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The map label for this gene is phoA [H]

Identifier: 119900144

GI number: 119900144

Start: 4224451

End: 4226037

Strand: Direct

Name: phoA [H]

Synonym: azo3855

Alternate gene names: 119900144

Gene position: 4224451-4226037 (Clockwise)

Preceding gene: 119900143

Following gene: 119900145

Centisome position: 96.54

GC content: 68.3

Gene sequence:

>1587_bases
ATGCAACCCAAGGCCCTCGCCATCAGCATCGCCCTCGCCCTGTGCGCCGCACAGGCGCACGCCGCAGGCAACGCGGTTCC
CACCAGCGCCGAAGAATGGTTCGCCGCCGGCCGCGCCGCGGTGGAAACCTCCAAGCACGTGGTGCCCAACCGCGCCCGCG
CCAAGAACGTCATCCTCTTCGTCGGCGACGGCATGGGCGTCTCCACCGTCACCGCCGCGCGCATCCTGGAAGGCCAGATG
CGCAACGCCGACGGCGAGTTCAACCGCCTCAGCTTCGAAAAGCTGGACCACATGGGCACCTCGGTGACCGCGTCCGCCAA
CCAGCAGACCTCCGACTCCGCCCCGACCGCCACCGCGATGGTCACCGGCATCAAGACCAACGACGGCGCGATCTCGGTGG
ACCAGACCATCGAGCGCAACGAGCCGAGCGCCACTGTGACCCGCGCCAAGAGCGTCAAGACCATCCTCGAGCAGGCCGAG
GAACGCGGCATGTCCACCGGCATCGTGACCACCGCCCGCCTCACCCATGCCACGCCGGCGGTGAACTACGCCCACATCGG
CAACCGCGACTGGGAAGCCGACAGCAACCTGCCGGCCGGCGCCACCGTGGCCGACATCGCCCGCCAGCTGCTCGAATTCC
CCTATGGCGACGGCCTGGAAGTGGCGCTCGGCGGCGGCCGCAGCTACTTCATGCCGAACACCGCGAGCGACCCGGAATAC
CCCAGCCAGAAGGGCCGCCGCAAGGACGGCCGCGACCTGACCAAGGAATGGACGACGAAGTACAAGCAATCCGCCTACGT
CTGGGACAAGGCCGCGTTCGACGCGGTGAACCCGCAGCAGACCAAGCACCTGCTCGGCCTCTTCGAGCGTTCGCACATGC
GTTATGAGGCCGACCGCAAGGACGACGTCGCCGGCGAACCCTCGCTCGCCGAGATGACCGAGAAGGCGATCAAGATGCTG
GGCCAGAACAAGAAGGGCTTCTACCTGATGGTGGAAGCCGGCCGCATCGACCACGCCCACCACGCCGGCAACGCCTACCG
CGCGCTGACCGACACGGTGGCGCTGTCCGAAGCGGTGGAAGTGGCCAAGCGCCTGACCGACGACCAGGACACCCTGATCG
TCGTCACCGCCGACCACAGCCACGTGTTCACCATCGCCGGCTACCCGTCGCGCGGCAACCCGATCCTGGGCAAGAGCGCG
ATCGACGGCGTCGCCTCCACCGACGCGCTCGGCCTGACCTACACCACCGTGTCCTACGCCAACGGCCCGGGCTGGACCGG
CGGCTTCCAGCGCAAGGAATACAACCCGGCCACCGAAGGCAGCGTCGCGGCGCCGTACAACGGCAGCGCGCTGCGTCCCA
ACCTGGCCGGTATCGACACCACCGCGCCCAACTACATGCAGGAAGCGACCGTGCCGATGGGCTCCGAGACCCACGCCGGC
GAGGACGTGGCGATCTACGCCAGCGGCCCCAACGCCTACCTGTTCCGTGGCCCGCAGGAACAGAACGTGATCTATCACGT
GATGGCCGATGCGCTGGGCCTCAACAAGGGCGGGCATGGGCACGGGCACGGTCGCGGCCGCGATTGA

Upstream 100 bases:

>100_bases
CCGGCAACAAAAGCATCATGGGGGCGTCATGCGCGACTGGCACGCTGGCGGTCTGCAGTACCGCTCATCCAGCGCCATCC
GATCAGGGAGAACAACGACC

Downstream 100 bases:

