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The map label for this gene is cobQ

Identifier: 119899806

GI number: 119899806

Start: 3858601

End: 3860079

Strand: Reverse

Name: cobQ

Synonym: azo3517

Alternate gene names: 119899806

Gene position: 3860079-3858601 (Counterclockwise)

Preceding gene: 119899810

Following gene: 119899804

Centisome position: 88.21

GC content: 71.06

Gene sequence:

>1479_bases
ATGTCCAAGGCTTCCGCCCTGATGGTGCAGGGCACCACCTCCGATGCCGGCAAGAGCACCCTGGTGGCCGGGCTGGCGCG
CGCGCTGGTGCGCCGCGGCGTGCGCGTCGCCCCTTTCAAGCCGCAGAACATGGCGCTGAATTCGGCGGTCACCGCCGACG
GCGGCGAGATCGGGCGCGCCCAGGCGCTGCAGGCGGTGGCCGCACGCATCGCGCCGCACACCGACTTCAACCCGGTGCTG
CTCAAGCCCTCGTCCGACATCGGCGCCCAGGTCATCATCCACGGCAAGGTCATGGCCAATCTGTCGGCGCGCGATTACCA
CGCCTACAAGCCGACCGCGATGGCGGCGGTGATGGCGTCCTTCGACCGCCTTTCCGCGCAGTACGAGCACGTGCTGATCG
AAGGCGCCGGCAGCCCGGCCGAGATCAACCTGCGCGACCGCGACATCGCCAACATGGGCTTTGCCGAAGCCGCCGACGTG
CCGGTGGTGCTGGTCGCCGACATCGACCGCGGCGGCGTGTTTGCCCACCTGGTCGGCACCCTGGAACTGCTGTCACCGTC
CGAGCAGGCGCGGGTCAGGGGCTTCGTGATCAATCGCTTCCGCGGCGACATCGCGCTGCTGCAGCCGGGGCTGGACTGGC
TGACCGAGCGCACCGGGCGGCCGGTGTTCGGCGTGCTGCCCTATCTCCATGGCCTCTTCCTCGACGCCGAGGATGCGCTG
GCCAACGGCCAGGTCGCAGCCGACCGCGATGGCGCGGTGCTGAAGGTGATCGCGCCGGTCTATCCGCGCATCTCCAACCA
CACCGACCTCGACGCGCTGCGCCTGCATCCGCAGGTGGATTTCCGCTGGGTGGGGCCGGGGCAGGCGATTCCGCCGGCCG
ATCTGGTAGTGCTGCCGGGCTCCAAGAGCGTGCAGGCCGACCTCGCCTGGCTGCGCGCGCAGGGCTGGGAGCCGGCGCTG
CGCCGCCACCTGCGCTACGGCGGCAAGGTGATCGGCATCTGCGGTGGCTTCCAGATGCTGGGCAGCACGCTGGCCGATCC
ACATGGCCTCGAAGGCGCGCCGAGCACCGTCGCCGGACTGGGCGCGCTCGACATCGAGACGGTGCTCGAGCGCGAGAAGC
AGCTGGTGAATGTGCGCGGTCGCCTTGCGCTGGATGGCGAGGCGGCGGTTGCCGGCTACGAGATCCACATGGGGGTGAGC
CGGGGCGCCGATCTCGACCGCCCGGCGGTGCTGCTGGAAGATGGCCGTGCCGACGGCGCGCTATCGGCCGACGGCCAGGT
GCTCGGCACCTATCTGCATGGTCTGTTCGACACCCCGCAGGCGGTGGCGGCGCTGCTCGACTGGGCAGGGCTGAAAGGCG
CGGCGGGCGTCGATCTCAACGCCCGCCGCGAGGCCGACCTCGACCGCCTCGCCGATGCGGTCGAGGCGCATGTCGACATG
GCGGCGCTGTTCGGCGCCGCGTGGCCGGACGCCGCTTAG

Upstream 100 bases:

>100_bases
CGGAACCGTGGGGGCGGGAAACAGGGCGGCAGCGGGACGCCGGGCGGCCGGCACTGTGGTGGATAATCACGGCCCCGAAC
AAGGCGACGGATTTTACGCG

Downstream 100 bases:

>100_bases
CCGCGCAGCCGGCGCGCGATCTCGCTGACGCGTACGCCGTAGAGGCGGGCGGTTTCGAGGTCGCCGGCGGAGATCTCGTC
CGCGCTCGCGTCCGACGGGC

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSKASALMVQGTTSDAGKSTLVAGLARALVRRGVRVAPFKPQNMALNSAVTADGGEIGRAQALQAVAARIAPHTDFNPVL
LKPSSDIGAQVIIHGKVMANLSARDYHAYKPTAMAAVMASFDRLSAQYEHVLIEGAGSPAEINLRDRDIANMGFAEAADV
PVVLVADIDRGGVFAHLVGTLELLSPSEQARVRGFVINRFRGDIALLQPGLDWLTERTGRPVFGVLPYLHGLFLDAEDAL
ANGQVAADRDGAVLKVIAPVYPRISNHTDLDALRLHPQVDFRWVGPGQAIPPADLVVLPGSKSVQADLAWLRAQGWEPAL
RRHLRYGGKVIGICGGFQMLGSTLADPHGLEGAPSTVAGLGALDIETVLEREKQLVNVRGRLALDGEAAVAGYEIHMGVS
RGADLDRPAVLLEDGRADGALSADGQVLGTYLHGLFDTPQAVAALLDWAGLKGAAGVDLNARREADLDRLADAVEAHVDM
AALFGAAWPDAA

Sequences:

>Translated_492_residues
MSKASALMVQGTTSDAGKSTLVAGLARALVRRGVRVAPFKPQNMALNSAVTADGGEIGRAQALQAVAARIAPHTDFNPVL
LKPSSDIGAQVIIHGKVMANLSARDYHAYKPTAMAAVMASFDRLSAQYEHVLIEGAGSPAEINLRDRDIANMGFAEAADV
PVVLVADIDRGGVFAHLVGTLELLSPSEQARVRGFVINRFRGDIALLQPGLDWLTERTGRPVFGVLPYLHGLFLDAEDAL
ANGQVAADRDGAVLKVIAPVYPRISNHTDLDALRLHPQVDFRWVGPGQAIPPADLVVLPGSKSVQADLAWLRAQGWEPAL
RRHLRYGGKVIGICGGFQMLGSTLADPHGLEGAPSTVAGLGALDIETVLEREKQLVNVRGRLALDGEAAVAGYEIHMGVS
RGADLDRPAVLLEDGRADGALSADGQVLGTYLHGLFDTPQAVAALLDWAGLKGAAGVDLNARREADLDRLADAVEAHVDM
AALFGAAWPDAA
>Mature_491_residues
SKASALMVQGTTSDAGKSTLVAGLARALVRRGVRVAPFKPQNMALNSAVTADGGEIGRAQALQAVAARIAPHTDFNPVLL
KPSSDIGAQVIIHGKVMANLSARDYHAYKPTAMAAVMASFDRLSAQYEHVLIEGAGSPAEINLRDRDIANMGFAEAADVP
VVLVADIDRGGVFAHLVGTLELLSPSEQARVRGFVINRFRGDIALLQPGLDWLTERTGRPVFGVLPYLHGLFLDAEDALA
NGQVAADRDGAVLKVIAPVYPRISNHTDLDALRLHPQVDFRWVGPGQAIPPADLVVLPGSKSVQADLAWLRAQGWEPALR
RHLRYGGKVIGICGGFQMLGSTLADPHGLEGAPSTVAGLGALDIETVLEREKQLVNVRGRLALDGEAAVAGYEIHMGVSR
GADLDRPAVLLEDGRADGALSADGQVLGTYLHGLFDTPQAVAALLDWAGLKGAAGVDLNARREADLDRLADAVEAHVDMA
ALFGAAWPDAA

