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The map label for this gene is dnaB [H]

Identifier: 119855254

GI number: 119855254

Start: 73081

End: 74475

Strand: Reverse

Name: dnaB [H]

Synonym: Mkms_5868

Alternate gene names: 119855254

Gene position: 74475-73081 (Counterclockwise)

Preceding gene: 119855257

Following gene: 119855249

Centisome position: 34.36

GC content: 65.95

Gene sequence:

>1395_bases
ATGACCGATCTCGACGACGCCCCCGTGCGGGCGCCCCGCCGGCAGCAGGTGGAGTCCTACACCGATCTCGACGACCGACG
GCAACCGCCGCAAGACCAGGCCGCCGAGCAGTCGGTGCTGGGCGGCATGCTGCTGAGCAAGGACGCGATCGCCGACGTCC
TCGAGAAGCTGCGGCCCAACGACTTCTACCGACCCAACCACCAGCAGATCTACAACGCGATCCTGGACCTGTTCGGTCAG
GGTGAACCCGCTGACGCCGTGACCGTGGCGGCCGAGCTGGACCGCCGCGGGCTGCTGCGCCGCATCGGCGGCGCGCCGTA
TCTGCACACGCTGATCGCCACCGTGCCGACGGCAGCCAACGCCGGCTACTACGCGGGCATCGTGGCCGAGAAGGCGGTGC
TGCGGCGACTGGTGGAGGCCGGCACCCGGGTCGTGCAGTACGGCTACGCCGGCGCCGACGGCGCTGACGTCGCTGAGGTC
GTCGACCGCGCGCAAGCCGAGATCTACGACGTCACAGAGGACCGCGGCAGCAATGACATGGTGGCGCTGGAGGACCTGCT
GCAGCCCACCATGGACGAGATCGACGCCATCCAATCCGGCGGCGGTCAAGCCCAAGGGGTGCCTACCGGATTCGCCGAAC
TCGACGAGGTGACCACCGGCCTTCACGGTGGACAAATGATCATCGTCGCGGCTCGACCTGGCCACGGGAAGTCCACGCTG
GCATTGGATTTCATGCGTTCGTGCTCGATCAAACACGGTATGGCCAGCGTCATCTTCAGTCTGGAGATGAGTAAGTCAGA
GATCGTGATGCGTCTGTTGTCGGCCGAAGCGCGCATCAAGCTGGCCGACATGCGTTCGGGCCGGATGAGTGACGACGACT
GGACGAAGATGGCGCGGCGAATGGGCGAGATCACCGAGGCCCCGCTCTACATCGACGACTCACCCAACCTGACGATGATG
GAGATCCGCGCCAAAGCCCGCCGGCTCAAGCAACGCACCGATCTGCGGCTGATCGTCGTTGACTACCTCCAGCTCATGAC
GTCCGGGAAGCGGGTGGAGTCCCGACAGGCCGAGGTGGCCGAGTTCTCGCGCAGCCTCAAGCTGATGGCCAAGGAACTCG
ATGTGCCGGTGGTGGCGATCAGCCAGCTCAACCGCGGCCCCGAGCAGCGCACCGACAAGGTGCCCCAGCTGTCTGACCTT
CGCGAAACCGGCCAATTGGAGCAGGACGGGGACATGGTGATCCTGATCAACCGGCCCGACGCCTACGACCGTGACAGCCC
GCGCGCCGGCGAAGCGGACCTCATCCTGGCCAAACATCGCAACGGGCCGACGAAGACGTGCACGGTGGCCAGTACTCTGC
ATCTAGCGAGCTTCCGCGACCTCGCACGCTACTGA

Upstream 100 bases:

>100_bases
TCAAGGCGATGGCGACGGTGTGCGAGCAGTGCTGGCCCCACATCGCCGCGGACCTGGCCGCTGGTGAGCAATACCCGGAC
ATCGAGGAGGCGATCCCGGC

Downstream 100 bases:

>100_bases
ACAAGCGAATTATGTTCTGCGAGAACGCAACTCGACAGCGATGTCGACAGCGTACTGGCGTTGCGCGGCGGTGTACAAGA
TGCCGGCGGCCGTGTCGGTG

Product: replicative DNA helicase

Products: NA

Alternate protein names: Mbo dnaB intein [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTDLDDAPVRAPRRQQVESYTDLDDRRQPPQDQAAEQSVLGGMLLSKDAIADVLEKLRPNDFYRPNHQQIYNAILDLFGQ
GEPADAVTVAAELDRRGLLRRIGGAPYLHTLIATVPTAANAGYYAGIVAEKAVLRRLVEAGTRVVQYGYAGADGADVAEV
VDRAQAEIYDVTEDRGSNDMVALEDLLQPTMDEIDAIQSGGGQAQGVPTGFAELDEVTTGLHGGQMIIVAARPGHGKSTL
ALDFMRSCSIKHGMASVIFSLEMSKSEIVMRLLSAEARIKLADMRSGRMSDDDWTKMARRMGEITEAPLYIDDSPNLTMM
EIRAKARRLKQRTDLRLIVVDYLQLMTSGKRVESRQAEVAEFSRSLKLMAKELDVPVVAISQLNRGPEQRTDKVPQLSDL
RETGQLEQDGDMVILINRPDAYDRDSPRAGEADLILAKHRNGPTKTCTVASTLHLASFRDLARY

