCCGAGTCGCGGCCCGGAACCTGAGCGCCGACCCGGATCGTCGGCTGTCCGAGGCCCTGCTGGACCAGCGCGTG
ATGGCCGGCGTCGGCAACGTCTACGCCAACGAACTGTGTTTCGTCGCCGGACACCTGCCGACCGCACCGGTGCGCGACCT
CAAAGATCCGTTACGGATGGTGCACCGCGCGCGGGATATGTTGTGGCTGAACAGGTCCCGCTGGAATCGCACCACCACCG
GCGACACCCGTCCCGGCCGCGACGTATGGGTTTACGGCCGGGCCGGCAAACCGTGCCGCCGCTGCGGCACACCGATCCGT
CGGGATGGCAACGGCGATCGTGTGTCGTACTGGTGCCCGCACTGTCAGCGCTGA

Upstream 100 bases:

>100_bases
CGTCGATGCGCATGGTCATGAGCCTGACGCAGCAACGCCATCACCGGTAGGTCCGAAAGTCCTTTCCTGGCCGGTTCGCG
GGCGATCGGTCTGATGAACC

Downstream 100 bases:

>100_bases
CGGCGTACGGGCGCCGACACGACGGTTTCTGATCGATTTCGGGCGGTTCCGCGCAACATCCGTAGTGTCCGCGCGGCAGT
AATTGCTTCAAAGCAAGAGT

Product: DNA-formamidopyrimidine glycosylase

Products: NA

Alternate protein names: Putative DNA-(apurinic or apyrimidinic site) lyase Rv3297/MT3396; Putative AP lyase Rv3297/MT3396 [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MPEGDTVYRTATALREGLVGKTLTRCDVRVPRYATVDLTGHVVDEVLSRGKHLFIRVGPASIHSHLKMEGSWKVVPASRP
SRAGYRIRIILEAGEGEQAVQAAGIDLGVLEILERDHDMETVAHLGPDLLGDDWEPRVAARNLSADPDRRLSEALLDQRV
MAGVGNVYANELCFVAGHLPTAPVRDLKDPLRMVHRARDMLWLNRSRWNRTTTGDTRPGRDVWVYGRAGKPCRRCGTPIR
RDGNGDRVSYWCPHCQR

Sequences:

>Translated_257_residues
MPEGDTVYRTATALREGLVGKTLTRCDVRVPRYATVDLTGHVVDEVLSRGKHLFIRVGPASIHSHLKMEGSWKVVPASRP
SRAGYRIRIILEAGEGEQAVQAAGIDLGVLEILERDHDMETVAHLGPDLLGDDWEPRVAARNLSADPDRRLSEALLDQRV
MAGVGNVYANELCFVAGHLPTAPVRDLKDPLRMVHRARDMLWLNRSRWNRTTTGDTRPGRDVWVYGRAGKPCRRCGTPIR
RDGNGDRVSYWCPHCQR
>Mature_256_residues
PEGDTVYRTATALREGLVGKTLTRCDVRVPRYATVDLTGHVVDEVLSRGKHLFIRVGPASIHSHLKMEGSWKVVPASRPS
RAGYRIRIILEAGEGEQAVQAAGIDLGVLEILERDHDMETVAHLGPDLLGDDWEPRVAARNLSADPDRRLSEALLDQRVM
AGVGNVYANELCFVAGHLPTAPVRDLKDPLRMVHRARDMLWLNRSRWNRTTTGDTRPGRDVWVYGRAGKPCRRCGTPIRR
DGNGDRVSYWCPHCQR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA b

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger [H]

Homologues:

Organism=Homo sapiens, GI157388969, Length=220, Percent_Identity=24.5454545454545, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1790066, Length=272, Percent_Identity=27.2058823529412, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786932, Length=268, Percent_Identity=27.6119402985075, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR012319
- InterPro:   IPR010979
- InterPro:   IPR000214 [H]

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 28755; Mature: 28624

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS01242 ZF_FPG_1 ; PS51066 ZF_FPG_2 ; PS51068 FPG_CAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGDTVYRTATALREGLVGKTLTRCDVRVPRYATVDLTGHVVDEVLSRGKHLFIRVGPA
CCCCCHHHHHHHHHHHCHHHCHHHHHCCCCCCEEEEECHHHHHHHHHHCCCEEEEEECHH
SIHSHLKMEGSWKVVPASRPSRAGYRIRIILEAGEGEQAVQAAGIDLGVLEILERDHDME
HHHHHEEECCCEEEEECCCCCCCCEEEEEEEECCCCHHHHHHCCCCHHHHHHHHCCCCHH
TVAHLGPDLLGDDWEPRVAARNLSADPDRRLSEALLDQRVMAGVGNVYANELCFVAGHLP
HHHHCCCHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCC
TAPVRDLKDPLRMVHRARDMLWLNRSRWNRTTTGDTRPGRDVWVYGRAGKPCRRCGTPIR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCC
RDGNGDRVSYWCPHCQR
CCCCCCEEEEECCCCCC
>Mature Secondary Structure 
PEGDTVYRTATALREGLVGKTLTRCDVRVPRYATVDLTGHVVDEVLSRGKHLFIRVGPA
CCCCHHHHHHHHHHHCHHHCHHHHHCCCCCCEEEEECHHHHHHHHHHCCCEEEEEECHH
SIHSHLKMEGSWKVVPASRPSRAGYRIRIILEAGEGEQAVQAAGIDLGVLEILERDHDME
HHHHHEEECCCEEEEECCCCCCCCEEEEEEEECCCCHHHHHHCCCCHHHHHHHHCCCCHH
TVAHLGPDLLGDDWEPRVAARNLSADPDRRLSEALLDQRVMAGVGNVYANELCFVAGHLP
HHHHCCCHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCC
TAPVRDLKDPLRMVHRARDMLWLNRSRWNRTTTGDTRPGRDVWVYGRAGKPCRRCGTPIR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCC
RDGNGDRVSYWCPHCQR
CCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]