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_AZOSB (A1KBC7)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_935019.1
- ProteinModelPortal:   A1KBC7
- STRING:   A1KBC7
- GeneID:   4610114
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3517
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   AHTDLNP
- ProtClustDB:   PRK00784
- BioCyc:   ASP62928:AZO3517-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 51819; Mature: 51688

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 334-334 ACT_SITE 433-433

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKASALMVQGTTSDAGKSTLVAGLARALVRRGVRVAPFKPQNMALNSAVTADGGEIGRA
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEECCEEECCCCCCCHH
QALQAVAARIAPHTDFNPVLLKPSSDIGAQVIIHGKVMANLSARDYHAYKPTAMAAVMAS
HHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
FDRLSAQYEHVLIEGAGSPAEINLRDRDIANMGFAEAADVPVVLVADIDRGGVFAHLVGT
HHHHHHCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
LELLSPSEQARVRGFVINRFRGDIALLQPGLDWLTERTGRPVFGVLPYLHGLFLDAEDAL
HHHCCCCHHHHHHHHEEEHHCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHEEECCHHHH
ANGQVAADRDGAVLKVIAPVYPRISNHTDLDALRLHPQVDFRWVGPGQAIPPADLVVLPG
CCCCEEECCCCCEEEEECCCCCCCCCCCCCHHEEECCCCCEEECCCCCCCCCCCEEEECC
SKSVQADLAWLRAQGWEPALRRHLRYGGKVIGICGGFQMLGSTLADPHGLEGAPSTVAGL
CCCHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHCCCCCCCCCCCHHHCC
GALDIETVLEREKQLVNVRGRLALDGEAAVAGYEIHMGVSRGADLDRPAVLLEDGRADGA
CCHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEEEECCCCCCCCCCCEEEEECCCCCCC
LSADGQVLGTYLHGLFDTPQAVAALLDWAGLKGAAGVDLNARREADLDRLADAVEAHVDM
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AALFGAAWPDAA
HHHHCCCCCCCC
>Mature Secondary Structure 
SKASALMVQGTTSDAGKSTLVAGLARALVRRGVRVAPFKPQNMALNSAVTADGGEIGRA
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEECCEEECCCCCCCHH
QALQAVAARIAPHTDFNPVLLKPSSDIGAQVIIHGKVMANLSARDYHAYKPTAMAAVMAS
HHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
FDRLSAQYEHVLIEGAGSPAEINLRDRDIANMGFAEAADVPVVLVADIDRGGVFAHLVGT
HHHHHHCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
LELLSPSEQARVRGFVINRFRGDIALLQPGLDWLTERTGRPVFGVLPYLHGLFLDAEDAL
HHHCCCCHHHHHHHHEEEHHCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHEEECCHHHH
ANGQVAADRDGAVLKVIAPVYPRISNHTDLDALRLHPQVDFRWVGPGQAIPPADLVVLPG
CCCCEEECCCCCEEEEECCCCCCCCCCCCCHHEEECCCCCEEECCCCCCCCCCCEEEECC
SKSVQADLAWLRAQGWEPALRRHLRYGGKVIGICGGFQMLGSTLADPHGLEGAPSTVAGL
CCCHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHCCCCCCCCCCCHHHCC
GALDIETVLEREKQLVNVRGRLALDGEAAVAGYEIHMGVSRGADLDRPAVLLEDGRADGA
CCHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEEEECCCCCCCCCCCEEEEECCCCCCC
LSADGQVLGTYLHGLFDTPQAVAALLDWAGLKGAAGVDLNARREADLDRLADAVEAHVDM
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AALFGAAWPDAA
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA