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The map label for this gene is hemL

Identifier: 119899738

GI number: 119899738

Start: 3783478

End: 3784752

Strand: Reverse

Name: hemL

Synonym: azo3449

Alternate gene names: 119899738

Gene position: 3784752-3783478 (Counterclockwise)

Preceding gene: 119899739

Following gene: 119899736

Centisome position: 86.49

GC content: 69.25

Gene sequence:

>1275_bases
ATGACCAGTCGCAATGAAGCCCTTTTCAGCCGCGCCCAGCGCAGCATTCCCGGCGGGGTGAATTCCCCCGTGCGCGCCTT
CCGCTCCGTTGGCGGCACGCCGCGCTTCATCGAGCGCGCGGAAGGGGCGAGGGTCTGGGATGCGGATGGCAAGGCCTACA
TCGACTACGTGGGGTCCTGGGGTCCGGCGATCGCCGGTCACTCGCACCCGGCGATCGTCGAGGCGGTGCGCGAGGCGGCG
CTGCGCGGGTTGTCGTTCGGGGCGCCGACCGAGAGCGAGGTCGAGATGGCCGAACTGCTGTGCGCGCTGCTGCCGTCGAT
CGAGATGTTGCGGCTGGTGAGTTCGGGCACCGAGGCCACGATGAGCGCGATCCGGCTCGCCCGCGGCTTCACCGGGCGTG
ACGACATCATCAAGTTCGAGGGCTGCTATCACGGCCACGCCGACAGCCTGCTGGTGAAGGCCGGGTCGGGCCTGCTGACC
TTCGGCAATCCGTCGTCCGGCGGCGTGCCGGCCGACTTCGCCCACCATACCGTGGTGCTCGACTACAACGATCTCGATCA
GGTCGAGACGGTGTTCAAGGCGCGTGGCGATCAGATTGCCGCGGTCATCGTGGAGCCGGTGGCGGGCAACATGAACCTGA
TAAAGCCGCGCCCGGGCTTTCTCGAAGGGCTGCGCCGCTTGTGTACCGAGTACGGCGCGGTGCTGATCTTCGACGAGGTG
ATGACCGGCTTTCGCGTCGGCCCTCAGGGGGTGCAGGGGCTGTACGGCATCACGCCCGATCTGACCACGCTGGGCAAGGT
GATCGGCGGCGGCATGCCGGTGGGGGCGTTCGGCGGGCGGCGCGACATCATGAACTGCATTGCGCCGCTCGGGCCGGTGT
ACCAGGCGGGCACGCTGTCAGGCAGCCCCGTGGCGGTGGCGGCGGGCCTGGCCTCGCTGCGCCTGACCCAGGCGCCCGGC
TTCTACGATGCGCTGGCGGCCCGCGCGCGGTCGCTGGTGGATGGTCTGTCAGGCGTGGCGCGCGAGGCTGGCGTGCCGTT
CAGCGCGGATTCGATCGGCGGCATGTTCGGCATCTACTTCAGCCCCTCGGTGCCTGCGTCGTTTGCGGAGGTGATGGCCT
CGGATCGCGAGGCCTTCAACCGCTTCTTCCACGCCATGCTCGACGCCGGCCATTACTTCGCCCCGTCGGCGTTCGAGGCG
GGTTTCGTGTCGGCCGCGCATGGCGAGGCCGACATTCAGGCTACCGTGGCTGCCGCGCGCGCCTGCCTGCGCTGA

Upstream 100 bases:

>100_bases
CGCCTATTCGGCCCTGTTCGCCTGAAACTGCCCGCGGTGGACCGGCGCCGGTGGGCGCGCGACAATCCCGCCCTTTCCAT
AGCAGTCGCGAGTCGCAGCC

Downstream 100 bases:

>100_bases
GCGCTTGCGTCAGCGCTTGCCGCGGCGGCGCAGGGTGAAGCCCAGCAGGCCGACGGCGGCAAGGGCGAGGCCGCCGGCGG
GCGGTTCGGGGACGACCTGA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTSRNEALFSRAQRSIPGGVNSPVRAFRSVGGTPRFIERAEGARVWDADGKAYIDYVGSWGPAIAGHSHPAIVEAVREAA
LRGLSFGAPTESEVEMAELLCALLPSIEMLRLVSSGTEATMSAIRLARGFTGRDDIIKFEGCYHGHADSLLVKAGSGLLT
FGNPSSGGVPADFAHHTVVLDYNDLDQVETVFKARGDQIAAVIVEPVAGNMNLIKPRPGFLEGLRRLCTEYGAVLIFDEV
MTGFRVGPQGVQGLYGITPDLTTLGKVIGGGMPVGAFGGRRDIMNCIAPLGPVYQAGTLSGSPVAVAAGLASLRLTQAPG
FYDALAARARSLVDGLSGVAREAGVPFSADSIGGMFGIYFSPSVPASFAEVMASDREAFNRFFHAMLDAGHYFAPSAFEA
GFVSAAHGEADIQATVAAARACLR

Sequences:

