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The map label for this gene is rph

Identifier: 119900252

GI number: 119900252

Start: 4344315

End: 4345034

Strand: Direct

Name: rph

Synonym: azo3963

Alternate gene names: 119900252

Gene position: 4344315-4345034 (Clockwise)

Preceding gene: 119900251

Following gene: 119900253

Centisome position: 99.28

GC content: 70.28

Gene sequence:

>720_bases
ATGCGACCCAGCCAGCGCCAAGCCAACGAACTCCGCCCCGTCCGCCTCACCCGCCGCTACACGCGCCACGCCGAAGGATC
GGTGCTGGTCGAGTTCGGCGACACCCGGGTGCTGTGCACCGCCAGCGTGGAGGAAGCGGTGCCGCCTTTCCTCAAGGGCA
AGGGCCGCGGCTGGGTCACCGCCGAATACGGCATGCTGCCGCGCGCCACCCACACCCGCAGCGCGCGCGAAGCCGCCAAG
GGCAAGCAGACCGGCCGCACCCAGGAAATCCAGCGCCTGATCGGCCGCAGCCTGCGCGCGGTGGTGGATCTGGCCGCGCT
CGGCGAACGCCAGGTGGTGATCGATTGCGACGTGCTGCAGGCCGACGGCGGCACCCGCACCGCGTCGATCACCGGCGCGT
ACGTCGCGTTGCACGATGCGCTCGAAGGCCTGGTGGCCGCCGGCAAGCTCGCCGCCAACCCGATGACCGACTTCGTTGCC
GCGATCTCGGTCGGGATCGTCGACGGGGTGCCGGTGCTCGACCTCGACTACACTGAAGATTCGGGCTGCGACACCGACAT
GAACGTGGTGATGACCGGCAGCGGCGGCATCATCGAAGTGCAGGGCACCGCCGAGGGCACGCCCTTCTCGCGCACCGAAC
TCAACGCCCTGATCGACCTCGCCGAAGCCGGCATCGGCCGCCTCGTGGAACTGCAGAAGGCGGCGCTGGCGGGAGCCTGA

Upstream 100 bases:

>100_bases
CTCGATCTCTCGGACGACGAAATCGAGAACGCCATCGCCGAGATCAACGCCGCGATCCACAAATTCTCCAAACCCTGAGC
CTCCTCAGCAGACCCGACCC

Downstream 100 bases:

>100_bases
CGCATGAGCAAACGACTCGTCCTTGCCAGCAACAACGCCAAGAAAGCCGCCGAGATGCAGGCCCTGCTCGCGCCGCTCGG
CATCGAGGTGGTGGCGCAGG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRPSQRQANELRPVRLTRRYTRHAEGSVLVEFGDTRVLCTASVEEAVPPFLKGKGRGWVTAEYGMLPRATHTRSAREAAK
GKQTGRTQEIQRLIGRSLRAVVDLAALGERQVVIDCDVLQADGGTRTASITGAYVALHDALEGLVAAGKLAANPMTDFVA
AISVGIVDGVPVLDLDYTEDSGCDTDMNVVMTGSGGIIEVQGTAEGTPFSRTELNALIDLAEAGIGRLVELQKAALAGA

Sequences:

>Translated_239_residues
MRPSQRQANELRPVRLTRRYTRHAEGSVLVEFGDTRVLCTASVEEAVPPFLKGKGRGWVTAEYGMLPRATHTRSAREAAK
GKQTGRTQEIQRLIGRSLRAVVDLAALGERQVVIDCDVLQADGGTRTASITGAYVALHDALEGLVAAGKLAANPMTDFVA
AISVGIVDGVPVLDLDYTEDSGCDTDMNVVMTGSGGIIEVQGTAEGTPFSRTELNALIDLAEAGIGRLVELQKAALAGA
>Mature_239_residues
MRPSQRQANELRPVRLTRRYTRHAEGSVLVEFGDTRVLCTASVEEAVPPFLKGKGRGWVTAEYGMLPRATHTRSAREAAK
GKQTGRTQEIQRLIGRSLRAVVDLAALGERQVVIDCDVLQADGGTRTASITGAYVALHDALEGLVAAGKLAANPMTDFVA
AISVGIVDGVPVLDLDYTEDSGCDTDMNVVMTGSGGIIEVQGTAEGTPFSRTELNALIDLAEAGIGRLVELQKAALAGA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=73.4597156398104, Blast_Score=317, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI71981632, Length=183, Percent_Identity=27.8688524590164, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_AZOSB (A1KCM3)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_935465.1
- ProteinModelPortal:   A1KCM3
- SMR:   A1KCM3
- STRING:   A1KCM3
- GeneID:   4608213
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3963
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   A1KCM3
- ProtClustDB:   PRK00173
- BioCyc:   ASP62928:AZO3963-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25272; Mature: 25272

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSQRQANELRPVRLTRRYTRHAEGSVLVEFGDTRVLCTASVEEAVPPFLKGKGRGWVT
CCCCCCCHHCCCHHHHHHHHHHCCCCCEEEEECCCEEEEECCHHHHCCHHHCCCCCEEEE
AEYGMLPRATHTRSAREAAKGKQTGRTQEIQRLIGRSLRAVVDLAALGERQVVIDCDVLQ
ECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTASITGAYVALHDALEGLVAAGKLAANPMTDFVAAISVGIVDGVPVLDLDYTED
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
SGCDTDMNVVMTGSGGIIEVQGTAEGTPFSRTELNALIDLAEAGIGRLVELQKAALAGA
CCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPSQRQANELRPVRLTRRYTRHAEGSVLVEFGDTRVLCTASVEEAVPPFLKGKGRGWVT
CCCCCCCHHCCCHHHHHHHHHHCCCCCEEEEECCCEEEEECCHHHHCCHHHCCCCCEEEE
AEYGMLPRATHTRSAREAAKGKQTGRTQEIQRLIGRSLRAVVDLAALGERQVVIDCDVLQ
ECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTASITGAYVALHDALEGLVAAGKLAANPMTDFVAAISVGIVDGVPVLDLDYTED
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
SGCDTDMNVVMTGSGGIIEVQGTAEGTPFSRTELNALIDLAEAGIGRLVELQKAALAGA
CCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA