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The map label for this gene is dnaG [H]

Identifier: 119899515

GI number: 119899515

Start: 3555250

End: 3557070

Strand: Direct

Name: dnaG [H]

Synonym: azo3226

Alternate gene names: 119899515

Gene position: 3555250-3557070 (Clockwise)

Preceding gene: 119899514

Following gene: 119899516

Centisome position: 81.24

GC content: 66.06

Gene sequence:

>1821_bases
ATGATTCCACAAGCTTTCGTCCAGGACCTGTTGTCCAGGATCGACATCGTCGACGTCATCGAACGCTACCTGCCCCTCAA
GAAGGGCGGCGCGAACTACTTCGCCTGCTGCCCCTTTCACGGGGAGAACTCCGCCTCGTTCTCGGTCAGCCCGACCAAGC
AGTTTTACCATTGCTTCGGCTGCGGCGCACACGGCAGCGCGATCGGCTTTCTCATGGAATACAGCGGGCTCGGCTTCGTC
GACGCCGTGAAGGAACTGGCCAACCAGGCCGGCATGCAGGTACCTGACGACGGCCAACGGGTAAACGCCGCGCAAAGCGG
GGAACACGACAGGCTGTTCGACGCGGTAGCCGCCGCGGCCCGTTTCTACCGCGACGCGCTCAAGGCCGCACCCATCGCCA
TCGATTATCTGAAGCGACGCGGACTCAGCGGCGAAGTCGCCGCCCGCTTCGGCATCGGCTATGCGCCGGACGACTGGCAG
GCGCTGCAGAAGGTCTTTCCGGACTACCAAGCAAAGACCCTCGCCGACGCCGGACTGGTCATCGACAACGACCAGGGTCG
GCGTTATGACCGCTTCCGCGGCAGGGTGATGTTCCCGATCCACGACCGCCGCGGACGCATCATTGCCTTCGGCGGGCGCG
TACTGGATCAGGGCGAACCCAAATACCTGAACTCGCCCGAGACCCCGCTGTTCGAGAAGGGGCGCGAACTCTACGGCCTG
TTTCTGGCGCAGAAGGCCATCCGCGACGCCGGCTTTGCCTTGGTGGTGGAAGGCTACATGGACGTCGTCGCGCTCGCGCA
GTTCGGCATCGAGAATGCCGTCGCCACGCTCGGCACGGCCACCACGACGCATCACATCCAGGCGCTGCTGCGTCACACCG
ACCGCGTGGTGTTCTGCTTTGACGGCGATAACGCCGGCCGCCGCGCAGCGCGACGCGCGCTGGAGAATGCCCTCGACGCC
CTGCGCGACGACGTCACCCTCGCCTTCCTGTTTCTGCCCACGGAGCACGATCCGGATTCTTTCGTACGCAGTGAAGGAGC
CGCCGCATTCCGCCAAGCCGCAGCTGAAGCCACGCCGCTCGCACGGACCCTGCTCGACCAGCTTCAGGAGGACCTCGACC
TCGCGACCGCCGAAGGCCGTGCGCGCCTGGTCCACGACGCCCGTCCGCTGGTCACCCGGATCCCGGCGCCACTGCTGAAA
CTGCAGTTGATCAAAGCCGTTGCGGAGGCCAGCCAGCTTACGCAGGCTGAAGTGGAACATGCGTTCGGCCTGGCCGGTCC
CCAGGCGGGAACGAGCCCGCCCCGCGCCGACTCACCCCGCCCGGGACCGCGGCGCCCATCGGGCTTCAGCCGTCCGGCGC
GCGGCCCCGGCCGCCGCGCACCCTCGACCACCGCAACGCTTCTCCGTCTCGTGGTTCACCACCCGGCGTTGGCAGCGCGC
CTGCCGGCCGATCTACTGCCGGCCGAAACCCCCGAAAGCCGCGCACTTGTCGCCATCGTGGATCTGGTAAGCCTGGGCGA
ACCCCTGCCCGCAGGCGGCGTCGGAGGACTTATCGAACGCTTTCGCGACACCCCGCACGCGGAGACACTCTCGCGGGTTG
CCGCCGAACTGATCGACACCGAGTTTGACGACAGCGCGATCGAAACGCTTTTCGAAGACGCCTTGCGCAAACTGGGCGCG
GATGCTGTAACGAGAGAAATCGACGCGCTGCTCGCCCTGGAGCGTGAGCAAGGCCTCTCGACCGCCGAACGGCATCGCCT
CAGCGAACTCATCCTGGAGCGCCGGAACCTCGCCAATGTAGGCAAAGTCTCAGATTTATAG

Upstream 100 bases:

>100_bases
ATGCTGCCGCCGAAGCTCTACTGATTCAGCCGCCACTCGGTCGTCTGCACGCCGGACGGAAGCCCCTCGGGCCTCCGTCC
GGCGTATCGCTTTTTACTCG

Downstream 100 bases:

>100_bases
TACAATTTCCTGTTTCCCATTCTCATCTGCCCCAGATTCGAGGAGCGCCATGGCACGCGAAAAAGCCGCCAAAGATTCGC
GCAAGGACACCCCCAAAGGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MIPQAFVQDLLSRIDIVDVIERYLPLKKGGANYFACCPFHGENSASFSVSPTKQFYHCFGCGAHGSAIGFLMEYSGLGFV
DAVKELANQAGMQVPDDGQRVNAAQSGEHDRLFDAVAAAARFYRDALKAAPIAIDYLKRRGLSGEVAARFGIGYAPDDWQ
ALQKVFPDYQAKTLADAGLVIDNDQGRRYDRFRGRVMFPIHDRRGRIIAFGGRVLDQGEPKYLNSPETPLFEKGRELYGL
FLAQKAIRDAGFALVVEGYMDVVALAQFGIENAVATLGTATTTHHIQALLRHTDRVVFCFDGDNAGRRAARRALENALDA
LRDDVTLAFLFLPTEHDPDSFVRSEGAAAFRQAAAEATPLARTLLDQLQEDLDLATAEGRARLVHDARPLVTRIPAPLLK
LQLIKAVAEASQLTQAEVEHAFGLAGPQAGTSPPRADSPRPGPRRPSGFSRPARGPGRRAPSTTATLLRLVVHHPALAAR
LPADLLPAETPESRALVAIVDLVSLGEPLPAGGVGGLIERFRDTPHAETLSRVAAELIDTEFDDSAIETLFEDALRKLGA
DAVTREIDALLALEREQGLSTAERHRLSELILERRNLANVGKVSDL

Sequences:

>Translated_606_residues
MIPQAFVQDLLSRIDIVDVIERYLPLKKGGANYFACCPFHGENSASFSVSPTKQFYHCFGCGAHGSAIGFLMEYSGLGFV
DAVKELANQAGMQVPDDGQRVNAAQSGEHDRLFDAVAAAARFYRDALKAAPIAIDYLKRRGLSGEVAARFGIGYAPDDWQ
ALQKVFPDYQAKTLADAGLVIDNDQGRRYDRFRGRVMFPIHDRRGRIIAFGGRVLDQGEPKYLNSPETPLFEKGRELYGL
FLAQKAIRDAGFALVVEGYMDVVALAQFGIENAVATLGTATTTHHIQALLRHTDRVVFCFDGDNAGRRAARRALENALDA
LRDDVTLAFLFLPTEHDPDSFVRSEGAAAFRQAAAEATPLARTLLDQLQEDLDLATAEGRARLVHDARPLVTRIPAPLLK
LQLIKAVAEASQLTQAEVEHAFGLAGPQAGTSPPRADSPRPGPRRPSGFSRPARGPGRRAPSTTATLLRLVVHHPALAAR
LPADLLPAETPESRALVAIVDLVSLGEPLPAGGVGGLIERFRDTPHAETLSRVAAELIDTEFDDSAIETLFEDALRKLGA
DAVTREIDALLALEREQGLSTAERHRLSELILERRNLANVGKVSDL
>Mature_606_residues
MIPQAFVQDLLSRIDIVDVIERYLPLKKGGANYFACCPFHGENSASFSVSPTKQFYHCFGCGAHGSAIGFLMEYSGLGFV
DAVKELANQAGMQVPDDGQRVNAAQSGEHDRLFDAVAAAARFYRDALKAAPIAIDYLKRRGLSGEVAARFGIGYAPDDWQ
ALQKVFPDYQAKTLADAGLVIDNDQGRRYDRFRGRVMFPIHDRRGRIIAFGGRVLDQGEPKYLNSPETPLFEKGRELYGL
FLAQKAIRDAGFALVVEGYMDVVALAQFGIENAVATLGTATTTHHIQALLRHTDRVVFCFDGDNAGRRAARRALENALDA
LRDDVTLAFLFLPTEHDPDSFVRSEGAAAFRQAAAEATPLARTLLDQLQEDLDLATAEGRARLVHDARPLVTRIPAPLLK
LQLIKAVAEASQLTQAEVEHAFGLAGPQAGTSPPRADSPRPGPRRPSGFSRPARGPGRRAPSTTATLLRLVVHHPALAAR
LPADLLPAETPESRALVAIVDLVSLGEPLPAGGVGGLIERFRDTPHAETLSRVAAELIDTEFDDSAIETLFEDALRKLGA
DAVTREIDALLALEREQGLSTAERHRLSELILERRNLANVGKVSDL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=599, Percent_Identity=43.7395659432387, Blast_Score=444, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65838; Mature: 65838

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPQAFVQDLLSRIDIVDVIERYLPLKKGGANYFACCPFHGENSASFSVSPTKQFYHCFG
CCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEECCCHHHHHHHHC
CGAHGSAIGFLMEYSGLGFVDAVKELANQAGMQVPDDGQRVNAAQSGEHDRLFDAVAAAA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RFYRDALKAAPIAIDYLKRRGLSGEVAARFGIGYAPDDWQALQKVFPDYQAKTLADAGLV
HHHHHHHHHCHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHCCCE
IDNDQGRRYDRFRGRVMFPIHDRRGRIIAFGGRVLDQGEPKYLNSPETPLFEKGRELYGL
EECCCCCHHHHHCCEEEEEEECCCCCEEEECCEEECCCCCCCCCCCCCCHHHCCHHHHHH
FLAQKAIRDAGFALVVEGYMDVVALAQFGIENAVATLGTATTTHHIQALLRHTDRVVFCF
HHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEE
DGDNAGRRAARRALENALDALRDDVTLAFLFLPTEHDPDSFVRSEGAAAFRQAAAEATPL
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHH
ARTLLDQLQEDLDLATAEGRARLVHDARPLVTRIPAPLLKLQLIKAVAEASQLTQAEVEH
HHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AFGLAGPQAGTSPPRADSPRPGPRRPSGFSRPARGPGRRAPSTTATLLRLVVHHPALAAR
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
LPADLLPAETPESRALVAIVDLVSLGEPLPAGGVGGLIERFRDTPHAETLSRVAAELIDT
CCHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC
EFDDSAIETLFEDALRKLGADAVTREIDALLALEREQGLSTAERHRLSELILERRNLANV
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
GKVSDL
CCCCCC
>Mature Secondary Structure
MIPQAFVQDLLSRIDIVDVIERYLPLKKGGANYFACCPFHGENSASFSVSPTKQFYHCFG
CCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEECCCHHHHHHHHC
CGAHGSAIGFLMEYSGLGFVDAVKELANQAGMQVPDDGQRVNAAQSGEHDRLFDAVAAAA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RFYRDALKAAPIAIDYLKRRGLSGEVAARFGIGYAPDDWQALQKVFPDYQAKTLADAGLV
HHHHHHHHHCHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHCCCE
IDNDQGRRYDRFRGRVMFPIHDRRGRIIAFGGRVLDQGEPKYLNSPETPLFEKGRELYGL
EECCCCCHHHHHCCEEEEEEECCCCCEEEECCEEECCCCCCCCCCCCCCHHHCCHHHHHH
FLAQKAIRDAGFALVVEGYMDVVALAQFGIENAVATLGTATTTHHIQALLRHTDRVVFCF
HHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEE
DGDNAGRRAARRALENALDALRDDVTLAFLFLPTEHDPDSFVRSEGAAAFRQAAAEATPL
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHH
ARTLLDQLQEDLDLATAEGRARLVHDARPLVTRIPAPLLKLQLIKAVAEASQLTQAEVEH
HHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AFGLAGPQAGTSPPRADSPRPGPRRPSGFSRPARGPGRRAPSTTATLLRLVVHHPALAAR
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
LPADLLPAETPESRALVAIVDLVSLGEPLPAGGVGGLIERFRDTPHAETLSRVAAELIDT
CCHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC
EFDDSAIETLFEDALRKLGADAVTREIDALLALEREQGLSTAERHRLSELILERRNLANV
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
GKVSDL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]