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The map label for this gene is rfaD

Identifier: 119899853

GI number: 119899853

Start: 3910439

End: 3911416

Strand: Direct

Name: rfaD

Synonym: azo3564

Alternate gene names: 119899853

Gene position: 3910439-3911416 (Clockwise)

Preceding gene: 119899852

Following gene: 119899854

Centisome position: 89.36

GC content: 66.16

Gene sequence:

>978_bases
ATGATCATCGTTACCGGCGGCGCCGGCTTCATCGGCAGCAACATCGTGCAGGGCCTCAACGACCGTGGCATCACCGACAT
CCTGGTGGTGGACGACCTGCGCGACGGGCGCAAGTGCCTCAACCTGGCCGATGCCGACATCCGCGACTACATGGACATGG
ACGACTTCCTCGCCCGCATCCAGACCGGGGAGGACCTCGGCCGCGTCGAGGCGGTGTTCCACGAGGGCGCGTGCTCGTCC
ACCACCGAATGGGACGGCCGCTTCGTGATGCGGGTGAACTACGAGTACACCAAGGCCCTGCTGGCCTGGTGCACCCAGCG
CAAGATCCCGCTGATCTACGCCTCGTCGGCCTCGGTCTACGGCATGGGCCCCACCTTCCGCGAGGCGCGCGAGTTCGAGC
AGCCGCTCAACATGTACGCCTACTCCAAGTTCCTGTTCGACTGCCATCTGCGCGCGCAGCAAGGCATCGAAAGCCAGGTC
GTCGGGCTGCGCTATTTCAACGTCTATGGCCCGCGCGAACAGCACAAGGGCTCGATGGCGAGCGTCGCCTACCACTTCAA
CAACCAGTTGAATGAATCCGGCCGGGTGCGCCTGTTCGAAGGCTCCGACGGCTATGGCCCGGGCGAGCAGCGGCGCGACT
TCATCCATGTGGACGACGTGGTCGCGGTCAACCTGTGGCTGTTCGACAACCCGCAGGTGTCCGGCATCTACAACCTCGGC
ACCGGGCGCGCACAAAGCTTCAACGACGTCGCGCGCGCGGCGATCAAGTGGTTCCGCGCGCAGGGCGAGGACGGCGGCCG
CGAGCGCGGCGGCATCGACTACATCCCCTTCCCCGACCACCTCAAGGGCCGCTACCAGAGCTTCACCGAAGCCGACATGG
GCGCGCTGCGCGCCGCCGGCTACGACCGTCCCTTCATGGACGTCGAAACCGGCGTGCCGGCCTACCTGAGCTGGCTGGCG
AGCCAGGACAAGCGTTGA

Upstream 100 bases:

>100_bases
GCTGGGCACCGCCACGGTCAGCGCCGTCGAACTCGCCGATGCCCTGCGCGCCGCCTGAGGCGCCCGCGGCCCCCAGACGT
ACTGAAAGAGGAAAACCGAG

Downstream 100 bases:

>100_bases
GCCGCTCCCGCAAGATTCTCGTCGTCGGCCCCTCGTGGGTGGGCGACATGGTGATGGCGCAGAGCCTGTTCGCGCTGCTG
CAGCGCGGCGGGCCGAACGC

Product: putative ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MIIVTGGAGFIGSNIVQGLNDRGITDILVVDDLRDGRKCLNLADADIRDYMDMDDFLARIQTGEDLGRVEAVFHEGACSS
TTEWDGRFVMRVNYEYTKALLAWCTQRKIPLIYASSASVYGMGPTFREAREFEQPLNMYAYSKFLFDCHLRAQQGIESQV
VGLRYFNVYGPREQHKGSMASVAYHFNNQLNESGRVRLFEGSDGYGPGEQRRDFIHVDDVVAVNLWLFDNPQVSGIYNLG
TGRAQSFNDVARAAIKWFRAQGEDGGRERGGIDYIPFPDHLKGRYQSFTEADMGALRAAGYDRPFMDVETGVPAYLSWLA
SQDKR

Sequences:

>Translated_325_residues
MIIVTGGAGFIGSNIVQGLNDRGITDILVVDDLRDGRKCLNLADADIRDYMDMDDFLARIQTGEDLGRVEAVFHEGACSS
TTEWDGRFVMRVNYEYTKALLAWCTQRKIPLIYASSASVYGMGPTFREAREFEQPLNMYAYSKFLFDCHLRAQQGIESQV
VGLRYFNVYGPREQHKGSMASVAYHFNNQLNESGRVRLFEGSDGYGPGEQRRDFIHVDDVVAVNLWLFDNPQVSGIYNLG
TGRAQSFNDVARAAIKWFRAQGEDGGRERGGIDYIPFPDHLKGRYQSFTEADMGALRAAGYDRPFMDVETGVPAYLSWLA
SQDKR
>Mature_325_residues
MIIVTGGAGFIGSNIVQGLNDRGITDILVVDDLRDGRKCLNLADADIRDYMDMDDFLARIQTGEDLGRVEAVFHEGACSS
TTEWDGRFVMRVNYEYTKALLAWCTQRKIPLIYASSASVYGMGPTFREAREFEQPLNMYAYSKFLFDCHLRAQQGIESQV
VGLRYFNVYGPREQHKGSMASVAYHFNNQLNESGRVRLFEGSDGYGPGEQRRDFIHVDDVVAVNLWLFDNPQVSGIYNLG
TGRAQSFNDVARAAIKWFRAQGEDGGRERGGIDYIPFPDHLKGRYQSFTEADMGALRAAGYDRPFMDVETGVPAYLSWLA
SQDKR

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily

Homologues:

Organism=Escherichia coli, GI1790049, Length=322, Percent_Identity=60.2484472049689, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): HLDD_AZOSB (A1KBH4)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_935066.1
- ProteinModelPortal:   A1KBH4
- SMR:   A1KBH4
- STRING:   A1KBH4
- GeneID:   4610104
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3564
- eggNOG:   COG0451
- HOGENOM:   HBG755066
- OMA:   GFIGSAM
- PhylomeDB:   A1KBH4
- ProtClustDB:   CLSK766742
- BioCyc:   ASP62928:AZO3564-MONOMER
- HAMAP:   MF_01601
- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10366:SF29
- TIGRFAMs:   TIGR02197

Pfam domain/function: PF01370 Epimerase

EC number: =5.1.3.20

Molecular weight: Translated: 36592; Mature: 36592

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: ACT_SITE 139-139 ACT_SITE 176-176 BINDING 38-38 BINDING 143-143 BINDING 167-167 BINDING 168-168 BINDING 176-176 BINDING 178-178 BINDING 185-185 BINDING 212-212 BINDING 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVTGGAGFIGSNIVQGLNDRGITDILVVDDLRDGRKCLNLADADIRDYMDMDDFLARI
CEEEECCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCCHHHHHCCHHHHHHHH
QTGEDLGRVEAVFHEGACSSTTEWDGRFVMRVNYEYTKALLAWCTQRKIPLIYASSASVY
HCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCCEEEECCCCEE
GMGPTFREAREFEQPLNMYAYSKFLFDCHLRAQQGIESQVVGLRYFNVYGPREQHKGSMA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCCHHHHCCCHH
SVAYHFNNQLNESGRVRLFEGSDGYGPGEQRRDFIHVDDVVAVNLWLFDNPQVSGIYNLG
EEEEECCCCCCCCCCEEEEECCCCCCCCHHCCCEEEECCEEEEEEEEECCCCCCEEEECC
TGRAQSFNDVARAAIKWFRAQGEDGGRERGGIDYIPFPDHLKGRYQSFTEADMGALRAAG
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
YDRPFMDVETGVPAYLSWLASQDKR
CCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIIVTGGAGFIGSNIVQGLNDRGITDILVVDDLRDGRKCLNLADADIRDYMDMDDFLARI
CEEEECCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCCHHHHHCCHHHHHHHH
QTGEDLGRVEAVFHEGACSSTTEWDGRFVMRVNYEYTKALLAWCTQRKIPLIYASSASVY
HCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCCEEEECCCCEE
GMGPTFREAREFEQPLNMYAYSKFLFDCHLRAQQGIESQVVGLRYFNVYGPREQHKGSMA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCCHHHHCCCHH
SVAYHFNNQLNESGRVRLFEGSDGYGPGEQRRDFIHVDDVVAVNLWLFDNPQVSGIYNLG
EEEEECCCCCCCCCCEEEEECCCCCCCCHHCCCEEEECCEEEEEEEEECCCCCCEEEECC
TGRAQSFNDVARAAIKWFRAQGEDGGRERGGIDYIPFPDHLKGRYQSFTEADMGALRAAG
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
YDRPFMDVETGVPAYLSWLASQDKR
CCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA