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The map label for this gene is ampD2 [H]

Identifier: 119899639

GI number: 119899639

Start: 3687461

End: 3688036

Strand: Reverse

Name: ampD2 [H]

Synonym: azo3350

Alternate gene names: 119899639

Gene position: 3688036-3687461 (Counterclockwise)

Preceding gene: 119899640

Following gene: 119899638

Centisome position: 84.28

GC content: 64.58

Gene sequence:

>576_bases
ATGGCGGGATGGCTGGACGGATGCCGCTGTGTAGCGTCGCCCAATTTCGATCCGCGTCCAGGCGGAGAGCCGCCCAGCCT
GATCATCATCCACGCCATCAGCCTCCCGCCCGACGAGTTTGGCGGTCCCGGCGTCGAGCAACTCTTCACCAATCGGCTCG
ACCCCACCGAGCATCCCTACTACGCGACGATCCACGCCTTGCGCGTCTCCGCGCACTTCTTCATTCGCCGCAACGGCGAG
GTGGTCCAGTTCGTTTCCACCGACGACCGGGCCTGGCATGCCGGGGTTTCGTGTTGGGAGGGGCGGGAGCGGTGCAACGA
TTTTTCCATCGGTATCGAGCTGGAGGGCTGTGACACGCAGCCCTTCGAGGAGGTCCAGTATCGGCAGCTTGCCGGGCTGG
TGACGGCCCTGCGGAGCCGCTATCCGATCGACGCGATTGCGGGGCATTCGGACGTCGCGCCGGGTCGCAAGACCGACCCT
GGCCCCTGCTTCGACTGGGAGCGGCTGCGCGCGCTGCTGGACGGTGGGGTGGGAATGAAGTGGGCGTGTTTTGACAAAGA
TCAAAGGAATCAATAA

Upstream 100 bases:

>100_bases
CGCGGCGGTCACCTGCTGACCGTGAAGCCGGCGGTGTCGCTGGAGACGGTCTTCTTCAAGATACTCGAGGGTGAGGCGCC
CGCGCGCTGACGGAGGCAAC

Downstream 100 bases:

>100_bases
ACTCCGACCCGCTTCAACGATTTGTCGCGTTGCAGCATTGCCTTGAAATCAATTTGCACCAGTGCGACGTTGCTTCGACA
CAGAGGCGGCGCAAGAGTCA

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MAGWLDGCRCVASPNFDPRPGGEPPSLIIIHAISLPPDEFGGPGVEQLFTNRLDPTEHPYYATIHALRVSAHFFIRRNGE
VVQFVSTDDRAWHAGVSCWEGRERCNDFSIGIELEGCDTQPFEEVQYRQLAGLVTALRSRYPIDAIAGHSDVAPGRKTDP
GPCFDWERLRALLDGGVGMKWACFDKDQRNQ

Sequences:

>Translated_191_residues
MAGWLDGCRCVASPNFDPRPGGEPPSLIIIHAISLPPDEFGGPGVEQLFTNRLDPTEHPYYATIHALRVSAHFFIRRNGE
VVQFVSTDDRAWHAGVSCWEGRERCNDFSIGIELEGCDTQPFEEVQYRQLAGLVTALRSRYPIDAIAGHSDVAPGRKTDP
GPCFDWERLRALLDGGVGMKWACFDKDQRNQ
>Mature_190_residues
AGWLDGCRCVASPNFDPRPGGEPPSLIIIHAISLPPDEFGGPGVEQLFTNRLDPTEHPYYATIHALRVSAHFFIRRNGEV
VQFVSTDDRAWHAGVSCWEGRERCNDFSIGIELEGCDTQPFEEVQYRQLAGLVTALRSRYPIDAIAGHSDVAPGRKTDPG
PCFDWERLRALLDGGVGMKWACFDKDQRNQ

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=173, Percent_Identity=59.5375722543353, Blast_Score=211, Evalue=2e-56,
Organism=Escherichia coli, GI1787092, Length=126, Percent_Identity=40.4761904761905, Blast_Score=87, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21299; Mature: 21168

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGWLDGCRCVASPNFDPRPGGEPPSLIIIHAISLPPDEFGGPGVEQLFTNRLDPTEHPY
CCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCHHCCCCCHHHHHHCCCCCCCCCC
YATIHALRVSAHFFIRRNGEVVQFVSTDDRAWHAGVSCWEGRERCNDFSIGIELEGCDTQ
EEEHEEEHEEEEEEEECCCCEEEEEECCCCCEECCCCHHHHHHHCCCEEEEEEEECCCCC
PFEEVQYRQLAGLVTALRSRYPIDAIAGHSDVAPGRKTDPGPCFDWERLRALLDGGVGMK
CHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCE
WACFDKDQRNQ
EEECCCCCCCC
>Mature Secondary Structure 
AGWLDGCRCVASPNFDPRPGGEPPSLIIIHAISLPPDEFGGPGVEQLFTNRLDPTEHPY
CCCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCHHCCCCCHHHHHHCCCCCCCCCC
YATIHALRVSAHFFIRRNGEVVQFVSTDDRAWHAGVSCWEGRERCNDFSIGIELEGCDTQ
EEEHEEEHEEEEEEEECCCCEEEEEECCCCCEECCCCHHHHHHHCCCEEEEEEEECCCCC
PFEEVQYRQLAGLVTALRSRYPIDAIAGHSDVAPGRKTDPGPCFDWERLRALLDGGVGMK
CHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCE
WACFDKDQRNQ
EEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]