Sequences:

>Translated_464_residues
MTDLDDAPVRAPRRQQVESYTDLDDRRQPPQDQAAEQSVLGGMLLSKDAIADVLEKLRPNDFYRPNHQQIYNAILDLFGQ
GEPADAVTVAAELDRRGLLRRIGGAPYLHTLIATVPTAANAGYYAGIVAEKAVLRRLVEAGTRVVQYGYAGADGADVAEV
VDRAQAEIYDVTEDRGSNDMVALEDLLQPTMDEIDAIQSGGGQAQGVPTGFAELDEVTTGLHGGQMIIVAARPGHGKSTL
ALDFMRSCSIKHGMASVIFSLEMSKSEIVMRLLSAEARIKLADMRSGRMSDDDWTKMARRMGEITEAPLYIDDSPNLTMM
EIRAKARRLKQRTDLRLIVVDYLQLMTSGKRVESRQAEVAEFSRSLKLMAKELDVPVVAISQLNRGPEQRTDKVPQLSDL
RETGQLEQDGDMVILINRPDAYDRDSPRAGEADLILAKHRNGPTKTCTVASTLHLASFRDLARY
>Mature_463_residues
TDLDDAPVRAPRRQQVESYTDLDDRRQPPQDQAAEQSVLGGMLLSKDAIADVLEKLRPNDFYRPNHQQIYNAILDLFGQG
EPADAVTVAAELDRRGLLRRIGGAPYLHTLIATVPTAANAGYYAGIVAEKAVLRRLVEAGTRVVQYGYAGADGADVAEVV
DRAQAEIYDVTEDRGSNDMVALEDLLQPTMDEIDAIQSGGGQAQGVPTGFAELDEVTTGLHGGQMIIVAARPGHGKSTLA
LDFMRSCSIKHGMASVIFSLEMSKSEIVMRLLSAEARIKLADMRSGRMSDDDWTKMARRMGEITEAPLYIDDSPNLTMME
IRAKARRLKQRTDLRLIVVDYLQLMTSGKRVESRQAEVAEFSRSLKLMAKELDVPVVAISQLNRGPEQRTDKVPQLSDLR
ETGQLEQDGDMVILINRPDAYDRDSPRAGEADLILAKHRNGPTKTCTVASTLHLASFRDLARY

Specific function: The intein is an endonuclease (Potential) [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=439, Percent_Identity=38.7243735763098, Blast_Score=306, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51108; Mature: 50977

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLDDAPVRAPRRQQVESYTDLDDRRQPPQDQAAEQSVLGGMLLSKDAIADVLEKLRPN
CCCCCCCCCCCCHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DFYRPNHQQIYNAILDLFGQGEPADAVTVAAELDRRGLLRRIGGAPYLHTLIATVPTAAN
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
AGYYAGIVAEKAVLRRLVEAGTRVVQYGYAGADGADVAEVVDRAQAEIYDVTEDRGSNDM
CCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCH
VALEDLLQPTMDEIDAIQSGGGQAQGVPTGFAELDEVTTGLHGGQMIIVAARPGHGKSTL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHH
ALDFMRSCSIKHGMASVIFSLEMSKSEIVMRLLSAEARIKLADMRSGRMSDDDWTKMARR
HHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHH
MGEITEAPLYIDDSPNLTMMEIRAKARRLKQRTDLRLIVVDYLQLMTSGKRVESRQAEVA
HHHHHCCCEEECCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHH
EFSRSLKLMAKELDVPVVAISQLNRGPEQRTDKVPQLSDLRETGQLEQDGDMVILINRPD
HHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCCCCHHHHHHHCCCCCCCCEEEEEECCC
AYDRDSPRAGEADLILAKHRNGPTKTCTVASTLHLASFRDLARY
CCCCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDLDDAPVRAPRRQQVESYTDLDDRRQPPQDQAAEQSVLGGMLLSKDAIADVLEKLRPN
CCCCCCCCCCCHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DFYRPNHQQIYNAILDLFGQGEPADAVTVAAELDRRGLLRRIGGAPYLHTLIATVPTAAN
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
AGYYAGIVAEKAVLRRLVEAGTRVVQYGYAGADGADVAEVVDRAQAEIYDVTEDRGSNDM
CCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCH
VALEDLLQPTMDEIDAIQSGGGQAQGVPTGFAELDEVTTGLHGGQMIIVAARPGHGKSTL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHH
ALDFMRSCSIKHGMASVIFSLEMSKSEIVMRLLSAEARIKLADMRSGRMSDDDWTKMARR
HHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHH
MGEITEAPLYIDDSPNLTMMEIRAKARRLKQRTDLRLIVVDYLQLMTSGKRVESRQAEVA
HHHHHCCCEEECCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHH
EFSRSLKLMAKELDVPVVAISQLNRGPEQRTDKVPQLSDLRETGQLEQDGDMVILINRPD
HHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCCCCHHHHHHHCCCCCCCCEEEEEECCC
AYDRDSPRAGEADLILAKHRNGPTKTCTVASTLHLASFRDLARY
CCCCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]