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The map label for this gene is cobB [H]

Identifier: 119899839

GI number: 119899839

Start: 3894532

End: 3895827

Strand: Reverse

Name: cobB [H]

Synonym: azo3550

Alternate gene names: 119899839

Gene position: 3895827-3894532 (Counterclockwise)

Preceding gene: 119899840

Following gene: 119899838

Centisome position: 89.03

GC content: 73.23

Gene sequence:

>1296_bases
TTGAGCGCCGCACGCCGCTGCCCCGCGCTGTTGGTCGCCGCGCCGGCCTCGGGCCAGGGCAAGACCACGGTCACCGCGGC
GCTTGCCCGGCTGCACACCCGGCTCGGGCGCCGCGTGCGGGTGTTCAAGTGCGGGCCGGACTTCCTCGACCCGCAGATCC
ACGCGGTGGCGAGCGGGGCGCCGGTGTACAACGTCGATCTCGGCATGTGCGGCGAGGCCGACATCGCGGCGCGGCTCTAC
GCCGCGGCGGGCGAGGCCGACCTGATCCTGGTCGAAGGCGTCATGGGCCTCTACGACGGCGCACCCTCTGGCGCGGACAT
CGCCTGCCGCTTCGGCATTCCGGTGATGGCGGTGATCGACGCGCGCGCGATGGCGCAGACCTTCGGCGCGATCGCACACG
GGCTGGCCACCTTCCGCGCCGGCCTGCCGTTTGCCGGCGTGCTTGCCAATCACGTAGGCAGCGAGCGCCATGCCGACATC
CTGCGCGCGGCCTTGCCGGACGGCATGCGCTGGTTCGGCGCGGTGCTGCGCGATGCGGAGGCGGCGCTGCCCGAACGCTA
TCTGGGCCTGATGCAGGCGGCCGAGATCGACGACCTCGGAACCCGGCTCGATCGCATGGCCGACAGCCTGGCGCGCACCG
GCGCCGCCGAGCTGCCGGCCGCGGTCGAGTTTCCGTCCGCCCCGGCGGAAGCGCTTCCGCCGCTGCTCGCGGGGCGCCGC
ATCGCCATTGCGCGCGACGCCGCCTACGGCTTCATCTACCCGGCCAATGTCGACACGCTACGCGGGCTGGGCGCCGAGCT
GGCCTTCTTCTCGCCGCTTGCCGCCGATCCGCTGCCGGCCTGCGACGCGGTCTGGCTGCCGGGCGGCTATCCCGAACTGC
ATGGCGCGGCGCTCGCCGCCAACACCGGCTTCTGGTCTGCCCTGCGCGCGCACGCCGACGCTGGCCGGCCGCTGCTGGCC
GAATGCGGCGGCATGATGAGCCTGTTCGACGCGGTGGTGGACAAGGCCGGCGCCGAGCACCGCTTCGGCGGACTGCTGCC
GGGACGCGCGGTGATGCAGAAGCGGCTCGCCGCGCTCGGCATGCAGGAAGCCGAGCTGCCGGAAGGGCGGCTCAGCGGCC
ACACCTTCCATTATTCGAAGAGCGAGACGCCGCTAGCGCCGCTGCTGCGCGCGCGGCGCCCCGATGGCGGCGAGGGCGAA
GCGATCTACCGCCGCGCTTCGGTCACCGCCTCGTACGTGCATTTCTACTTCCCGTCCAATCCGGTCGCGGTGGCGCGCCT
GTTCGGCGCCGGCTGA

Upstream 100 bases:

>100_bases
GCGAACCGCATCTGCCGCATGCCGGCTGCAGCGCGGTGCATGCCGGGCAGGTCGCGGAGGGCCTCGACGGCGACGCCGCG
GCCGGGCGCGGGGACGCGTG

Downstream 100 bases:

>100_bases
GGCGGGGGGCGGGGAACCGCGCGGGGTGCGGCGCGCTCCCACCTGCGGATGCGCCCGGATGCGGGCCGCATCGCCGCTAT
AATCCGCCGCTTCGTCCAGC

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSAARRCPALLVAAPASGQGKTTVTAALARLHTRLGRRVRVFKCGPDFLDPQIHAVASGAPVYNVDLGMCGEADIAARLY
AAAGEADLILVEGVMGLYDGAPSGADIACRFGIPVMAVIDARAMAQTFGAIAHGLATFRAGLPFAGVLANHVGSERHADI
LRAALPDGMRWFGAVLRDAEAALPERYLGLMQAAEIDDLGTRLDRMADSLARTGAAELPAAVEFPSAPAEALPPLLAGRR
IAIARDAAYGFIYPANVDTLRGLGAELAFFSPLAADPLPACDAVWLPGGYPELHGAALAANTGFWSALRAHADAGRPLLA
ECGGMMSLFDAVVDKAGAEHRFGGLLPGRAVMQKRLAALGMQEAELPEGRLSGHTFHYSKSETPLAPLLRARRPDGGEGE
AIYRRASVTASYVHFYFPSNPVAVARLFGAG

Sequences:

>Translated_431_residues
MSAARRCPALLVAAPASGQGKTTVTAALARLHTRLGRRVRVFKCGPDFLDPQIHAVASGAPVYNVDLGMCGEADIAARLY
AAAGEADLILVEGVMGLYDGAPSGADIACRFGIPVMAVIDARAMAQTFGAIAHGLATFRAGLPFAGVLANHVGSERHADI
LRAALPDGMRWFGAVLRDAEAALPERYLGLMQAAEIDDLGTRLDRMADSLARTGAAELPAAVEFPSAPAEALPPLLAGRR
IAIARDAAYGFIYPANVDTLRGLGAELAFFSPLAADPLPACDAVWLPGGYPELHGAALAANTGFWSALRAHADAGRPLLA
ECGGMMSLFDAVVDKAGAEHRFGGLLPGRAVMQKRLAALGMQEAELPEGRLSGHTFHYSKSETPLAPLLRARRPDGGEGE
AIYRRASVTASYVHFYFPSNPVAVARLFGAG
>Mature_430_residues
SAARRCPALLVAAPASGQGKTTVTAALARLHTRLGRRVRVFKCGPDFLDPQIHAVASGAPVYNVDLGMCGEADIAARLYA
AAGEADLILVEGVMGLYDGAPSGADIACRFGIPVMAVIDARAMAQTFGAIAHGLATFRAGLPFAGVLANHVGSERHADIL
RAALPDGMRWFGAVLRDAEAALPERYLGLMQAAEIDDLGTRLDRMADSLARTGAAELPAAVEFPSAPAEALPPLLAGRRI
AIARDAAYGFIYPANVDTLRGLGAELAFFSPLAADPLPACDAVWLPGGYPELHGAALAANTGFWSALRAHADAGRPLLAE
CGGMMSLFDAVVDKAGAEHRFGGLLPGRAVMQKRLAALGMQEAELPEGRLSGHTFHYSKSETPLAPLLRARRPDGGEGEA
IYRRASVTASYVHFYFPSNPVAVARLFGAG

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 45055; Mature: 44924

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAARRCPALLVAAPASGQGKTTVTAALARLHTRLGRRVRVFKCGPDFLDPQIHAVASGA
CCCCHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHEEECCC
PVYNVDLGMCGEADIAARLYAAAGEADLILVEGVMGLYDGAPSGADIACRFGIPVMAVID
CEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCCEEEECCCCCHHHHH
ARAMAQTFGAIAHGLATFRAGLPFAGVLANHVGSERHADILRAALPDGMRWFGAVLRDAE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
AALPERYLGLMQAAEIDDLGTRLDRMADSLARTGAAELPAAVEFPSAPAEALPPLLAGRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHCCHHHCCCE
IAIARDAAYGFIYPANVDTLRGLGAELAFFSPLAADPLPACDAVWLPGGYPELHGAALAA
EEEEECCCCEEEECCCCHHHHHCCCHHHHHCCCCCCCCCCCCEEECCCCCCHHCCCEEEC
NTGFWSALRAHADAGRPLLAECGGMMSLFDAVVDKAGAEHRFGGLLPGRAVMQKRLAALG
CCCHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
MQEAELPEGRLSGHTFHYSKSETPLAPLLRARRPDGGEGEAIYRRASVTASYVHFYFPSN
CCHHCCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCEEHHHHCEEEEEEEECCCC
PVAVARLFGAG
HHHHHHHHCCC
>Mature Secondary Structure 
SAARRCPALLVAAPASGQGKTTVTAALARLHTRLGRRVRVFKCGPDFLDPQIHAVASGA
CCCHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHEEECCC
PVYNVDLGMCGEADIAARLYAAAGEADLILVEGVMGLYDGAPSGADIACRFGIPVMAVID
CEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCCEEEECCCCCHHHHH
ARAMAQTFGAIAHGLATFRAGLPFAGVLANHVGSERHADILRAALPDGMRWFGAVLRDAE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
AALPERYLGLMQAAEIDDLGTRLDRMADSLARTGAAELPAAVEFPSAPAEALPPLLAGRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHCCHHHCCCE
IAIARDAAYGFIYPANVDTLRGLGAELAFFSPLAADPLPACDAVWLPGGYPELHGAALAA
EEEEECCCCEEEECCCCHHHHHCCCHHHHHCCCCCCCCCCCCEEECCCCCCHHCCCEEEC
NTGFWSALRAHADAGRPLLAECGGMMSLFDAVVDKAGAEHRFGGLLPGRAVMQKRLAALG
CCCHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
MQEAELPEGRLSGHTFHYSKSETPLAPLLRARRPDGGEGEAIYRRASVTASYVHFYFPSN
CCHHCCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCEEHHHHCEEEEEEEECCCC
PVAVARLFGAG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA