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The map label for this gene is rlmH

Identifier: 119899898

GI number: 119899898

Start: 3962122

End: 3962592

Strand: Direct

Name: rlmH

Synonym: azo3609

Alternate gene names: 119899898

Gene position: 3962122-3962592 (Clockwise)

Preceding gene: 119899897

Following gene: 119899899

Centisome position: 90.54

GC content: 72.4

Gene sequence:

>471_bases
ATGAAGCTGCTCGTGGTGGCGGTCGGCCACCGCATGCCGGCGTGGGTCGATGCCGGTTTCGATGAATTCGCGCGCCGCAT
GCCGCGCGAACTGCCGCTGCAACTGATCGAAGTGAAGGCTGAGCCGCGCACCAGCGGCAAGCCGGTGGAGGCGATGATGG
CGGCGGAAGCTGCCCGCATCGAGGCCGTGCTGCCGCCCCGCTGCCGCCGCGTGGTGCTCGACGAGCGCGGCGGCGACCGC
ACGACGGCTGCGCTGGCGCGCCGCCTGGAGGCCTGGCAGGGCGAGGGCGAGGACGTCGCCTTCATCGTCGGCGGCCCCGA
CGGCCTGGCGCCCTCCCTCAAGGCCAGCGCGGCCGAATCCCTGCGCCTGTCCAGCCTCACGCTGCCGCATGCGCTGGTCC
GCCCCCTGCTGGCAGAAGCGCTCTATCGCGCCTGGACCGTCACCCGCAATCACCCCTACCACCGCGAATAG

Upstream 100 bases:

>100_bases
ACATCATGCAGCCGGCCGTCCGCAGCTATTACAACCTCGAGGAACTCTGGGTCGCGCATCCCGCCAGCCGGCACAAGGCG
GCGGAGGCCCGACAGGGCGA

Downstream 100 bases:

>100_bases
CCATGATCCTCGACATCCTCCGCATGGGCGATCCCCGGCTGATGCGCGCTGCGCGCCCGGTCTGCGCGTTCGGTACCGAC
GAACTGCGCCGCATCGTCGC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKLLVVAVGHRMPAWVDAGFDEFARRMPRELPLQLIEVKAEPRTSGKPVEAMMAAEAARIEAVLPPRCRRVVLDERGGDR
TTAALARRLEAWQGEGEDVAFIVGGPDGLAPSLKASAAESLRLSSLTLPHALVRPLLAEALYRAWTVTRNHPYHRE

Sequences:

>Translated_156_residues
MKLLVVAVGHRMPAWVDAGFDEFARRMPRELPLQLIEVKAEPRTSGKPVEAMMAAEAARIEAVLPPRCRRVVLDERGGDR
TTAALARRLEAWQGEGEDVAFIVGGPDGLAPSLKASAAESLRLSSLTLPHALVRPLLAEALYRAWTVTRNHPYHRE
>Mature_156_residues
MKLLVVAVGHRMPAWVDAGFDEFARRMPRELPLQLIEVKAEPRTSGKPVEAMMAAEAARIEAVLPPRCRRVVLDERGGDR
TTAALARRLEAWQGEGEDVAFIVGGPDGLAPSLKASAAESLRLSSLTLPHALVRPLLAEALYRAWTVTRNHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=48.0769230769231, Blast_Score=150, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_AZOSB (A1KBL9)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_935111.1
- ProteinModelPortal:   A1KBL9
- SMR:   A1KBL9
- STRING:   A1KBL9
- GeneID:   4608035
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3609
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- PhylomeDB:   A1KBL9
- ProtClustDB:   PRK00103
- BioCyc:   ASP62928:AZO3609-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17194; Mature: 17194

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVVAVGHRMPAWVDAGFDEFARRMPRELPLQLIEVKAEPRTSGKPVEAMMAAEAARI
CEEEEEECCCCCCHHHHCCHHHHHHHCCHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
EAVLPPRCRRVVLDERGGDRTTAALARRLEAWQGEGEDVAFIVGGPDGLAPSLKASAAES
HCCCCHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHH
LRLSSLTLPHALVRPLLAEALYRAWTVTRNHPYHRE
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLLVVAVGHRMPAWVDAGFDEFARRMPRELPLQLIEVKAEPRTSGKPVEAMMAAEAARI
CEEEEEECCCCCCHHHHCCHHHHHHHCCHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
EAVLPPRCRRVVLDERGGDRTTAALARRLEAWQGEGEDVAFIVGGPDGLAPSLKASAAES
HCCCCHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHH
LRLSSLTLPHALVRPLLAEALYRAWTVTRNHPYHRE
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA