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The map label for this gene is pilJ [H]

Identifier: 119899745

GI number: 119899745

Start: 3788002

End: 3790122

Strand: Direct

Name: pilJ [H]

Synonym: azo3456

Alternate gene names: 119899745

Gene position: 3788002-3790122 (Clockwise)

Preceding gene: 119899744

Following gene: 119899746

Centisome position: 86.56

GC content: 66.71

Gene sequence:

>2121_bases
ATGGCTTTCAAGCTCACCTTCGGCAAGAAAATGCTGGCTGCGGACGACCTCGTCTCCGACACCACCATCATGGAAGCGCC
TGGCACCCCTCCGCGGAAAGGCAAAGCGGCCAAGCCGCGCTCCAACCGCCCGGTCGGTGACCAGATCAAGGTTCTCGGCG
CCCTCTTCGCCGGACTCGTTGCCGTCACCGCCGGCCTCGTCATCTATCAGGGCCGCCAGTCGGCACACGCCACCACCTAC
GTGGCGGCAGCCGGCGAAATGCAGATGCTGTCGCAGCAGATCGCCAAGTCGGCGCAGCTTGCGCTGCAAGGCAGCCCGGC
GTCCTTCGCCGAACTGCGCAGCGGTAGCGCGCGCTTCACCAGCCTGCTGCAGGCGCTCGACCAGGGCGGCGACATCGACG
GCGTGGGCGTGCCGTCCATTCCCGACAGCAGCCGCGCCCAGCTCGATGCGCTGGCCGAAACCTGGAGCAAGTCGGCCAAG
GACACGGAACAACTGCTGGCCCAGGAAAAGAACCTGATCACCCTCGACGCGGCGGTCGACGCGATCAATGCCGGCAACGC
CAACCTGCTCGACCTCACCGAGCAGATTGCCTCCCTCAAGCTCCAGGCCGGTGCGCCGGCGCGCGAGATCGCCACCGCCA
ACCACGCGGTGATGCTGACCCAGCGCATCGCCAAGAACGCCAATGCGCTGCTGGTCGCCGACGCCATCGACCCGGAAACC
GCGTTCGTGCTCGGCAAGGACACCAACACCTTCCGCGAGCAGATCGAACTGCTCGGCCGCATGTCCGGCGACGCCGACCT
GCGCAACCGCGTCGCCGCGCTGGAGGAAGCGGCCCAGGGGACGATGACCGCCGTCACCAACATGCTGGGCAACATCCAGC
TGCTGGTGCAGGCCAAGCAGGCCGGCGCGCGCATCTTCCGCGACGCCACGCCCTTGCTCGCCCGCACCGGCGAACTTGCC
GACGCGCTCAACAGCAGTTCGCGCGGCTTCAACCCGTTGTCCGCGCTGATCGCCCTGACTGCGCTCGCCGGACTGGGCGT
GCTGGCGAAAATGGCCAAGATCTACAGCGACGACCTTGCCTCGCGCCGTACCGAATCCGAAACCCAGCGCCAGGCCGCCG
AGAACGAACGCAACCTCACCCAGCAGGCCATCCTGCGGCTGATGAACGAGATGGGCGACCTCGCGGACGGTGACCTCACG
ATCCGCGCCACGGTGACCGAAGACATCACCGGCGCGATCGCCGACTCGGTGAACTACACCATCGAAGAGCTCTCGGTGCT
GGTCCGCCGCATCAACGACGCCGCGGTGCGGGTGACCGCGGCCACGCAGTCCGCGCAGAAGACCTCGAACGAACTGCTCG
GCGCCACCGAGCGCCAGACCCGCGAAATCGAGGAAGCCGGCGACACCGTCGAGCGGATGGCGCAGTCGATGGCGGAATCC
TCCGAGCGCGCCCTGCAGTCCGCGCAGGTCGCGCGTCGCTCGCTCGATTCGGCGCGCAAGGGGGCGGATGCGGTGGAGAA
CACCATCAAGGGCATGAACGAGATCCGCGAGAAGATCCAGGAGACCTCCAAGCGCATCAAGCGCCTCGGCGAATCGTCGC
AGGAGATCGGTGAAATCGTGGAACTGATCTCGGACATTACCGAGCAGACCAACGTGCTCGCGCTCAACGCCGCCATCCAG
GCTGCCTCGGCGGGCGAAGCCGGCCGCGGCTTCACGGTGGTTGCGGAAGAAGTGCAGCGGCTGGCGGAGCGTTCCGCCGA
AGCAACCAAGCAGATCGCGGCGATCGTGAAGACGATTCAGACCGACACCCAGGATGCCGTCGGCGCAATGGAAAACGCCA
CCCGCGACGTGGTCGAGGGCGCCCAGCTGTCCGATGCCGCAGGCAAGGCGCTGGCCGAGATCGGCGAGGTGTCGATGGAA
ACCGCCCGCCTCATCGAGCAGATTTCGAACGATACGCAGCAGCAGGCTTCCACCGCCGTGCGCGTGGCCGGCACGATGAA
GGACATTCTCGCGATTACCGAACAGACCACGCTCGGCACCAAGCAGACCGCGGTCTCCATCGGCCAGCTCGCCGACCTCG
CGGTCGAACTGAAGGGTTCCGTTTCCGGCTTCAAGGTCTGA

Upstream 100 bases:

>100_bases
GCCGGCACTGCTCGCCCATCCGGGCTTCCTCGACGCCGGGCTCGACTGAGCGCCGGCCGGACTCCAAACACTCACATCCA
TTTGCGGCGGAGCACGCGTT

Downstream 100 bases:

>100_bases
AACCACCGTTGCACCAGCAGTTAGGAAAGCCGCCATGACGCACGCCACCGAAGCGGACCTCGGTCCGCTCACCTGGGTCA
AGAGCGAGATCGACGCGGCC

Product: pilus biogenesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 706; Mature: 705

Protein sequence:

>706_residues
MAFKLTFGKKMLAADDLVSDTTIMEAPGTPPRKGKAAKPRSNRPVGDQIKVLGALFAGLVAVTAGLVIYQGRQSAHATTY
VAAAGEMQMLSQQIAKSAQLALQGSPASFAELRSGSARFTSLLQALDQGGDIDGVGVPSIPDSSRAQLDALAETWSKSAK
DTEQLLAQEKNLITLDAAVDAINAGNANLLDLTEQIASLKLQAGAPAREIATANHAVMLTQRIAKNANALLVADAIDPET
AFVLGKDTNTFREQIELLGRMSGDADLRNRVAALEEAAQGTMTAVTNMLGNIQLLVQAKQAGARIFRDATPLLARTGELA
DALNSSSRGFNPLSALIALTALAGLGVLAKMAKIYSDDLASRRTESETQRQAAENERNLTQQAILRLMNEMGDLADGDLT
IRATVTEDITGAIADSVNYTIEELSVLVRRINDAAVRVTAATQSAQKTSNELLGATERQTREIEEAGDTVERMAQSMAES
SERALQSAQVARRSLDSARKGADAVENTIKGMNEIREKIQETSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQ
AASAGEAGRGFTVVAEEVQRLAERSAEATKQIAAIVKTIQTDTQDAVGAMENATRDVVEGAQLSDAAGKALAEIGEVSME
TARLIEQISNDTQQQASTAVRVAGTMKDILAITEQTTLGTKQTAVSIGQLADLAVELKGSVSGFKV

Sequences:

>Translated_706_residues
MAFKLTFGKKMLAADDLVSDTTIMEAPGTPPRKGKAAKPRSNRPVGDQIKVLGALFAGLVAVTAGLVIYQGRQSAHATTY
VAAAGEMQMLSQQIAKSAQLALQGSPASFAELRSGSARFTSLLQALDQGGDIDGVGVPSIPDSSRAQLDALAETWSKSAK
DTEQLLAQEKNLITLDAAVDAINAGNANLLDLTEQIASLKLQAGAPAREIATANHAVMLTQRIAKNANALLVADAIDPET
AFVLGKDTNTFREQIELLGRMSGDADLRNRVAALEEAAQGTMTAVTNMLGNIQLLVQAKQAGARIFRDATPLLARTGELA
DALNSSSRGFNPLSALIALTALAGLGVLAKMAKIYSDDLASRRTESETQRQAAENERNLTQQAILRLMNEMGDLADGDLT
IRATVTEDITGAIADSVNYTIEELSVLVRRINDAAVRVTAATQSAQKTSNELLGATERQTREIEEAGDTVERMAQSMAES
SERALQSAQVARRSLDSARKGADAVENTIKGMNEIREKIQETSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQ
AASAGEAGRGFTVVAEEVQRLAERSAEATKQIAAIVKTIQTDTQDAVGAMENATRDVVEGAQLSDAAGKALAEIGEVSME
TARLIEQISNDTQQQASTAVRVAGTMKDILAITEQTTLGTKQTAVSIGQLADLAVELKGSVSGFKV
>Mature_705_residues
AFKLTFGKKMLAADDLVSDTTIMEAPGTPPRKGKAAKPRSNRPVGDQIKVLGALFAGLVAVTAGLVIYQGRQSAHATTYV
AAAGEMQMLSQQIAKSAQLALQGSPASFAELRSGSARFTSLLQALDQGGDIDGVGVPSIPDSSRAQLDALAETWSKSAKD
TEQLLAQEKNLITLDAAVDAINAGNANLLDLTEQIASLKLQAGAPAREIATANHAVMLTQRIAKNANALLVADAIDPETA
FVLGKDTNTFREQIELLGRMSGDADLRNRVAALEEAAQGTMTAVTNMLGNIQLLVQAKQAGARIFRDATPLLARTGELAD
ALNSSSRGFNPLSALIALTALAGLGVLAKMAKIYSDDLASRRTESETQRQAAENERNLTQQAILRLMNEMGDLADGDLTI
RATVTEDITGAIADSVNYTIEELSVLVRRINDAAVRVTAATQSAQKTSNELLGATERQTREIEEAGDTVERMAQSMAESS
ERALQSAQVARRSLDSARKGADAVENTIKGMNEIREKIQETSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQA
ASAGEAGRGFTVVAEEVQRLAERSAEATKQIAAIVKTIQTDTQDAVGAMENATRDVVEGAQLSDAAGKALAEIGEVSMET
ARLIEQISNDTQQQASTAVRVAGTMKDILAITEQTTLGTKQTAVSIGQLADLAVELKGSVSGFKV

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=306, Percent_Identity=28.7581699346405, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788195, Length=265, Percent_Identity=29.4339622641509, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789453, Length=241, Percent_Identity=32.3651452282158, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788194, Length=256, Percent_Identity=29.6875, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1787690, Length=314, Percent_Identity=25.4777070063694, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 74563; Mature: 74432

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFKLTFGKKMLAADDLVSDTTIMEAPGTPPRKGKAAKPRSNRPVGDQIKVLGALFAGLV
CEEEEECCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVTAGLVIYQGRQSAHATTYVAAAGEMQMLSQQIAKSAQLALQGSPASFAELRSGSARFT
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHH
SLLQALDQGGDIDGVGVPSIPDSSRAQLDALAETWSKSAKDTEQLLAQEKNLITLDAAVD
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEHHHHH
AINAGNANLLDLTEQIASLKLQAGAPAREIATANHAVMLTQRIAKNANALLVADAIDPET
HHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCCC
AFVLGKDTNTFREQIELLGRMSGDADLRNRVAALEEAAQGTMTAVTNMLGNIQLLVQAKQ
EEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHHH
AGARIFRDATPLLARTGELADALNSSSRGFNPLSALIALTALAGLGVLAKMAKIYSDDLA
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRTESETQRQAAENERNLTQQAILRLMNEMGDLADGDLTIRATVTEDITGAIADSVNYT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHH
IEELSVLVRRINDAAVRVTAATQSAQKTSNELLGATERQTREIEEAGDTVERMAQSMAES
HHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SERALQSAQVARRSLDSARKGADAVENTIKGMNEIREKIQETSKRIKRLGESSQEIGEIV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ELISDITEQTNVLALNAAIQAASAGEAGRGFTVVAEEVQRLAERSAEATKQIAAIVKTIQ
HHHHHHHHHCCEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDTQDAVGAMENATRDVVEGAQLSDAAGKALAEIGEVSMETARLIEQISNDTQQQASTAV
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
RVAGTMKDILAITEQTTLGTKQTAVSIGQLADLAVELKGSVSGFKV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AFKLTFGKKMLAADDLVSDTTIMEAPGTPPRKGKAAKPRSNRPVGDQIKVLGALFAGLV
EEEEECCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVTAGLVIYQGRQSAHATTYVAAAGEMQMLSQQIAKSAQLALQGSPASFAELRSGSARFT
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHH
SLLQALDQGGDIDGVGVPSIPDSSRAQLDALAETWSKSAKDTEQLLAQEKNLITLDAAVD
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEHHHHH
AINAGNANLLDLTEQIASLKLQAGAPAREIATANHAVMLTQRIAKNANALLVADAIDPET
HHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCCC
AFVLGKDTNTFREQIELLGRMSGDADLRNRVAALEEAAQGTMTAVTNMLGNIQLLVQAKQ
EEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHHH
AGARIFRDATPLLARTGELADALNSSSRGFNPLSALIALTALAGLGVLAKMAKIYSDDLA
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRTESETQRQAAENERNLTQQAILRLMNEMGDLADGDLTIRATVTEDITGAIADSVNYT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHH
IEELSVLVRRINDAAVRVTAATQSAQKTSNELLGATERQTREIEEAGDTVERMAQSMAES
HHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SERALQSAQVARRSLDSARKGADAVENTIKGMNEIREKIQETSKRIKRLGESSQEIGEIV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ELISDITEQTNVLALNAAIQAASAGEAGRGFTVVAEEVQRLAERSAEATKQIAAIVKTIQ
HHHHHHHHHCCEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDTQDAVGAMENATRDVVEGAQLSDAAGKALAEIGEVSMETARLIEQISNDTQQQASTAV
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
RVAGTMKDILAITEQTTLGTKQTAVSIGQLADLAVELKGSVSGFKV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]