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The map label for this gene is leuA2

Identifier: 119899812

GI number: 119899812

Start: 3866142

End: 3867860

Strand: Reverse

Name: leuA2

Synonym: azo3523

Alternate gene names: 119899812

Gene position: 3867860-3866142 (Counterclockwise)

Preceding gene: 119899813

Following gene: 119899810

Centisome position: 88.39

GC content: 67.66

Gene sequence:

>1719_bases
ATGTTGAAGAACCCGCACACCAAGTACCGTCCCGCCAACCCCTTTGCCGGCGTCGTGCCCGGCGGCCGTGGCCTTAGCGA
CCGCACCTGGCCTGACAACACGATCACGCGCCCGCCGGTGTGGATGAGCACCGACCTGCGCGACGGCAACCAGGCGCTGT
TCGAGCCGATGAACAGCGAGCGCAAGATGCGCATGTTCAAGATGCTGGTGGAGATCGGCCTCAAGGAAATCGAGGTCGCC
TTTCCCGCCGCGTCGCAGACCGACTTCGACTTCGTGCGCGAACTCATCGAAGGCGGCCACATTCCCGACGACGTCACCAT
CGAGGTGCTCACCCAGGCGCGTCCGCACCTCATCGAGCGCACCTTCGAATCGGTGCGCGGCGCCAAGCGCGCCATCGTGC
ACGTCTATAACGCGGTGGCACCCAACTTCCGCCGCATCGTGTTCGACACCGACCGCGCCGGCGTCAAGCAGATCGCGGTC
GACAGCGCGCGCTATTTCGTCGAAATGGCGGCGCGCCAGCCCGGCACCGACTTCACCTTCCAGTACAGCCCCGAGGTGTT
CTCCGGCACCGAACTCGACTTCGCGGTCGAGGTTGCCAACGCGGTGATCGAGGTGTGGCAGCCGACTCCGCAGCAGAAAT
GCATCATCAACCTGCCGGCGACGGTGGAGATGAGCACCCCCAACGTCTACGCCGACCAGATCGAGTGGATGCACCGCCAC
CTCGCCCGGCGCGACAGCGTGCTGCTCTCGGTGCACCCGCACAACGACCGCGGCACCGCGGTGGCCGCCGCCGAGCTGGC
GGTGATGGCCGGCGCCGACCGCGTCGAGGGTTGCCTGTTCGGCAACGGCGAGCGCACCGGCAACGTCGACCTCGTCACCC
TGGCGCTCAATCTCTACAGCCAGGGCGTGCATCCGGGGCTGGACTTCTCGCGCATCAACGAGGTCGCGCGCACGGTGGAG
CACTGCACCCAGTTGCCGATCCATCCGCGCCACCCCTATGTCGGCGATCTCGTGTTCACCGCGTTTTCCGGATCGCACCA
GGATGCGATCAAGAAGGGCTTCGCCGTGCAGCAGCCGGACGCGGTGTGGGAGGTGCCCTATCTGCCAGTGGATCCGGCCG
ACCTCGGCCGCAGCTACGAATCCATCATCCGGGTGAACAGCCAGTCGGGCAAAGGCGGCATCGCCTACCTGCTCGAAGCC
GAATACGGCCTGGTGATGCCGCGCCGCCTGCAGGTGGAGTTCTCGGCGGCGATCCAGCGCATCACCGACGAACGCGGTAC
TGAACTGAGCGCGGGCGACATCTGGCGTGCGTTCGAGGAAGAGTACCTGGCGGTGGCTGCGCCCTATGCCTACGTCGAGC
ATCACCTCGCCGAGCACGGTGGCCAGCAGGGTATCAGCCTGACGGTGGAAGAAGCGGGCGTGCGGCGGGTGTTGCGCGGT
GTCGGCAACGGCCCCATCGACGCCGCGCTGCATGCGCTCGACGCCGGTGCGGTATTGCTCGGCTATGAGGAGCGCGCGAT
CGGCCAGGGCGGCGATGCACGCGCGGTCGCCTACATCGAGTTGGCCGACGGCGAGGGCGGCGGCAGCACCTTCGGCGTCG
GCATCCACGCCAACATCGTCACCGCCTCGGTGCTCGCCATCGTGTCGGCGCTCGACCGGCTGGCGCAGCGGCGTGAGCGC
GCCGACGGTGTCGGCCGGCAGGTGGCAGCGACCCGCTGA

Upstream 100 bases:

>100_bases
GCCGGACCACGACCTTGCGGCCGTCCGGCAGCCTCCTCGCCCATCACGCGCCGGCCGCCGCCGCTCCGATTTTCGATACC
CGAACACGAAGGAAACGACC

Downstream 100 bases:

>100_bases
TGTGACTGCGGCCCCGTGAGGGGCCGCAGCATTATTCCGGGCGGGATGTTCCCGGCGGGTGGTGCCGGGAAAGGCTTCAG
CGATTGCCGCCCGGCTTGGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MLKNPHTKYRPANPFAGVVPGGRGLSDRTWPDNTITRPPVWMSTDLRDGNQALFEPMNSERKMRMFKMLVEIGLKEIEVA
FPAASQTDFDFVRELIEGGHIPDDVTIEVLTQARPHLIERTFESVRGAKRAIVHVYNAVAPNFRRIVFDTDRAGVKQIAV
DSARYFVEMAARQPGTDFTFQYSPEVFSGTELDFAVEVANAVIEVWQPTPQQKCIINLPATVEMSTPNVYADQIEWMHRH
LARRDSVLLSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYSQGVHPGLDFSRINEVARTVE
HCTQLPIHPRHPYVGDLVFTAFSGSHQDAIKKGFAVQQPDAVWEVPYLPVDPADLGRSYESIIRVNSQSGKGGIAYLLEA
EYGLVMPRRLQVEFSAAIQRITDERGTELSAGDIWRAFEEEYLAVAAPYAYVEHHLAEHGGQQGISLTVEEAGVRRVLRG
VGNGPIDAALHALDAGAVLLGYEERAIGQGGDARAVAYIELADGEGGGSTFGVGIHANIVTASVLAIVSALDRLAQRRER
ADGVGRQVAATR

Sequences:

>Translated_572_residues
MLKNPHTKYRPANPFAGVVPGGRGLSDRTWPDNTITRPPVWMSTDLRDGNQALFEPMNSERKMRMFKMLVEIGLKEIEVA
FPAASQTDFDFVRELIEGGHIPDDVTIEVLTQARPHLIERTFESVRGAKRAIVHVYNAVAPNFRRIVFDTDRAGVKQIAV
DSARYFVEMAARQPGTDFTFQYSPEVFSGTELDFAVEVANAVIEVWQPTPQQKCIINLPATVEMSTPNVYADQIEWMHRH
LARRDSVLLSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYSQGVHPGLDFSRINEVARTVE
HCTQLPIHPRHPYVGDLVFTAFSGSHQDAIKKGFAVQQPDAVWEVPYLPVDPADLGRSYESIIRVNSQSGKGGIAYLLEA
EYGLVMPRRLQVEFSAAIQRITDERGTELSAGDIWRAFEEEYLAVAAPYAYVEHHLAEHGGQQGISLTVEEAGVRRVLRG
VGNGPIDAALHALDAGAVLLGYEERAIGQGGDARAVAYIELADGEGGGSTFGVGIHANIVTASVLAIVSALDRLAQRRER
ADGVGRQVAATR
>Mature_572_residues
MLKNPHTKYRPANPFAGVVPGGRGLSDRTWPDNTITRPPVWMSTDLRDGNQALFEPMNSERKMRMFKMLVEIGLKEIEVA
FPAASQTDFDFVRELIEGGHIPDDVTIEVLTQARPHLIERTFESVRGAKRAIVHVYNAVAPNFRRIVFDTDRAGVKQIAV
DSARYFVEMAARQPGTDFTFQYSPEVFSGTELDFAVEVANAVIEVWQPTPQQKCIINLPATVEMSTPNVYADQIEWMHRH
LARRDSVLLSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYSQGVHPGLDFSRINEVARTVE
HCTQLPIHPRHPYVGDLVFTAFSGSHQDAIKKGFAVQQPDAVWEVPYLPVDPADLGRSYESIIRVNSQSGKGGIAYLLEA
EYGLVMPRRLQVEFSAAIQRITDERGTELSAGDIWRAFEEEYLAVAAPYAYVEHHLAEHGGQQGISLTVEEAGVRRVLRG
VGNGPIDAALHALDAGAVLLGYEERAIGQGGDARAVAYIELADGEGGGSTFGVGIHANIVTASVLAIVSALDRLAQRRER
ADGVGRQVAATR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=542, Percent_Identity=25.2767527675277, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6324225, Length=575, Percent_Identity=47.1304347826087, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6324682, Length=575, Percent_Identity=45.0434782608696, Blast_Score=505, Evalue=1e-144,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_AZOSB (A1KBD3)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_935025.1
- ProteinModelPortal:   A1KBD3
- SMR:   A1KBD3
- STRING:   A1KBD3
- GeneID:   4609718
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3523
- eggNOG:   COG0119
- HOGENOM:   HBG289752
- OMA:   SASQTEF
- PhylomeDB:   A1KBD3
- ProtClustDB:   PRK03739
- BioCyc:   ASP62928:AZO3523-MONOMER
- HAMAP:   MF_00572
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR10277:SF2
- SMART:   SM00917
- TIGRFAMs:   TIGR00970

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 62630; Mature: 62630

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNPHTKYRPANPFAGVVPGGRGLSDRTWPDNTITRPPVWMSTDLRDGNQALFEPMNSE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHCCCCCH
RKMRMFKMLVEIGLKEIEVAFPAASQTDFDFVRELIEGGHIPDDVTIEVLTQARPHLIER
HHHHHHHHHHHHCHHHEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHH
TFESVRGAKRAIVHVYNAVAPNFRRIVFDTDRAGVKQIAVDSARYFVEMAARQPGTDFTF
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEE
QYSPEVFSGTELDFAVEVANAVIEVWQPTPQQKCIINLPATVEMSTPNVYADQIEWMHRH
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEECCCCHHHHHHHHHHHH
LARRDSVLLSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYS
HHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCHHCEEEECCCCCCCCEEEEEEEEHHHH
QGVHPGLDFSRINEVARTVEHCTQLPIHPRHPYVGDLVFTAFSGSHQDAIKKGFAVQQPD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCCHHHHHCCCCCCCCC
AVWEVPYLPVDPADLGRSYESIIRVNSQSGKGGIAYLLEAEYGLVMPRRLQVEFSAAIQR
CEEECCCCCCCHHHHCCCHHHHEEECCCCCCCCEEEEEECCCCEECCCEEEEHHHHHHHH
ITDERGTELSAGDIWRAFEEEYLAVAAPYAYVEHHLAEHGGQQGISLTVEEAGVRRVLRG
HHHHCCCCCCHHHHHHHHHHHHHEEECHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
VGNGPIDAALHALDAGAVLLGYEERAIGQGGDARAVAYIELADGEGGGSTFGVGIHANIV
CCCCCHHHHHHHHCCCEEEEECHHHHCCCCCCCEEEEEEEEECCCCCCCEEEEEEEHHHH
TASVLAIVSALDRLAQRRERADGVGRQVAATR
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MLKNPHTKYRPANPFAGVVPGGRGLSDRTWPDNTITRPPVWMSTDLRDGNQALFEPMNSE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHCCCCCH
RKMRMFKMLVEIGLKEIEVAFPAASQTDFDFVRELIEGGHIPDDVTIEVLTQARPHLIER
HHHHHHHHHHHHCHHHEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHH
TFESVRGAKRAIVHVYNAVAPNFRRIVFDTDRAGVKQIAVDSARYFVEMAARQPGTDFTF
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEE
QYSPEVFSGTELDFAVEVANAVIEVWQPTPQQKCIINLPATVEMSTPNVYADQIEWMHRH
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEECCCCHHHHHHHHHHHH
LARRDSVLLSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYS
HHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCHHCEEEECCCCCCCCEEEEEEEEHHHH
QGVHPGLDFSRINEVARTVEHCTQLPIHPRHPYVGDLVFTAFSGSHQDAIKKGFAVQQPD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCCHHHHHCCCCCCCCC
AVWEVPYLPVDPADLGRSYESIIRVNSQSGKGGIAYLLEAEYGLVMPRRLQVEFSAAIQR
CEEECCCCCCCHHHHCCCHHHHEEECCCCCCCCEEEEEECCCCEECCCEEEEHHHHHHHH
ITDERGTELSAGDIWRAFEEEYLAVAAPYAYVEHHLAEHGGQQGISLTVEEAGVRRVLRG
HHHHCCCCCCHHHHHHHHHHHHHEEECHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
VGNGPIDAALHALDAGAVLLGYEERAIGQGGDARAVAYIELADGEGGGSTFGVGIHANIV
CCCCCHHHHHHHHCCCEEEEECHHHHCCCCCCCEEEEEEEEECCCCCCCEEEEEEEHHHH
TASVLAIVSALDRLAQRRERADGVGRQVAATR
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA