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The map label for this gene is trmB

Identifier: 119866238

GI number: 119866238

Start: 198123

End: 198950

Strand: Reverse

Name: trmB

Synonym: Mkms_0182

Alternate gene names: 119866238

Gene position: 198950-198123 (Counterclockwise)

Preceding gene: 119866244

Following gene: 119866237

Centisome position: 3.47

GC content: 72.95

Gene sequence:

>828_bases
ATGAGACACCATGGACGGATGCACGCGCGCGAGTCTGATGTCGCCGGCGGGCCGGCGCGTGACTCAGCCGGCGGGCCGGC
GCGTGACTCAGCCGGCGGGCCGGCGCGTGACTCAGCCGGGGACGACTCCCCCTCAGCGCACCGTCACCGGCGGGTGACGA
GCTTCCGGTCGCGCCGTTCGGCGCTGTCCGGAGGACAGCAGGACACCTGGGAGCGGCTGTGGCCGGAGCTCGGCACCGAG
GCGCGTGACGACGACGGCCGACCGGCGGCGCTGATCGACACCGCGGCGTGGTTCGGCCGGACCGCACCGCTGGTCCTGGA
GATCGGGTCGGGCACCGGCACCTCGACGCTGGCGATGGCCCAGGACGAACCGGACATCGACGTCATCGCGGTGGAGGTTT
ACCGGCGCGGCCTCGCCCAGCTGCTCACCGGCATCGACCGGGCCGGGGTGCGCAACATCAGGATGATCCGCGGTGACGGC
GTCGACGTGCTCGAGTACATGGTGGCGAGCGGCTCGTTGACCGGCGTGCGGGTGTTCTTCCCCGACCCGTGGCCCAAGGC
CCGCCACCACAAACGACGGCTGCTGCAGCCGTCGACCGTGGCGCTGATCGCCGACCGGCTGCGTCCCGGCGGCATCCTGC
ACGCGGCCACCGACCACGCCGGCTACGCCGAGCAGATCGCGGCGGTGGGCGACGCCGAACCGCTGCTGCGCCGGGTCGAT
CTCACCGATGACCTGCCGATCTCGGTGCGCCGGCCCGTCACGAAGTACGAGCGCAAGGCCCTGGCCGGCCCCGACGTCGC
CGAGCTGCTCTGGGAGAAGGTGCCGTGA

Upstream 100 bases:

>100_bases
TGGCATCTGTCGGGGCGCCTCACGTACGGATGTTTGGCGTACCAGCCGAAGGCAACTGTTGGGTATCAATCTGTACCACG
ATGGGGGTCCCCCGGCACTG

Downstream 100 bases:

>100_bases
GCGTCACCGAAGACCTTCCGCAGGAGATCCCGCAGGTCACGCCGCCCCCGCCGCCCGCCGGTCCGCCCGCCGGAACGCAG
CGGGTGCTGCTGGTGTGGGA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRHHGRMHARESDVAGGPARDSAGGPARDSAGGPARDSAGDDSPSAHRHRRVTSFRSRRSALSGGQQDTWERLWPELGTE
ARDDDGRPAALIDTAAWFGRTAPLVLEIGSGTGTSTLAMAQDEPDIDVIAVEVYRRGLAQLLTGIDRAGVRNIRMIRGDG
VDVLEYMVASGSLTGVRVFFPDPWPKARHHKRRLLQPSTVALIADRLRPGGILHAATDHAGYAEQIAAVGDAEPLLRRVD
LTDDLPISVRRPVTKYERKALAGPDVAELLWEKVP

Sequences:

>Translated_275_residues
MRHHGRMHARESDVAGGPARDSAGGPARDSAGGPARDSAGDDSPSAHRHRRVTSFRSRRSALSGGQQDTWERLWPELGTE
ARDDDGRPAALIDTAAWFGRTAPLVLEIGSGTGTSTLAMAQDEPDIDVIAVEVYRRGLAQLLTGIDRAGVRNIRMIRGDG
VDVLEYMVASGSLTGVRVFFPDPWPKARHHKRRLLQPSTVALIADRLRPGGILHAATDHAGYAEQIAAVGDAEPLLRRVD
LTDDLPISVRRPVTKYERKALAGPDVAELLWEKVP
>Mature_275_residues
MRHHGRMHARESDVAGGPARDSAGGPARDSAGGPARDSAGDDSPSAHRHRRVTSFRSRRSALSGGQQDTWERLWPELGTE
ARDDDGRPAALIDTAAWFGRTAPLVLEIGSGTGTSTLAMAQDEPDIDVIAVEVYRRGLAQLLTGIDRAGVRNIRMIRGDG
VDVLEYMVASGSLTGVRVFFPDPWPKARHHKRRLLQPSTVALIADRLRPGGILHAATDHAGYAEQIAAVGDAEPLLRRVD
LTDDLPISVRRPVTKYERKALAGPDVAELLWEKVP

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=227, Percent_Identity=38.7665198237885, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_MYCSK (A1U992)

Other databases:

- EMBL:   CP000518
- RefSeq:   YP_936190.1
- ProteinModelPortal:   A1U992
- STRING:   A1U992
- EnsemblBacteria:   EBMYCT00000056904
- GeneID:   4615411
- GenomeReviews:   CP000518_GR
- KEGG:   mkm:Mkms_0182
- eggNOG:   COG0220
- GeneTree:   EBGT00050000017066
- HOGENOM:   HBG322782
- OMA:   NIRLIRG
- ProtClustDB:   PRK00121
- BioCyc:   MSP189918:MKMS_0182-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 29801; Mature: 29801

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: BINDING 107-107 BINDING 132-132 BINDING 159-159 BINDING 182-182 BINDING 186-186 BINDING 218-218

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHHGRMHARESDVAGGPARDSAGGPARDSAGGPARDSAGDDSPSAHRHRRVTSFRSRRS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ALSGGQQDTWERLWPELGTEARDDDGRPAALIDTAAWFGRTAPLVLEIGSGTGTSTLAMA
HHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEC
QDEPDIDVIAVEVYRRGLAQLLTGIDRAGVRNIRMIRGDGVDVLEYMVASGSLTGVRVFF
CCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEEC
PDPWPKARHHKRRLLQPSTVALIADRLRPGGILHAATDHAGYAEQIAAVGDAEPLLRRVD
CCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHCCCHHHHHHHHC
LTDDLPISVRRPVTKYERKALAGPDVAELLWEKVP
CCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCC
>Mature Secondary Structure
MRHHGRMHARESDVAGGPARDSAGGPARDSAGGPARDSAGDDSPSAHRHRRVTSFRSRRS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ALSGGQQDTWERLWPELGTEARDDDGRPAALIDTAAWFGRTAPLVLEIGSGTGTSTLAMA
HHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEC
QDEPDIDVIAVEVYRRGLAQLLTGIDRAGVRNIRMIRGDGVDVLEYMVASGSLTGVRVFF
CCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEEC
PDPWPKARHHKRRLLQPSTVALIADRLRPGGILHAATDHAGYAEQIAAVGDAEPLLRRVD
CCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHCCCHHHHHHHHC
LTDDLPISVRRPVTKYERKALAGPDVAELLWEKVP
CCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA