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The map label for this gene is asnB2 [H]

Identifier: 119899562

GI number: 119899562

Start: 3609333

End: 3611267

Strand: Reverse

Name: asnB2 [H]

Synonym: azo3273

Alternate gene names: 119899562

Gene position: 3611267-3609333 (Counterclockwise)

Preceding gene: 119899563

Following gene: 119899561

Centisome position: 82.52

GC content: 67.08

Gene sequence:

>1935_bases
ATGTGCGGAATCGCCGGCCTGTTCGATACCCGGGGCAAACGCGACTTCGACCGCGGCCTGATGTCGCGCATGAACGACAT
CCAGCATCATCGCGGCCCGGACGAGGGCGGATATCACTTCGAGCCGGGAGTTGCGCTCGCCCACCGGCGCCTGTCCATCA
TCGACCTTGCCACCGGGCAGCAGCCGCTGTTCAACGAGGACGGCTCGGTCGCGGTGGTGTTCAACGGCGAGATCTACAAC
TTCCAGGAACTGGTGCCGGAGCTTGAAGCCCTCGGCCACGTGTTCCACACCCGCAGCGACACCGAAGTCATCGTCCATGC
GTGGGAGGCCTGGGGCGAAGCCTGCGTGCACCGCTTCCGCGGCATGTTCGCCTTCGTGCTCCACGACCGTCAGCGCGAAA
CCCTGTTCCTCGCGCGCGACCGTCTCGGGGTCAAGCCGTTGTTCTATGCCCTGCTGGCCGACGGCACGCTGGCCTTCGGC
TCCGAACTGAAGGTGCTGACGCAGCACCCGGGGCTCGATCGCCGCATCGATCCGCTGGCGGTGGAGGAGTACTTCGCGCT
CGGCTACATCGCAGAGCCGCGCACCATCTTCGAAGGCGCCCGCAAGCTCGGACCGGGCGAAAGCCTTTGCATCCGGCGTG
GTCAGCCGGTGGTGGCGCCCACGCGCTACTGGGATGTCCGCTTCACGCTCGACAATCCCCTGTCGCTCGGCGACGCCTGC
GCCGAACTCACCGAACGCCTGCGCGAATCGGTGCGGCTGCGCATGATCTCCGAGGTGCCGCTGGGCGCCTTCCTGTCCGG
CGGGGTGGATTCGAGCGCGGTGGTGGCCACCATGGCAGCGCTGAGCGACGCCCCGGTAAACACCTGCTCGATCGGCTTCG
CGGATCCGCGCTTCAACGAGTCCGAGTTCGCACGCATGGTGGCGGAGCGTTATCGCACCAACCACCATGTGGAGACGGTC
GAGTCCGACGACTTCGACCTCATCGACACGCTGGCGGCGCTGTACGACGAGCCCTACGCCGACAGCTCGGCAATTCCGAC
TTACCGGGTGTGCCAGCTCGCGCGCAAACACGTCACGGTTGCGCTGTCCGGCGATGGCGGCGACGAGAGCCTTGGGGGCT
ACCGCCGCTACCGCTTGCATCTGATGGAGGAGCGGATGCGCTCGGCGCTGCCGCTGGGGGTGCGCCAGCCCCTGTTCGGG
CTGCTCGGCCGCTTGTACCCCAAGGCCGACTGGGCGCCGCGCGTGTTCCGCGCCAAGACCACCTTCCAGGCCCTCGCGCG
CGACGCGGTTGAAGCCTATTTCCACTCGGTTTCCATTCTGCGGCGCGACATGCGCAGCCAGCTGTTCTCCGCGTCCTTCC
GGGCCCAGCTCGGCGGTTACGACGCGATCGAGGTCTTCCGCCGCCACGCCGCCGCGGCCGGCACCGACGATCCGCTCGCA
TTCATCCAGTACCTCGACCTCAAGACCTACCTGGTGGGCGACATCAACACCAAGGTCGACCGCGCCAGCATGGCGCACTC
ACTGGAGGTGCGCGAGCCGCTGATGGATCATCCGCTGGTGGAGTGGCTTGCAACCTTGCCCTCGGCGCTGAAGATGAAGG
GCGGGGAGGGCAAGTACGTGTTCAAGCGCGCGATGGAGCCGATGCTGCCGCACGACGTACTGTATCGGCCCAAGATGGGT
TTTTCGGTACCGCTCGCGCGCTGGTTCCGGGGCCCGCTCAAGCAGCGCGTGCGTGAGGCGGTGCTGGGCGGCACGCTCGC
ATCCACCGGCATTTTCAACCGGCCCTACCTCGAACACCTGCTCGATGCGCATCAGTCCGGCGCGCGCGATTACTCGGCCC
CGCTGTGGACGCTGATGATGTTCGAAGCCTTCGTGCGCAATCTGGACCGCGCAGCGGCGCCGGTTGCTGCCGGCGCCGAT
CTTGCGCTCGCCTGA

Upstream 100 bases:

>100_bases
ATCAACCGTTACCACGCCCTGTACGACAACCTGCTCGCGGCGGCCGGCCAGCCGCGCGAAGCCGTCGGCAACCCGACCTG
ACGAGAGGAAGACCCTAAGC

Downstream 100 bases:

>100_bases
GGACATCCCGGTGCGCATCCTCCACATCCTCGACCATTCGCTGCCGCTGCACAGCGGCTACACCTTCCGCACCGCGGCCA
TCCTGCGCGAACAGCGCGCG

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MCGIAGLFDTRGKRDFDRGLMSRMNDIQHHRGPDEGGYHFEPGVALAHRRLSIIDLATGQQPLFNEDGSVAVVFNGEIYN
FQELVPELEALGHVFHTRSDTEVIVHAWEAWGEACVHRFRGMFAFVLHDRQRETLFLARDRLGVKPLFYALLADGTLAFG
SELKVLTQHPGLDRRIDPLAVEEYFALGYIAEPRTIFEGARKLGPGESLCIRRGQPVVAPTRYWDVRFTLDNPLSLGDAC
AELTERLRESVRLRMISEVPLGAFLSGGVDSSAVVATMAALSDAPVNTCSIGFADPRFNESEFARMVAERYRTNHHVETV
ESDDFDLIDTLAALYDEPYADSSAIPTYRVCQLARKHVTVALSGDGGDESLGGYRRYRLHLMEERMRSALPLGVRQPLFG
LLGRLYPKADWAPRVFRAKTTFQALARDAVEAYFHSVSILRRDMRSQLFSASFRAQLGGYDAIEVFRRHAAAAGTDDPLA
FIQYLDLKTYLVGDINTKVDRASMAHSLEVREPLMDHPLVEWLATLPSALKMKGGEGKYVFKRAMEPMLPHDVLYRPKMG
FSVPLARWFRGPLKQRVREAVLGGTLASTGIFNRPYLEHLLDAHQSGARDYSAPLWTLMMFEAFVRNLDRAAAPVAAGAD
LALA

Sequences:

>Translated_644_residues
MCGIAGLFDTRGKRDFDRGLMSRMNDIQHHRGPDEGGYHFEPGVALAHRRLSIIDLATGQQPLFNEDGSVAVVFNGEIYN
FQELVPELEALGHVFHTRSDTEVIVHAWEAWGEACVHRFRGMFAFVLHDRQRETLFLARDRLGVKPLFYALLADGTLAFG
SELKVLTQHPGLDRRIDPLAVEEYFALGYIAEPRTIFEGARKLGPGESLCIRRGQPVVAPTRYWDVRFTLDNPLSLGDAC
AELTERLRESVRLRMISEVPLGAFLSGGVDSSAVVATMAALSDAPVNTCSIGFADPRFNESEFARMVAERYRTNHHVETV
ESDDFDLIDTLAALYDEPYADSSAIPTYRVCQLARKHVTVALSGDGGDESLGGYRRYRLHLMEERMRSALPLGVRQPLFG
LLGRLYPKADWAPRVFRAKTTFQALARDAVEAYFHSVSILRRDMRSQLFSASFRAQLGGYDAIEVFRRHAAAAGTDDPLA
FIQYLDLKTYLVGDINTKVDRASMAHSLEVREPLMDHPLVEWLATLPSALKMKGGEGKYVFKRAMEPMLPHDVLYRPKMG
FSVPLARWFRGPLKQRVREAVLGGTLASTGIFNRPYLEHLLDAHQSGARDYSAPLWTLMMFEAFVRNLDRAAAPVAAGAD
LALA
>Mature_644_residues
MCGIAGLFDTRGKRDFDRGLMSRMNDIQHHRGPDEGGYHFEPGVALAHRRLSIIDLATGQQPLFNEDGSVAVVFNGEIYN
FQELVPELEALGHVFHTRSDTEVIVHAWEAWGEACVHRFRGMFAFVLHDRQRETLFLARDRLGVKPLFYALLADGTLAFG
SELKVLTQHPGLDRRIDPLAVEEYFALGYIAEPRTIFEGARKLGPGESLCIRRGQPVVAPTRYWDVRFTLDNPLSLGDAC
AELTERLRESVRLRMISEVPLGAFLSGGVDSSAVVATMAALSDAPVNTCSIGFADPRFNESEFARMVAERYRTNHHVETV
ESDDFDLIDTLAALYDEPYADSSAIPTYRVCQLARKHVTVALSGDGGDESLGGYRRYRLHLMEERMRSALPLGVRQPLFG
LLGRLYPKADWAPRVFRAKTTFQALARDAVEAYFHSVSILRRDMRSQLFSASFRAQLGGYDAIEVFRRHAAAAGTDDPLA
FIQYLDLKTYLVGDINTKVDRASMAHSLEVREPLMDHPLVEWLATLPSALKMKGGEGKYVFKRAMEPMLPHDVLYRPKMG
FSVPLARWFRGPLKQRVREAVLGGTLASTGIFNRPYLEHLLDAHQSGARDYSAPLWTLMMFEAFVRNLDRAAAPVAAGAD
LALA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=393, Percent_Identity=29.2620865139949, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI168229252, Length=393, Percent_Identity=29.2620865139949, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI168229250, Length=393, Percent_Identity=29.2620865139949, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI296010848, Length=363, Percent_Identity=28.9256198347107, Blast_Score=104, Evalue=3e-22,
Organism=Escherichia coli, GI1786889, Length=418, Percent_Identity=29.6650717703349, Blast_Score=139, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI71993933, Length=390, Percent_Identity=32.5641025641026, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25147557, Length=390, Percent_Identity=32.5641025641026, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25147560, Length=326, Percent_Identity=32.8220858895706, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560178, Length=407, Percent_Identity=29.2383292383292, Blast_Score=109, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321563, Length=422, Percent_Identity=27.9620853080569, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6325403, Length=425, Percent_Identity=27.0588235294118, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI45553209, Length=403, Percent_Identity=32.0099255583127, Blast_Score=139, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72007; Mature: 72007

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGLFDTRGKRDFDRGLMSRMNDIQHHRGPDEGGYHFEPGVALAHRRLSIIDLATGQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHEEEEEECCCC
QPLFNEDGSVAVVFNGEIYNFQELVPELEALGHVFHTRSDTEVIVHAWEAWGEACVHRFR
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
GMFAFVLHDRQRETLFLARDRLGVKPLFYALLADGTLAFGSELKVLTQHPGLDRRIDPLA
HHHHHHHHCCCHHHEEEEHHCCCCHHHHHHHHHCCCCCCCCCCHHEECCCCCCCCCCCHH
VEEYFALGYIAEPRTIFEGARKLGPGESLCIRRGQPVVAPTRYWDVRFTLDNPLSLGDAC
HHHHHHHHCCCCCHHHHHHHHHCCCCCHHHEECCCCCCCCCEEEEEEEEECCCCCHHHHH
AELTERLRESVRLRMISEVPLGAFLSGGVDSSAVVATMAALSDAPVNTCSIGFADPRFNE
HHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCH
SEFARMVAERYRTNHHVETVESDDFDLIDTLAALYDEPYADSSAIPTYRVCQLARKHVTV
HHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCEEE
ALSGDGGDESLGGYRRYRLHLMEERMRSALPLGVRQPLFGLLGRLYPKADWAPRVFRAKT
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
TFQALARDAVEAYFHSVSILRRDMRSQLFSASFRAQLGGYDAIEVFRRHAAAAGTDDPLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHH
FIQYLDLKTYLVGDINTKVDRASMAHSLEVREPLMDHPLVEWLATLPSALKMKGGEGKYV
HHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHCCCCCCHHH
FKRAMEPMLPHDVLYRPKMGFSVPLARWFRGPLKQRVREAVLGGTLASTGIFNRPYLEHL
HHHHCCCCCCHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCHHHHCCCCCCHHHHHH
LDAHQSGARDYSAPLWTLMMFEAFVRNLDRAAAPVAAGADLALA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MCGIAGLFDTRGKRDFDRGLMSRMNDIQHHRGPDEGGYHFEPGVALAHRRLSIIDLATGQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHEEEEEECCCC
QPLFNEDGSVAVVFNGEIYNFQELVPELEALGHVFHTRSDTEVIVHAWEAWGEACVHRFR
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
GMFAFVLHDRQRETLFLARDRLGVKPLFYALLADGTLAFGSELKVLTQHPGLDRRIDPLA
HHHHHHHHCCCHHHEEEEHHCCCCHHHHHHHHHCCCCCCCCCCHHEECCCCCCCCCCCHH
VEEYFALGYIAEPRTIFEGARKLGPGESLCIRRGQPVVAPTRYWDVRFTLDNPLSLGDAC
HHHHHHHHCCCCCHHHHHHHHHCCCCCHHHEECCCCCCCCCEEEEEEEEECCCCCHHHHH
AELTERLRESVRLRMISEVPLGAFLSGGVDSSAVVATMAALSDAPVNTCSIGFADPRFNE
HHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCH
SEFARMVAERYRTNHHVETVESDDFDLIDTLAALYDEPYADSSAIPTYRVCQLARKHVTV
HHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCEEE
ALSGDGGDESLGGYRRYRLHLMEERMRSALPLGVRQPLFGLLGRLYPKADWAPRVFRAKT
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
TFQALARDAVEAYFHSVSILRRDMRSQLFSASFRAQLGGYDAIEVFRRHAAAAGTDDPLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHH
FIQYLDLKTYLVGDINTKVDRASMAHSLEVREPLMDHPLVEWLATLPSALKMKGGEGKYV
HHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHCCCCCCHHH
FKRAMEPMLPHDVLYRPKMGFSVPLARWFRGPLKQRVREAVLGGTLASTGIFNRPYLEHL
HHHHCCCCCCHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCHHHHCCCCCCHHHHHH
LDAHQSGARDYSAPLWTLMMFEAFVRNLDRAAAPVAAGADLALA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]