Click here to switch to the map view.

The map label for this gene is btuB [H]

Identifier: 119899844

GI number: 119899844

Start: 3899200

End: 3901071

Strand: Reverse

Name: btuB [H]

Synonym: azo3555

Alternate gene names: 119899844

Gene position: 3901071-3899200 (Counterclockwise)

Preceding gene: 119899845

Following gene: 119899843

Centisome position: 89.15

GC content: 68.0

Gene sequence:

>1872_bases
ATGACGCTTCGCCTGACCACCCTTGCGCTCGCCCTTGCCGCGACCGCGCCGCTGTTTCCCCTGCCCACTTTTGCCGCTGA
CAATGCCGCGCTCGACGAGGTCGTGGTGACCGCCACCCGCCAGCAGCGCCGCGCCGAGGACAGCCTCGCCAGCGTCGAGG
TGCTCGAACGCGCCGACATCGAGCGCGCCGGCCATTCCTCGCTGATCGAGGTGTTGCGGGCGATGCCTGGCGTGCAGGTC
ACCGCCAACGGCGGCGCCGGCGCCAACGCCAACGTCTATATCCGCGGTGCCGAGGCGCGCCACACGCTGCTGTTGATCGA
CGGCGTGCGCGTCGGCTCGGCCACCTCCGGCGCCGCTACCTTCGAAACCATCCCGCTGGCGATGATCGAACGCATCGAAG
TGCTGCGCGGGCCGGCGAGCGCGCTCTACGGCTCGGAAGCGATCGGCGGCGTGATCCAGGTGTTCACCCGCCGCGGCGAG
GCCGGCTTCCACCCCGAGGTCTATGTCGGCTACGGCACCGACGACACCTTCAAGGGCTCGGCGACGCTCGCCGGTGGCGT
CAACCGGCTGCGCTACAGCCTCACCGCCGGCAAGGAGCGCACCGACGGCTTCAATTCCAAGCGCGATCCGGCCTACTGGC
GCAGCGGCGCCCGCACCTCGTACTGGGCGGACGACGACGGCTATCGCAACGACATGCTGAGCGGCGCGCTGTCGCTGGGT
TTCCGCGAGCGCGACGAGGTCGGGCTGAGCTTCACCCAGTCGGATGCACGCAACCGCTACGACGCCTCGGCGCTGCGCTA
CTTCGATTCCTACCTCGACAAGAAGGCTCGCAGCGTCGGCGTGTACATGCGCAACCAGCTCGCCAGTGGCTGGACCAGCA
CGCTGCGCCTCGGCCAGAGCGAGGACAAGTCGCTGAACAAGGCGAGCAGCGCCGCGCCCAGCTTCTTCAACACCCTGCAG
GACCAGCTGATGTGGCAGCACGACGTCGACCTGCCGCTCGGCCGCCTGCTGCTCGCCTATGAATACGTCGAAACCAGGCT
CGACAGCTCGACCGCCTACCGCGAGGACAGCCGGCGGGTGAATTCCGCGCTGCTCGGCTGGACCGCCGATCTCGACGCCC
ACCATTTGCAGCTCAACGCCCGTCACGACGACAATTCGCAATTTGGCGGCAAGACCACCGGGATGCTGGCCTACGGCTAC
GACCTCGCCGCCGGGCTGCGCCTGCGCGGCAGTATCGCCACCGCGTTCAACGCGCCGACCTTCAACCAGCTCTACTGGCC
GATGACCTCGCTCACCAGCTACCACGGCAACCCCGACCTCAAGCCGGAGGAAGCGCTCAACCGCGAGCTGGGCTTGCGTT
GGAGCCAGGGCAGCCATATGGTCGAGCTGACCTATTTCGACAACAAGGTGAAGAACCTGATTGCCAGCAATCCCACGCCC
GCGCTGCGCGGCCAGCAGATCAATATCGGCGAGGCCCGGCTGAAGGGGGTCGAACTCGGCTACCTGCTCGCGCTCGGCGA
CTTCAAGCTCGGCGCCGGGGTCGATTTCCTCAGCGCCAAGGACGAAGACACCGGCCTGCGGCTGCCCCGGCGCGCGCAGC
GCACCGGCTACCTGCGCCTGTCGCAGACGCTGGAGCGCTTCGAATGGGGCGTCGACATCGACGGCGCCGGCCGCCGCTAC
GACGACGCGGCCAACACCACGGTGCTGCACGGCTACGGCACGGTCAATGCCTATGCCCATTACCGCGTCGCGCCGGACTG
GAAGGTGGAACTGCGCGCCAACAACCTCCTCGACAAGGACTACGAGCTTGCACGGGGATATCGCACGCCGGGTGCCAGTG
CCTTCATTGCCGTCCGCTACGCGCCGCGCTGA

Upstream 100 bases:

>100_bases
GGGCGCCCGCTTCTCGTTCTGAGCGCGCGCCGTCGCCGCATTCCTTTTTGCGCCCGGCGTGCTTCGCCGCCGGGCGCCAA
CGACCTGTTCCCTACCGATC

Downstream 100 bases:

>100_bases
GCCTGCATGGCCGTCGGATTGCCCCTTGCCGCGACTGATGCGGGCCCGCGCATGAGCAGCGTGCGGCGGCGCGCATGGAT
CTGCGCGGGCGGACTGCTCG

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MTLRLTTLALALAATAPLFPLPTFAADNAALDEVVVTATRQQRRAEDSLASVEVLERADIERAGHSSLIEVLRAMPGVQV
TANGGAGANANVYIRGAEARHTLLLIDGVRVGSATSGAATFETIPLAMIERIEVLRGPASALYGSEAIGGVIQVFTRRGE
AGFHPEVYVGYGTDDTFKGSATLAGGVNRLRYSLTAGKERTDGFNSKRDPAYWRSGARTSYWADDDGYRNDMLSGALSLG
FRERDEVGLSFTQSDARNRYDASALRYFDSYLDKKARSVGVYMRNQLASGWTSTLRLGQSEDKSLNKASSAAPSFFNTLQ
DQLMWQHDVDLPLGRLLLAYEYVETRLDSSTAYREDSRRVNSALLGWTADLDAHHLQLNARHDDNSQFGGKTTGMLAYGY
DLAAGLRLRGSIATAFNAPTFNQLYWPMTSLTSYHGNPDLKPEEALNRELGLRWSQGSHMVELTYFDNKVKNLIASNPTP
ALRGQQINIGEARLKGVELGYLLALGDFKLGAGVDFLSAKDEDTGLRLPRRAQRTGYLRLSQTLERFEWGVDIDGAGRRY
DDAANTTVLHGYGTVNAYAHYRVAPDWKVELRANNLLDKDYELARGYRTPGASAFIAVRYAPR

Sequences:

>Translated_623_residues
MTLRLTTLALALAATAPLFPLPTFAADNAALDEVVVTATRQQRRAEDSLASVEVLERADIERAGHSSLIEVLRAMPGVQV
TANGGAGANANVYIRGAEARHTLLLIDGVRVGSATSGAATFETIPLAMIERIEVLRGPASALYGSEAIGGVIQVFTRRGE
AGFHPEVYVGYGTDDTFKGSATLAGGVNRLRYSLTAGKERTDGFNSKRDPAYWRSGARTSYWADDDGYRNDMLSGALSLG
FRERDEVGLSFTQSDARNRYDASALRYFDSYLDKKARSVGVYMRNQLASGWTSTLRLGQSEDKSLNKASSAAPSFFNTLQ
DQLMWQHDVDLPLGRLLLAYEYVETRLDSSTAYREDSRRVNSALLGWTADLDAHHLQLNARHDDNSQFGGKTTGMLAYGY
DLAAGLRLRGSIATAFNAPTFNQLYWPMTSLTSYHGNPDLKPEEALNRELGLRWSQGSHMVELTYFDNKVKNLIASNPTP
ALRGQQINIGEARLKGVELGYLLALGDFKLGAGVDFLSAKDEDTGLRLPRRAQRTGYLRLSQTLERFEWGVDIDGAGRRY
DDAANTTVLHGYGTVNAYAHYRVAPDWKVELRANNLLDKDYELARGYRTPGASAFIAVRYAPR
>Mature_622_residues
TLRLTTLALALAATAPLFPLPTFAADNAALDEVVVTATRQQRRAEDSLASVEVLERADIERAGHSSLIEVLRAMPGVQVT
ANGGAGANANVYIRGAEARHTLLLIDGVRVGSATSGAATFETIPLAMIERIEVLRGPASALYGSEAIGGVIQVFTRRGEA
GFHPEVYVGYGTDDTFKGSATLAGGVNRLRYSLTAGKERTDGFNSKRDPAYWRSGARTSYWADDDGYRNDMLSGALSLGF
RERDEVGLSFTQSDARNRYDASALRYFDSYLDKKARSVGVYMRNQLASGWTSTLRLGQSEDKSLNKASSAAPSFFNTLQD
QLMWQHDVDLPLGRLLLAYEYVETRLDSSTAYREDSRRVNSALLGWTADLDAHHLQLNARHDDNSQFGGKTTGMLAYGYD
LAAGLRLRGSIATAFNAPTFNQLYWPMTSLTSYHGNPDLKPEEALNRELGLRWSQGSHMVELTYFDNKVKNLIASNPTPA
LRGQQINIGEARLKGVELGYLLALGDFKLGAGVDFLSAKDEDTGLRLPRRAQRTGYLRLSQTLERFEWGVDIDGAGRRYD
DAANTTVLHGYGTVNAYAHYRVAPDWKVELRANNLLDKDYELARGYRTPGASAFIAVRYAPR

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=628, Percent_Identity=29.140127388535, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI1788478, Length=691, Percent_Identity=27.0622286541245, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786798, Length=149, Percent_Identity=36.9127516778523, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787723, Length=690, Percent_Identity=25.3623188405797, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1790743, Length=191, Percent_Identity=27.2251308900524, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68464; Mature: 68333

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRLTTLALALAATAPLFPLPTFAADNAALDEVVVTATRQQRRAEDSLASVEVLERADI
CEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERAGHSSLIEVLRAMPGVQVTANGGAGANANVYIRGAEARHTLLLIDGVRVGSATSGAAT
HHCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCEEEEEEECEEECCCCCCCCH
FETIPLAMIERIEVLRGPASALYGSEAIGGVIQVFTRRGEAGFHPEVYVGYGTDDTFKGS
HHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCC
ATLAGGVNRLRYSLTAGKERTDGFNSKRDPAYWRSGARTSYWADDDGYRNDMLSGALSLG
EEECCCHHHEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
FRERDEVGLSFTQSDARNRYDASALRYFDSYLDKKARSVGVYMRNQLASGWTSTLRLGQS
CCCCCCCCCEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
EDKSLNKASSAAPSFFNTLQDQLMWQHDVDLPLGRLLLAYEYVETRLDSSTAYREDSRRV
CCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NSALLGWTADLDAHHLQLNARHDDNSQFGGKTTGMLAYGYDLAAGLRLRGSIATAFNAPT
HHHHEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHCCEEEECCEEEEECCCC
FNQLYWPMTSLTSYHGNPDLKPEEALNRELGLRWSQGSHMVELTYFDNKVKNLIASNPTP
CCHHHCCHHHHHHCCCCCCCCHHHHHHHHHCCEECCCCCEEEEEECCHHHHHHHHCCCCC
ALRGQQINIGEARLKGVELGYLLALGDFKLGAGVDFLSAKDEDTGLRLPRRAQRTGYLRL
CCCCCEECCCHHHHCCEEEEEEEEECCEECCCCCEEECCCCCCCCCCCCCHHHHCCHHHH
SQTLERFEWGVDIDGAGRRYDDAANTTVLHGYGTVNAYAHYRVAPDWKVELRANNLLDKD
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEEEECCCCEEEEECCCCCCCC
YELARGYRTPGASAFIAVRYAPR
HHHHCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TLRLTTLALALAATAPLFPLPTFAADNAALDEVVVTATRQQRRAEDSLASVEVLERADI
EEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERAGHSSLIEVLRAMPGVQVTANGGAGANANVYIRGAEARHTLLLIDGVRVGSATSGAAT
HHCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCEEEEEEECEEECCCCCCCCH
FETIPLAMIERIEVLRGPASALYGSEAIGGVIQVFTRRGEAGFHPEVYVGYGTDDTFKGS
HHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCC
ATLAGGVNRLRYSLTAGKERTDGFNSKRDPAYWRSGARTSYWADDDGYRNDMLSGALSLG
EEECCCHHHEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
FRERDEVGLSFTQSDARNRYDASALRYFDSYLDKKARSVGVYMRNQLASGWTSTLRLGQS
CCCCCCCCCEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
EDKSLNKASSAAPSFFNTLQDQLMWQHDVDLPLGRLLLAYEYVETRLDSSTAYREDSRRV
CCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NSALLGWTADLDAHHLQLNARHDDNSQFGGKTTGMLAYGYDLAAGLRLRGSIATAFNAPT
HHHHEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHCCEEEECCEEEEECCCC
FNQLYWPMTSLTSYHGNPDLKPEEALNRELGLRWSQGSHMVELTYFDNKVKNLIASNPTP
CCHHHCCHHHHHHCCCCCCCCHHHHHHHHHCCEECCCCCEEEEEECCHHHHHHHHCCCCC
ALRGQQINIGEARLKGVELGYLLALGDFKLGAGVDFLSAKDEDTGLRLPRRAQRTGYLRL
CCCCCEECCCHHHHCCEEEEEEEEECCEECCCCCEEECCCCCCCCCCCCCHHHHCCHHHH
SQTLERFEWGVDIDGAGRRYDDAANTTVLHGYGTVNAYAHYRVAPDWKVELRANNLLDKD
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEEEECCCCEEEEECCCCCCCC
YELARGYRTPGASAFIAVRYAPR
HHHHCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA