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The map label for this gene is echA12 [H]

Identifier: 119867321

GI number: 119867321

Start: 1361594

End: 1362373

Strand: Reverse

Name: echA12 [H]

Synonym: Mkms_1271

Alternate gene names: 119867321

Gene position: 1362373-1361594 (Counterclockwise)

Preceding gene: 119867323

Following gene: 119867315

Centisome position: 23.75

GC content: 68.72

Gene sequence:

>780_bases
ATGACCGTCCTGGAGCTGACGCACCCCCGGCCCGACATCGCCGTCATCACCCTCAACCGTCCCGAGAAGCTCAACGCCCT
GTCCTACGAACTCGTCGAGGCCTTCCACACGACGCTCGGCGAGATCGCCGCGGACAACGAGTGTCGTGTGGTGGTGCTGA
CCGGGGCCGGGCGCGGATTCTGTTCCGGTCTGGACCTCACCGCTCCGAATCCGCCGCAGGCCGGCGGCGGTACCGAGTTC
CCGCGCTCGGGTATGCGCTGGCAGGAACGCATCGCCGAGCTGACCGTCCGGCTGCACCGGCTGCGCCAACCGGTAATCGC
CGCGGTGAACGGTCCGGCGTACGGCGGCGGGTTCGCGATCGCGGCGGCCGCCGACATCCGCCTGGCATCCGAAACCGCCC
GGTTCTGCACACAATTCATCAAACTCGGCCTCGGCGGCTGCGATATCGGGGTGAGCTACACGCTGCCGCGCATCATCGGC
GCGGGCCACGCGTTCGACCTGATCCTCACCGCCCGCCAGGTCGACGCCGATGAGGCGCTGCGCCTGGGTCTGGTGTCGCG
GGTGTCGGCGAACGTGCTCGACGACGCGCTGGCGATCGCCGAAACCCTCTGCGGCTACGGAAAATTCGGCCTCGAGTCGA
CCAAACAGGTGCTGTGGGCGAACCTCGCGGCGGGCAGCCTCGAACAGGCGCTGCACCTGGAGAACCGCAGCCAGATCCTG
GCGTCGACCACCGGTGAGGTGCGCGAAGCTAGCGAGGCGTTCCGCCACCGTCCACGATGA

Upstream 100 bases:

>100_bases
GCCGTCACCAACGTCCGTGGTCAGTACAGCTAGCGACGCGGGCCGGCACGTCTCCGCCGAACGCCTCGAAACCGGGCCCC
GAACACCTAGCGTGTCGACC

Downstream 100 bases:

>100_bases
TCGCCTGACCGGTCAGGTAGCTGCCGGCGTCGGAGACCAACAACAGTGCGGCGCCGACCATTTCGTCCGGTGATGCCAAC
CGGTTCATCAGCGTGGCCCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTVLELTHPRPDIAVITLNRPEKLNALSYELVEAFHTTLGEIAADNECRVVVLTGAGRGFCSGLDLTAPNPPQAGGGTEF
PRSGMRWQERIAELTVRLHRLRQPVIAAVNGPAYGGGFAIAAAADIRLASETARFCTQFIKLGLGGCDIGVSYTLPRIIG
AGHAFDLILTARQVDADEALRLGLVSRVSANVLDDALAIAETLCGYGKFGLESTKQVLWANLAAGSLEQALHLENRSQIL
ASTTGEVREASEAFRHRPR

Sequences:

>Translated_259_residues
MTVLELTHPRPDIAVITLNRPEKLNALSYELVEAFHTTLGEIAADNECRVVVLTGAGRGFCSGLDLTAPNPPQAGGGTEF
PRSGMRWQERIAELTVRLHRLRQPVIAAVNGPAYGGGFAIAAAADIRLASETARFCTQFIKLGLGGCDIGVSYTLPRIIG
AGHAFDLILTARQVDADEALRLGLVSRVSANVLDDALAIAETLCGYGKFGLESTKQVLWANLAAGSLEQALHLENRSQIL
ASTTGEVREASEAFRHRPR
>Mature_258_residues
TVLELTHPRPDIAVITLNRPEKLNALSYELVEAFHTTLGEIAADNECRVVVLTGAGRGFCSGLDLTAPNPPQAGGGTEFP
RSGMRWQERIAELTVRLHRLRQPVIAAVNGPAYGGGFAIAAAADIRLASETARFCTQFIKLGLGGCDIGVSYTLPRIIGA
GHAFDLILTARQVDADEALRLGLVSRVSANVLDDALAIAETLCGYGKFGLESTKQVLWANLAAGSLEQALHLENRSQILA
STTGEVREASEAFRHRPR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=225, Percent_Identity=36, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=28.1746031746032, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI116235487, Length=166, Percent_Identity=33.1325301204819, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI4757966, Length=229, Percent_Identity=27.5109170305677, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI51538581, Length=229, Percent_Identity=27.5109170305677, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI25453481, Length=229, Percent_Identity=27.5109170305677, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI51538573, Length=229, Percent_Identity=27.5109170305677, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4757968, Length=229, Percent_Identity=27.5109170305677, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI49355787, Length=229, Percent_Identity=27.5109170305677, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI20127408, Length=180, Percent_Identity=31.1111111111111, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI45643119, Length=183, Percent_Identity=31.6939890710383, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI260275230, Length=183, Percent_Identity=31.6939890710383, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI260274832, Length=183, Percent_Identity=31.6939890710383, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI221136756, Length=221, Percent_Identity=28.9592760180996, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI221136753, Length=221, Percent_Identity=28.9592760180996, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI221307494, Length=221, Percent_Identity=28.9592760180996, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI68989263, Length=190, Percent_Identity=29.4736842105263, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI31542718, Length=225, Percent_Identity=27.1111111111111, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787660, Length=256, Percent_Identity=35.546875, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI221142681, Length=209, Percent_Identity=36.3636363636364, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787659, Length=208, Percent_Identity=32.2115384615385, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788597, Length=177, Percent_Identity=32.2033898305085, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788682, Length=181, Percent_Identity=32.0441988950276, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790281, Length=243, Percent_Identity=26.7489711934156, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082183, Length=178, Percent_Identity=26.4044943820225, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17534483, Length=250, Percent_Identity=32.8, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17536985, Length=226, Percent_Identity=29.2035398230088, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17540714, Length=238, Percent_Identity=33.6134453781513, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=265, Percent_Identity=25.6603773584906, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=265, Percent_Identity=30.9433962264151, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=266, Percent_Identity=29.3233082706767, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=175, Percent_Identity=32, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=220, Percent_Identity=30, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=200, Percent_Identity=27.5, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=193, Percent_Identity=28.4974093264249, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=188, Percent_Identity=30.8510638297872, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920382, Length=243, Percent_Identity=29.6296296296296, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24653139, Length=243, Percent_Identity=35.8024691358025, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI20129971, Length=259, Percent_Identity=30.1158301158301, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653477, Length=259, Percent_Identity=30.1158301158301, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24654903, Length=183, Percent_Identity=31.1475409836066, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357171, Length=232, Percent_Identity=27.5862068965517, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24650670, Length=252, Percent_Identity=25.7936507936508, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27712; Mature: 27581

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLELTHPRPDIAVITLNRPEKLNALSYELVEAFHTTLGEIAADNECRVVVLTGAGRGF
CEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
CSGLDLTAPNPPQAGGGTEFPRSGMRWQERIAELTVRLHRLRQPVIAAVNGPAYGGGFAI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHEEEECCCCCCCCEEE
AAAADIRLASETARFCTQFIKLGLGGCDIGVSYTLPRIIGAGHAFDLILTARQVDADEAL
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHH
RLGLVSRVSANVLDDALAIAETLCGYGKFGLESTKQVLWANLAAGSLEQALHLENRSQIL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
ASTTGEVREASEAFRHRPR
HHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVLELTHPRPDIAVITLNRPEKLNALSYELVEAFHTTLGEIAADNECRVVVLTGAGRGF
EEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
CSGLDLTAPNPPQAGGGTEFPRSGMRWQERIAELTVRLHRLRQPVIAAVNGPAYGGGFAI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHEEEECCCCCCCCEEE
AAAADIRLASETARFCTQFIKLGLGGCDIGVSYTLPRIIGAGHAFDLILTARQVDADEAL
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHH
RLGLVSRVSANVLDDALAIAETLCGYGKFGLESTKQVLWANLAAGSLEQALHLENRSQIL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
ASTTGEVREASEAFRHRPR
HHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]