>Translated_424_residues
MTSRNEALFSRAQRSIPGGVNSPVRAFRSVGGTPRFIERAEGARVWDADGKAYIDYVGSWGPAIAGHSHPAIVEAVREAA
LRGLSFGAPTESEVEMAELLCALLPSIEMLRLVSSGTEATMSAIRLARGFTGRDDIIKFEGCYHGHADSLLVKAGSGLLT
FGNPSSGGVPADFAHHTVVLDYNDLDQVETVFKARGDQIAAVIVEPVAGNMNLIKPRPGFLEGLRRLCTEYGAVLIFDEV
MTGFRVGPQGVQGLYGITPDLTTLGKVIGGGMPVGAFGGRRDIMNCIAPLGPVYQAGTLSGSPVAVAAGLASLRLTQAPG
FYDALAARARSLVDGLSGVAREAGVPFSADSIGGMFGIYFSPSVPASFAEVMASDREAFNRFFHAMLDAGHYFAPSAFEA
GFVSAAHGEADIQATVAAARACLR
>Mature_423_residues
TSRNEALFSRAQRSIPGGVNSPVRAFRSVGGTPRFIERAEGARVWDADGKAYIDYVGSWGPAIAGHSHPAIVEAVREAAL
RGLSFGAPTESEVEMAELLCALLPSIEMLRLVSSGTEATMSAIRLARGFTGRDDIIKFEGCYHGHADSLLVKAGSGLLTF
GNPSSGGVPADFAHHTVVLDYNDLDQVETVFKARGDQIAAVIVEPVAGNMNLIKPRPGFLEGLRRLCTEYGAVLIFDEVM
TGFRVGPQGVQGLYGITPDLTTLGKVIGGGMPVGAFGGRRDIMNCIAPLGPVYQAGTLSGSPVAVAAGLASLRLTQAPGF
YDALAARARSLVDGLSGVAREAGVPFSADSIGGMFGIYFSPSVPASFAEVMASDREAFNRFFHAMLDAGHYFAPSAFEAG
FVSAAHGEADIQATVAAARACLR

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=292, Percent_Identity=29.7945205479452, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI24119277, Length=308, Percent_Identity=28.8961038961039, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI37574042, Length=307, Percent_Identity=29.9674267100977, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI226442705, Length=306, Percent_Identity=28.4313725490196, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI226442709, Length=275, Percent_Identity=30.1818181818182, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI284507298, Length=209, Percent_Identity=30.1435406698565, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786349, Length=419, Percent_Identity=63.7231503579952, Blast_Score=543, Evalue=1e-156,
Organism=Escherichia coli, GI1789016, Length=375, Percent_Identity=32, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1789759, Length=334, Percent_Identity=30.2395209580838, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI1788044, Length=332, Percent_Identity=30.7228915662651, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1787560, Length=301, Percent_Identity=34.8837209302326, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI145693181, Length=312, Percent_Identity=30.4487179487179, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1786991, Length=290, Percent_Identity=28.6206896551724, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25144271, Length=347, Percent_Identity=27.0893371757925, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32564660, Length=322, Percent_Identity=25.4658385093168, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25144274, Length=163, Percent_Identity=30.6748466257669, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=348, Percent_Identity=27.2988505747126, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324432, Length=363, Percent_Identity=23.4159779614325, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357415, Length=291, Percent_Identity=29.2096219931272, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356575, Length=336, Percent_Identity=27.9761904761905, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_AZOSB (A1KB59)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_934951.1
- ProteinModelPortal:   A1KB59
- SMR:   A1KB59
- STRING:   A1KB59
- GeneID:   4607956
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3449
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   HANEKIV
- PhylomeDB:   A1KB59
- ProtClustDB:   PRK00062
- BioCyc:   ASP62928:AZO3449-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 44404; Mature: 44273

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRNEALFSRAQRSIPGGVNSPVRAFRSVGGTPRFIERAEGARVWDADGKAYIDYVGSW
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHCCCCEEECCCCCEEEEECCCC
GPAIAGHSHPAIVEAVREAALRGLSFGAPTESEVEMAELLCALLPSIEMLRLVSSGTEAT
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCHHHH
MSAIRLARGFTGRDDIIKFEGCYHGHADSLLVKAGSGLLTFGNPSSGGVPADFAHHTVVL
HHHHHHHHCCCCCCCCEEECCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCCEEEE
DYNDLDQVETVFKARGDQIAAVIVEPVAGNMNLIKPRPGFLEGLRRLCTEYGAVLIFDEV
ECCCHHHHHHHHHHCCCEEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHCCEEEEHHH
MTGFRVGPQGVQGLYGITPDLTTLGKVIGGGMPVGAFGGRRDIMNCIAPLGPVYQAGTLS
HHHCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHCCCCCC
GSPVAVAAGLASLRLTQAPGFYDALAARARSLVDGLSGVAREAGVPFSADSIGGMFGIYF
CCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
SPSVPASFAEVMASDREAFNRFFHAMLDAGHYFAPSAFEAGFVSAAHGEADIQATVAAAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ACLR
HHCC
>Mature Secondary Structure 
TSRNEALFSRAQRSIPGGVNSPVRAFRSVGGTPRFIERAEGARVWDADGKAYIDYVGSW
CCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHCCCCEEECCCCCEEEEECCCC
GPAIAGHSHPAIVEAVREAALRGLSFGAPTESEVEMAELLCALLPSIEMLRLVSSGTEAT
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCHHHH
MSAIRLARGFTGRDDIIKFEGCYHGHADSLLVKAGSGLLTFGNPSSGGVPADFAHHTVVL
HHHHHHHHCCCCCCCCEEECCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCCEEEE
DYNDLDQVETVFKARGDQIAAVIVEPVAGNMNLIKPRPGFLEGLRRLCTEYGAVLIFDEV
ECCCHHHHHHHHHHCCCEEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHCCEEEEHHH
MTGFRVGPQGVQGLYGITPDLTTLGKVIGGGMPVGAFGGRRDIMNCIAPLGPVYQAGTLS
HHHCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHCCCCCC
GSPVAVAAGLASLRLTQAPGFYDALAARARSLVDGLSGVAREAGVPFSADSIGGMFGIYF
CCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
SPSVPASFAEVMASDREAFNRFFHAMLDAGHYFAPSAFEAGFVSAAHGEADIQATVAAAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ACLR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA