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The map label for this gene is def2 [H]

Identifier: 119899899

GI number: 119899899

Start: 3962595

End: 3963128

Strand: Direct

Name: def2 [H]

Synonym: azo3610

Alternate gene names: 119899899

Gene position: 3962595-3963128 (Clockwise)

Preceding gene: 119899898

Following gene: 119899900

Centisome position: 90.55

GC content: 67.6

Gene sequence:

>534_bases
ATGATCCTCGACATCCTCCGCATGGGCGATCCCCGGCTGATGCGCGCTGCGCGCCCGGTCTGCGCGTTCGGTACCGACGA
ACTGCGCCGCATCGTCGCCGACATGGTCGAAACCATGCATGCGGCGGGCGGTGTCGGTCTTGCGGCGCCGCAGGTAGGCA
TCGACCTGCGGCTGGTGATCTTCGGCTTCGAACGCAGCGAACGCTACCCGGACGCGCCCCCGGTGCCCTTCACCGTGCTG
GCCAACCCGGTGCTCACGCCCTTGTCCGACGAACTGGAAGAAGGCTGGGAAGGCTGCCTGTCGGTGCCGGGCCTGCGTGG
CTGGGTGCCGCGCTTCCGTCACCTGCTTTACCGCGGCGCGGACATCGACGGCAAGCCGCTGGAGCGCGAGGTGGAGGGTT
TTCATGCCCGCGTGGTGCAGCACGAGTGCGACCACCTGGACGGTATCCTGTATCCGGCCCGGATCAGCGACTTCAGCCGC
TTCGGCTTCATCGACGCGCTGCCGGCGAACGATTCAGCGGCGGAAGAAGTCTGA

Upstream 100 bases:

>100_bases
GCCTCACGCTGCCGCATGCGCTGGTCCGCCCCCTGCTGGCAGAAGCGCTCTATCGCGCCTGGACCGTCACCCGCAATCAC
CCCTACCACCGCGAATAGCC

Downstream 100 bases:

>100_bases
ACAGACGGTAACGACGAGCCCGCCCGGCCGGCTACAATTGCGCGTCATTCCCCGAATGCCTTTCACATGGCCGCCAGTAC
TCCCCGCATCTACCTCGCCT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MILDILRMGDPRLMRAARPVCAFGTDELRRIVADMVETMHAAGGVGLAAPQVGIDLRLVIFGFERSERYPDAPPVPFTVL
ANPVLTPLSDELEEGWEGCLSVPGLRGWVPRFRHLLYRGADIDGKPLEREVEGFHARVVQHECDHLDGILYPARISDFSR
FGFIDALPANDSAAEEV

Sequences:

>Translated_177_residues
MILDILRMGDPRLMRAARPVCAFGTDELRRIVADMVETMHAAGGVGLAAPQVGIDLRLVIFGFERSERYPDAPPVPFTVL
ANPVLTPLSDELEEGWEGCLSVPGLRGWVPRFRHLLYRGADIDGKPLEREVEGFHARVVQHECDHLDGILYPARISDFSR
FGFIDALPANDSAAEEV
>Mature_177_residues
MILDILRMGDPRLMRAARPVCAFGTDELRRIVADMVETMHAAGGVGLAAPQVGIDLRLVIFGFERSERYPDAPPVPFTVL
ANPVLTPLSDELEEGWEGCLSVPGLRGWVPRFRHLLYRGADIDGKPLEREVEGFHARVVQHECDHLDGILYPARISDFSR
FGFIDALPANDSAAEEV

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=178, Percent_Identity=32.0224719101124, Blast_Score=84, Evalue=7e-17,
Organism=Escherichia coli, GI1789682, Length=153, Percent_Identity=43.1372549019608, Blast_Score=100, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24645728, Length=169, Percent_Identity=36.094674556213, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=35.8974358974359, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19606; Mature: 19606

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILDILRMGDPRLMRAARPVCAFGTDELRRIVADMVETMHAAGGVGLAAPQVGIDLRLVI
CEEEEECCCCHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
FGFERSERYPDAPPVPFTVLANPVLTPLSDELEEGWEGCLSVPGLRGWVPRFRHLLYRGA
EECCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
DIDGKPLEREVEGFHARVVQHECDHLDGILYPARISDFSRFGFIDALPANDSAAEEV
CCCCCCHHHHHHHHHHHHHHHHHHHHCCEECCHHHCCHHHHCCEECCCCCCCCCCCC
>Mature Secondary Structure
MILDILRMGDPRLMRAARPVCAFGTDELRRIVADMVETMHAAGGVGLAAPQVGIDLRLVI
CEEEEECCCCHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
FGFERSERYPDAPPVPFTVLANPVLTPLSDELEEGWEGCLSVPGLRGWVPRFRHLLYRGA
EECCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
DIDGKPLEREVEGFHARVVQHECDHLDGILYPARISDFSRFGFIDALPANDSAAEEV
CCCCCCHHHHHHHHHHHHHHHHHHHHCCEECCHHHCCHHHHCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]