>100_bases
TCGCAGCCATCCGGTGCCGGCCTCCGGTCGGCACCCAATCGGGGAGGCGCACCGGGTGCGCCTCCTTTTCCCGTTTTCAA
CGCCCCTTGCCGCCCGCCCA

Product: putative alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MQPKALAISIALALCAAQAHAAGNAVPTSAEEWFAAGRAAVETSKHVVPNRARAKNVILFVGDGMGVSTVTAARILEGQM
RNADGEFNRLSFEKLDHMGTSVTASANQQTSDSAPTATAMVTGIKTNDGAISVDQTIERNEPSATVTRAKSVKTILEQAE
ERGMSTGIVTTARLTHATPAVNYAHIGNRDWEADSNLPAGATVADIARQLLEFPYGDGLEVALGGGRSYFMPNTASDPEY
PSQKGRRKDGRDLTKEWTTKYKQSAYVWDKAAFDAVNPQQTKHLLGLFERSHMRYEADRKDDVAGEPSLAEMTEKAIKML
GQNKKGFYLMVEAGRIDHAHHAGNAYRALTDTVALSEAVEVAKRLTDDQDTLIVVTADHSHVFTIAGYPSRGNPILGKSA
IDGVASTDALGLTYTTVSYANGPGWTGGFQRKEYNPATEGSVAAPYNGSALRPNLAGIDTTAPNYMQEATVPMGSETHAG
EDVAIYASGPNAYLFRGPQEQNVIYHVMADALGLNKGGHGHGHGRGRD

Sequences:

>Translated_528_residues
MQPKALAISIALALCAAQAHAAGNAVPTSAEEWFAAGRAAVETSKHVVPNRARAKNVILFVGDGMGVSTVTAARILEGQM
RNADGEFNRLSFEKLDHMGTSVTASANQQTSDSAPTATAMVTGIKTNDGAISVDQTIERNEPSATVTRAKSVKTILEQAE
ERGMSTGIVTTARLTHATPAVNYAHIGNRDWEADSNLPAGATVADIARQLLEFPYGDGLEVALGGGRSYFMPNTASDPEY
PSQKGRRKDGRDLTKEWTTKYKQSAYVWDKAAFDAVNPQQTKHLLGLFERSHMRYEADRKDDVAGEPSLAEMTEKAIKML
GQNKKGFYLMVEAGRIDHAHHAGNAYRALTDTVALSEAVEVAKRLTDDQDTLIVVTADHSHVFTIAGYPSRGNPILGKSA
IDGVASTDALGLTYTTVSYANGPGWTGGFQRKEYNPATEGSVAAPYNGSALRPNLAGIDTTAPNYMQEATVPMGSETHAG
EDVAIYASGPNAYLFRGPQEQNVIYHVMADALGLNKGGHGHGHGRGRD
>Mature_528_residues
MQPKALAISIALALCAAQAHAAGNAVPTSAEEWFAAGRAAVETSKHVVPNRARAKNVILFVGDGMGVSTVTAARILEGQM
RNADGEFNRLSFEKLDHMGTSVTASANQQTSDSAPTATAMVTGIKTNDGAISVDQTIERNEPSATVTRAKSVKTILEQAE
ERGMSTGIVTTARLTHATPAVNYAHIGNRDWEADSNLPAGATVADIARQLLEFPYGDGLEVALGGGRSYFMPNTASDPEY
PSQKGRRKDGRDLTKEWTTKYKQSAYVWDKAAFDAVNPQQTKHLLGLFERSHMRYEADRKDDVAGEPSLAEMTEKAIKML
GQNKKGFYLMVEAGRIDHAHHAGNAYRALTDTVALSEAVEVAKRLTDDQDTLIVVTADHSHVFTIAGYPSRGNPILGKSA
IDGVASTDALGLTYTTVSYANGPGWTGGFQRKEYNPATEGSVAAPYNGSALRPNLAGIDTTAPNYMQEATVPMGSETHAG
EDVAIYASGPNAYLFRGPQEQNVIYHVMADALGLNKGGHGHGHGRGRD

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=489, Percent_Identity=41.7177914110429, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI157266296, Length=490, Percent_Identity=42.0408163265306, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI94721246, Length=490, Percent_Identity=41.6326530612245, Blast_Score=358, Evalue=6e-99,
Organism=Homo sapiens, GI116734717, Length=481, Percent_Identity=42.4116424116424, Blast_Score=354, Evalue=9e-98,
Organism=Homo sapiens, GI294660770, Length=472, Percent_Identity=41.9491525423729, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI294660772, Length=440, Percent_Identity=39.5454545454545, Blast_Score=299, Evalue=5e-81,
Organism=Escherichia coli, GI48994877, Length=370, Percent_Identity=34.8648648648649, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320689, Length=339, Percent_Identity=34.2182890855457, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI21355149, Length=477, Percent_Identity=43.3962264150943, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI21358067, Length=494, Percent_Identity=41.2955465587045, Blast_Score=345, Evalue=5e-95,
Organism=Drosophila melanogaster, GI19921912, Length=473, Percent_Identity=42.4947145877378, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24585213, Length=509, Percent_Identity=39.2927308447937, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI85725200, Length=495, Percent_Identity=39.1919191919192, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI85815833, Length=495, Percent_Identity=39.1919191919192, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI21355981, Length=494, Percent_Identity=38.4615384615385, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI21355151, Length=495, Percent_Identity=38.5858585858586, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24651554, Length=506, Percent_Identity=39.1304347826087, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24657842, Length=474, Percent_Identity=38.6075949367089, Blast_Score=295, Evalue=5e-80,
Organism=Drosophila melanogaster, GI24657835, Length=473, Percent_Identity=38.4778012684989, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24657827, Length=475, Percent_Identity=37.8947368421053, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24651556, Length=462, Percent_Identity=39.8268398268398, Blast_Score=278, Evalue=8e-75,
Organism=Drosophila melanogaster, GI18859923, Length=484, Percent_Identity=34.297520661157, Blast_Score=245, Evalue=7e-65,
Organism=Drosophila melanogaster, GI24645593, Length=387, Percent_Identity=26.3565891472868, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 56361; Mature: 56361

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPKALAISIALALCAAQAHAAGNAVPTSAEEWFAAGRAAVETSKHVVPNRARAKNVILF
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHCCCCCCCCCCCCEEEEE
VGDGMGVSTVTAARILEGQMRNADGEFNRLSFEKLDHMGTSVTASANQQTSDSAPTATAM
EECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEE
VTGIKTNDGAISVDQTIERNEPSATVTRAKSVKTILEQAEERGMSTGIVTTARLTHATPA
EEEEECCCCCEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCC
VNYAHIGNRDWEADSNLPAGATVADIARQLLEFPYGDGLEVALGGGRSYFMPNTASDPEY
CEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCCCCCC
PSQKGRRKDGRDLTKEWTTKYKQSAYVWDKAAFDAVNPQQTKHLLGLFERSHMRYEADRK
CCHHCCCCCCCHHHHHHHHHHHHHHEEECHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
DDVAGEPSLAEMTEKAIKMLGQNKKGFYLMVEAGRIDHAHHAGNAYRALTDTVALSEAVE
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHH
VAKRLTDDQDTLIVVTADHSHVFTIAGYPSRGNPILGKSAIDGVASTDALGLTYTTVSYA
HHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEEC
NGPGWTGGFQRKEYNPATEGSVAAPYNGSALRPNLAGIDTTAPNYMQEATVPMGSETHAG
CCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
EDVAIYASGPNAYLFRGPQEQNVIYHVMADALGLNKGGHGHGHGRGRD
CCEEEEECCCCEEEECCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQPKALAISIALALCAAQAHAAGNAVPTSAEEWFAAGRAAVETSKHVVPNRARAKNVILF
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHCCCCCCCCCCCCEEEEE
VGDGMGVSTVTAARILEGQMRNADGEFNRLSFEKLDHMGTSVTASANQQTSDSAPTATAM
EECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEE
VTGIKTNDGAISVDQTIERNEPSATVTRAKSVKTILEQAEERGMSTGIVTTARLTHATPA
EEEEECCCCCEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCC
VNYAHIGNRDWEADSNLPAGATVADIARQLLEFPYGDGLEVALGGGRSYFMPNTASDPEY
CEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCCCCCC
PSQKGRRKDGRDLTKEWTTKYKQSAYVWDKAAFDAVNPQQTKHLLGLFERSHMRYEADRK
CCHHCCCCCCCHHHHHHHHHHHHHHEEECHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
DDVAGEPSLAEMTEKAIKMLGQNKKGFYLMVEAGRIDHAHHAGNAYRALTDTVALSEAVE
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHH
VAKRLTDDQDTLIVVTADHSHVFTIAGYPSRGNPILGKSAIDGVASTDALGLTYTTVSYA
HHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEEC
NGPGWTGGFQRKEYNPATEGSVAAPYNGSALRPNLAGIDTTAPNYMQEATVPMGSETHAG
CCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
EDVAIYASGPNAYLFRGPQEQNVIYHVMADALGLNKGGHGHGHGRGRD
CCEEEEECCCCEEEECCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]